Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,141,151 T→C 7.0% intergenic (+165/+31) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,1510TC 7.0% 143.0 / 3.2 61intergenic (+165/+31)flgL/rneflagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base T (30/23);  new base C (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

TCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGACGC  >  NC_000913/1141004‑1141299
                                                                                                                                                   |                                                                                                                                                    
tCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTTGTGCGGGCATTTTTTTGCTTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGtt                                                                                                                                                     <  1:258477/149‑1 (MQ=255)
 cATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTa                                                                                                                                                    >  2:271786/1‑149 (MQ=255)
    gtaaatGGGGATGTTTTGGCGGCCTGCTCTGGGATAGCGTGGGCGGGGATTTTTTTGCCTAATTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                                 <  2:333641/144‑1 (MQ=255)
    gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCGGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGAAGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                                 <  2:106452/149‑1 (MQ=255)
     aaaCTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAg                                                                                                                                                <  1:817958/149‑1 (MQ=255)
        cttggTATGTTTTGTCAGCCTGCTCTGTGATCGCTGGTGCGGGAATTTTTATGCCTATTTTGCATTGTTGGTTAGCAAGGCTGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGc                                                                                                                                             <  2:606647/146‑1 (MQ=255)
           ggTTTGTTTTGTCTGCCTGCTCTGGGCTCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCttt                                                                                                                                          <  2:960300/149‑2 (MQ=255)
             tATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCAGTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAt                                                                                                                                        >  1:968079/1‑149 (MQ=255)
              aTGTTTTGTCTGCCTGCTATGGGATCGCTGGGGCGGGCATTTTTTTTCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTAAAAGATGAAATAAAAAATCCCTGGTAACTGCCAGGGCtttttt                                                                                                                                       <  2:686681/149‑5 (MQ=255)
                gTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATTCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTAc                                                                                                                                     >  1:334504/1‑149 (MQ=255)
                 ttttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACt                                                                                                                                    >  2:984868/1‑149 (MQ=255)
                   ttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTGGGGTAGCAAGGATGCCATTCGATGAATTGTAATATGTGGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTGGATTACttg                                                                                                                                  >  2:823588/1‑148 (MQ=255)
                     gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                                <  2:220393/149‑1 (MQ=255)
                       ctgcctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATGAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGc                                                                                                                              >  1:960658/1‑149 (MQ=255)
                       ctgcctgcTCTGGGAGCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCACGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGc                                                                                                                              <  2:149502/149‑1 (MQ=255)
                              ctaagtgATCGCGGGGGCGGGCATTTTTTTTCCTATTTTGCATTGTTTGTTAGCAAGGAGGCCATTCGATGAATTTTATTATGTTGATTCAAAGATGAAATAATATAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAattat                                                                                                                       <  2:577068/143‑1 (MQ=255)
                                ctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAAttatta                                                                                                                     >  2:820221/1‑149 (MQ=255)
                                  gggATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACt                                                                                                                   <  1:1016244/149‑1 (MQ=255)
                                   ggATCGCTGGGGCGGGCAATTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTAACAGGGCTTGATTAATTTGACCTAATTATTACTc                                                                                                                  >  1:203867/1‑149 (MQ=255)
                                    gATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCa                                                                                                                 >  1:434655/1‑149 (MQ=255)
                                         cTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAgg                                                                                                            >  2:265164/1‑149 (MQ=255)
                                         cTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGATTGATTACTTTGCGCTAATTATTACTCAACAgt                                                                                                            >  1:58558/1‑148 (MQ=255)
                                                ggtcATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATTCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGa                                                                                                     <  1:863208/146‑1 (MQ=255)
                                                   cATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAcgc                                                                                                  <  1:350944/149‑1 (MQ=255)
                                                     tttttttGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACgcgc                                                                                                >  1:461053/1‑149 (MQ=255)
                                                       tttttGCCTATTTTGCATTGATGGTTAGAAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAg                                                                                              <  2:578249/149‑1 (MQ=255)
                                                            gCCTAATTTGCATTGTTGGTTACCAAGGAGGCCATTCGATGAATTTTAATATGTTGATTCAAAGAGGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCg                                                                                         <  1:900547/149‑1 (MQ=255)
                                                             ccTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATATTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCgg                                                                                        <  2:591759/149‑1 (MQ=255)
                                                                 ttttTCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAAAAAAAAAGCCATGAAAGATACCATCGCATGATTACTTTTAGCTAAATATTACTAAACATGTTTCGGACGCACAGTAGCTGCaga                                                                                    >  2:684047/1‑149 (MQ=255)
                                                                 ttttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTTTTTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCaga                                                                                    <  2:491504/149‑1 (MQ=255)
                                                                 ttttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTGGATTACTGTGCGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGAaga                                                                                    >  1:681402/1‑149 (MQ=255)
                                                                   ttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAgg                                                                                  <  1:737525/149‑1 (MQ=255)
                                                                        ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGTAGCTGCAGAGGCatga                                                                             >  1:255137/1‑149 (MQ=255)
                                                                        ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCTGCAGAGGCatga                                                                             >  2:998133/1‑149 (MQ=255)
                                                                        ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCatga                                                                             >  1:27597/1‑149 (MQ=255)
                                                                             ggTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATgtgt                                                                        >  2:168064/1‑149 (MQ=255)
                                                                                   cAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGt                                                                  <  2:528417/149‑1 (MQ=255)
                                                                                      ggATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATg                                                               >  2:788226/1‑149 (MQ=255)
                                                                                         tGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGacc                                                            >  1:585674/1‑149 (MQ=255)
                                                                                           ccATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATACAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGaccac                                                          <  2:988610/149‑1 (MQ=255)
                                                                                               tCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGc                                                      <  1:216291/149‑1 (MQ=255)
                                                                                                cGATGAATTTTAATTTGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGGTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCg                                                     <  1:823588/149‑1 (MQ=255)
                                                                                                cGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGGTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCg                                                     <  2:832949/149‑1 (MQ=255)
                                                                                                 gATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGACGTATGACCACCTGCgg                                                    >  2:860369/1‑149 (MQ=255)
                                                                                                  aTGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGc                                                   >  1:483774/1‑149 (MQ=255)
                                                                                                        tttAATATGTTGATTCAAAGCTGAAATAAAAACGCCGTGGCAGTTACCAGGTCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGTACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCtta                                             >  1:483962/1‑148 (MQ=255)
                                                                                                           aaTATGTTCTTTCAAAGATGAAATAAAAAAGCCCTGGCAGATACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTAcc                                          <  1:295139/149‑1 (MQ=255)
                                                                                                                gTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGa                                     >  1:622601/1‑149 (MQ=255)
                                                                                                                 ttGATTCAAAGATGAAATTATAAAGCCCTTGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCTGACGTGCAGGATCGGCAGAGGCATGATTGGTTGCCGTATGACCACCTGCGTCACCTTTACCTTCGaa                                    <  1:684047/149‑1 (MQ=255)
                                                                                                                 ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTTCTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                    <  2:69467/149‑1 (MQ=255)
                                                                                                                 ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGCCCACCTGCGGCACCTTTACCTGCGaa                                    >  2:828248/1‑149 (MQ=255)
                                                                                                                 ttGATTAAAAGATGAAATAAAAAAGCCATTGCAGTTAACAGGGCTTGATTACTTTGAGCTAATTATTACTCAAGAGGTTGCTGACGCGCAGGAGCGGCAGAGCCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                    <  1:576988/149‑1 (MQ=255)
                                                                                                                  tGATTCATAGATGAAATTAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGATGTATTTATTACTCAACAGGTTGCTGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCAACTGCGTGACGTTTACCTTCGAAg                                   <  2:203867/149‑1 (MQ=255)
                                                                                                                        aaaGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCaaaa                             <  1:265164/149‑1 (MQ=255)
                                                                                                                              gAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAggg                       >  1:238554/1‑149 (MQ=255)
                                                                                                                               aaaTAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGGTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGgc                      >  1:562462/1‑149 (MQ=255)
                                                                                                                                 aTAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACATGCGGCAACTTTACCTTGGAAGGCAAAAGTAGGgcgc                    >  2:757458/1‑149 (MQ=255)
                                                                                                                                  tAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCt                   >  1:231876/1‑149 (MQ=255)
                                                                                                                                  tAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCt                   >  1:140163/1‑149 (MQ=255)
                                                                                                                                   aaaaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCGTTACCTTCGCAGGCAAAAGTAGGGCGCTg                  >  1:947793/1‑149 (MQ=255)
                                                                                                                                         gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTc            >  2:659629/1‑149 (MQ=255)
                                                                                                                                         gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTc            >  2:195871/1‑149 (MQ=255)
                                                                                                                                                   ttACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCCCCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGAcgc  >  2:498359/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGACGC  >  NC_000913/1141004‑1141299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑