Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,195,151 T→G 7.1% noncoding (90/142 nt)
L4R (CTC→CGC) 
sibE ←
ibsE →
sRNA antisense regulator of toxic IbsE protein
toxic membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,195,1510TG 7.1% 133.8 / 2.6 57noncoding (90/142 nt)
L4R (CTC→CGC) 
sibE
ibsE
sRNA antisense regulator of toxic IbsE protein
toxic membrane protein
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base G (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

ACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAG  >  NC_000913/3195006‑3195295
                                                                                                                                                 |                                                                                                                                                
aCCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGt                                                                                                                                               >  1:46993/1‑149 (MQ=255)
     tCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatca                                                                                                                                          <  1:842250/149‑1 (MQ=255)
        gggAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGAAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGACGAGCTTCATCATaa                                                                                                                                       <  2:74559/149‑2 (MQ=18)
            aGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                                                                                                                                   <  1:958980/149‑1 (MQ=255)
             ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                                                                                                                                  >  2:630288/1‑149 (MQ=255)
              gCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGACGAGCTTCATCATaacccttt                                                                                                                                 <  1:377844/149‑8 (MQ=18)
                aaaaTTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtg                                                                                                                               >  2:42946/1‑149 (MQ=255)
                aaaaTTGCCTCTGGGAAACCATTGCGTGGGGCCCCGCGCTCATCAACAAACGGGGGGCACCAAGTGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGNTATGATGAAGCTCGTCATCATACTGgntgtg                                                                                                                               <  1:689981/149‑1 (MQ=18)
                   tttGCCGCTGTGAATGCTTGGCGCGGGGTCCTGCGCTGATCAAACATCGGTGTGCATCAATGTTATGAAACTGTAAATCCACTCACGAAGAAGGGGCCTTGTATAAGGTAAGGGGTACGATGAAGCTCGTCATCATACTGgttgtgttg                                                                                                                            <  1:689792/148‑1 (MQ=12)
                       ccgctGGGAAACCATTGCGCGGGGTCCGGCGCTCATCAACACTCGGTGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTAc                                                                                                                        <  2:780230/146‑1 (MQ=255)
                        ctctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt                                                                                                                       <  2:312006/149‑1 (MQ=255)
                           tGGGAAAGAATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCACTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt                                                                                                                    <  2:653079/149‑1 (MQ=255)
                             ggAAAGCATTGCGCTGGGTCCGGCGCTCATCAACAAACGGGGGTCAGCAAGGGGCTCAAACTGGAAAGCCACTCCCGCAGAAGGGGCCTTGTATAAGGAAAGCGTTATGACGACGCTCGTCAACCTACTGTTTATGTTGTTACTGTTac                                                                                                                  >  2:492948/1‑148 (MQ=255)
                             ggAAACCATTGCGGGGGGTCCGGCGCTCATCACCAAGCGGGGGGCAGCCAGGGGCTGAACCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGACAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                                                                                                  <  1:777092/149‑1 (MQ=18)
                               aaaGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt                                                                                                                >  2:538756/1‑149 (MQ=255)
                               aaaGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt                                                                                                                >  1:38257/1‑149 (MQ=255)
                                aaGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAACCGGGACAGCCCCGCCCGAAGAAGGGGCCTTGTCTAAGGAAAGGGTTATGTTGAGGCTCGTCATCATCCTGGTTGTGTGGTTACTGTTATGtt                                                                                                               >  1:609270/1‑149 (MQ=18)
                                 aGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt                                                                                                              >  2:353326/1‑149 (MQ=255)
                                  gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGTGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTc                                                                                                             <  2:180722/149‑1 (MQ=255)
                                       gcgcGGGGTCCGGCGCTCATCAACAATCGGTGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTGTAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc                                                                                                        <  2:474821/149‑1 (MQ=255)
                                       gcgcGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGCAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCgcc                                                                                                        >  1:35211/1‑147 (MQ=255)
                                               tCCTGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaac                                                                                                <  2:416925/149‑1 (MQ=255)
                                                         atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCACTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGCTTGTGTTGTTACTGTTTAGTTTCCCGACTTACTAACAACTCATCag                                                                                      >  1:212396/1‑149 (MQ=255)
                                                           caacaaTCGGGGGGCAGCACGGGGCTGAAACGGGACAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGGTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCagag                                                                                    <  2:290872/149‑1 (MQ=255)
                                                             acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggg                                                                                  >  1:878154/1‑149 (MQ=255)
                                                               aaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCAAACTGGTGGTGTTGTTAATGTTAAGTTTCCCGACTTACTAACAACTCTTCAGAggggg                                                                                >  2:424262/1‑149 (MQ=255)
                                                                 tCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGTTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGga                                                                              <  1:284242/149‑1 (MQ=255)
                                                                          tgcaAGGGGCTGACACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCAGCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAAtcctc                                                                     <  1:465419/148‑1 (MQ=255)
                                                                           ccatggggCTGAAACGGGAAAGCCCATCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAAtcctcc                                                                    <  1:58617/145‑1 (MQ=255)
                                                                            caAGGGGCTGAAACGGTAAAGCCCCTAACGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTAGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAAATCATCAGAGGGGTTATAAAtcatcca                                                                   >  2:576461/1‑148 (MQ=21)
                                                                            caAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTACCGACTTACTACCCACTCATCAGAGGGGGGAGAAATCCTccc                                                                   >  2:710607/1‑149 (MQ=255)
                                                                                  gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTAccc                                                             >  1:992935/1‑149 (MQ=255)
                                                                                    ggAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAACGGGGTATGATGAAGCTCGTCATCATACTGGTTGGGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCtt                                                           <  1:859425/148‑1 (MQ=255)
                                                                                     gAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTg                                                          <  2:800870/149‑1 (MQ=255)
                                                                                      aaaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGt                                                         >  1:829561/1‑149 (MQ=255)
                                                                                       aaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCTTCAGAGGGGGGAGAAATCCTCCCTTACGCTTGtt                                                        >  1:968378/1‑149 (MQ=255)
                                                                                             aaaGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTa                                                  >  1:525804/1‑149 (MQ=255)
                                                                                              aaGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTAc                                                 <  1:546633/149‑1 (MQ=255)
                                                                                               aGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTAct                                                <  1:281910/149‑1 (MQ=255)
                                                                                                  cccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTa                                             >  1:394100/1‑149 (MQ=255)
                                                                                                            gaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGTGGAGAAATCCTCACTTACACTTGTTCCTTTACTCTAGTTTGaaaaa                                   >  2:467926/1‑149 (MQ=255)
                                                                                                                 gtccTTGTTTAAGGTAAGGGTTATGATGATGCTCGTGATCATACTGGTTGTTTTGTTACTGTTATGTTTCGCGACTTTCTAACAACTCATCAGAGTGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaacaac                              <  2:423987/147‑1 (MQ=255)
                                                                                                                  gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAacaaca                             <  1:630288/149‑1 (MQ=255)
                                                                                                                      tGTATGAGTTAAGGGTTATGATGAAGCTCGGAATCATACTGGTTGTTTTGTTAATTTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGt                         <  2:695784/149‑1 (MQ=18)
                                                                                                                          taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAAt                     >  2:966600/1‑149 (MQ=255)
                                                                                                                           aaGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTCTTACTGATAAGTTTCCCGACTTACTAAGAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGATCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATa                    >  2:134254/1‑149 (MQ=255)
                                                                                                                            acgAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGGTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATat                   <  2:212396/147‑2 (MQ=255)
                                                                                                                             ggAAAGGGTAATGATGAAGCTCCTCATCATACAGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAATAACAACTCAACACAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACACAGTCAATAgc                  >  2:1017517/1‑148 (MQ=255)
                                                                                                                              taaaTGGTTATGATGCAGCTCGTCATAATACTGGTTGTGTTTTTACTTTTAAGTATCACGACTTACTAAACACTCATAAGAGGGGTGAGAAATACTCCCTTAACCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc                 <  1:576461/148‑1 (MQ=255)
                                                                                                                              gAAAGGGTTTTGATGAAGCTCGTCATCATACTGTTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc                 <  1:710607/149‑1 (MQ=255)
                                                                                                                               caaccagtATGATGACGAGCTTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGcc                >  1:229594/8‑149 (MQ=21)
                                                                                                                               aaaGGGTTATTATGAAGATAGTCATCATACTGTTTGTGTTGTAACTGTTAAGTTTCCCGACTTACTTACAACTAATCAGAGGGGGGAGAAATACTCACTTACCCTTGTTCCTTTACTCTAGTTTGAAAAAAAAACAGCGTCAATAGGcc                >  2:362327/1‑149 (MQ=255)
                                                                                                                                aaccagtATGATGACGAGCTTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATACTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt               >  1:815949/7‑149 (MQ=255)
                                                                                                                                       ttgatgaAGCTCGTCATCATACTGGGTGTGTTGTTACTGGTAAGTTTCCCGACTTACTAACCACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGt        <  2:473225/148‑1 (MQ=255)
                                                                                                                                         gatgaAGCTCGGCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAAATCATAAGAGGGGGGAGAAATCCTCCCTTACCATTTTTCCTATACTCTAGGTTGAAAAAACAAAAGAGTCAATAGGCCTCCCATGTAc      >  1:620990/1‑149 (MQ=21)
                                                                                                                                           tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa    >  2:262404/1‑149 (MQ=255)
                                                                                                                                             aaGCTCGTCATCATACTGGGTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACCACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg  <  2:38257/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAG  >  NC_000913/3195006‑3195295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: