Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,070,464 T→G 5.6% intergenic (‑63/+51) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,070,4640TG 5.6% 1588.2 / 28.0 492intergenic (‑63/+51)yihR/yihSputative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base T (329/120);  new base G (3/34);  total (332/160)
Fisher's exact test for biased strand distribution p-value = 2.15e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 507 total aligned reads displayed.

GTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATA  >  NC_000913/4070315‑4070613
                                                                                                                                                      |                                                                                                                                                      
gTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCttt                                                                                                                                                        >  1:2837368/1‑150 (MQ=255)
  gATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                                                                      >  2:7952609/1‑150 (MQ=255)
  gATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                                                                                      <  1:8588603/150‑1 (MQ=255)
  gATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGCCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTgg                                                                                                                                                      >  2:2670473/1‑150 (MQ=255)
     aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                                                                                   >  1:3999275/1‑150 (MQ=255)
     aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                                                                                   <  2:4583665/150‑1 (MQ=255)
       tCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                                                                                 >  1:5916292/1‑150 (MQ=255)
       tCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATGTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                                                                                 <  1:2520103/150‑1 (MQ=255)
        cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                                                                                <  1:3698615/150‑1 (MQ=255)
         ccccGCGGCACGTGAAACGGGCTGTCCACTGCAATGCATCTTGGTGATTTGCCTACCAGGCACCTTAATTACCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCACCAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                                                                               <  2:7033576/150‑1 (MQ=255)
         ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCTCCCCAAAGGGGCGAgg                                                                                                                                               <  1:8791459/150‑1 (MQ=255)
         ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAgg                                                                                                                                               <  1:446216/150‑1 (MQ=255)
           ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                                                                                             >  1:2603708/1‑150 (MQ=255)
            cgcgGCACGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTATCGACATACAGCCTCCCGTGAGGCGGATGTCTAACCCAATAACTGCACCGGGCGGTCCCCTCGCCCCGTTGGGGAGAGGGtt                                                                                                                                            >  1:1201604/1‑150 (MQ=255)
             gcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTa                                                                                                                                           <  2:5241000/150‑1 (MQ=255)
                gCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAggg                                                                                                                                        >  2:2957382/1‑150 (MQ=255)
                 cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCCCCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTGGGGAGAGGGTTAGGga                                                                                                                                      >  1:6194660/1‑150 (MQ=255)
                 cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGga                                                                                                                                      <  2:4167977/150‑1 (MQ=255)
                 cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGga                                                                                                                                      <  2:1293405/150‑1 (MQ=255)
                   aGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgaga                                                                                                                                    <  2:7495244/150‑1 (MQ=255)
                    gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                                   >  2:6771680/1‑150 (MQ=255)
                    gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGagag                                                                                                                                   <  2:7721031/150‑1 (MQ=255)
                     tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgg                                                                                                                                  >  1:638578/1‑150 (MQ=255)
                      gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAggg                                                                                                                                 <  2:4844843/150‑1 (MQ=255)
                      gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAggg                                                                                                                                 >  1:564080/1‑150 (MQ=255)
                       aaaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgggg                                                                                                                                >  2:3504104/1‑150 (MQ=255)
                         aCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGaa                                                                                                                              >  2:1848446/1‑150 (MQ=255)
                                 tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCTTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                                                                                      >  2:6908337/1‑150 (MQ=255)
                                 tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                                                                                      >  1:1668735/1‑150 (MQ=255)
                                 tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                                                                                      >  2:4437065/1‑150 (MQ=255)
                                 tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                                                                                      >  2:895789/1‑150 (MQ=255)
                                   cACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAcc                                                                                                                    >  1:10023170/1‑150 (MQ=255)
                                    aCTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTTAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCAccc                                                                                                                   >  1:1075386/1‑150 (MQ=255)
                                      tGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCATCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTa                                                                                                                 >  2:955732/1‑150 (MQ=255)
                                        cAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtt                                                                                                               >  2:1151497/1‑150 (MQ=255)
                                                ttgggTGATTGGCATACATGGCACCTTATTTAACGACATACAGCCTCCGGTGTGGGTGATGTAAAACCAAAAACCTGCACCGGAGGGGCCCCTCGCCCATTTGGGGAGTGGGTTCGGGATCGGGGAAAACCCGCACCCTATTTTCAGATc                                                                                                       <  2:6655103/147‑1 (MQ=255)
                                                 ttGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCa                                                                                                      >  1:9416425/1‑150 (MQ=255)
                                                 tgggttttttGAATACATGGCACATTAATTAACTACACACAGCCTCCGGTGCGGCTGCTGAAAAACCCAAAAACTGCCCGGGAGGGTCCCCCCGCCCCTTTGGGGCGCGGGTTTGGGTGTGGGGAAAACCCGCACCCTATTTTCAGATCa                                                                                                      <  2:830979/143‑1 (MQ=255)
                                                    gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGtt                                                                                                   >  1:6281014/1‑150 (MQ=255)
                                                    gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGtt                                                                                                   >  1:9253392/1‑150 (MQ=255)
                                                      gATTTGCATACACGGCACCTTTATTAACGACATACCGCCTCCGGTGCGGCTGTTTTCAACCCCAAAACCTGCACCGGCGGGTCCCCTCGCCCCTTTGGGGTGTGGGTTCGGGAGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAt                                                                                                 <  2:10231527/150‑1 (MQ=255)
                                                       aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAtt                                                                                                >  1:6669959/1‑150 (MQ=255)
                                                         ttGAATACAGGGCACATTAATTCACGACAAACCGCCTCCGGTGTGGCTGTTGCAAAACCAAAACCCGGCCCGGGCGGGGCCCCGCGCCCGTTGGGGGTGTGGTTTTGGGTGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTc                                                                                              <  2:5083701/150‑1 (MQ=255)
                                                           gCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc                                                                                            >  1:8206089/1‑150 (MQ=255)
                                                                 aaGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat                                                                                      >  1:2432200/1‑150 (MQ=255)
                                                                 aaGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat                                                                                      >  1:5463463/1‑150 (MQ=255)
                                                                 aaGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCGTTGGGGAGAGGGTTAGGGAGAGGGGAAACCCGCACCCTATTTTCAGATCAGTTATTTCACATGAata                                                                                     >  1:7124146/1‑150 (MQ=255)
                                                                  aGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAata                                                                                     >  2:555529/1‑150 (MQ=255)
                                                                    gccccTTAATTAACGACATCCCGCCGCCGGTGTGGGTGTGGTCAACCCCAAACCCGGCCCGGGCGGGGCCCCGGGCCCGTTTGGGGTGGGGGTTGGGGTGGGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATc                                                                                   <  2:1454703/147‑1 (MQ=255)
                                                                    gCACCTTAATTAACGCCATCCAGCCTCCGGTGTGGGTGATGTCAAACCCAAAACCGGCCCCGGCGGGGCCCCGCGCCCTTTTGGGGTGTGGGTTCGGGTGAGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATc                                                                                   <  2:9353026/150‑1 (MQ=255)
                                                                    gCACCTTAATTAACGACATCCCGCCGCCCTTGCGGCTGATGCCAACCCCAAAAACGGCCCCGGACGGTCCCCCCCCCCCTTGGGGTTGTGGTTTTGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATc                                                                                   <  2:2344781/150‑1 (MQ=255)
                                                                    gCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATc                                                                                   >  2:8216747/1‑150 (MQ=255)
                                                                     cACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATcc                                                                                  >  2:3446966/1‑150 (MQ=255)
                                                                      cccTTAATTAACGACAGACAGCCCCCCGTGAGGCTGATTTCAACCCCAAACACGGCACCGGACGGGCCCCCCGCCCCTTTGGGGAGCGGTTTAGGGAGAGGGTAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca                                                                                 <  2:7584591/149‑1 (MQ=255)
                                                                       aattaataaaCCAAATACAGCCCCCCTTTAGGCTTATTAAAAACCCAAAAACTCCACCGCACGGCCCCCTCCCCCCTTTGGGTAGCGGTTTAGGCAGAGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                                                                                <  2:9148978/148‑1 (MQ=255)
                                                                        cTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc                                                                               >  2:4254262/1‑150 (MQ=255)
                                                                        cTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc                                                                               >  1:4731008/1‑150 (MQ=255)
                                                                         ttaattaaCGCCACACATCCTCCCGTGTGGCTGATGTAAAACCCAAAAACTGCCCCGGAGGTTCCCCTCCCCCTTTTGGGTAGTGGTTTAGGGAGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagca                                                                              <  2:6641541/150‑1 (MQ=255)
                                                                         ttaattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagag                                                                              >  1:2292650/1‑148 (MQ=255)
                                                                          taattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGCCGGACCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTGATTTCGCATTAATATCcagcag                                                                             >  2:2112160/1‑150 (MQ=255)
                                                                           aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGa                                                                            >  1:8315251/1‑150 (MQ=255)
                                                                                aCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGc                                                                       >  1:4161377/1‑150 (MQ=255)
                                                                                aCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGc                                                                       >  1:4554289/1‑150 (MQ=255)
                                                                                  gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                                                                                 <  1:9609997/75‑1 (MQ=255)
                                                                                  gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                                                                                 <  1:7769869/75‑1 (MQ=255)
                                                                                  gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTCTTTCGCGTTGATATCCAGCAGACCCGCTg                                                                     >  2:9727136/1‑150 (MQ=255)
                                                                                  gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTg                                                                     >  1:10136043/1‑150 (MQ=255)
                                                                                    cATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa                                                                   >  1:2039228/1‑150 (MQ=255)
                                                                                         aGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAACACCCGCACCCTATTTTCAGCTCAGTTATTTCGCAGTAATATCCAGCAGACCCGCTGCACCCGc                                                              >  1:341681/1‑150 (MQ=255)
                                                                                                cgggAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCGTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCACCCGCTGGAGcc                                                       >  2:8862735/4‑150 (MQ=255)
                                                                                                   gAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCTCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATg                                                    >  1:9872155/1‑150 (MQ=255)
                                                                                                    aGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGc                                                   >  1:5828689/1‑150 (MQ=255)
                                                                                                    aGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGc                                                   >  1:3557725/1‑150 (MQ=255)
                                                                                                      gCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCg                                                 >  2:1031785/1‑150 (MQ=255)
                                                                                                         gatgaaaaaCCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGTATTGGGTTAGGTAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGgc                                              <  2:2516724/144‑1 (MQ=255)
                                                                                                           ttccaaaccccaaacccGCCCCGGCCGGGCCCCCCCCCCCGTTGGGGTTGGGGTTTGGGTCGGGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGgcg                                             <  2:7198277/147‑1 (MQ=255)
                                                                                                             tCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTa                                          >  1:3000804/1‑150 (MQ=255)
                                                                                                             tCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTa                                          >  2:8759831/1‑150 (MQ=255)
                                                                                                               aaaCCCAACAACTGCACCGCTCGGCCCCCCCGCCCATTGGGGGTGTGGTTTTGGGTGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGTCGGGCGCTAAc                                        <  2:8525642/150‑1 (MQ=255)
                                                                                                                aaCCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACg                                       >  2:1661751/1‑150 (MQ=255)
                                                                                                                 aCCAAAAACCTGCACGGCAGGGCCCCCCCGCCCTTTGGGGGTGTGGGTTCGGGAGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACgg                                      <  2:5444172/150‑1 (MQ=255)
                                                                                                                    cAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGAt                                   >  2:6269025/1‑150 (MQ=255)
                                                                                                                    cAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGAt                                   >  2:3620134/1‑150 (MQ=255)
                                                                                                                          cTGCCCCGGAGGGCCCCCCCGCCCCTTGGGGGTGCGGGTTCGGGCGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCgg                             <  2:10023170/150‑1 (MQ=255)
                                                                                                                             cACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGGGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGAt                          <  2:3402464/150‑1 (MQ=255)
                                                                                                                             cACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGAt                          >  1:7976579/1‑150 (MQ=255)
                                                                                                                              aCCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATc                         >  1:4255300/1‑150 (MQ=255)
                                                                                                                                cgcaCGGACCCCCCCCCCCTTTGGGTTGTGGTTTTGGTTGTGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCAc                       <  2:617330/147‑1 (MQ=255)
                                                                                                                                cGGACGGTCCCCGCGCCCCTTTGGGGTGTGGGTTCGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCAc                       <  2:6410104/150‑1 (MQ=255)
                                                                                                                                 cggcgggTCCCCTCGCCCCTTTGGGTTGCGGGTTAGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCAc                       <  2:5676766/145‑1 (MQ=255)
                                                                                                                                          cccTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATTCGCGGGATCACCAGGCAATg              >  1:325085/1‑150 (MQ=255)
                                                                                                                                          cccTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATg              >  1:7614154/1‑150 (MQ=255)
                                                                                                                                           ccTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgc             >  2:5892607/1‑150 (MQ=255)
                                                                                                                                            cTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCACTgca            >  1:7338359/1‑150 (MQ=255)
                                                                                                                                            cTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgca            >  1:1423771/1‑150 (MQ=255)
                                                                                                                                             tCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgcag           >  1:5014227/1‑150 (MQ=255)
                                                                                                                                                   cTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTg     >  1:6968975/1‑150 (MQ=255)
                                                                                                                                                    tttGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGa    >  1:5309739/1‑150 (MQ=255)
                                                                                                                                                    tttGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGa    >  1:1852263/1‑150 (MQ=255)
                                                                                                                                                      tggggtggggTTTGGGGAGTGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATa  <  1:517527/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATA  >  NC_000913/4070315‑4070613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: