| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,194,875 | T→C | 11.8% | intergenic (‑10/+224) | sibD ← / ← sibE | sRNA antisense regulator of toxic IbsD protein/sRNA antisense regulator of toxic IbsE protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,194,875 | 0 | T | C | 11.8% | 125.0 / 8.9 | 54 | intergenic (‑10/+224) | sibD/sibE | sRNA antisense regulator of toxic IbsD protein/sRNA antisense regulator of toxic IbsE protein |
| Reads supporting (aligned to +/- strand): ref base T (20/25); new base C (3/3); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01 | |||||||||||
CCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCA > NC_000913/3194728‑3195022 | cccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttacttt < 1:98855/149‑1 (MQ=255)cccTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctataattt > 1:208608/1‑149 (MQ=255) ccTCCCGGAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttacttta < 2:754969/149‑1 (MQ=255) cccGTAGAAGGGGGCCTTTCTGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGt < 1:458870/149‑1 (MQ=255) cccGAAGAAGTGGGCCTTACAGAAGGAAAGGGTTATGAAGAAGCTCGTAATCATACTGATTGTGTTGTTACTCGTAAGTTTCTCAGCTTATTAACAGACAATCAGAGGGGGGAGAAATCCTCCCTCCCACTTGTTCCATTACTTTAgtt > 2:964491/1‑147 (MQ=18) ggggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaaa < 2:838392/149‑1 (MQ=255) ggggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaaa < 1:853505/149‑1 (MQ=255) ggCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATc > 2:107630/1‑149 (MQ=255) gCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCa > 2:988746/1‑149 (MQ=255) gCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCa < 2:621879/149‑1 (MQ=255) gCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCa > 2:591559/1‑149 (MQ=255) gCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGGTGTTACTCGTAAGGTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCGCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCa < 2:42074/149‑1 (MQ=255) cctACAGCCGGAAAGGGTTAGTCTGAACCTCGTCATCATACTGATTGTGGAGTGACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAACTCCTCCCGCACCCTTGTGCCTTTACTTTAGGTTGAAAAAAAATCAGc < 2:686079/147‑1 (MQ=255) aaGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTGTAGGTTGGAAAAAAATCAGCGTCaaga > 2:968811/1‑149 (MQ=255) gAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCTTAAGTTTCGCTTCTTATTAACAACAAATCAGAGGGGGGAGAAATCCTCCCTAAACCTTGTTCATTTACTATAGGTTGAAAAAACAACAGCGTCaataagc > 1:601617/1‑148 (MQ=11) aaGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTAACCATATTTCCTGTACATAAGTATGAAAAAAACTCAGCGGCAAGAAGCtt > 1:397387/1‑149 (MQ=18) gTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCAt > 1:392938/1‑149 (MQ=255) gatgatGGTCGTCATACTACTGTTTGTTTTGTTACTCGTAAGTTTCGCTGCTTATTAACAGCGAATAAGAGGGGGGAGACATCCTCCCGCACGCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGTTGGTTTCATTAaca < 2:122593/149‑1 (MQ=255) aGCTCGTCATCATACTGATTGTGTTGTTACTCGTTAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTACCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACatat < 2:736762/149‑1 (MQ=255) cTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATcc > 2:219098/1‑149 (MQ=255) tcatcaTACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTgg > 2:360458/1‑149 (MQ=255) catcatACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGc < 2:354706/149‑1 (MQ=255) catACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCa > 1:411497/1‑149 (MQ=255) aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGcc > 2:842250/1‑149 (MQ=255) aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCACCCTCACCCTTGTTCATTTAATATAGGTTGAAAAAAAAACAGCGACAAGAAGGATTCATTAAAACATATCCCTGACACAAAAAGcc > 1:657609/1‑149 (MQ=255) ttGGGTAGTTACTCGTAAGATTCGCAGCTTATTAACAGCCAATCAGAGGGGGTAGAAATCCTCCAGCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCATCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCg < 2:684394/149‑1 (MQ=255) ttACTCGTAAGTTTCGCAGCTAATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGAtttt < 1:501392/149‑1 (MQ=255) cTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTta < 1:591559/149‑1 (MQ=255) gTTTCGCAGCTTATTAACAGCCTATGAGATGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGc < 2:929660/149‑1 (MQ=255) gTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGc < 1:253891/149‑1 (MQ=255) tttCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg > 1:788212/1‑149 (MQ=255) cGCAGCTTATTAACATCCATTCAGAGGGGGGATAAATCATCCCTCACGCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGACCCAAATAGCCGGGGATTTTTTATATCTGCGTTc < 2:657609/149‑1 (MQ=39) aGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc < 1:286102/149‑1 (MQ=255) cTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa > 2:374472/1‑149 (MQ=255) ttattaACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGaa < 2:610587/66‑1 (MQ=255) ttattaACAGCCAATCAGAGGGGGGAGAAATCCTCCCTAACCCTTGTTCCTTTACTCTAGGTTGaa > 1:610587/1‑66 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAgg < 2:754186/56‑1 (MQ=11) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGt < 2:885687/57‑1 (MQ=11) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTGACCCTTGTTCCTTTACTCTAgg > 1:754186/1‑56 (MQ=14) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTCTAGGt > 1:885687/1‑57 (MQ=255) cAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGc < 1:964491/149‑1 (MQ=255) aGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCa > 1:63333/1‑149 (MQ=255) gCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCaa > 1:67978/1‑149 (MQ=255) cAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAAt > 2:958980/1‑149 (MQ=255) tCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGcg > 1:290872/1‑148 (MQ=255) tagaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTg < 2:891590/148‑1 (MQ=255) gagaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGTTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCTTTCCGCTAAAAGGTGAAAATGCTCAGGCCGTTg > 2:240426/1‑149 (MQ=255) taaaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCa < 1:432486/148‑1 (MQ=255) gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCa < 1:438900/149‑1 (MQ=255) gaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCa < 2:739122/149‑1 (MQ=255) tcctccCTCACCCTTGTTCCTATACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGAGTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGt > 1:780230/1‑149 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGtt > 1:653079/1‑149 (MQ=255) ctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGttt < 2:67978/149‑1 (MQ=255) tccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGtttt < 2:411497/149‑1 (MQ=255) ctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCg < 1:89815/149‑1 (MQ=255) gcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGt < 2:1015175/148‑1 (MQ=255) ttGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACTTTGCAGCGTTTTGCGTGACCGc > 1:474821/1‑149 (MQ=255) ctttactttaGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCgggg > 1:565672/1‑149 (MQ=255) ctttactttaGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCGGCGTTCCGCTAAAAGGTGCAAATGCTGAGGACGTTGCAGCGTTTTGCGTGACCGCTCgggg > 2:901267/1‑149 (MQ=255) ttactttaGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGaa < 1:360458/149‑1 (MQ=255) ctttaGGTTGAAAAAAAATCAGCGTCTAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCGAGGGGAAGGc > 1:416925/1‑149 (MQ=255) tttaGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCa < 2:392938/149‑1 (MQ=255) | CCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGCTCAGGACGTTGCAGCGTTTTGCGTGACCGCTCGGGGAAGGCA > NC_000913/3194728‑3195022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |