| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 256,926 | A→G | 5.5% | I134V (ATT→GTT) | frsA → | fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 256,926 | 0 | A | G | 5.5% | 213.3 / 2.9 | 73 | I134V (ATT→GTT) | frsA | fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity |
| Reads supporting (aligned to +/- strand): ref base A (39/30); new base G (2/2); total (41/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01 | |||||||||||
TATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGTGCGCCCATCACCGG > NC_000913/256780‑257074 | tataCGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACAtt > 1:369460/1‑149 (MQ=255) atacgatacgGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTg < 1:24734/149‑1 (MQ=255) acgataccGTGATTGGAGAGCGGGGCGGCAAAGGGGTTCATGAGTGGGCATCGCAGGCGAGTGTGTGGCAACAATTAGCGTGTGCGGAAGACGATCCGCTACTCATTGGTCGTCACTGGCTGCATGCGTCTACGTTGTACAACATTgaa < 2:918337/149‑3 (MQ=255) gatacgGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTtccgc > 1:655037/1‑149 (MQ=255) aacgGTGATTGGCTACCGTGGCGGCAACTGGAGTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCAATGTTGTACAACGTAgccgcat < 1:637493/148‑3 (MQ=255) ggTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATc < 1:752720/149‑1 (MQ=255) ggTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGATGTACAACATTGCCGCCTAta > 2:424222/1‑148 (MQ=255) gttaTTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCAACCAGGCGATGGTGTGGCAACAAAAAGCATGTTAGGAAGACGATCCGCAACTCAGTGGTCTGCACTTACTTCATGCGGCTACGTTGTACAACATTGCCGCCTATca > 2:764061/4‑148 (MQ=255) cTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGa > 1:529288/1‑149 (MQ=255) cTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGa > 1:406476/1‑149 (MQ=255) ggcAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATACGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCAATGTTGTACAACGTAgccgcatg > 1:1020333/1‑125 (MQ=255) ggcAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATACGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCAATGTTGTACAACGTAgccgcatg < 2:1020333/128‑4 (MQ=255) aaCTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCt > 2:270283/1‑149 (MQ=255) aaCTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCt < 1:422448/149‑1 (MQ=255) aCTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTg < 1:33216/149‑1 (MQ=255) tGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAACGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCGGGc > 2:320280/1‑149 (MQ=255) tGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGc > 2:83286/1‑149 (MQ=255) ttATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGc > 2:946248/1‑149 (MQ=255) tGAGTGGGCCACACAGGCGAGGGTGTGTCAACAAATCGCCTGTGCGTAAGACGATCCGCAACGCATTGGTCGTCACTGGCTGCATGCGGCTACGTTGGACAACATTGCCGCCTATCCTCATCTGAAAGTAGATGACCTGGCCGAGCAAg < 1:919874/149‑1 (MQ=255) aGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAgcg > 1:269356/1‑149 (MQ=255) ccACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCt > 1:150510/1‑149 (MQ=255) cACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCtt > 2:843929/1‑149 (MQ=255) aCCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCttt < 1:843929/149‑1 (MQ=255) cccAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTg < 1:300165/149‑1 (MQ=255) aGGCGATGGTGTGGCAACAAAACGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTcc > 1:966222/1‑148 (MQ=255) aGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCa < 2:147041/149‑1 (MQ=255) aGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGATTTGTCa > 2:113051/1‑149 (MQ=255) ggCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCaa < 1:592007/149‑1 (MQ=255) gCGATGGTGAGGCAACAAAAAGCCTGTGCGGAAGACGATCCCCACCTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCGGCATATACTCATCTGAAAGGAGATGAGCTGGCCGAGCAGTAGCAGGCTTTGTCaaa > 1:848394/1‑149 (MQ=255) ggtgGCAACAAAAAGCCGGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGcc < 2:949179/148‑1 (MQ=255) gtgGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCCGGCTTTGTCAAACCGCGCCt > 1:581032/1‑149 (MQ=255) tgGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGATCGTCACTGGCTGCATGCGGCTACGTGGTACAACATTGCCGCCTATCCTCATCTGATAGGCGAAGCCCGGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCtt > 2:748141/1‑148 (MQ=255) gCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATg > 2:877100/1‑149 (MQ=255) acaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAag > 2:378811/1‑149 (MQ=255) acaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAag < 1:115850/149‑1 (MQ=255) aaaaaGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGCCCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAagag > 1:706979/1‑149 (MQ=255) aaaaGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGCTGACCTGGCCGAGCAAGCGCAGGCTTTGTCAACCCGCGCCTATGAAGAgg > 1:671681/1‑149 (MQ=255) aaaaGCCGGTGCGGAAGACGATCCGAAACTCAGTGGTCTTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAgg < 1:597850/149‑1 (MQ=255) aaaGCCGGTGCGGCAGAGGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTAGGTTGTACAACATTGCCGCATATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGc < 1:439445/149‑1 (MQ=255) aaGCATGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGATGTACAACAATGCCGCATATCCTCATCTGAAAGTAGATCACCTGGCCGATCAAGAGAAGGCTTTTTCAACACGAGCATATGAAGAGGcc > 1:254656/1‑149 (MQ=255) ccTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCt < 1:445958/149‑1 (MQ=255) tgCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTCCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGc < 2:39503/149‑1 (MQ=255) gAAGACGATCCGCAACTCAGTGGTCGTCACTGGCGGCATGCGGCTACGTTGTACAACATTGGCGCCTTTCCTCATCTGAAAGGAGATGCCCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCt > 2:190989/1‑149 (MQ=255) aaGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTa > 1:724810/1‑149 (MQ=255) aGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTAc < 2:838852/149‑1 (MQ=255) cGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCgg > 1:92821/1‑149 (MQ=255) gATCCGCAACTCAGGTGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCggg < 1:703517/149‑1 (MQ=255) gATCCGCAACTAAGTTGTCGTCACTGGCTGCATGCGGATACGTTGTACAACATTGCAGCCAATCCTCATCTGAAAGTAGATGAACTGGCCGATCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCggg > 2:1014321/1‑149 (MQ=255) ccGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCAc < 1:877100/149‑1 (MQ=255) cGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGCCCTGGCCGAGCAAGCGCAGGCTTTGTCACACCGCGCCTATGAAGAGGCCGCTCAGCGTCTCCCGGGCACg > 1:805683/1‑149 (MQ=255) cGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAACCCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACg > 2:782253/1‑149 (MQ=255) cGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACg > 2:804313/1‑149 (MQ=255) gCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGa > 1:302779/1‑149 (MQ=255) aaCTCAGTGGTCTTAACTTGATGCATTCGGCTACGTTTTACAACATTGCCGCATATCCTAATATGAAATTAGATTACCTGGACGAGCAAGCGCAGGATTTGTCAAACCGCGCCTCTGAAGAGGCCGCTCAGCGTCTACCGGGCTCGATg < 1:764061/149‑1 (MQ=255) aaCTCAGAGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCAGCTGAAAGGAGATGACCTGGCCTAGCAGGCGCAGGCTTTGTCAAAGCGCGCCTATGAAGAGGCCGCTCAGCGTCTTCCGGGCACGATg > 2:603939/1‑149 (MQ=255) aCTCAGTGGGCGTCACTGCCTGCATGCGGCTACGTGGTACACCATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGTCCGCTCAGCGTCTACCGGGCACGATGc < 2:748266/149‑1 (MQ=255) atCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTTCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGATCAAGCGCAGGATTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCg < 2:559575/148‑1 (MQ=255) gTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAg < 1:376707/149‑1 (MQ=255) tGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGa > 2:170748/1‑149 (MQ=255) cggcACTGGCGGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGa < 2:1015719/146‑1 (MQ=255) gtcACTGGCTGCATGCTGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGATCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAg < 2:274081/149‑1 (MQ=255) gtcACTGGCTGCATGCGGTTACGTTGTACAACATTGCCGCCTATCCTCAACTGAAAGGAGATGTCCTGGCAGAGCAAGAGCAGGCTTTGTCTAACCGCGACTATGATGATGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAg < 2:278124/149‑1 (MQ=255) cACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGtt > 1:535852/1‑149 (MQ=255) gCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCCGCGTCTACCGGGCACGCGGCGGCAGATGGAGTTTCCCg > 1:74225/1‑149 (MQ=255) gCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTAc > 2:429679/1‑149 (MQ=255) ataggCGGCAATGTTGTACAACGTAGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTAc > 1:346366/5‑149 (MQ=255) aTGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTAccc > 2:1020241/1‑149 (MQ=255) aTGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGACAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGAAGCGGCAGATGGAGTTTACCGTAccc > 2:3604/1‑149 (MQ=255) gCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTGTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCcgg < 1:314936/149‑1 (MQ=255) cGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGGCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCcggc < 1:6464/149‑1 (MQ=255) ggCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCcggcg < 1:386088/149‑1 (MQ=255) cTACGTTGTACAACATTCCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGGCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGt < 1:192395/149‑1 (MQ=255) gTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGTgcgc > 2:285635/1‑149 (MQ=255) acaacaTTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGTGCGCCCATc > 2:544870/1‑149 (MQ=255) acaTTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATCCGGCAGATGGAGTTTACCGTACCCGGCGGTGCGCCCATCAcc > 1:945381/1‑149 (MQ=255) caTTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGTGCGCCCATCACCg < 2:529288/149‑1 (MQ=255) caTTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGTGCGCCCATCACCg < 2:406476/149‑1 (MQ=255) aTTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGTGCGCCCATCACCgg > 2:16189/1‑149 (MQ=255) | TATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTTACCGTACCCGGCGGTGCGCCCATCACCGG > NC_000913/256780‑257074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |