Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,321 C→A 8.3% noncoding (65/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,3210CA 8.3% 166.1 / 5.2 75noncoding (65/82 nt)omrBsRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base C (25/43);  new base A (3/3);  total (28/47)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

ATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976175‑2976467
                                                                                                                                                  |                                                                                                                                                  
atcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTg                                                                                                                                                  >  1:119232/1‑149 (MQ=255)
  cactACCTCTGGGATCTTGATTGTGGGCTGTACGACGACTATTCGCCTGTTAGAAAACGCAAAGGAATTAAGTTAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGTCCGGCAAAAAAAACCTGCGCATCTgcg                                                                                                                                                <  1:683657/146‑1 (MQ=255)
  caatAACTATGGCAGCTTGTTTTTGGTATGAACTTCGACTCTTCGCCAGCTAGAAAACGCAAACGAATGCAGGGAAATGCTACGATGTGTGTAAATTGTCGGTTACTGTTACAGATATATGACCGGCATAAATAACCTGCGCATCTgcg                                                                                                                                                <  2:568786/149‑1 (MQ=255)
   aatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATgcgc                                                                                                                                               <  1:753531/149‑1 (MQ=255)
    atACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCa                                                                                                                                              <  1:532388/149‑1 (MQ=255)
      aCCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTTTTACAGATTGAAGACCGGCAAAAAAAACCTGCGAATCTGCGCAgg                                                                                                                                            >  2:1022039/1‑149 (MQ=255)
         actGGGAACTTTATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAACGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTg                                                                                                                                         <  1:520944/148‑1 (MQ=255)
           tGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTTTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgt                                                                                                                                       >  2:903927/1‑149 (MQ=255)
           tGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttttt                                                                                                                                       <  2:1003438/149‑6 (MQ=255)
            gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGATGTGTGTAAATTTTGGTTTAATTTTAGTGTTTGATGACCGGCAAAAAAATCCTGCGCATCTGCGCAGGCTGgtg                                                                                                                                      <  2:104739/149‑1 (MQ=255)
             ggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCTCAGGCTGgtgt                                                                                                                                     >  2:680902/1‑149 (MQ=255)
                tCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCTAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAAt                                                                                                                                  >  1:409120/1‑149 (MQ=255)
                tCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAAt                                                                                                                                  <  2:1017428/149‑1 (MQ=255)
                  ttGATTGTGGGCTGCACGACGACTCTTCGCCAGCGAGAAACCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTc                                                                                                                                <  1:180960/149‑1 (MQ=255)
                    taTTGTGGTCTGCACGACGACTCTTCGCCATCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAt                                                                                                                              <  1:60559/148‑1 (MQ=255)
                    gtttGTGGTCTGCACGACGACTCTACGCCAGCTAGAAAACTCATAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGCCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAt                                                                                                                              <  2:694794/147‑1 (MQ=255)
                     aTTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGAATGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtg                                                                                                                             <  1:887196/149‑1 (MQ=255)
                       tgtgGTCTGCACTACGACTCTTCGCCTGGCTGAAAACTGAAATGAATGAAGGGAAATGCTACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                           <  1:485131/149‑1 (MQ=255)
                       tgtgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                           <  2:506015/149‑1 (MQ=255)
                        gtgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGc                                                                                                                          <  2:756456/149‑1 (MQ=255)
                         tgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCt                                                                                                                         >  1:980209/1‑149 (MQ=255)
                         tgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCt                                                                                                                         >  1:976838/1‑149 (MQ=255)
                            tCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCaa                                                                                                                      >  2:898684/1‑149 (MQ=255)
                                  cgacgaCTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGaa                                                                                                                <  2:186030/149‑1 (MQ=255)
                                     cgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGtt                                                                                                             <  1:638531/149‑1 (MQ=255)
                                      gacTCTTCGACAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTg                                                                                                            <  1:883436/149‑1 (MQ=255)
                                           ttCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTc                                                                                                       <  1:974965/149‑1 (MQ=255)
                                            tCGCCAGCGAGAAAACGCAAAGGAATGACGGGAAATGCAACGAGGGGTGGAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCACAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCa                                                                                                      <  2:788036/149‑1 (MQ=255)
                                             cGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAc                                                                                                     <  1:391000/149‑1 (MQ=255)
                                               ccAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCt                                                                                                   >  2:509564/1‑149 (MQ=255)
                                                 aGCGAGAAAACGCAAAGGAATGAAGGGAAATGCTACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTAt                                                                                                 <  1:58833/149‑1 (MQ=255)
                                                       aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGATGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAACTATCAATAc                                                                                           >  2:260531/1‑149 (MQ=255)
                                                             catcggAATGAATGGAAATGCAACGATGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCACCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTgg                                                                                     <  2:787662/145‑1 (MQ=255)
                                                              aaaGGAATGAAGTGAAATGCAACGAGGTGTGTAAAATGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTTGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    <  1:339032/149‑1 (MQ=255)
                                                              aaaGGAATGAAGGGAAATTCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGTCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    <  2:435324/149‑1 (MQ=255)
                                                              aaaGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    >  1:1925/1‑149 (MQ=255)
                                                              aaaGGAATGAAGGGAAATGCAACGAGGTGTATAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    <  1:315363/149‑1 (MQ=255)
                                                                    atcaaGCGAAATCCAACGAGTGGTGTACATTGTCGGTGACTGTTAAAGATTGATGAACGCCAAAAAAAACCTGCGCATCTGCGCAGGCTCGAGTAATTCATGTGCTCCACCAGAATTTGACTTCACCTATCAATACCTCTGGGATcacc                                                                              <  1:657896/146‑1 (MQ=255)
                                                                          ggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGCTGACCGGCAAAAAACACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTa                                                                        <  1:462110/149‑1 (MQ=255)
                                                                            aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAACCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCCACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                                                                      >  2:732298/1‑149 (MQ=255)
                                                                            aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGTTGTAATTCATGTGCTCAACCAGAAGTTGACTTCACCTATCAATACCTCTGGGATAACCACTTTAGc                                                                      >  1:610121/1‑149 (MQ=255)
                                                                            aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                                                                      >  1:264153/1‑149 (MQ=255)
                                                                            aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                                                                      >  1:39249/1‑149 (MQ=255)
                                                                                   aCGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAATAAATCCTGCGCATCTGCGCAGGCTGGGGTAATTCCTGTGCTCAAACCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTTGCAACCTGa                                                               >  1:913555/1‑149 (MQ=255)
                                                                                    ctaGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCCGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGaa                                                              <  1:838770/147‑1 (MQ=255)
                                                                                        gtgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCa                                                          >  2:342298/1‑149 (MQ=255)
                                                                                                atttgcGGCTACTTTTCCAGATAGATTCCCGGCTATTAAAAGCTGCGCTTCTGCTCATGCTTGTGTAATTCATGTTCGCAAGTAGATTTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGccac                                                  <  2:989383/144‑1 (MQ=255)
                                                                                                 ttGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGccacc                                                 >  1:548339/1‑149 (MQ=255)
                                                                                                    tCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCGCCACCCGAAGTTGCCTTCACCTATCACTACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGc                                              >  2:776421/1‑149 (MQ=255)
                                                                                                     cGGTTTCTGTTACAGATTGTTGACCTGATTAAATAACCTGCGAATCTGCGAATGCTGGTGTAATTGATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAGCTCTGGGATCACCACTTTAGCAACCTGAATCCAAACGCCACCAGCg                                             <  1:109868/149‑1 (MQ=255)
                                                                                                     cGGGTACTTTTCCAGATTGATGACCGGCAAAAAAAACCTGCGCGTCTGAGCAGGCTGGTGAAATTCTTGTGCTCAACCAGATTTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCg                                             <  2:772137/149‑1 (MQ=255)
                                                                                                       gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGACCACCACTTTAGCCACCTGAAGCCAAACGCCACCCGCGGt                                           >  2:351039/1‑149 (MQ=255)
                                                                                                       gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGAAAACCACATAAGCCACCTGAAGCCAAACGCCACCAGCGGt                                           >  2:255447/1‑149 (MQ=255)
                                                                                                             gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCg                                     <  1:826974/149‑1 (MQ=255)
                                                                                                              ttACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAACCGCCACCAGCGGt                                           >  2:694196/1‑142 (MQ=255)
                                                                                                              ttACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                           <  1:694196/142‑1 (MQ=255)
                                                                                                              ttACAGATAGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCCTGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGc                                    <  1:649628/149‑1 (MQ=255)
                                                                                                                ataGATTTCTGACCGGCATAAATTTCCTGCGCTTTTGCTGAGGCTGGTGTAATTGATGTTCTCAACCAGATGTTGACTTCACCTATCAATACCTCTGGGATCACCTCTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCaa                                  <  2:594081/147‑1 (MQ=255)
                                                                                                                aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCaa                                  >  1:324334/1‑149 (MQ=255)
                                                                                                                 cAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCGCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAAc                                 <  1:9979/149‑1 (MQ=255)
                                                                                                                         tgaACGCCAAAAACAACCTGCGCATAGGCGCAGGCTGGTGTAATTCATGTGATCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTc                         <  2:956622/149‑1 (MQ=255)
                                                                                                                              ggaaaaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGAGCTCAACCAGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCaaa                    <  1:64548/146‑1 (MQ=255)
                                                                                                                              ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCaaa                    <  1:903927/149‑1 (MQ=255)
                                                                                                                               gCAAAAAAAACCTTCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAg                   <  1:1022039/149‑1 (MQ=255)
                                                                                                                               gCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAg                   >  1:190052/1‑149 (MQ=255)
                                                                                                                                    ataaaCCTGCGCATCTGCGCTGGCTGGTGTAATTCATGTGCTCTACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTaa              <  2:326357/147‑1 (MQ=255)
                                                                                                                                    aaaaaCCTGCGCTTCTGAGCTTGCTGGTGTAGTTCATGTGCTCAACCCGAATTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTaa              <  1:746402/149‑1 (MQ=255)
                                                                                                                                    aaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCTCCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCATCGGTCAATCGCAACAGCGTTTCGCAAAGTGTaa              <  1:680902/149‑1 (MQ=255)
                                                                                                                                      cagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGCCTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc            >  1:524687/4‑149 (MQ=255)
                                                                                                                                      cagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc            >  1:608570/4‑149 (MQ=255)
                                                                                                                                      cagccTGCGCAGATGCGCAGGCTGGTGTAATTCAAGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc            >  1:341806/4‑149 (MQ=255)
                                                                                                                                            gcgcATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACATCACCTATCAATACCTCTGGGATCACCACTTTAGCAAACTGAAGCCAAACGACACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGt      >  2:563406/1‑149 (MQ=255)
                                                                                                                                             cgcATCTGCGCAGGCTTGTGTAATTCATGTGCTCAACCCGAAGTTTACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGtt     <  2:942928/149‑1 (MQ=255)
                                                                                                                                              gcATCTGCGCAGGCTTGTGAAATTCATCTGCTAAACCCGATCTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGACACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGttt    <  1:844083/149‑1 (MQ=255)
                                                                                                                                                aTCAGCGCTGGCTGGTGGAATTCATGTGCTCATCCCGACGTTGACCTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGc  <  1:646864/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976175‑2976467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑