Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,817,949 A→C 5.6% I64L (ATC→CTC)  dinD → DNA damage‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,817,9490AC 5.6% 312.2 / 2.6 119I64L (ATC→CTC) dinDDNA damage‑inducible protein
Reads supporting (aligned to +/- strand):  ref base A (55/55);  new base C (6/3);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAC  >  NC_000913/3817804‑3818097
                                                                                                                                                 |                                                                                                                                                    
tGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATcc                                                                                                                                                   >  2:355924/1‑149 (MQ=255)
  cAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTc                                                                                                                                                 >  2:254463/1‑149 (MQ=255)
    aaCGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGa                                                                                                                                               >  2:532080/1‑149 (MQ=255)
      aggtgCATAACAATGCTCACGAAGACAACATGGGAAACTACTGGGTTATTCAGAGTATCGTCACTATATACGTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGTCCATTTCGAGGAGATCCTCGata                                                                                                                                             <  1:222355/145‑1 (MQ=255)
        gAGCAGAACAATGGTCAGCAAGACAAATTGTGATACTACTGTGTTATTCAGAGTATCGTCACTTTTTACCTGTATTAACGCGCGCCATAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATg                                                                                                                                           <  1:839032/149‑1 (MQ=255)
           cAGAACAATGGTCAGCAAGACAACTTGGGATACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTc                                                                                                                                        <  1:422172/149‑1 (MQ=255)
               acacTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTCTCGTCACTTTATACCTGTATTAACGCGCCCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGAAGACCATATCGAGGATCTCCTCGAAATGGTCatca                                                                                                                                    <  1:259138/149‑4 (MQ=255)
               aCAATCGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCAGTGAGAACAGTGGTCACACAATTGAAGACCATATCGAGGATCACCTCGAAATGGTCatca                                                                                                                                    <  1:262982/149‑4 (MQ=255)
                cAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTAACACAAATCATGACCATTTCGAGGAGATCCTCGATATGGTCAAAAt                                                                                                                                   >  1:205029/1‑149 (MQ=255)
                  aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGGCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTg                                                                                                                                 <  2:665039/149‑1 (MQ=255)
                  aTGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTgg                                                                                                                                <  2:363983/149‑1 (MQ=255)
                    ggTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGc                                                                                                                               >  2:378868/1‑149 (MQ=255)
                        aGCAAGACAACTTGGGAATCTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGGATTAACGCGCGCCATAGAAGCCTGTGAGAACAGTGGTCACAGAATTGATGTCCATTTCGAGGAGCTCGTCGATTTGGTCAAAATTGGCTCaa                                                                                                                           >  2:111667/1‑149 (MQ=255)
                         gCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCaaa                                                                                                                          <  1:548035/149‑1 (MQ=255)
                          catgACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGGATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAt                                                                                                                         >  1:336674/4‑149 (MQ=255)
                          cAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAt                                                                                                                         >  2:966048/1‑149 (MQ=255)
                             gACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCTATATGGTCAAAATTGGCTCAAATGcc                                                                                                                      >  2:826035/1‑149 (MQ=255)
                               cAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaa                                                                                                                    >  1:418607/1‑149 (MQ=255)
                                aaCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCGAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCaaaa                                                                                                                   >  2:331268/1‑149 (MQ=255)
                                aaCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACATAATACCTGTATTAACGCGAGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTAATGCCCATTTCGAGGAGATCCTCGATATGGTCAAAATTTGATCAAATGCCaaa                                                                                                                   >  2:764467/1‑149 (MQ=255)
                                 aCTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCaaaa                                                                                                                  <  2:73844/149‑1 (MQ=255)
                                  cTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAAAGCCaaaat                                                                                                                 >  1:211340/1‑148 (MQ=255)
                                   ttGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAaga                                                                                                                >  1:169689/1‑149 (MQ=255)
                                    tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAagag                                                                                                               >  1:269741/1‑149 (MQ=255)
                                    tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAagag                                                                                                               <  1:832328/149‑1 (MQ=255)
                                    tGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAagag                                                                                                               <  2:553460/149‑1 (MQ=255)
                                       gAAACTAATGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCTCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCTATGAGATCCTCGATATGGACAAAATTGTCTCACATGCCAAAAGAGCAt                                                                                                            >  2:581710/1‑149 (MQ=255)
                                        aaaCTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGGCAAAATTGGCTCAAATGCCAAAAGAGCAtt                                                                                                           <  2:492206/149‑1 (MQ=255)
                                          actactGGGTTATTCAGAGTATCTTCACTTTATACCTTTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaa                                                                                                         <  1:358740/149‑1 (MQ=255)
                                            tactGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAATCCTGTGAGATCAGTGGTCACACTATTGATGACCATTTCGAGGAGATCCTCGAGATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTaaaa                                                                                                       >  1:682711/1‑149 (MQ=255)
                                               tGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGAc                                                                                                    >  1:1009868/1‑149 (MQ=255)
                                                gggTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                                   <  2:869524/149‑1 (MQ=255)
                                                   ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTTATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                                <  2:27580/149‑1 (MQ=255)
                                                   ttATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCg                                                                                                >  1:27639/1‑149 (MQ=255)
                                                      ttCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTAc                                                                                             >  2:57722/1‑149 (MQ=255)
                                                       tCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGCTCCTCGATATGGGCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTAct                                                                                            >  2:792164/1‑149 (MQ=255)
                                                         agagTATCGTCACTTTATACCTGTATTAACGCGCGCCTAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGCTCATTAAAAGCCATCTTActct                                                                                          >  2:819619/1‑149 (MQ=255)
                                                          gagTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATTTGGTCAAAATTGTTTCAAATGACAAAAGAGCATTAAAAGACATCGTACtctc                                                                                         >  1:711423/1‑149 (MQ=255)
                                                           agTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTcc                                                                                        >  2:252855/1‑149 (MQ=255)
                                                           agTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTcc                                                                                        <  1:826574/149‑1 (MQ=255)
                                                                 gTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAt                                                                                  <  2:404233/149‑1 (MQ=255)
                                                                     cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                              <  2:179260/149‑1 (MQ=255)
                                                                     cTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCt                                                                              <  2:338348/149‑1 (MQ=255)
                                                                      tttATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTg                                                                             >  2:340417/1‑149 (MQ=255)
                                                                         ataCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAACGACATCGTACTCTCACTCTATGCCTGTTa                                                                          >  2:921737/1‑149 (MQ=255)
                                                                           aCCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAcc                                                                        >  1:876209/1‑149 (MQ=255)
                                                                             cTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTg                                                                      >  2:143205/1‑149 (MQ=255)
                                                                              tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                                   >  1:778400/1‑119 (MQ=255)
                                                                              tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACa                                                                                                   <  2:778400/119‑1 (MQ=255)
                                                                              tGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTgg                                                                     <  1:378868/149‑1 (MQ=255)
                                                                               gTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                                    <  1:262927/149‑1 (MQ=255)
                                                                               gTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgt                                                                    <  2:836692/149‑1 (MQ=255)
                                                                                tATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgta                                                                   <  1:818858/149‑1 (MQ=255)
                                                                                  ttAACGCGCGCCAAAGAAGCCTGGGAGAACAGTTGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCAGgtagt                                                                 <  1:545195/149‑1 (MQ=255)
                                                                                   tAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGgtagta                                                                <  1:532080/149‑1 (MQ=255)
                                                                                    aaCGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAc                                                               <  1:501182/149‑1 (MQ=255)
                                                                                      cgcgcgCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCAAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaa                                                             <  2:767971/149‑1 (MQ=255)
                                                                                       gcgcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACaaa                                                            <  2:253827/149‑1 (MQ=255)
                                                                                         gcgcCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCATCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAAc                                                          >  1:346681/1‑149 (MQ=255)
                                                                                             cAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCg                                                      >  2:419260/1‑149 (MQ=255)
                                                                                              aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGa                                                     <  1:30879/149‑1 (MQ=255)
                                                                                              aaaGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGCACAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATCCCTGTAACCTGGTAGTACAAAACGGCGa                                                     >  1:171444/1‑149 (MQ=255)
                                                                                               aagaagCCTGTGAGAACAGTGGTCACAGAATTGATGACCATTTCTAGGAGATCCTCGATATGGTCAATATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGAc                                                    <  2:538533/149‑1 (MQ=255)
                                                                                                    tccTGTGAGAACAGTGGTCACTCAATTGATGTCTATTTTGAGTAGCTCGTCGATATGGTGATAATTGGCTCAAATGCCAAAAGTGTATTAAAAGACATCGTACTCTCGCGCTATGCCTGTTACCTGGTAGTACAAATCGGCGACCCTGc                                               <  2:500899/148‑1 (MQ=255)
                                                                                                        gtgAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGaaa                                           >  1:32177/1‑149 (MQ=255)
                                                                                                         ggagaACAGGGGTCACACAATTGAAGACCATTTCGATGAGATCCTAGTTATGGTCAAAATTGGCGCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAAc                                          <  1:738246/148‑1 (MQ=255)
                                                                                                          gagaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCTTTAAAAGACATCGTAGTCTCCCTCTATGCGTGTTTCCTGGTAGTACAAAACGGCGACCCTGCGAATcc                                         >  2:195166/1‑149 (MQ=255)
                                                                                                           ataaaaGTGGTCAAACAATTTATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGATCAAATTCCAAAAGAGCATTAAAAGACATCCTACTCTCCCGCTATGCCTGTTAACTGGTAGTACAAAAAGGCGACCCTGCGAAACCg                                        <  2:684630/144‑1 (MQ=255)
                                                                                                           agaACAGTGGTCACACAATTGATGACCATTTAGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTAAGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCg                                        <  1:57722/149‑1 (MQ=255)
                                                                                                            taaCAGTGGTCACACAATTGATGACCATTTCGATGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCgg                                       <  2:205029/148‑1 (MQ=255)
                                                                                                            gaACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCgg                                       <  1:501136/149‑1 (MQ=255)
                                                                                                             aaCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTAGAAAACGGCGACCCTGCGAAACCGGt                                      >  1:911864/1‑149 (MQ=255)
                                                                                                              aCAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTc                                     >  1:413533/1‑149 (MQ=255)
                                                                                                               cAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCa                                    >  1:448328/1‑149 (MQ=255)
                                                                                                                     tCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCgg                              >  1:1001516/1‑149 (MQ=255)
                                                                                                                         acaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATTACTAAAGAGAATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTAGATGGTAGTACAAAACGGCGACCCTGCGCATCCGGTAATTGCggcagg                          >  1:487370/1‑149 (MQ=255)
                                                                                                                         acaATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagg                          >  1:258997/1‑149 (MQ=255)
                                                                                                                            aTTGATGACCATTTCGAGGAGATCCTCGATATGGCCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggca                       <  1:487470/149‑1 (MQ=255)
                                                                                                                            aTTGATGACCATTGCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCggcagggca                       <  2:614645/149‑1 (MQ=255)
                                                                                                                                atgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGTGCAGACt                   >  1:791021/1‑149 (MQ=255)
                                                                                                                                 tgaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAATAGCATTAAAAGACATCGTACTATACCGCTATGCCTGTTACATGGTAGTACAAAACGGCTACCCTGCGATACCGGTCATTGCGGAAGGGCAGAcat                  >  2:851597/1‑147 (MQ=255)
                                                                                                                                 tgaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGCCCCTGCGAATCCGGTCATTGCGGCAGGGCAGACtt                  >  1:407031/1‑149 (MQ=255)
                                                                                                                                  gaCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTa                 >  2:48204/1‑149 (MQ=255)
                                                                                                                                  gaCCATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGTAACCGGTCATTGCGGCAGGGCAGACGTa                 >  1:989504/1‑149 (MQ=255)
                                                                                                                                   aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGCCCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt                >  1:315994/1‑149 (MQ=255)
                                                                                                                                   aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt                >  2:973407/1‑149 (MQ=255)
                                                                                                                                   aCCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAt                >  1:945617/1‑149 (MQ=255)
                                                                                                                                    ccATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAtt               <  1:387120/149‑1 (MQ=255)
                                                                                                                                     cATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAttt              >  2:639379/1‑149 (MQ=255)
                                                                                                                                     cATATCGAGGATCTCCTCGAAATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAttt              >  1:20244/1‑149 (MQ=255)
                                                                                                                                       tttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAATGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTg            <  1:1019759/149‑1 (MQ=255)
                                                                                                                                        ttCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGc           >  1:430970/1‑149 (MQ=255)
                                                                                                                                         tCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTTGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTAttgttga          <  1:254463/149‑6 (MQ=255)
                                                                                                                                           gaggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCAATGCCTGTTAACTGGTCGTACAAAACGGCGACCCTGCGAAACCGGGCATTGCGGCAGGGCAGACTTATTTTGCTAt        <  2:900274/149‑1 (MQ=255)
                                                                                                                                            aggagATCCTCGATATGTTCAAATTTGGCTCAAATGACAAAAGAGGATTATAAGAAATCGTACTCTCCCGCTTTGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATc       <  1:764467/149‑1 (MQ=255)
                                                                                                                                            aggagATCCCCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATc       <  1:872830/149‑1 (MQ=255)
                                                                                                                                             ggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCATGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATcc      <  2:333059/149‑1 (MQ=255)
                                                                                                                                             ggagATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGATACCTGCTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATcc      <  1:355924/149‑1 (MQ=255)
                                                                                                                                                 aTCCTGTATATGGTCAAAATTGGCTCAAATGACAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAc  <  2:658100/149‑1 (MQ=255)
                                                                                                                                                 aTCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAc  >  1:382654/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGCAAACGGAGCAGAACAATGGTCAGCAAGACAACTTGGGAAACTACTGGGTTATTCAGAGTATCGTCACTTTATACCTGTATTAACGCGCGCCAAAGAAGCCTGTGAGAACAGTGGTCACACAATTGATGACCATTTCGAGGAGATCCTCGATATGGTCAAAATTGGCTCAAATGCCAAAAGAGCATTAAAAGACATCGTACTCTCCCGCTATGCCTGTTACCTGGTAGTACAAAACGGCGACCCTGCGAAACCGGTCATTGCGGCAGGGCAGACTTATTTTGCTATCCAGAC  >  NC_000913/3817804‑3818097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: