| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 475,971 | C→G | 10.0% | intergenic (+20/+11) | ybaY → / ← ybaZ | outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 475,971 | 0 | C | G | 10.0% | 124.2 / 5.8 | 49 | intergenic (+20/+11) | ybaY/ybaZ | outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base G (3/2); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACC > NC_000913/475825‑476117 | aTTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCGACGACTACCGTACCTTCGACTGCACCATCTCCGGTGCATCCGTCTTCGGCCGTGCCCGCTCCGCCGCTATATTAAGAGCCGTTAACCCTCACCGccc > 1:583142/1‑149 (MQ=255)aTTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGCCTTCACCAACGCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGCCGTTAACCCTCTCCGccc > 1:993559/1‑149 (MQ=255) ttGGTGCCGGTAGAGCAAAGCGCCGTTCCGGTTGAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTGCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCg < 2:147604/149‑1 (MQ=255) ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAgg < 1:163815/149‑1 (MQ=255) cGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTCAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg < 1:299277/149‑1 (MQ=255) aCAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtag < 1:897904/149‑1 (MQ=255) aCAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtag < 1:173249/149‑1 (MQ=255) cAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGGCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 2:344517/149‑1 (MQ=255) aaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTc < 1:261516/149‑1 (MQ=255) ccgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCGCCTACGCAATATTTAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCg > 2:776164/1‑149 (MQ=255) gTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:484198/149‑1 (MQ=255) ggTTCAGGCCAGCGGTGGCGCACCGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCCGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCAGCCCGGAGAGGGTTTGTAGTTCCAGCGATAACGc > 1:871434/1‑149 (MQ=255) ggTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCCCCAACTCAGGTGAATCCGTCTTCGGCAGGTCCAGCTCCTCCGCAATATTAAGCGCCGTTAACCCTCTCCGCCCGGAGCGGGTTAGTAGTTCCAGCGATAACGc > 1:192725/1‑149 (MQ=255) ggTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGc > 2:932101/1‑149 (MQ=255) ggCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGTTATCGCTGGaac < 1:381177/149‑2 (MQ=255) gCGGTGGCGCAACGACTAGAGTACCTTCGACTTCCCCAAATCAGGTGAATCCGTCTCCGGCAGTTCCAGCGCCTACGCAATATTACGCGACGTTACCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCGGCAAGTCGAt < 1:736670/149‑1 (MQ=255) cGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCTAtt < 1:432257/149‑1 (MQ=255) cTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGa > 1:108611/1‑149 (MQ=255) ccGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATTACGCTGCAAGTCGATTTGCCCGCTTCCCgctcc > 2:905911/1‑145 (MQ=255) ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAATCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATcaca > 1:832360/1‑149 (MQ=255) aCTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCtt < 2:457104/149‑1 (MQ=255) ttCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCCGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGTGAGGGGTTGTAGTTACAGCGATATCGCTGCAAGTAGATTTGCCAGCGTCCCGATACCATCACACCTTcg > 2:668764/1‑148 (MQ=255) tCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTg < 1:932101/149‑1 (MQ=255) cACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGc < 2:130314/149‑1 (MQ=255) aCCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTGAGTAGTTCCCGCGATAACGCTGCAAGTCGATTTTCCCGCTTCCCGATACCATCACACctgctgac > 1:74657/1‑147 (MQ=255) ccAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCa > 2:5658/1‑149 (MQ=255) cAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAg > 2:154427/1‑149 (MQ=255) aGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGc < 1:511582/149‑1 (MQ=255) aTCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCg < 1:623754/149‑1 (MQ=255) gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa > 1:69925/1‑149 (MQ=255) ggCAGTTCCAGCTGCTACGCAATATTAAGCTACGTTAACCCTCTCCTCCCGGAGTGTGTTAGTAGTTCCAGCGATAACGCTGAAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGAAGCTGt < 1:71323/149‑1 (MQ=255) ggCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGt < 1:430763/149‑1 (MQ=255) aGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTaaa < 1:400191/149‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtcc < 1:136237/149‑1 (MQ=255) cAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccgg < 2:22486/149‑1 (MQ=255) cTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtcc > 2:845672/1‑149 (MQ=255) cTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtcc > 1:37276/1‑149 (MQ=255) ccTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCTTCCCACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtccgg > 2:808365/1‑149 (MQ=255) ccTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtccgg > 1:925600/1‑149 (MQ=255) ccTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtccgg > 2:913501/1‑149 (MQ=255) gCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGc > 2:412211/1‑149 (MQ=255) ctAACCCTCTCCGGGCGGAGGGGGTTAGTAGTTCCAGCGATACCGCTGCAAGTCGATTTGCCCGCTTCCCGATACCCTCACACCTTCTGCCAGTAATGCCTGTCGCTGCCGCTGTAACTCCGGTCCGGTTAGCGAAATTGTGCCGTGGc > 1:133418/2‑149 (MQ=255) tAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCCCACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAATTCCGGTCCGGTTATCGAAATTGTGCCGTGGCg > 2:1004107/1‑149 (MQ=255) aaCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCCATAACGCTGCATGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCAGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGa < 2:558327/149‑1 (MQ=255) aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCAGCCAGTACTGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGGGCCGTGGCGAt < 2:333906/149‑1 (MQ=255) aCCCACTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCGGCTACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGGTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAt > 1:187499/1‑149 (MQ=255) cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTACCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGCCGCTGTATCTCCGGTCCGGTTAGCGAAAATGTGCCGTGGCGAtt > 1:346750/1‑149 (MQ=255) ctctCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCAGATCCCATCACACCTTCTGCCAGTAATCCCTGTCTCTGACGCTGAAAAACCCGTCCGGTTAGCGATCTTGTCCAGAGGCGCTTaa > 1:573001/1‑149 (MQ=255) cGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCGCACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAaccacc < 2:925600/149‑1 (MQ=255) cGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAaccacc > 1:488577/1‑149 (MQ=255) cGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGACGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAaccacc > 2:98695/1‑149 (MQ=255) | ATTGGTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACC > NC_000913/475825‑476117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |