Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,817,940 T→A 5.5% F61I (TTC→ATC)  dinD → DNA damage‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,9400TA 5.5% 328.3 / 4.6 119F61I (TTC→ATC) dinDDNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base T (61/51);  new base A (5/2);  total (66/53)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 123 total aligned reads displayed.

AAAACTGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGC  >  NC_000913/3817792‑3818088
                                                                                                                                                    |                                                                                                                                                    
aaaaCTGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTTGCCACACAATTGATGACCAtt                                                                                                                                                      >  1:614645/1‑149 (MQ=255)
  aaCTGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGACACACAATTGATGACCATTTc                                                                                                                                                    <  1:491331/149‑1 (MQ=255)
   aCTGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCg                                                                                                                                                   >  1:767971/1‑149 (MQ=255)
    cTGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAGACTACTGGGTTATTCAGAGTATCGTCACTGTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCTTTTCgg                                                                                                                                                  >  2:2395/1‑148 (MQ=255)
    cTGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGGCACTTTATACCTGTATCAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCga                                                                                                                                                  <  2:867735/149‑1 (MQ=255)
     tGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATATCgag                                                                                                                                                 >  2:959092/1‑149 (MQ=255)
      gATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCgagg                                                                                                                                                >  2:387120/1‑149 (MQ=255)
        ttAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGCAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCgaggag                                                                                                                                              >  2:433430/1‑149 (MQ=255)
                aaCGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGa                                                                                                                                      >  2:532080/1‑149 (MQ=255)
                  aggtgCATAACAATGCTCACGAAGACAACATGGGAAACTACTGGGTTATTCAGAGTATCGTCACTATATACGTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGTCCATTTCGAGGAGATCCTCGata                                                                                                                                    <  1:222355/145‑1 (MQ=255)
                       cAGAACAATGGTCAGCAAGACAACTTGGGATACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTc                                                                                                                               <  1:422172/149‑1 (MQ=255)
                           acacTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTCTCGTCACTTTATACCTGTATTAACGCGCCCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGAAGACCATATCGAGGATCTCCTCGAAATGGTCatca                                                                                                                           <  1:259138/149‑4 (MQ=255)
                           aCAATCGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCAGTGAGAACAGTGGTCACACAATTGAAGACCATATCGAGGATCACCTCGAAATGGTCatca                                                                                                                           <  1:262982/149‑4 (MQ=255)
                            cAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTAACACAAATCATGACCATTTCGAGGAGATCCTCGATATGGTCAAAAt                                                                                                                          >  1:205029/1‑149 (MQ=255)
                              aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGGCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTg                                                                                                                        <  2:665039/149‑1 (MQ=255)
                              aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTgg                                                                                                                       <  2:363983/149‑1 (MQ=255)
                                ggTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGc                                                                                                                      >  2:378868/1‑149 (MQ=255)
                                    aGCAAGACAACTTGGGAATCTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGGATTAACGCGCGCCATAGAAGCCTGTGAGAACAGTGGTCACAGAATTGATGTCCATTTCGAGGAGCTCGTCGATTTGGTCAAAATTGGCTCaa                                                                                                                  >  2:111667/1‑149 (MQ=255)
                                     gCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaaa                                                                                                                 <  1:548035/149‑1 (MQ=255)
                                      catgACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGGATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAt                                                                                                                >  1:336674/4‑149 (MQ=255)
                                      cAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAt                                                                                                                >  2:966048/1‑149 (MQ=255)
                                           cAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaa                                                                                                           >  1:418607/1‑149 (MQ=255)
                                           cAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGAGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaa                                                                                                           >  1:414640/1‑149 (MQ=255)
                                            aaCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCGAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCaaaa                                                                                                          >  2:331268/1‑149 (MQ=255)
                                             aCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaaaa                                                                                                         <  2:73844/149‑1 (MQ=255)
                                              cTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAAGCCaaaat                                                                                                        >  1:211340/1‑148 (MQ=255)
                                               ttGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAaga                                                                                                       >  1:169689/1‑149 (MQ=255)
                                                tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAagag                                                                                                      <  1:832328/149‑1 (MQ=255)
                                                 gggAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGc                                                                                                     >  2:80110/1‑149 (MQ=255)
                                                   gAAACTAATGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCTCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCTATGAGATCCTCGATATGGACAAAATTGTCTCACATGCCAAAAGAGCAt                                                                                                   >  2:581710/1‑149 (MQ=255)
                                                    aaaCTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGGCAAAATTGGCTCAAATGCCAAAAGAGCAtt                                                                                                  <  2:492206/149‑1 (MQ=255)
                                                      actactGGGTTATTCAGAGTATCTTCACTTTATACCTTTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaa                                                                                                <  1:358740/149‑1 (MQ=255)
                                                        tactGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAATCCTGTGAGATCAGTGGTCACACTATTGATGACCATTTCGAGGAGATCCTCGAGATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaaa                                                                                              >  1:682711/1‑149 (MQ=255)
                                                           tGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGAc                                                                                           >  1:1009868/1‑149 (MQ=255)
                                                            gggTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                          <  2:869524/149‑1 (MQ=255)
                                                               ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTTATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                       <  2:27580/149‑1 (MQ=255)
                                                               ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                       >  2:614871/1‑149 (MQ=255)
                                                               ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                       >  1:27639/1‑149 (MQ=255)
                                                                 aTTCAGAGTATCGTCACTTTATACCTGTATTAAAGCGCGCCAAAGTAGCCTGTGAGAACAGTGTTCACACTATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAATTGCCAAAAGAGCATTAAAAGACATCGTa                                                                                     <  2:363907/149‑1 (MQ=255)
                                                                  ttCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTAc                                                                                    >  2:57722/1‑149 (MQ=255)
                                                                   tCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGCTCCTCGATATGGGCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTAct                                                                                   >  2:792164/1‑149 (MQ=255)
                                                                     agagTATCGTCACTTTATACCTGTATTAACGCGCGCCTAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGCTCATTAAAAGCCATCTTActct                                                                                 >  2:819619/1‑149 (MQ=255)
                                                                      gagTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATTTGGTCAAAATTGTTTCAAATGACAAAAGAGCATTAAAAGACATCGTACtctc                                                                                >  1:711423/1‑149 (MQ=255)
                                                                       agTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTcc                                                                               >  2:252855/1‑149 (MQ=255)
                                                                       agTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTcc                                                                               <  1:826574/149‑1 (MQ=255)
                                                                         tATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCAGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGTAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCg                                                                             <  2:74995/149‑1 (MQ=255)
                                                                                 cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                     <  2:179260/149‑1 (MQ=255)
                                                                                 cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                     <  2:338348/149‑1 (MQ=255)
                                                                                  tttATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTg                                                                    >  2:340417/1‑149 (MQ=255)
                                                                                     ataCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAACGACATCGTACTCTCACTCTATGCCTGTTa                                                                 >  2:921737/1‑149 (MQ=255)
                                                                                       aCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTagc                                                               >  1:866687/1‑147 (MQ=255)
                                                                                       aCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                               >  1:876209/1‑149 (MQ=255)
                                                                                        ccTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCt                                                              <  2:237221/149‑1 (MQ=255)
                                                                                         cTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                             >  2:143205/1‑149 (MQ=255)
                                                                                          tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                          >  1:778400/1‑119 (MQ=255)
                                                                                          tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                          <  2:778400/119‑1 (MQ=255)
                                                                                          tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTgg                                                            <  1:378868/149‑1 (MQ=255)
                                                                                           gTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                           <  1:262927/149‑1 (MQ=255)
                                                                                           gTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                           <  2:836692/149‑1 (MQ=255)
                                                                                            tATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgta                                                          <  1:818858/149‑1 (MQ=255)
                                                                                             aTTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtag                                                         <  2:301730/149‑1 (MQ=255)
                                                                                              ttAACGCGCGCCAAAGAAGCCTGGGAGAACAGTTGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCAGgtagt                                                        <  1:545195/149‑1 (MQ=255)
                                                                                               tAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagta                                                       <  1:532080/149‑1 (MQ=255)
                                                                                                aaCGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAc                                                      <  1:501182/149‑1 (MQ=255)
                                                                                                  cgcgcgCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCAAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaa                                                    <  2:767971/149‑1 (MQ=255)
                                                                                                   gcgcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaaa                                                   <  2:253827/149‑1 (MQ=255)
                                                                                                     gcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCATCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAAc                                                 >  1:346681/1‑149 (MQ=255)
                                                                                                         cAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCg                                             >  2:419260/1‑149 (MQ=255)
                                                                                                          aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGa                                            <  1:30879/149‑1 (MQ=255)
                                                                                                          aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGCACAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATCCCTGTAACCTGGTAGTACAAAACGGCGa                                            >  1:171444/1‑149 (MQ=255)
                                                                                                           aagaagCCTGTGAGAACAGTGGTCACAGAATTGATGACCATTTCTAGGAGATCCTCGATATGGTCAATATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAc                                           <  2:538533/149‑1 (MQ=255)
                                                                                                                tccTGTGAGAACAGTGGTCACTCAATTGATGTCTATTTTGAGTAGCTCGTCGATATGGTGATAATTGGCTCAAATGCCAAAAGTGTATTAAAAGACATCGTACTCTCGCGCTATGCCTGTTACCTGGTAGTACAAATCGGCGACCCTGc                                      <  2:500899/148‑1 (MQ=255)
                                                                                                                    gtgAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGaaa                                  >  1:32177/1‑149 (MQ=255)
                                                                                                                     ggagaACAGGGGTCACACAATTGAAGACCATTTCGATGAGATCCTAGTTATGGTCAAAATTGGCGCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAAc                                 <  1:738246/148‑1 (MQ=255)
                                                                                                                      gagaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCTTTAAAAGACATCGTAGTCTCCCTCTATGCGTGTTTCCTGGTAGTACAAAACGGCGACCCTGCGAATcc                                >  2:195166/1‑149 (MQ=255)
                                                                                                                       ataaaaGTGGTCAAACAATTTATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGATCAAATTCCAAAAGAGCATTAAAAGACATCCTACTCTCCCGCTATGCCTGTTAACTGGTAGTACAAAAAGGCGACCCTGCGAAACCg                               <  2:684630/144‑1 (MQ=255)
                                                                                                                       agaACAGTGGTCACACAATTGATGACCATTTAGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAAGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCg                               <  1:57722/149‑1 (MQ=255)
                                                                                                                        gaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCgg                              <  1:501136/149‑1 (MQ=255)
                                                                                                                         aaCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAGAAAACGGCGACCCTGCGAAACCGGt                             >  1:911864/1‑149 (MQ=255)
                                                                                                                          aCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTc                            >  1:413533/1‑149 (MQ=255)
                                                                                                                           cAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCa                           >  1:448328/1‑149 (MQ=255)
                                                                                                                                gTCACATTATTGATGACCTTTTCGAGGAGATGCTGGATATGGTCAATCTAGGCTCAAATGCCATAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGGTACCTGGTTGTCCAAAACGGCGACCCTGCGAAACCGGTCATTGCg                      <  1:486191/149‑1 (MQ=255)
                                                                                                                                 tCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCgg                     >  1:1001516/1‑149 (MQ=255)
                                                                                                                                   acacaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggca                   <  1:80110/149‑1 (MQ=255)
                                                                                                                                     acaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATTACTAAAGAGAATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAGATGGTAGTACAAAACGGCGACCCTGCGCATCCGGTAATTGCggcagg                 >  1:487370/1‑149 (MQ=255)
                                                                                                                                     acaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagg                 >  1:258997/1‑149 (MQ=255)
                                                                                                                                      caATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcaggg                <  2:545023/149‑1 (MQ=255)
                                                                                                                                        aTTGATGACCATTGCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggca              <  2:614645/149‑1 (MQ=255)
                                                                                                                                             tgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAATAGCATTAAAAGACATCGTACTATACCGCTATGCCTGTTACATGGTAGTACAAAACGGCTACCCTGCGATACCGGTCATTGCGGAAGGGCAGAcat         >  2:851597/1‑147 (MQ=255)
                                                                                                                                             tgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGCCCCTGCGAATCCGGTCATTGCGGCAGGGCAGACtt         >  1:407031/1‑149 (MQ=255)
                                                                                                                                              gaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTa        >  2:48204/1‑149 (MQ=255)
                                                                                                                                              gaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGTAACCGGTCATTGCGGCAGGGCAGACGTa        >  1:989504/1‑149 (MQ=255)
                                                                                                                                               aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGCCCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt       >  1:315994/1‑149 (MQ=255)
                                                                                                                                               aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt       >  1:945617/1‑149 (MQ=255)
                                                                                                                                               aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt       >  2:973407/1‑149 (MQ=255)
                                                                                                                                                 cATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAttt     >  1:20244/1‑149 (MQ=255)
                                                                                                                                                  aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtttt    >  1:266301/1‑149 (MQ=255)
                                                                                                                                                  aTTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtttt    <  2:43630/149‑1 (MQ=255)
                                                                                                                                                   tttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAATGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTg   <  1:1019759/149‑1 (MQ=255)
                                                                                                                                                    ttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGc  >  1:430970/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAAACTGATTAATGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGC  >  NC_000913/3817792‑3818088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: