Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,141,153 A→G 6.5% intergenic (+167/+29) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,1530AG 6.5% 161.6 / 3.1 61intergenic (+167/+29)flgL/rneflagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base A (31/26);  new base G (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

CATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGACGCGG  >  NC_000913/1141005‑1141301
                                                                                                                                                    |                                                                                                                                                    
cATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTa                                                                                                                                                      >  2:271786/1‑149 (MQ=255)
   gtaaatGGGGATGTTTTGGCGGCCTGCTCTGGGATAGCGTGGGCGGGGATTTTTTTGCCTAATTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                                   <  2:333641/144‑1 (MQ=255)
   gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCGGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGAAGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                                   <  2:106452/149‑1 (MQ=255)
    aaaCTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAg                                                                                                                                                  <  1:817958/149‑1 (MQ=255)
       cttggTATGTTTTGTCAGCCTGCTCTGTGATCGCTGGTGCGGGAATTTTTATGCCTATTTTGCATTGTTGGTTAGCAAGGCTGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGc                                                                                                                                               <  2:606647/146‑1 (MQ=255)
          ggTTTGTTTTGTCTGCCTGCTCTGGGCTCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCttt                                                                                                                                            <  2:960300/149‑2 (MQ=255)
            tATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCAGTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAt                                                                                                                                          >  1:968079/1‑149 (MQ=255)
             aTGTTTTGTCTGCCTGCTATGGGATCGCTGGGGCGGGCATTTTTTTTCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTAAAAGATGAAATAAAAAATCCCTGGTAACTGCCAGGGCtttttt                                                                                                                                         <  2:686681/149‑5 (MQ=255)
               gTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATTCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTAc                                                                                                                                       >  1:334504/1‑149 (MQ=255)
                ttttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACt                                                                                                                                      >  2:984868/1‑149 (MQ=255)
                  ttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTGGGGTAGCAAGGATGCCATTCGATGAATTGTAATATGTGGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTGGATTACttg                                                                                                                                    >  2:823588/1‑148 (MQ=255)
                    gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                                  <  2:220393/149‑1 (MQ=255)
                      ctgcctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATGAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGc                                                                                                                                >  1:960658/1‑149 (MQ=255)
                      ctgcctgcTCTGGGAGCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCACGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGc                                                                                                                                <  2:149502/149‑1 (MQ=255)
                             ctaagtgATCGCGGGGGCGGGCATTTTTTTTCCTATTTTGCATTGTTTGTTAGCAAGGAGGCCATTCGATGAATTTTATTATGTTGATTCAAAGATGAAATAATATAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAattat                                                                                                                         <  2:577068/143‑1 (MQ=255)
                               ctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAAttatta                                                                                                                       >  2:820221/1‑149 (MQ=255)
                                 gggATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACt                                                                                                                     <  1:1016244/149‑1 (MQ=255)
                                  ggATCGCTGGGGCGGGCAATTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTAACAGGGCTTGATTAATTTGACCTAATTATTACTc                                                                                                                    >  1:203867/1‑149 (MQ=255)
                                   gATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCa                                                                                                                   >  1:434655/1‑149 (MQ=255)
                                        cTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAgg                                                                                                              >  2:265164/1‑149 (MQ=255)
                                        cTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGATTGATTACTTTGCGCTAATTATTACTCAACAgt                                                                                                              >  1:58558/1‑148 (MQ=255)
                                               ggtcATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATTCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGa                                                                                                       <  1:863208/146‑1 (MQ=255)
                                                  cATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAcgc                                                                                                    <  1:350944/149‑1 (MQ=255)
                                                    tttttttGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACgcgc                                                                                                  >  1:461053/1‑149 (MQ=255)
                                                      tttttGCCTATTTTGCATTGATGGTTAGAAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAg                                                                                                <  2:578249/149‑1 (MQ=255)
                                                           gCCTAATTTGCATTGTTGGTTACCAAGGAGGCCATTCGATGAATTTTAATATGTTGATTCAAAGAGGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCg                                                                                           <  1:900547/149‑1 (MQ=255)
                                                            ccTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATATTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCgg                                                                                          <  2:591759/149‑1 (MQ=255)
                                                                ttttTCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAAAAAAAAAGCCATGAAAGATACCATCGCATGATTACTTTTAGCTAAATATTACTAAACATGTTTCGGACGCACAGTAGCTGCaga                                                                                      >  2:684047/1‑149 (MQ=255)
                                                                ttttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTTTTTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCaga                                                                                      <  2:491504/149‑1 (MQ=255)
                                                                ttttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTGGATTACTGTGCGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGAaga                                                                                      >  1:681402/1‑149 (MQ=255)
                                                                  ttGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAgg                                                                                    <  1:737525/149‑1 (MQ=255)
                                                                       ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGTAGCTGCAGAGGCatga                                                                               >  1:255137/1‑149 (MQ=255)
                                                                       ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCTGCAGAGGCatga                                                                               >  2:998133/1‑149 (MQ=255)
                                                                       ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCatga                                                                               >  1:27597/1‑149 (MQ=255)
                                                                            ggTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATgtgt                                                                          >  2:168064/1‑149 (MQ=255)
                                                                                  cAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGt                                                                    <  2:528417/149‑1 (MQ=255)
                                                                                     ggATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATg                                                                 >  2:788226/1‑149 (MQ=255)
                                                                                        tGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGacc                                                              >  1:585674/1‑149 (MQ=255)
                                                                                          ccATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATACAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGaccac                                                            <  2:988610/149‑1 (MQ=255)
                                                                                              tCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGc                                                        <  1:216291/149‑1 (MQ=255)
                                                                                               cGATGAATTTTAATTTGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGGTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCg                                                       <  1:823588/149‑1 (MQ=255)
                                                                                               cGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGGTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCg                                                       <  2:832949/149‑1 (MQ=255)
                                                                                                gATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGACGTATGACCACCTGCgg                                                      >  2:860369/1‑149 (MQ=255)
                                                                                                 aTGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGc                                                     >  1:483774/1‑149 (MQ=255)
                                                                                                       tttAATATGTTGATTCAAAGCTGAAATAAAAACGCCGTGGCAGTTACCAGGTCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGTACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCtta                                               >  1:483962/1‑148 (MQ=255)
                                                                                                          aaTATGTTCTTTCAAAGATGAAATAAAAAAGCCCTGGCAGATACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTAcc                                            <  1:295139/149‑1 (MQ=255)
                                                                                                               gTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGa                                       >  1:622601/1‑149 (MQ=255)
                                                                                                                ttGATTCAAAGATGAAATTATAAAGCCCTTGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCTGACGTGCAGGATCGGCAGAGGCATGATTGGTTGCCGTATGACCACCTGCGTCACCTTTACCTTCGaa                                      <  1:684047/149‑1 (MQ=255)
                                                                                                                ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTTCTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                      <  2:69467/149‑1 (MQ=255)
                                                                                                                ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGCCCACCTGCGGCACCTTTACCTGCGaa                                      >  2:828248/1‑149 (MQ=255)
                                                                                                                ttGATTAAAAGATGAAATAAAAAAGCCATTGCAGTTAACAGGGCTTGATTACTTTGAGCTAATTATTACTCAAGAGGTTGCTGACGCGCAGGAGCGGCAGAGCCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                      <  1:576988/149‑1 (MQ=255)
                                                                                                                 tGATTCATAGATGAAATTAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGATGTATTTATTACTCAACAGGTTGCTGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCAACTGCGTGACGTTTACCTTCGAAg                                     <  2:203867/149‑1 (MQ=255)
                                                                                                                       aaaGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCaaaa                               <  1:265164/149‑1 (MQ=255)
                                                                                                                             gAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAggg                         >  1:238554/1‑149 (MQ=255)
                                                                                                                              aaaTAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGGTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGgc                        >  1:562462/1‑149 (MQ=255)
                                                                                                                                aTAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACATGCGGCAACTTTACCTTGGAAGGCAAAAGTAGGgcgc                      >  2:757458/1‑149 (MQ=255)
                                                                                                                                 tAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCt                     >  1:231876/1‑149 (MQ=255)
                                                                                                                                 tAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCt                     >  1:140163/1‑149 (MQ=255)
                                                                                                                                  aaaaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCGTTACCTTCGCAGGCAAAAGTAGGGCGCTg                    >  1:947793/1‑149 (MQ=255)
                                                                                                                                        gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTc              >  2:659629/1‑149 (MQ=255)
                                                                                                                                        gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTc              >  2:195871/1‑149 (MQ=255)
                                                                                                                                                  ttACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCCCCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGAcgc    >  2:498359/1‑149 (MQ=255)
                                                                                                                                                    aCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGACGCgg  >  2:738169/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGACGCGG  >  NC_000913/1141005‑1141301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑