Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,852 C→T 6.3% intergenic (+19/+27) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8520CT 6.3% 211.6 / 2.3 79intergenic (+19/+27)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base T (2/3);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGAT  >  NC_000913/3171704‑3171998
                                                                                                                                                    |                                                                                                                                                  
aCCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGc                                                                                                                                                    >  1:525201/1‑149 (MQ=255)
  ccGGACAAACCGCTACCGGCAGCGGGCATGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGCAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCg                                                                                                                                                  >  2:897927/1‑149 (MQ=255)
  ccGGACAAACCGATACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGATCAAGGTGGATTTGAGGCGAAGGTAAGCCCGTAACTTCTCTGTCTATGAGAGCCg                                                                                                                                                  >  1:52728/1‑149 (MQ=255)
  ccGCACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAAACGCCAAATTGCATGGCAAGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCg                                                                                                                                                  <  1:205192/149‑1 (MQ=255)
   cGGACAAACCGCTACCGGCAGCCGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGt                                                                                                                                                 <  2:244999/149‑1 (MQ=255)
        aaaCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTTACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                                                            >  1:218531/1‑149 (MQ=255)
        aaaCCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                                                            <  1:438310/149‑1 (MQ=255)
           ccGCTACCGGCAGCGGGCGTGGGCTATCGATTGTCCAGCGACTCGCCAAATTGCAGGGCATGAATGTTGAATTTGGTCTTGCGGAACAACGGGGAGTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCAGTTAACACgc                                                                                                                                         >  1:676168/1‑148 (MQ=255)
            cGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAATGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                                                                        <  1:181242/149‑1 (MQ=255)
            cGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGAGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTTGATGTGAGGCGAAGGTAAGCACGTAACTTCTCTGTCTATGAGAGCCGTTAAATCgcc                                                                                                                                        >  2:698107/1‑147 (MQ=255)
              gtACCGGCTGCGGGGTTGGGCTTTGGATTGTCCTGCGAATCGCCATATTGGTTGGCTTGAATGTTGAATTTGGGAATGCGGAACATGGTGGATTTGTGGCGAAGGTATGCTGGTAAATTCTCTGTCTATGAGAGCCGTTAAAACGActc                                                                                                                                      <  2:437921/148‑1 (MQ=255)
               tACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGATTCGCCATATTGCATGGCATGTATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGctct                                                                                                                                     <  2:523103/149‑1 (MQ=255)
                  cGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                  <  2:430697/149‑1 (MQ=255)
                  cGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                  <  1:272708/149‑1 (MQ=255)
                     cAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGATTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                                                               >  1:481352/1‑149 (MQ=255)
                        cGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCTGGGCAGGAATGTTGAATTTGGGACTGCGGACCAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTACAACGACTCTCATAGAttt                                                                                                                            <  1:722683/149‑1 (MQ=255)
                          ggCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATTTTGAATTTGGGAATGCTGAACAAGGTGGATTTTAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                                                                          <  2:10565/149‑1 (MQ=255)
                           gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                                                         <  2:17317/149‑1 (MQ=255)
                           gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGAGTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                                                         <  1:558810/149‑1 (MQ=255)
                            cTTGGGCTATCGATTGTCCAGCGATTCGCCAAATTGCATGCCATGAATGTTGAATTTGTGAATGCGGAACATGGTGTATTTGAGGCGTAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                                                                        <  2:576071/149‑1 (MQ=255)
                               gggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                     <  1:238886/149‑1 (MQ=255)
                                ggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGGGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                                                                    <  1:203202/149‑1 (MQ=255)
                                ggCTATCGACTGTCCAGCGAATCCCCAAATTGCATGGCATGAATGTTGAACTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                                                                    <  2:340194/149‑1 (MQ=255)
                                            tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                                                                        <  1:750739/149‑1 (MQ=255)
                                                 cGAATAGCCAAATTTCATGGCATGAATGTTTAATTTGGGAATGCGGAACAAGTTTGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTc                                                                                                   <  1:64886/149‑1 (MQ=255)
                                                    aTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                                                                <  2:525201/149‑1 (MQ=255)
                                                     tCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcacaa                                                                                               >  2:261880/1‑145 (MQ=255)
                                                     tCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGAGTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCa                                                                                               >  1:558351/1‑149 (MQ=255)
                                                          aactctcatggcctgAATGTTTAATTTGCGAATCCGTAACAAGGTGGCTTTGAGGCGAATGTCAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                          <  2:951360/143‑1 (MQ=255)
                                                            aTTGCATGGCTTGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTGTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc                                                                                        <  2:418125/149‑1 (MQ=255)
                                                            aTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc                                                                                        <  2:771914/149‑1 (MQ=255)
                                                                catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATATGAGGCGAAGGTAAGCTGGAAACATCTCTGTCTATGAGAGACGTTAAAACGACTCTCATATATTTTAATAATAGCAAAATAAAAAAATACCCCAAAAAACccacc                                                                                    >  2:585195/1‑149 (MQ=255)
                                                                 atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAACCGACTCACATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAACAGacacca                                                                                   >  2:572253/1‑149 (MQ=255)
                                                                 atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGacacca                                                                                   >  1:917733/1‑149 (MQ=255)
                                                                   ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACACTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                                                                 >  1:362711/1‑149 (MQ=255)
                                                                      atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                              >  1:287343/1‑149 (MQ=255)
                                                                             ttGAATTTGGGAATGCGGAACACGGTGGATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCACAAAAAAGCCAccaaccaccaaccac                                                                       >  2:773531/1‑148 (MQ=255)
                                                                              tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                                                                      <  1:438356/149‑1 (MQ=255)
                                                                              tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTAAAAACGACTCTCATAGATTTTACTAATATCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAgc                                                                      >  2:216856/1‑148 (MQ=255)
                                                                              tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGGCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                                                                      <  1:770230/149‑1 (MQ=255)
                                                                               gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                                                                     <  1:89081/149‑1 (MQ=255)
                                                                               gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCCTAGATTTTACTAATAGCAATATATAAGCCGTCCCCAAAAAAGCCGCCAACCACAAACCAGAc                                                                     >  2:469734/1‑149 (MQ=255)
                                                                                aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCAAAAAAAGCCACCAACCACAAACCAGACa                                                                    >  1:575920/1‑149 (MQ=255)
                                                                                 aTTTGGGAATTCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATAAAAACCGTCCCCATAAAAGCCCCCAACCACAAACCAGACaa                                                                   >  2:482221/1‑149 (MQ=255)
                                                                                       gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                             <  2:833949/149‑1 (MQ=255)
                                                                                         aTGCTGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                           <  2:212092/149‑1 (MQ=255)
                                                                                           gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                         >  1:761801/1‑149 (MQ=255)
                                                                                            cGGAACAAGGTGGATTTGAGTCGAATGTAAGCTGGTAACTTCTCTGTCTATGATAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCTCCAACCACAAACCAGACAAATACcgagcga                                                        <  1:874527/149‑1 (MQ=255)
                                                                                              gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga                                                      >  1:458923/1‑149 (MQ=255)
                                                                                                    ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACat                                                <  1:19426/149‑1 (MQ=255)
                                                                                                    ggTGGATTGGAGGTGAAGGTTATCTTGTAATTTCTCGTGATTTGAGAGCCGTTAAAAAGACTTTCATAGTTTTTACTAATATACATATATAAAACGTCCCCATCAACGCCACCAACCACAAACCAGACAAATACCGCGCGAGAATAcct                                                <  2:676168/149‑3 (MQ=255)
                                                                                                     gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACACACCAGACAAATACCGAGCGAGAATACata                                               >  2:826525/1‑149 (MQ=255)
                                                                                                        gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGACGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAc                                            <  2:761483/149‑1 (MQ=255)
                                                                                                         aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACTACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAcc                                           <  1:504916/149‑1 (MQ=255)
                                                                                                         aTTTGAGGCGAAGGTAACCTGCAAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCACAAAGATTTTACTAAAAGCAAAATATAACCCGTCCCCAAAAAAGCCACCAACCCCAAACCCGCCAAATGCCGAGCGCGAATACAGATccc                                           >  2:699389/1‑149 (MQ=255)
                                                                                                               ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAAc                                     >  1:350900/1‑149 (MQ=255)
                                                                                                                gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                    >  2:477877/1‑149 (MQ=255)
                                                                                                                gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCACATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCATCCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                    >  1:91777/1‑149 (MQ=255)
                                                                                                                  gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTATTAGCAAAATATAAACCGTCCCCAAAAAAGCCACAAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCg                                  >  1:34132/1‑149 (MQ=255)
                                                                                                                  gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCg                                  <  1:897927/149‑1 (MQ=255)
                                                                                                                     ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAACCCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                               >  1:642668/1‑149 (MQ=255)
                                                                                                                     ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                               <  2:860657/149‑1 (MQ=255)
                                                                                                                      gTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAGACCGTCCCCAAAAAAGCCACCAACCACAAACCACACAAATACCGCGCGAGAATACATATAACCAAAACTCGAACa                              >  2:921050/1‑149 (MQ=255)
                                                                                                                        aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGAGAGAATACATATACCCAAAACTCGAACAtt                            >  1:126578/1‑149 (MQ=255)
                                                                                                                                aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCAt                    >  1:279280/1‑149 (MQ=255)
                                                                                                                                   ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAATCCGTCCCCAAACAAGCCACCAACCACACACCAGCCAAATACCGCGCGAGAATACATCTACCCAAAACTCGAACAGTTCCCGCATaaa                 >  2:790230/1‑149 (MQ=255)
                                                                                                                                   ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCCCCATCCACAAAGCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAGTTCCCGCATaag                 >  1:54201/1‑148 (MQ=255)
                                                                                                                                   ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa                 >  2:551152/1‑149 (MQ=255)
                                                                                                                                     ctctGTCTATGATATCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAACGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga               <  2:143725/149‑1 (MQ=255)
                                                                                                                                      tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag              <  1:976602/149‑1 (MQ=255)
                                                                                                                                          tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt          <  2:939540/149‑1 (MQ=255)
                                                                                                                                             aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt       <  2:390091/149‑1 (MQ=255)
                                                                                                                                              tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGCACATTTCCCGCATAAAGAGTTTTCTta      >  1:469263/1‑149 (MQ=255)
                                                                                                                                              tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAACAGACAAAAACCGAGAGAGAATACATATACCCAAAACTCGAACATTAAACGAATAAAGAGTTTTCTta      >  2:790707/1‑149 (MQ=255)
                                                                                                                                               gagaGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa     >  1:138497/1‑149 (MQ=255)
                                                                                                                                                agagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAAATATACCCAAAACTCGAACATTACCCGCATAAAGCGTTTTCTtaag    >  1:169240/1‑149 (MQ=255)
                                                                                                                                                agagCCGGTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCACACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaag    <  2:558351/149‑1 (MQ=255)
                                                                                                                                                 gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGGAAAATATAAACCGTCCCCAAAAAAGCCACCACCCACAAACCAGACAAATACCGAGCGAGAATACATATCACCAAAACTCGAACATTTCCCGCATAAAGCGTTTTCTtaag  >  2:672554/1‑148 (MQ=255)
                                                                                                                                                 gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAACCTCGAACATTTCCCGCATAAAGAGTTTTCTtaaga   <  2:176993/149‑1 (MQ=255)
                                                                                                                                                  agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat  <  2:75160/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGAT  >  NC_000913/3171704‑3171998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: