Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,276 T→A 5.2% intergenic (‑127/+38) rplN ← / ← rpsQ 50S ribosomal subunit protein L14/30S ribosomal subunit protein S17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,2760TA 5.2% 198.2 / 2.5 77intergenic (‑127/+38)rplN/rpsQ50S ribosomal subunit protein L14/30S ribosomal subunit protein S17
Reads supporting (aligned to +/- strand):  ref base T (38/35);  new base A (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

TTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTC  >  NC_000913/3448138‑3448422
                                                                                                                                          |                                                                                                                                                  
ttCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGt                                                                                                                                          <  1:983637/149‑1 (MQ=255)
 tCTTGGATCATTTTAGAGCTCCGCTAATGTCAACTACTACTGAGACCCGAAACTCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGtt                                                                                                                                         <  1:954829/149‑1 (MQ=255)
   ttGGATCATATTAGTGCTCCGATAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTAAAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTa                                                                                                                                       <  2:90025/149‑1 (MQ=255)
    tGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGTTCTGAGCCGTTTAt                                                                                                                                      >  2:860590/1‑149 (MQ=255)
     ggATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTAtt                                                                                                                                     <  1:34548/149‑1 (MQ=255)
      gATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTc                                                                                                                                    <  2:850985/149‑1 (MQ=255)
      gATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTc                                                                                                                                    >  1:427515/1‑149 (MQ=255)
         cATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATCTCGAGGGCGCGGCATTATAACACCGCTTCACGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGTTTAtttttt                                                                                                                                 <  1:922279/149‑5 (MQ=255)
             ttAGTGCTCCGCTAATGTCAACTCCTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCCAGGGCGCGGCATTAAAACACCGCTTCAAGGATATGGGTAGAAAAACTAACCGGCTCATTTCTGAGCCGTTTATTCGTATTga                                                                                                                             <  1:970092/149‑1 (MQ=255)
                  gCTCCGCTAATGTCAACTACTACTGATACCAGAAAATCATGTCGTTAAAAATCACCATATCGAGGGAGCGTCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGagagag                                                                                                                        <  2:887564/149‑1 (MQ=255)
                   cTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgt                                                                                                                       >  1:631712/1‑149 (MQ=255)
                     cctcTAATGTCAACTACTACGGATACACGAAAATCAGGTCGTTAAAAATCCCCATATCGATGGCGCTGCATTATAACACCTCTTCAAGTATATGGTTAGAAAAAATAAACGGCTCCGTTCTGAGCCTGTTAATCGTATTGAGAGAgtgt                                                                                                                     >  2:413044/4‑149 (MQ=255)
                     ccGCAAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCACATTTCTGAGCCGTTTATTCGTATTGAGAGAgtgt                                                                                                                     <  1:318107/149‑1 (MQ=255)
                        cTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACt                                                                                                                  <  1:331411/149‑1 (MQ=255)
                           aTGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTa                                                                                                               <  2:721646/149‑1 (MQ=255)
                              tCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCCAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTa                                                                                                            <  2:481705/149‑1 (MQ=255)
                              tCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCACATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTa                                                                                                            <  2:624617/149‑1 (MQ=255)
                                  ctactactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGa                                                                                                        >  1:316604/1‑149 (MQ=255)
                                   tactactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATGCGTATTGAGAGAGTGTACTGTATTACAGaa                                                                                                       <  1:95803/149‑1 (MQ=255)
                                   tactactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTATGCGCCGTTTATTCGTATTGAGAGCGTGTACTGTATTACAGaa                                                                                                       >  2:701189/1‑149 (MQ=255)
                                       actGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGc                                                                                                   <  1:917506/149‑1 (MQ=255)
                                        ctGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGCGCCGTTTATTCGTATTGAGAGCATGTACTGTATTACAGAACCgat                                                                                                  >  1:974653/1‑147 (MQ=255)
                                        ctGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTAGTATTGAGAGAGTGTACTGTATTACAGAACCGCt                                                                                                  >  1:406000/1‑149 (MQ=255)
                                         tGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCtt                                                                                                 >  2:305904/1‑149 (MQ=255)
                                             acacGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCATGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCCTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTtctc                                                                                             >  2:612895/4‑149 (MQ=255)
                                             aCCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTtctc                                                                                             <  2:192804/149‑1 (MQ=255)
                                               ccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTGATTTCTGAGCCGTTTATTCGTATTGAGAGATTGTACTGTATTACAGAACCGCTTTCTCTa                                                                                           >  1:537041/1‑149 (MQ=255)
                                               ccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTa                                                                                           >  2:423728/1‑149 (MQ=255)
                                               ccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGCCGCGGCATTATAACACCGCTTCAAGGAAATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTAATGAGAGAGTGTACTGTATTACAGAAACCCTTACTCTa                                                                                           >  2:325790/1‑149 (MQ=255)
                                                 gAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCAGTATAACCCCGCTTCAAGGATATGGGTTGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATAGGTTTTGAGAGAGTGTACTGTATTACATAACCGCTTTCGATACa                                                                                         >  2:944231/1‑149 (MQ=255)
                                                  aaaaTCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTCCAGAACCGCTTTCTCTACaa                                                                                        <  1:590553/149‑1 (MQ=255)
                                                          gTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAAcc                                                                                <  1:724139/149‑1 (MQ=255)
                                                             gTTAAAAATCCCCATATCGATGGCGCGGCATTATACCACCGCTTCAAGTTTATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACGGAACCGCTTTCTCTACAACGCGAACCAGc                                                                             <  1:1001174/149‑1 (MQ=255)
                                                             gTTAAAAATCCCCATATCGAGGGCGCGGCATTATACCACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCGGAGCCGTTTATTGGTTTTGAGAGAGTGTACTGTATTACAGCACCGCTTTCTCTACAACGCGCACCAGc                                                                             >  2:759430/1‑149 (MQ=255)
                                                             gTTAAAAATCCCCATATCGAGGGCGCGGCATAATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAATTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGc                                                                             <  1:578325/149‑1 (MQ=255)
                                                              ttAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGTTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGTGAGAGTCTACTTTATTACAGAACCGCATTGTCTACAACGCGAACCAGCg                                                                            >  1:297423/1‑149 (MQ=255)
                                                                  aaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCa                                                                        <  1:57467/149‑1 (MQ=255)
                                                                  aaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCa                                                                        <  1:262315/149‑1 (MQ=255)
                                                                   aaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAg                                                                       <  2:957660/149‑1 (MQ=255)
                                                                   aaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAg                                                                       >  1:722208/1‑149 (MQ=255)
                                                                        ccATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGATCCAGCGTCCAGGAttt                                                                  >  1:299665/1‑149 (MQ=255)
                                                                        ccATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGACGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACAAGCGTCCAGGAttt                                                                  >  2:943203/1‑149 (MQ=255)
                                                                        ccATATCGAGGGCGCGGCATTAAAACACCGCATCAAGGATATCGGTAGAAAAAATAAACGGCTCAATTCTCAGCCGTTTATTCGTATTGAGAGATTAAAATGTATTACAGAACCGCTTTCACTACAACTAGAACCAGCTTCCAGTAttt                                                                  >  1:223068/1‑149 (MQ=255)
                                                                         cATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTa                                                                 >  1:32922/1‑149 (MQ=255)
                                                                          atatCGAGGGCGCGGCATTATAACCACGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAg                                                                <  1:258960/149‑1 (MQ=255)
                                                                          atatCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAg                                                                <  2:89926/149‑1 (MQ=255)
                                                                          atatCGAGGGCGAGGCATTATAACACCGCTTCAAGGATATGGTTAGAAAAAATAAACTGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACAGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAg                                                                <  2:569665/149‑1 (MQ=255)
                                                                             tCGAGGGCGCGGCATTATAACACCGCTTCTAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCt                                                             >  1:277302/1‑149 (MQ=255)
                                                                               gAGGGCGCGGCATTATAACCCCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTg                                                           <  2:110391/149‑1 (MQ=255)
                                                                                aGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTgg                                                          >  2:222650/1‑149 (MQ=255)
                                                                                 gggCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGa                                                         >  1:377307/1‑149 (MQ=255)
                                                                                     gcgGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAGATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAAACAGCGTCCAGGATTTAGTATTGGACAGc                                                     >  2:257737/1‑149 (MQ=255)
                                                                                     gcgGCATTATAACACCCCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCAGTTTATTCGTATTGAGAGAGTGTACTGTATTACATAACCGCTTTCTCTACAACGAGAACCACCGTCAAGGATTTAGTCTTGGACAGc                                                     >  2:723220/1‑149 (MQ=255)
                                                                                          aTTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACACCGCGACCCAGCGTCCAGGATTTAGTGTTGGACAGCGGACg                                                >  2:693835/1‑149 (MQ=255)
                                                                                            tataACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGc                                              >  1:735453/1‑149 (MQ=255)
                                                                                                acacCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGACCCAGCGTCCAGGATTTAGTCTTGGGAAGCGGACGGCATTc                                          >  2:737290/1‑149 (MQ=255)
                                                                                                 cacCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTcg                                         >  1:265364/1‑149 (MQ=255)
                                                                                                 cacCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTATTAATTAGAGAGTGTACTGTATTAAATAACAGATTTCTCTACAACTAGAACAATCGTCCATGATTTAGTCTTGGACAGCGGACGGCATTcg                                         >  2:102459/1‑149 (MQ=255)
                                                                                                      cTTCAAGGATATTTGAAGAAAAATTAAACGGCTCTTTTCTTATCCGTTTATTCGTATTGAGAGATTGTATTGTATTACAGAACAGCTTTCTCTACAACGCGAACCAGAGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGAt                                    <  1:743612/149‑1 (MQ=255)
                                                                                                      cTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCTCTTTCTCTACAACGCGAACCAGCGTCCAGGAGTTAGTCTTGGACAGCGGACGGCATTCGCGGAt                                    >  2:459179/1‑149 (MQ=255)
                                                                                                       ttCAAGGATATGGGTAGAAAAAATAAACGGCTCAATTCTGAGCCGTTTATTCGAATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGACCAGGATTAATTCTTGAACAGCGGACGCCATTCGCGGAtt                                   >  1:111833/1‑149 (MQ=255)
                                                                                                             gATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAAcc                             <  1:612895/149‑1 (MQ=255)
                                                                                                               tatGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTTGACAGCGGACGGCATTCGCGGATTTCAACCAc                           <  2:31191/149‑1 (MQ=255)
                                                                                                                  gggTATAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTc                        <  1:325790/149‑1 (MQ=255)
                                                                                                                  gggTAGAAAAAATAAACGGCTCATTTCTGCGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAAAGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTc                        >  1:695973/1‑149 (MQ=255)
                                                                                                                    gTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCAc                      >  2:554878/1‑149 (MQ=255)
                                                                                                                    gTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCAc                      <  2:789906/149‑1 (MQ=255)
                                                                                                                    gTAGAAAAAATAAACGGCTCATTTCTGAGCCGATTATTCGTATTGAGAGACGGTACTGTATTACAGAACCGCTTTCTCTACCACGCGAACCTGCGTCCAGGATTTAGTATAGGACTGCGGACGTCATTCGCGGAGTTCACCCACGTccc                      >  1:814094/1‑147 (MQ=255)
                                                                                                                        aaaaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGAATAGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGAt                  <  2:88305/149‑1 (MQ=255)
                                                                                                                            aaTAAACGGCTCAGAAATGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGc             >  1:390280/1‑149 (MQ=255)
                                                                                                                              tAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCa            <  2:457756/149‑1 (MQ=255)
                                                                                                                              tAAACGGCTCAGAAATGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTGTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCa            >  1:261788/1‑149 (MQ=255)
                                                                                                                                 aCGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTc         <  1:701189/149‑1 (MQ=255)
                                                                                                                                   ggCCCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgt       <  2:824144/149‑1 (MQ=255)
                                                                                                                                     cTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGGAGTACAGCACCGCTTTCTCTACAACGAGAACAAGCGGCCAGGTTTTAGTCTTGTACAGAGGACTACCTTCTCGCATTTAACACACTTCCCCGATACCGCATACgttg     >  2:574006/1‑149 (MQ=255)
                                                                                                                                        aTTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTtc  >  1:438717/1‑149 (MQ=255)
                                                                                                                                        aTTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTtc  <  1:305904/149‑1 (MQ=255)
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TTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTC  >  NC_000913/3448138‑3448422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: