Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 659,205 A→T 7.3% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913659,2050AT 7.3% 183.2 / 4.7 70intergenic (+55/+46)tatE/lipATatABCE protein translocation system subunit/lipoate synthase
Reads supporting (aligned to +/- strand):  ref base A (37/27);  new base T (2/3);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01

AGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCT  >  NC_000913/659057‑659353
                                                                                                                                                    |                                                                                                                                                    
aGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACACGCTTCa                                                                                                                                                      >  2:136134/1‑149 (MQ=255)
   gTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCaaaa                                                                                                                                                   >  1:865282/1‑149 (MQ=255)
    ttCAAGAAGGCGAGGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTATCATAAAGAGTGACGTGGCTAGCACGACGCTCCCTCAAAATCTTGTTAGATAAAAAAAACCCGCTACaaaaa                                                                                                                                                  >  1:87283/1‑149 (MQ=255)
            ggCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttt                                                                                                                                          <  2:551919/149‑1 (MQ=255)
               gATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCGCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGtttttt                                                                                                                                       <  1:788104/149‑1 (MQ=255)
                atgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTTTTGAAGCGGGttttttg                                                                                                                                      <  1:306767/149‑2 (MQ=255)
                atgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTa                                                                                                                                      >  2:234702/1‑149 (MQ=255)
                         gaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATcagacaga                                                                                                                             >  1:360848/1‑149 (MQ=255)
                            gATGCTGCGGCGAAAAAAGGAGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCAGAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCGATAGCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATgt                                                                                                                          >  1:599283/1‑149 (MQ=255)
                                   cggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAat                                                                                                                   >  2:428978/1‑149 (MQ=255)
                                    ggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAACGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTACGTAatt                                                                                                                  <  2:514403/149‑1 (MQ=255)
                                     gcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCGCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAatta                                                                                                                 <  2:649981/149‑1 (MQ=255)
                                       gAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCCATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAagtaagtagt                                                                                                               >  2:740424/1‑147 (MQ=255)
                                           aaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAg                                                                                                           <  1:292272/149‑1 (MQ=255)
                                                 gcAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTAc                                                                                                     <  1:692063/149‑1 (MQ=255)
                                                   aGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACtt                                                                                                   <  1:648381/149‑1 (MQ=255)
                                                     aCGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTaa                                                                                                 >  2:280689/1‑149 (MQ=255)
                                                        ttGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                              <  1:399404/149‑1 (MQ=255)
                                                          gATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGGTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTcc                                                                                            >  1:308440/1‑149 (MQ=255)
                                                           aTCTTCAGTCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                           <  1:239504/149‑1 (MQ=255)
                                                           aTCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                           <  1:243271/149‑1 (MQ=255)
                                                            tCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCAt                                                                                          <  2:459557/149‑1 (MQ=255)
                                                             cTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATc                                                                                         >  2:838542/1‑149 (MQ=255)
                                                              ttCAGGCTGAAAAGCTCTCTCATAAAGAGTGACTTTGCGAGCAGGACGGTGCATCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATAATTACTGGATTACTTAACTTCCATcc                                                                                        <  2:925192/149‑1 (MQ=255)
                                                               tCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACATAAAACCCGCTTCAAAACGCGGGTTATTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACATCCAtaac                                                                                       >  2:568473/1‑146 (MQ=255)
                                                               tCAGGATGAACATCTCGCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCATCAATATCTTGGTCGATACCAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATccc                                                                                       <  2:921775/149‑1 (MQ=255)
                                                                     tGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGc                                                                                 >  1:410437/1‑149 (MQ=255)
                                                                      gAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGcc                                                                                >  1:826501/1‑149 (MQ=255)
                                                                       aaaaGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCt                                                                               >  1:79484/1‑149 (MQ=255)
                                                                               tctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGGTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCg                                                                       <  2:55303/149‑1 (MQ=255)
                                                                                   ttAAACAGTGACGTGGCGAGCATGACGCTCCCTCATTATCTTGTTCGATACTAAAAACCGGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAACTCGGCGt                                                                   <  2:960845/148‑1 (MQ=255)
                                                                                    tAAAGCGTGACGTGGCGAGCAGGACGCTCCCTCAATATCGTGTTCGATACAACAAACCCGCTTCAAAAAGCGGGTTTGTGAGCAGACAGATGTACGTAAGTATTCCAGGATTACGTACCTTCCATCCCTTTCGCCGGCACATCGGCggg                                                                  >  1:932709/1‑147 (MQ=255)
                                                                                    tAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGCTTACTTAACTTCAATCCCTTTCGCCTGCAAATCGGCGTg                                                                  >  1:737452/1‑149 (MQ=255)
                                                                                    tAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCATAACGCGGGTTTTTGATCAGACAGATGTAAGTAATTATTACTGTTTTACTTAACTTCCATCCCTTTCGCCTGCAATTGGGGGTg                                                                  >  2:986806/1‑149 (MQ=255)
                                                                                     aaaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCCACCCTTTCGCCTCCAAATCGGCGTgg                                                                 >  1:550862/1‑149 (MQ=255)
                                                                                      aaGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCCGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCAACCCTTTCGCCTGCAAATCGGCGTGGt                                                                >  1:868172/1‑149 (MQ=255)
                                                                                        gagTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaa                                                              <  2:454722/149‑1 (MQ=255)
                                                                                             aCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAagaaga                                                         >  1:835642/1‑149 (MQ=255)
                                                                                             aCGTCGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATCTAAGAAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGAAagaaga                                                         >  2:887549/1‑149 (MQ=255)
                                                                                              cggggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAagag                                                        <  2:699345/146‑1 (MQ=255)
                                                                                               gTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGc                                                       <  2:435552/149‑1 (MQ=255)
                                                                                                 ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCAATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCgg                                                     >  2:455913/1‑149 (MQ=255)
                                                                                                  gCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGa                                                    <  1:455913/149‑1 (MQ=255)
                                                                                                    gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACa                                                  >  1:592769/1‑149 (MQ=255)
                                                                                                    gAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTGTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGAGGACa                                                  >  1:871292/1‑149 (MQ=255)
                                                                                                        aGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACTGATGTAAGTAATGATTACCGGATTACTTATATTCCCGCCCTTTCGCGGGCACATCGGCGAGGTAATCAGAGCGGACACACg                                              >  2:337887/1‑149 (MQ=255)
                                                                                                          gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                            >  1:41356/1‑149 (MQ=255)
                                                                                                          gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGa                                            <  2:865282/149‑1 (MQ=255)
                                                                                                            cGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGAGAAACGGAcc                                          >  2:197113/1‑149 (MQ=255)
                                                                                                             gCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTAAAGGATTAATTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCTGACAAACGGCCCg                                         >  2:711594/1‑149 (MQ=255)
                                                                                                               tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCa                                       >  2:309044/1‑149 (MQ=255)
                                                                                                                  cTCAATATCTTGTTCGATACAAAAAACCCGCTTCATAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgc                                    >  1:204864/1‑149 (MQ=255)
                                                                                                                     aaTATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAACCGGACCGCATgcagc                                 >  1:817677/1‑149 (MQ=255)
                                                                                                                       tatCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCAt                               <  1:280689/149‑1 (MQ=255)
                                                                                                                        atCTTGTTCGATACAAAAAACCCGCTTCAAACAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATg                              <  2:589427/149‑1 (MQ=255)
                                                                                                                         tattGTTAGATACAAAAAACCCGCTTCAAAAAGCTGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                             <  2:871292/147‑1 (MQ=255)
                                                                                                                         tCTTGGTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                             <  2:249144/149‑1 (MQ=255)
                                                                                                                           ttGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATAAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGt                           >  2:765058/1‑149 (MQ=255)
                                                                                                                              ttCGATACAAAAAACCCGCTTAAAAACGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTaaa                        <  2:692347/149‑1 (MQ=255)
                                                                                                                                  tgataaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                    >  2:277327/5‑149 (MQ=255)
                                                                                                                                  aTACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                    <  2:300281/149‑1 (MQ=255)
                                                                                                                                  aTACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                    >  1:457640/1‑149 (MQ=255)
                                                                                                                                     taaaaaaCCCGCTTTTTGAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCAAGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAATCACATc                 >  2:79923/2‑149 (MQ=255)
                                                                                                                                      aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATTCAGCATGGGTAAAGACCATCg                >  2:996302/1‑149 (MQ=255)
                                                                                                                                      aaaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCg                >  1:776666/1‑149 (MQ=255)
                                                                                                                                       aaaaaCCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGc               >  2:543041/1‑149 (MQ=255)
                                                                                                                                          aaaccGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAg            <  1:234702/146‑1 (MQ=255)
                                                                                                                                           aCCCGCTTCGAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAAATATTACAGGATTACTTAACTTCCATCCCTTTCGCATGCAAATCGGCGTGGTAAGAAGAGCGGACAAACCGACCCCATGCAGCATGGGTAAAGCCCATCGCCCgc           >  1:193999/1‑149 (MQ=255)
                                                                                                                                                cTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTc      >  2:297565/1‑149 (MQ=255)
                                                                                                                                                cTTCAAAAAGCGGGTTTTTTACCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTc      >  2:573612/1‑149 (MQ=255)
                                                                                                                                                 ttCAAAAAGCGTGTTTTTAATCAGATAGATGTAAGTAATTATTTCAGGAGTACTTAAGTTCCACCCCTATCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCCGCATGGGTAAAGCCCATCGCCAGCGCTTCg     <  1:666832/149‑1 (MQ=255)
                                                                                                                                                   cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGc   <  2:1024050/149‑1 (MQ=255)
                                                                                                                                                   cAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGAGCTTCGGc   >  1:458146/1‑149 (MQ=255)
                                                                                                                                                    aaaaaGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCt  >  2:368528/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTTTATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCT  >  NC_000913/659057‑659353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑