Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,195,153 | G→T | 7.0% | noncoding (88/142 nt) V5F (GTC→TTC) |
sibE ← ibsE → |
sRNA antisense regulator of toxic IbsE protein toxic membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,195,153 | 0 | G | T | 7.0% | 138.3 / 2.0 | 57 | noncoding (88/142 nt) V5F (GTC→TTC) | sibE ibsE | sRNA antisense regulator of toxic IbsE protein toxic membrane protein |
Reads supporting (aligned to +/- strand): ref base G (25/27); new base T (2/2); total (28/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 |
ACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAG > NC_000913/3195006‑3195295 | aCCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGt > 1:46993/1‑149 (MQ=255) tCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatca < 1:842250/149‑1 (MQ=255) gggAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGAAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGACGAGCTTCATCATaa < 2:74559/149‑2 (MQ=18) aGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt < 1:958980/149‑1 (MQ=255) ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt > 2:630288/1‑149 (MQ=255) gCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGACGAGCTTCATCATaacccttt < 1:377844/149‑8 (MQ=18) aaaaTTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtg > 2:42946/1‑149 (MQ=255) aaaaTTGCCTCTGGGAAACCATTGCGTGGGGCCCCGCGCTCATCAACAAACGGGGGGCACCAAGTGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGNTATGATGAAGCTCGTCATCATACTGgntgtg < 1:689981/149‑1 (MQ=18) tttGCCGCTGTGAATGCTTGGCGCGGGGTCCTGCGCTGATCAAACATCGGTGTGCATCAATGTTATGAAACTGTAAATCCACTCACGAAGAAGGGGCCTTGTATAAGGTAAGGGGTACGATGAAGCTCGTCATCATACTGgttgtgttg < 1:689792/148‑1 (MQ=12) ccgctGGGAAACCATTGCGCGGGGTCCGGCGCTCATCAACACTCGGTGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTAc < 2:780230/146‑1 (MQ=255) ctctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt < 2:312006/149‑1 (MQ=255) tGGGAAAGAATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCACTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 2:653079/149‑1 (MQ=255) ggAAAGCATTGCGCTGGGTCCGGCGCTCATCAACAAACGGGGGTCAGCAAGGGGCTCAAACTGGAAAGCCACTCCCGCAGAAGGGGCCTTGTATAAGGAAAGCGTTATGACGACGCTCGTCAACCTACTGTTTATGTTGTTACTGTTac > 2:492948/1‑148 (MQ=255) ggAAACCATTGCGGGGGGTCCGGCGCTCATCACCAAGCGGGGGGCAGCCAGGGGCTGAACCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGACAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa < 1:777092/149‑1 (MQ=18) aaaGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt > 2:538756/1‑149 (MQ=255) aaaGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt > 1:38257/1‑149 (MQ=255) aaGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAACCGGGACAGCCCCGCCCGAAGAAGGGGCCTTGTCTAAGGAAAGGGTTATGTTGAGGCTCGTCATCATCCTGGTTGTGTGGTTACTGTTATGtt > 1:609270/1‑149 (MQ=18) aGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt > 2:353326/1‑149 (MQ=255) gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGTGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTc < 2:180722/149‑1 (MQ=255) gcgcGGGGTCCGGCGCTCATCAACAATCGGTGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTGTAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc < 2:474821/149‑1 (MQ=255) gcgcGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGCAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCgcc > 1:35211/1‑147 (MQ=255) tCCTGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaac < 2:416925/149‑1 (MQ=255) atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCACTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGCTTGTGTTGTTACTGTTTAGTTTCCCGACTTACTAACAACTCATCag > 1:212396/1‑149 (MQ=255) caacaaTCGGGGGGCAGCACGGGGCTGAAACGGGACAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGGTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCagag < 2:290872/149‑1 (MQ=255) acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggg > 1:878154/1‑149 (MQ=255) aaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCAAACTGGTGGTGTTGTTAATGTTAAGTTTCCCGACTTACTAACAACTCTTCAGAggggg > 2:424262/1‑149 (MQ=255) tCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGTTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGga < 1:284242/149‑1 (MQ=255) tgcaAGGGGCTGACACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCAGCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAAtcctc < 1:465419/148‑1 (MQ=255) ccatggggCTGAAACGGGAAAGCCCATCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAAtcctcc < 1:58617/145‑1 (MQ=255) caAGGGGCTGAAACGGTAAAGCCCCTAACGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTAGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAAATCATCAGAGGGGTTATAAAtcatcca > 2:576461/1‑148 (MQ=21) caAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTACCGACTTACTACCCACTCATCAGAGGGGGGAGAAATCCTccc > 2:710607/1‑149 (MQ=255) gCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTAccc > 1:992935/1‑149 (MQ=255) ggAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAACGGGGTATGATGAAGCTCGTCATCATACTGGTTGGGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCtt < 1:859425/148‑1 (MQ=255) gAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTg < 2:800870/149‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGt > 1:829561/1‑149 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCTTCAGAGGGGGGAGAAATCCTCCCTTACGCTTGtt > 1:968378/1‑149 (MQ=255) aaaGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTa > 1:525804/1‑149 (MQ=255) aaGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTAc < 1:546633/149‑1 (MQ=255) aGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTAct < 1:281910/149‑1 (MQ=255) cccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTa > 1:394100/1‑149 (MQ=255) gaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGTGGAGAAATCCTCACTTACACTTGTTCCTTTACTCTAGTTTGaaaaa > 2:467926/1‑149 (MQ=255) gtccTTGTTTAAGGTAAGGGTTATGATGATGCTCGTGATCATACTGGTTGTTTTGTTACTGTTATGTTTCGCGACTTTCTAACAACTCATCAGAGTGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaacaac < 2:423987/147‑1 (MQ=255) gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAacaaca < 1:630288/149‑1 (MQ=255) tGTATGAGTTAAGGGTTATGATGAAGCTCGGAATCATACTGGTTGTTTTGTTAATTTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGt < 2:695784/149‑1 (MQ=18) taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAAt > 2:966600/1‑149 (MQ=255) aaGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTCTTACTGATAAGTTTCCCGACTTACTAAGAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGATCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATa > 2:134254/1‑149 (MQ=255) acgAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGGTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATat < 2:212396/147‑2 (MQ=255) ggAAAGGGTAATGATGAAGCTCCTCATCATACAGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAATAACAACTCAACACAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACACAGTCAATAgc > 2:1017517/1‑148 (MQ=255) taaaTGGTTATGATGCAGCTCGTCATAATACTGGTTGTGTTTTTACTTTTAAGTATCACGACTTACTAAACACTCATAAGAGGGGTGAGAAATACTCCCTTAACCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc < 1:576461/148‑1 (MQ=255) gAAAGGGTTTTGATGAAGCTCGTCATCATACTGTTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc < 1:710607/149‑1 (MQ=255) caaccagtATGATGACGAGCTTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGcc > 1:229594/8‑149 (MQ=21) aaaGGGTTATTATGAAGATAGTCATCATACTGTTTGTGTTGTAACTGTTAAGTTTCCCGACTTACTTACAACTAATCAGAGGGGGGAGAAATACTCACTTACCCTTGTTCCTTTACTCTAGTTTGAAAAAAAAACAGCGTCAATAGGcc > 2:362327/1‑149 (MQ=255) aaccagtATGATGACGAGCTTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATACTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt > 1:815949/7‑149 (MQ=255) ttgatgaAGCTCGTCATCATACTGGGTGTGTTGTTACTGGTAAGTTTCCCGACTTACTAACCACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGt < 2:473225/148‑1 (MQ=255) gatgaAGCTCGGCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAAATCATAAGAGGGGGGAGAAATCCTCCCTTACCATTTTTCCTATACTCTAGGTTGAAAAAACAAAAGAGTCAATAGGCCTCCCATGTAc > 1:620990/1‑149 (MQ=21) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGa > 2:262404/1‑149 (MQ=255) aaGCTCGTCATCATACTGGGTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACCACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAg < 2:38257/149‑1 (MQ=255) | ACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAG > NC_000913/3195006‑3195295 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |