Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,863 A→G 6.4% intergenic (+30/+16) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8630AG 6.4% 227.9 / 5.2 78intergenic (+30/+16)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (37/36);  new base G (2/3);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

CGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAAT  >  NC_000913/3171716‑3172007
                                                                                                                                                   |                                                                                                                                                
cGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAATGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                                                                                 <  1:181242/149‑1 (MQ=255)
  gtACCGGCTGCGGGGTTGGGCTTTGGATTGTCCTGCGAATCGCCATATTGGTTGGCTTGAATGTTGAATTTGGGAATGCGGAACATGGTGGATTTGTGGCGAAGGTATGCTGGTAAATTCTCTGTCTATGAGAGCCGTTAAAACGActc                                                                                                                                               <  2:437921/148‑1 (MQ=255)
   tACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGATTCGCCATATTGCATGGCATGTATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGctct                                                                                                                                              <  2:523103/149‑1 (MQ=255)
      cGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                           <  2:430697/149‑1 (MQ=255)
      cGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                                                                           <  1:272708/149‑1 (MQ=255)
         cAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGATTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGa                                                                                                                                        >  1:481352/1‑149 (MQ=255)
            cGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCTGGGCAGGAATGTTGAATTTGGGACTGCGGACCAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTACAACGACTCTCATAGAttt                                                                                                                                     <  1:722683/149‑1 (MQ=255)
              ggCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATTTTGAATTTGGGAATGCTGAACAAGGTGGATTTTAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa                                                                                                                                   <  2:10565/149‑1 (MQ=255)
               gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                                                                  <  2:17317/149‑1 (MQ=255)
               gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGAGTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                                                                  <  1:558810/149‑1 (MQ=255)
                cTTGGGCTATCGATTGTCCAGCGATTCGCCAAATTGCATGCCATGAATGTTGAATTTGTGAATGCGGAACATGGTGTATTTGAGGCGTAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                                                                                 <  2:576071/149‑1 (MQ=255)
                   gggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAt                                                                                                                              <  1:238886/149‑1 (MQ=255)
                    ggCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGGGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                                                                             <  1:203202/149‑1 (MQ=255)
                    ggCTATCGACTGTCCAGCGAATCCCCAAATTGCATGGCATGAATGTTGAACTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                                                                             <  2:340194/149‑1 (MQ=255)
                                tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                                                                                 <  1:750739/149‑1 (MQ=255)
                                     cGAATAGCCAAATTTCATGGCATGAATGTTTAATTTGGGAATGCGGAACAAGTTTGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTc                                                                                                            <  1:64886/149‑1 (MQ=255)
                                        aTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcccc                                                                                                         <  2:525201/149‑1 (MQ=255)
                                         tCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcacaa                                                                                                        >  2:261880/1‑145 (MQ=255)
                                         tCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGAGTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCa                                                                                                        >  1:558351/1‑149 (MQ=255)
                                              aactctcatggcctgAATGTTTAATTTGCGAATCCGTAACAAGGTGGCTTTGAGGCGAATGTCAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                   <  2:951360/143‑1 (MQ=255)
                                                aTTGCATGGCTTGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTGTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc                                                                                                 <  2:418125/149‑1 (MQ=255)
                                                aTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc                                                                                                 <  2:771914/149‑1 (MQ=255)
                                                    catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATATGAGGCGAAGGTAAGCTGGAAACATCTCTGTCTATGAGAGACGTTAAAACGACTCTCATATATTTTAATAATAGCAAAATAAAAAAATACCCCAAAAAACccacc                                                                                             >  2:585195/1‑149 (MQ=255)
                                                     atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAACCGACTCACATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAACAGacacca                                                                                            >  2:572253/1‑149 (MQ=255)
                                                     atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGacacca                                                                                            >  1:917733/1‑149 (MQ=255)
                                                       ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACACTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                                                                          >  1:362711/1‑149 (MQ=255)
                                                          atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                       >  1:287343/1‑149 (MQ=255)
                                                                 ttGAATTTGGGAATGCGGAACACGGTGGATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCACAAAAAAGCCAccaaccaccaaccac                                                                                >  2:773531/1‑148 (MQ=255)
                                                                  tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                                                                               <  1:438356/149‑1 (MQ=255)
                                                                  tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTAAAAACGACTCTCATAGATTTTACTAATATCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAgc                                                                               >  2:216856/1‑148 (MQ=255)
                                                                  tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGGCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                                                                               <  1:770230/149‑1 (MQ=255)
                                                                   gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                                                                              <  1:89081/149‑1 (MQ=255)
                                                                   gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCCTAGATTTTACTAATAGCAATATATAAGCCGTCCCCAAAAAAGCCGCCAACCACAAACCAGAc                                                                              >  2:469734/1‑149 (MQ=255)
                                                                    aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCAAAAAAAGCCACCAACCACAAACCAGACa                                                                             >  1:575920/1‑149 (MQ=255)
                                                                     aTTTGGGAATTCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATAAAAACCGTCCCCATAAAAGCCCCCAACCACAAACCAGACaa                                                                            >  2:482221/1‑149 (MQ=255)
                                                                           gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                                      <  2:833949/149‑1 (MQ=255)
                                                                             aTGCTGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                    <  2:212092/149‑1 (MQ=255)
                                                                               gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                                  >  1:761801/1‑149 (MQ=255)
                                                                                cGGAACAAGGTGGATTTGAGTCGAATGTAAGCTGGTAACTTCTCTGTCTATGATAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCTCCAACCACAAACCAGACAAATACcgagcga                                                                 <  1:874527/149‑1 (MQ=255)
                                                                                  gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga                                                               >  1:458923/1‑149 (MQ=255)
                                                                                        ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACat                                                         <  1:19426/149‑1 (MQ=255)
                                                                                        ggTGGATTGGAGGTGAAGGTTATCTTGTAATTTCTCGTGATTTGAGAGCCGTTAAAAAGACTTTCATAGTTTTTACTAATATACATATATAAAACGTCCCCATCAACGCCACCAACCACAAACCAGACAAATACCGCGCGAGAATAcct                                                         <  2:676168/149‑3 (MQ=255)
                                                                                         gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACACACCAGACAAATACCGAGCGAGAATACata                                                        >  2:826525/1‑149 (MQ=255)
                                                                                            gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGACGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAc                                                     <  2:761483/149‑1 (MQ=255)
                                                                                             aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACTACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAcc                                                    <  1:504916/149‑1 (MQ=255)
                                                                                             aTTTGAGGCGAAGGTAACCTGCAAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCACAAAGATTTTACTAAAAGCAAAATATAACCCGTCCCCAAAAAAGCCACCAACCCCAAACCCGCCAAATGCCGAGCGCGAATACAGATccc                                                    >  2:699389/1‑149 (MQ=255)
                                                                                                   ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAAc                                              >  1:350900/1‑149 (MQ=255)
                                                                                                    gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                             >  2:477877/1‑149 (MQ=255)
                                                                                                    gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCACATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCATCCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                             >  1:91777/1‑149 (MQ=255)
                                                                                                      gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTATTAGCAAAATATAAACCGTCCCCAAAAAAGCCACAAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCg                                           >  1:34132/1‑149 (MQ=255)
                                                                                                      gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCg                                           <  1:897927/149‑1 (MQ=255)
                                                                                                         ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAACCCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                        >  1:642668/1‑149 (MQ=255)
                                                                                                         ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                        <  2:860657/149‑1 (MQ=255)
                                                                                                          gTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAGACCGTCCCCAAAAAAGCCACCAACCACAAACCACACAAATACCGCGCGAGAATACATATAACCAAAACTCGAACa                                       >  2:921050/1‑149 (MQ=255)
                                                                                                            aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGAGAGAATACATATACCCAAAACTCGAACAtt                                     >  1:126578/1‑149 (MQ=255)
                                                                                                                    aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCAt                             >  1:279280/1‑149 (MQ=255)
                                                                                                                       ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAATCCGTCCCCAAACAAGCCACCAACCACACACCAGCCAAATACCGCGCGAGAATACATCTACCCAAAACTCGAACAGTTCCCGCATaaa                          >  2:790230/1‑149 (MQ=255)
                                                                                                                       ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCCCCATCCACAAAGCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAGTTCCCGCATaag                          >  1:54201/1‑148 (MQ=255)
                                                                                                                       ttCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATaaa                          >  2:551152/1‑149 (MQ=255)
                                                                                                                         ctctGTCTATGATATCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAACGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAaga                        <  2:143725/149‑1 (MQ=255)
                                                                                                                          tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag                       <  1:976602/149‑1 (MQ=255)
                                                                                                                              tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGtttt                   <  2:939540/149‑1 (MQ=255)
                                                                                                                                 aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                <  2:390091/149‑1 (MQ=255)
                                                                                                                                  tGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGCACATTTCCCGCATAAAGAGTTTTCTta               >  1:469263/1‑149 (MQ=255)
                                                                                                                                  tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAACAGACAAAAACCGAGAGAGAATACATATACCCAAAACTCGAACATTAAACGAATAAAGAGTTTTCTta               >  2:790707/1‑149 (MQ=255)
                                                                                                                                   gagaGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa              >  1:138497/1‑149 (MQ=255)
                                                                                                                                    agagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAAATATACCCAAAACTCGAACATTACCCGCATAAAGCGTTTTCTtaag             >  1:169240/1‑149 (MQ=255)
                                                                                                                                    agagCCGGTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCACACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaag             <  2:558351/149‑1 (MQ=255)
                                                                                                                                     gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGGAAAATATAAACCGTCCCCAAAAAAGCCACCACCCACAAACCAGACAAATACCGAGCGAGAATACATATCACCAAAACTCGAACATTTCCCGCATAAAGCGTTTTCTtaagt            >  2:672554/1‑148 (MQ=255)
                                                                                                                                     gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAACCTCGAACATTTCCCGCATAAAGAGTTTTCTtaaga            <  2:176993/149‑1 (MQ=255)
                                                                                                                                      agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat           <  2:75160/149‑1 (MQ=255)
                                                                                                                                         cGTTAAAACGACTCTCATAGATTTTACTAAAAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataag        <  2:215477/149‑1 (MQ=255)
                                                                                                                                          gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga       >  1:453812/1‑149 (MQ=255)
                                                                                                                                           ttAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTAAAGATAAGaa      >  2:457337/1‑149 (MQ=255)
                                                                                                                                           ttAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGATTTCTTAAGATAAGaa      >  2:20103/1‑149 (MQ=255)
                                                                                                                                             aaaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaata    >  2:32628/1‑149 (MQ=255)
                                                                                                                                               aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat  >  2:246953/1‑149 (MQ=255)
                                                                                                                                               aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACAGATCTAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTGAAGATAAGaataat  >  1:591443/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGCTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAAT  >  NC_000913/3171716‑3172007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: