Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,449,804 C→T 9.0% A516T (GCT→ACT) ‡ tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,8040CT 9.0% 148.7 / 5.7 57A516T (GCT→ACT) ‡tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base T (3/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

GATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATAC  >  NC_000913/1449660‑1449949
                                                                                                                                                |                                                                                                                                                 
gATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCg                                                                                                                                               <  2:869341/149‑1 (MQ=255)
    gAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTGATCTTTCGCCGTCTCATCGTGCAGGGTTTTCGCTTTTACACCTTTCACCGCTTCGATGCCCGTAGAACCGGCAt                                                                                                                                           >  1:583391/1‑149 (MQ=255)
      ggCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCg                                                                                                                                         <  1:597784/149‑1 (MQ=255)
       gCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGa                                                                                                                                        <  1:246583/149‑1 (MQ=255)
                ataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCCTCTTTCGCCGGCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                               >  2:977535/1‑149 (MQ=255)
                ataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTAGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                               >  1:255927/1‑149 (MQ=255)
                   aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGTTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGAATCGATGCCAATAGTGc                                                                                                                            >  1:322107/1‑149 (MQ=255)
                       tgtgtgtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCAGGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGtt                                                                                                                        <  1:709572/144‑1 (MQ=255)
                          tGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTCGCGCGGGTCATCTTTCGCCGTCTCATCGTGCTTGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTc                                                                                                                     <  1:493177/149‑1 (MQ=255)
                            gtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGACGTTTTCAt                                                                                                                   >  2:37243/1‑149 (MQ=255)
                               tgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGa                                                                                                                >  2:773969/1‑149 (MQ=255)
                                   gtaCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGAt                                                                                                            >  2:89891/1‑149 (MQ=255)
                                      cccACGATATTGTTATCGATACGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGATTTCGCTTTAACAACTTTCACCGCTTCGATTCCCGTAGAACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                         <  1:836596/149‑1 (MQ=255)
                                      cccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                         >  2:73499/1‑149 (MQ=255)
                                      cccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTATAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                         >  2:876986/1‑149 (MQ=255)
                                       ccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAg                                                                                                        <  2:990656/149‑1 (MQ=255)
                                        cACGATTTTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCCTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGt                                                                                                       <  1:361611/149‑1 (MQ=255)
                                        cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTACCACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGt                                                                                                       >  1:987179/1‑149 (MQ=255)
                                        cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCTTCGATGCCAATAGGGCCGTTTTCATGGAAGATCCAGt                                                                                                       >  2:116923/1‑149 (MQ=255)
                                         aCGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGACATCTTTCGCCGTCGCAGCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCCCCGGCATCGATGCCAATAGTGACGTTTTAATGGAAGATcaattc                                                                                                      >  1:979160/1‑144 (MQ=255)
                                                   gATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAg                                                                                            <  1:952213/149‑1 (MQ=255)
                                                    aTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt                                                                                           >  1:710428/1‑149 (MQ=255)
                                                     tCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCCTCGTGCATGGTTTTCGCTTTAACACCTTTCACCGGTTCGATGCCCGTAGCACCGGCATCGATGGCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTc                                                                                          <  1:936917/149‑1 (MQ=255)
                                                                  cGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCAcg                                                                             >  1:141272/1‑148 (MQ=255)
                                                                       cgcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACt                                                                        <  1:119153/149‑1 (MQ=255)
                                                                         cgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGa                                                                      <  2:88696/149‑1 (MQ=255)
                                                                             tCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGGCAAAAATGTAGTCACAGTTACCCACTGTACTGATccc                                                                  >  2:234359/1‑148 (MQ=255)
                                                                              cATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAg                                                                 <  1:73499/149‑1 (MQ=255)
                                                                               aTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgc                                                                <  1:338906/149‑1 (MQ=255)
                                                                               aTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCAAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAGTGATCCAgc                                                                >  2:349683/1‑149 (MQ=255)
                                                                               aTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGAAGCCCGTAGCACCGGCAGCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGGAGTCATAGTTACCCACTGTACTGATCCAgc                                                                <  1:206367/149‑1 (MQ=255)
                                                                               aTCTTTCGCCGTCTCATCGAGCATGGTTTTCGCTTTAACACCTTACACCGCTTCTATGCCCGTAGCCCCGGCATCGATGCCAATAGTGACGTTGTCATGGAAGATACAGTCTAAAATGTAGTCATAGTTACCCACAGATCTGATCCAgc                                                                >  1:294715/1‑149 (MQ=255)
                                                                                 cTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGCTGCCAATAGTGCGGTTTTCATGGAAGATCCAGTCAAAAATGTAGACATAGTTACCCACTGTACTGATCCAgcgc                                                              >  2:713444/1‑149 (MQ=255)
                                                                                 cTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgcgc                                                              <  1:37243/149‑1 (MQ=255)
                                                                                  tttCGCCGTCTCATCGTGCAGGGATATCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGca                                                             <  2:853894/149‑1 (MQ=255)
                                                                                   ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcac                                                            <  2:652368/149‑1 (MQ=255)
                                                                                     cGCCGTATCATCGTTCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacca                                                          <  1:321789/149‑1 (MQ=255)
                                                                                                    cATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCGCGTAGCACCGGCATCGATGCCAATTGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAATGCTCCAGCGCACCACTAACTCCCGGCGtt                                           >  1:280521/1‑149 (MQ=255)
                                                                                                      tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCg                                         >  2:182393/1‑149 (MQ=255)
                                                                                                      tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCg                                         <  1:471768/149‑1 (MQ=255)
                                                                                                         ttttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGCTGCCAATAGTGCCGTTTTCAAGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACAGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTa                                      >  2:322295/1‑149 (MQ=255)
                                                                                                            tCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTg                                   >  2:797578/1‑149 (MQ=255)
                                                                                                              gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc                                 <  1:952682/149‑1 (MQ=255)
                                                                                                              gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCAGTTTTCATGGAAGATCCAGTCAAAATTGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACGAACTCCCGGCGGTCGGTACTGAc                                 >  2:425675/1‑149 (MQ=255)
                                                                                                                   aaCACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTgg                            >  2:659789/1‑149 (MQ=255)
                                                                                                                        cTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTgg                       >  2:449415/1‑149 (MQ=255)
                                                                                                                         tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTGCAGGCCTTCGGTACTGACTTTGGGCTGGc                      >  2:200231/1‑149 (MQ=255)
                                                                                                                         tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc                                 <  1:872038/138‑1 (MQ=255)
                                                                                                                         tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc                                 >  2:872038/1‑138 (MQ=255)
                                                                                                                                 cTTCCATGCCCGTAGCACCTGCATCGATGCCAATCTTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTATTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc              <  1:208644/149‑1 (MQ=255)
                                                                                                                                  atCGATGCCGGTGCTACGGGCATCGCTGCCAATAGTGGCTTTTTCATGGAAGATCCAGTCAAAAATGTAGACAGAGTACCCCACTGTACTTAACCAGCGCACCACAACCACCCGGCCGTCCCTACAGACTATGGGCTGGCCCATTTCCt             >  1:569575/2‑149 (MQ=255)
                                                                                                                                  atCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt             >  2:394626/2‑149 (MQ=255)
                                                                                                                                  atCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt             >  1:895513/2‑149 (MQ=255)
                                                                                                                                   tCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACAAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTg            <  2:858607/149‑1 (MQ=255)
                                                                                                                                      aTGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTGATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATg         <  2:500008/149‑1 (MQ=255)
                                                                                                                                          ccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta     <  1:660345/149‑1 (MQ=255)
                                                                                                                                             tAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATAc  <  1:773969/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATAC  >  NC_000913/1449660‑1449949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑