Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,917 T→C 7.9% intergenic (+315/+299) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9170TC 7.9% 152.4 / 2.5 68intergenic (+315/+299)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base T (12/45);  new base C (2/3);  total (18/50)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGT  >  NC_000913/3269770‑3270032
                                                                                                                                                   |                                                                                                                   
gAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtg                                                                                                                    <  1:49760/149‑1 (MQ=255)
 aaTTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtgt                                                                                                                   >  2:102252/1‑149 (MQ=255)
  aTTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc                                                                                                                  <  2:788621/149‑1 (MQ=255)
    ttGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCCGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt                                                                                                                <  1:640303/149‑1 (MQ=255)
    ttGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt                                                                                                                <  1:970025/149‑1 (MQ=255)
     tGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCgag                                                                                                               >  2:348048/1‑147 (MQ=255)
       cGTGCTTTTGTAGGCAGTATAAGGCGTTCACGCCGCATCCGGAATGAATAAAGCGCACTCAACAATCTGAAACCCGCAGGAACGGTTTATTACGTACATCAGGTAAAACTGAACGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                                                             <  2:936903/149‑1 (MQ=255)
        gTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                                                            >  1:985348/1‑149 (MQ=255)
        gTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTCGAc                                                                                                            >  1:138848/1‑149 (MQ=255)
         tGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACa                                                                                                           <  1:303335/149‑1 (MQ=255)
         tGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCCGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACa                                                                                                           >  1:195781/1‑149 (MQ=255)
          gCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTgagctg                                                                                                          >  1:115953/1‑144 (MQ=255)
          gCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAg                                                                                                          >  1:391298/1‑149 (MQ=255)
            ttttGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTTAAACTGACAGATCAGCAGCTTTCTGTTGGCTATAGAGTCGTGGGGAGtc                                                                                                        >  1:734688/1‑149 (MQ=37)
                gTAGGCAGGATAAGGCGTTCACGCAGCATCCGGCATGAATAAAGCGCTCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattc                                                                                                    <  1:60508/149‑1 (MQ=255)
                 tAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattca                                                                                                   >  2:327056/1‑149 (MQ=255)
                   ggCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAAGAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATACGCCGCGTTCTTTTGGGTATAGGGTCGTGGGCAGtccttcatg                                                                                                 >  1:402098/1‑148 (MQ=38)
                   ggCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATc                                                                                                 <  1:185110/149‑1 (MQ=255)
                    gCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCATCt                                                                                                >  1:7177/1‑149 (MQ=255)
                          tAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGc                                                                                          <  2:900941/149‑1 (MQ=255)
                              gCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTAAAACCCGCCGGAACGGTTTATTACGTACATAAGGTAAAACTGACCGATAAGCCGCATTATTTTGTGTATAGTGTCTTGGACAGTCCATGATCTTTGTGacccg                                                                                      >  2:951182/1‑148 (MQ=37)
                                gTTCACGCCGCATCCGGCATGAATAAAGAGCAATCAACAATCTGAAACCCGCCGGAACGGTTTATAACTTAAATCAGGTAAAACTGCCCGAAAAGCCGCTTTATTTTGGGTATTGAGTCGTGGAAACTCATGAATCTTTCTGccacaac                                                                                    >  2:549175/1‑144 (MQ=255)
                                    aCGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                                <  1:834717/149‑1 (MQ=255)
                                      gccgcATCCGGCATTAATAAAGCGCACTAAACAATCTGAAAGCCGGCGGAACGGTTTATTACGGACATCAGGGAAAACTGACCGATAAGCCGCTTTCTTTTGTGTAAAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt                                                                              <  1:338322/149‑1 (MQ=21)
                                      gccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt                                                                              <  1:345436/149‑1 (MQ=255)
                                      gccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGACATTCATCATTCAGCCCCTCCAAAAGt                                                                              >  2:338322/1‑149 (MQ=255)
                                      gccgcATCCGGCATGAATAAAGCGAACTCAATAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTATAACTGACCGTTAAGCCGATTTCTTATGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt                                                                              <  2:561576/149‑1 (MQ=21)
                                        cgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaa                                                                            <  1:806223/149‑1 (MQ=255)
                                         gcATCCGGCATGAATAAAGCGCACTCAACACTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                                           <  2:580081/149‑1 (MQ=255)
                                         gcAGCCGGCATGAATAAAGCGCACTCAACAATCTGAACCCCGCCGGAACGGATTATTACTTACATCAGGTAAAACTGACCGATAAGCCGCTTTCATTTGGGTAAAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                                           <  2:286470/149‑1 (MQ=21)
                                            tCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTAATACGTACATCAGGTAAAAATCACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATAAAGCTTTCGGCCCCACCAAAAGAAAAAAc                                                                        >  1:676976/1‑149 (MQ=21)
                                            tCCGGCATGAATAAAGAGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCATGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAc                                                                        <  1:688973/149‑1 (MQ=255)
                                              cGGCATGAATAAAGCGCACTAAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAccc                                                                      <  1:304286/149‑1 (MQ=255)
                                                          ctcgcACTCCAAAATCTTAAACCCGCCGGATCGGTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                          <  1:594292/147‑1 (MQ=11)
                                                            cgcACTCAACAATCAGAAACCCGCCGGAACGGTTTATTACGTACATCAGGGAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttt                                                        <  1:471696/149‑1 (MQ=255)
                                                             gcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTAATTCATAATTCTGCCCCTCCAAAAGCAAAAACCCTCCGAAGCGGGttag                                                       >  2:594292/1‑147 (MQ=17)
                                                              cACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCATAAGTAAAAACCCGCCGAAGCGGGttttt                                                      <  1:81439/149‑1 (MQ=255)
                                                               aCTCAACAATCTGCAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTa                                                     <  2:384074/149‑1 (MQ=255)
                                                               aCTCAACAATCTAAAACCCGCCGTATCGGGTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                     <  2:402098/149‑1 (MQ=11)
                                                                cTCATCAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGGCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                    <  1:284111/149‑1 (MQ=255)
                                                                   aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                 >  1:452721/1‑149 (MQ=255)
                                                                    acaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                <  1:956376/149‑1 (MQ=255)
                                                                     caaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                               <  2:719776/149‑1 (MQ=255)
                                                                      aaTCTGAAACCCGCCGGATCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaat                                              <  1:327056/149‑2 (MQ=255)
                                                                         cTGACACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAg                                           <  2:681727/149‑1 (MQ=255)
                                                                         cTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAg                                           <  1:88860/149‑1 (MQ=255)
                                                                          tGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                                          <  1:771319/149‑1 (MQ=255)
                                                                           gcaaCCCGCCGGAACGGATTATTACGTACATCAGGGAACACTGACCGATAAGCCGCTGTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGt                                         <  1:564666/147‑1 (MQ=255)
                                                                           gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGt                                         <  1:304986/149‑1 (MQ=255)
                                                                            aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                                        <  2:1007849/149‑1 (MQ=255)
                                                                             aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGa                                       <  2:985348/149‑1 (MQ=255)
                                                                               cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                     <  2:378837/149‑1 (MQ=255)
                                                                               cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                     <  1:136658/149‑1 (MQ=255)
                                                                                ccgccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAc                                    <  2:317864/149‑1 (MQ=255)
                                                                                 cgccgGAACTGTTTATTACGTACATCTGGTAAATCTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCTTTGACAGTCATTCATCTTTCTTCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAATACAGGTGAAACt                                   <  2:985558/149‑1 (MQ=17)
                                                                                    cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                <  2:428784/149‑1 (MQ=255)
                                                                                     ggAACGGTTTATTTCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGTTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                               <  1:910438/149‑1 (MQ=255)
                                                                                       aaCGGTTTATTACGTACATCATGTAATACTGACCGATATGCCGCTTTGTTTTGGGTTTAGTGTCGTGGACAGTCATTAATCTTTCTGCCCCTGCAAAAGTAAAAACCCGCGGATGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGa                             <  2:548660/149‑1 (MQ=17)
                                                                                        aaggTTTATTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                            <  1:951182/147‑1 (MQ=255)
                                                                                        aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt                            <  2:108729/149‑1 (MQ=255)
                                                                                                      acttCAGGTATAAGAGAGCGATATGCCGCTTTCTTTTGTTTATTGTGTCGTCGAAAGTGTTTCATATTTATGCCACTCAAAAATTATAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGctttcttt              <  2:138848/146‑1 (MQ=2)
                                                                                                      aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCt                                                                                                <  1:284687/67‑1 (MQ=255)
                                                                                                      aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAATTCATCt                                                                                                >  2:284687/1‑67 (MQ=255)
                                                                                                       cATCAGGTAAAACTGACCGTTAAGACGATTTCTTTTGGTTATAGTGTCGTGGACAGTAATTCATCTTTATGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt             <  1:30258/149‑1 (MQ=14)
                                                                                                        atcatttAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg            <  1:742863/143‑1 (MQ=16)
                                                                                                         tCAGGTATAACTGACCGATATGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg           <  1:348048/149‑1 (MQ=255)
                                                                                                         tCAGGTAAAACTGACCGATAAGCCGTATTCTTTTGTGGATAGTGTCGTGGACAGTCATTCATCTATCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg           <  2:998852/149‑1 (MQ=11)
                                                                                                         aaacgTAAAACTGACCGATAAGCCGCTTTCTTTTGGCTATAGTCTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg           <  2:126164/145‑1 (MQ=255)
                                                                                                          aagttAAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg          <  1:53891/145‑1 (MQ=255)
                                                                                                                  aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATAATTCTGCCCCTCAAAAAGAAAAAACCAGCCGAAGCGGGTTTTTACTTAAAACAGGTTAAAATGACCCATAATCCGGGTTATTTTGGGTAAAgggt  >  2:801605/1‑149 (MQ=11)
                                                                                                                                                   |                                                                                                                   
GAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGT  >  NC_000913/3269770‑3270032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: