Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 126,093 T→C 11.1% A133A (GCT→GCC aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,0930TC11.1% 228.2 / 10.9 88A133A (GCT→GCCaceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base T (35/43);  new base C (5/5);  total (40/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGT  >  NC_000913/125945‑126237
                                                                                                                                                    |                                                                                                                                                
cACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCt                                                                                                                                                  >  1:628613/1‑149 (MQ=255)
 aCCTGCTCAGGCAGAAGAGAAGAAAGACGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                                 >  1:891258/1‑149 (MQ=255)
 aCCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                                 >  1:924295/1‑149 (MQ=255)
  ccTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa                                                                                                                                                <  1:203813/149‑1 (MQ=255)
    tGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc                                                                                                                                              >  2:313347/1‑149 (MQ=255)
      cTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                                                                                                                            >  1:344298/1‑149 (MQ=255)
           gCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTAAACGTTCCGGATATCGGCAGCGACGAAGATGAAGTGACCGAAATCATGGTGAAAGATGGCGATAAAGATGAAGCTGAACAgaagct                                                                                                                                       >  2:890505/1‑144 (MQ=255)
              gaagagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGAt                                                                                                                                    >  1:8884/1‑149 (MQ=255)
               aagagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc                                                                                                                                   <  2:899383/149‑1 (MQ=255)
                 gagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAACACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAc                                                                                                                                 >  2:868621/1‑149 (MQ=255)
                   gcagaaagcaGCAGCGCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGCAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCg                                                                                                                               <  2:666429/147‑1 (MQ=255)
                    aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt                                                                                                                              >  1:144026/1‑149 (MQ=255)
                    aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTGCCGGAGATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt                                                                                                                              <  1:963085/149‑1 (MQ=255)
                         agaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg                                                                                                                         >  1:541299/1‑149 (MQ=255)
                          gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAgg                                                                                                                        <  2:203140/149‑1 (MQ=255)
                           aagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                                                                                                                       <  1:978747/149‑1 (MQ=255)
                              cagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc                                                                                                                    >  2:378963/1‑149 (MQ=255)
                               agcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGAAGCGACGAAGTTGAAGTGACCGAAATCATGGTGAAATTTGGCGATAAAGTTGACGCTGAACACTCGCTGATCACCGTAGAAGgagaaa                                                                                                                   >  2:670595/1‑147 (MQ=25)
                                   ccGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGc                                                                                                               >  2:307784/1‑149 (MQ=255)
                                     ggCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt                                                                                                             <  1:246728/149‑1 (MQ=255)
                                           cgcaCCAGCGGCTGCGGCGGCAAACGACGTTCACGTTCCGGATATCGGCAGCGCCGAAGTGGCAGTGACCGAAATCCTGGGGAAAGTTGGCGATAACGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATgg                                                                                                       <  1:154920/148‑1 (MQ=25)
                                           cgaaCCAGCGGCTGGGTCGGCAAAAGACGTTAACGTTCCGGATCTCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg                                                                                                       <  2:210922/146‑1 (MQ=255)
                                             caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGCCGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATAACCGTAGAATGAGACAATTCTTCTATGGaa                                                                                                     >  1:57870/1‑149 (MQ=21)
                                             caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa                                                                                                     <  2:319986/149‑1 (MQ=255)
                                               ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt                                                                                                   <  2:801983/149‑1 (MQ=255)
                                                 aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTc                                                                                                 <  1:299594/149‑1 (MQ=255)
                                                  gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTcc                                                                                                >  2:917356/1‑149 (MQ=255)
                                                  gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGAGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGTAAGTTcc                                                                                                >  2:561428/1‑149 (MQ=255)
                                                   cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCg                                                                                               <  1:966812/149‑1 (MQ=255)
                                                        gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc                                                                                          <  2:855034/149‑1 (MQ=255)
                                                          ggcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGt                                                                                        <  1:868621/149‑1 (MQ=255)
                                                              gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGc                                                                                    <  1:478807/149‑1 (MQ=255)
                                                                        ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg                                                                          >  2:348526/1‑149 (MQ=255)
                                                                           aCGTTACGGTTATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAATGTTGAAGCTGAACAGTCGCTGTTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTGCGTTTGCTGGCACCGTGaaa                                                                       <  1:890505/149‑1 (MQ=255)
                                                                              ttCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag                                                                    <  2:611524/149‑1 (MQ=255)
                                                                               tCCGGATATCGGCAGCGACGAAGATGAAGTGAACGAAAGCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga                                                                   <  1:391583/149‑1 (MQ=255)
                                                                                ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt                                                                  <  2:924295/149‑1 (MQ=255)
                                                                                ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt                                                                  <  2:8884/149‑1 (MQ=255)
                                                                                    atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATTGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaaa                                                              >  2:957447/1‑149 (MQ=255)
                                                                                       tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGTGATCAAAGTg                                                           <  1:980505/149‑1 (MQ=255)
                                                                                          gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAAc                                                        >  1:755980/1‑149 (MQ=255)
                                                                                            aGCGACGAAGTTGAAGTGCCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                                                      >  2:24147/1‑149 (MQ=255)
                                                                                            aGCGACGAAGTAGAAGTGACCTAATTACTTGTTAAAGTAGGCGATAAAGGTGAAGCTGAACAGTCGCTGATCACCTTTGTTGGCGACAAGGCCTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                                                      <  1:834060/149‑1 (MQ=255)
                                                                                              cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTgg                                                    <  2:541299/149‑1 (MQ=255)
                                                                                              cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTgg                                                    <  1:1016480/149‑1 (MQ=255)
                                                                                              cgacgaAGGTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGTACGTgg                                                    <  2:18617/149‑1 (MQ=255)
                                                                                               gacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGTTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                                   >  1:202768/1‑149 (MQ=255)
                                                                                               gacgaAGGTGAAGTGACCGAAATCCTGGTGACAGTTGGCGATACAGGTGAAGCTGAACAGTCGCTGAGCACCGTCGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                                   <  1:561428/149‑1 (MQ=255)
                                                                                                      ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACaaa                                            >  1:860623/1‑149 (MQ=255)
                                                                                                      ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACaaa                                            >  1:422359/1‑149 (MQ=255)
                                                                                                        gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt                                          <  1:423660/149‑1 (MQ=255)
                                                                                                        gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt                                          >  1:533497/1‑149 (MQ=255)
                                                                                                        gAAATGACCGATATCCTGGTGAAAGTGGGCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt                                          <  1:952297/149‑1 (MQ=255)
                                                                                                             caCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa                                                                       <  2:812303/114‑1 (MQ=14)
                                                                                                             caCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa                                                                       >  1:812303/2‑115 (MQ=14)
                                                                                                              aCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGGCGTGAAAGACCTGAAAGTCAACGTTGGAGAGAAAgtgaaaac                                    >  2:733102/1‑144 (MQ=11)
                                                                                                                cGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGTGAt                                                                  >  1:299478/1‑117 (MQ=14)
                                                                                                                cGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGTGAt                                                                  <  2:299478/117‑1 (MQ=14)
                                                                                                                cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAATTGAACGTGGGTGACAAAGTGTCTACCg                                  >  1:171324/1‑149 (MQ=255)
                                                                                                                 gAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg                                 >  1:212057/1‑149 (MQ=255)
                                                                                                                  aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                                                                               <  2:675540/70‑1 (MQ=255)
                                                                                                                  aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                                                                               >  1:675540/1‑70 (MQ=255)
                                                                                                                      ccTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGc                                                                                             <  1:592610/84‑1 (MQ=255)
                                                                                                                      catGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGc                                                                                             >  2:592610/3‑84 (MQ=255)
                                                                                                                          gTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCg                                                                                               <  2:446814/78‑1 (MQ=255)
                                                                                                                          gTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCg                                                                                               >  1:446814/1‑78 (MQ=255)
                                                                                                                           tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGAtt                       <  2:533497/149‑1 (MQ=255)
                                                                                                                              aaGTGGGCGATAACGTTGATGCTGTACAGTCGCAGATCTCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCTCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCGCGGTGATTATg                    <  1:811828/149‑1 (MQ=255)
                                                                                                                               aGTCGGCGATACCGTTGAAGCTGAACAGTCGCTGATCACCGTCGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGGGAACGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg                   <  2:144026/149‑1 (MQ=37)
                                                                                                                                gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGt                  <  1:348526/149‑1 (MQ=255)
                                                                                                                                      gacaaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAATGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAaga                                                                     <  1:903073/89‑1 (MQ=255)
                                                                                                                                      gacaaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAaga                                                                     >  2:903073/4‑92 (MQ=255)
                                                                                                                                       acaaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt                                                                                                           <  1:745022/51‑1 (MQ=255)
                                                                                                                                       acaaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt                                                                                                           >  2:745022/3‑53 (MQ=255)
                                                                                                                                         aaaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGt         >  2:168862/1‑149 (MQ=255)
                                                                                                                                         aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa                                                                                                     >  1:241583/1‑57 (MQ=255)
                                                                                                                                         aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa                                                                                                     <  2:241583/57‑1 (MQ=255)
                                                                                                                                            gTTGAAGCTGAACAGTCGCTGATCACCGTAGCAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgc      >  1:209317/1‑149 (MQ=255)
                                                                                                                                            gTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGGGACAAAGTGTCTACCGGCGCGCTGATTATGGTCTTCGAAGTcgc      <  2:755980/149‑1 (MQ=255)
                                                                                                                                             ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgcg     >  1:441582/1‑149 (MQ=255)
                                                                                                                                             ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATgg                                                                                                       >  1:945070/1‑51 (MQ=255)
                                                                                                                                             ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATgg                                                                                                       <  2:945070/51‑1 (MQ=255)
                                                                                                                                              tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGTCACCGTGAAAGAGATCAAAGTGAACGTGTGTGACAAAGTGTCTACCGGCTCGCTGATTATGGGCTTCGAAGTCGCgg    <  1:313347/149‑1 (MQ=255)
                                                                                                                                              tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCgg    <  1:13219/149‑1 (MQ=255)
                                                                                                                                              tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGGGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCgg    <  2:860623/149‑1 (MQ=255)
                                                                                                                                              ggAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCgg    <  2:344298/148‑1 (MQ=255)
                                                                                                                                                aaGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGCGTGACAAAGTGTCTACCGGCTCGCTGAATATGGTCTTCGAAGTCGCGGGt  >  1:79838/1‑149 (MQ=255)
                                                                                                                                                   cTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGtt                                                                                       >  2:413233/1‑61 (MQ=255)
                                                                                                                                                   cTGAACAGTCGCTGATCACCGTAGAAGGCGACAACGCTTCTATGGAAGTTCCGGCGCCGtt                                                                                       <  1:413233/61‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGT  >  NC_000913/125945‑126237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: