Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,575,221 T→C 5.4% intergenic (‑19/+26) pqqL ← / ← yddB putative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,575,2210TC 5.4% 200.3 / 2.9 76intergenic (‑19/+26)pqqL/yddBputative periplasmic M16 family zinc metalloendopeptidase/putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base T (30/41);  new base C (2/2);  total (32/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01

GCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATC  >  NC_000913/1575074‑1575367
                                                                                                                                                   |                                                                                                                                                  
gCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAAGCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGCGCGAATTTa                                                                                                                                                   <  1:931902/149‑1 (MQ=255)
 ccATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGAGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTAc                                                                                                                                                  <  1:836837/149‑1 (MQ=255)
  cATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATTATTATCTCCATGCGAAAACCGGGCGAATTTAcc                                                                                                                                                 >  1:824150/1‑149 (MQ=255)
  cATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTAcc                                                                                                                                                 >  1:385979/1‑149 (MQ=255)
   aTTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAAGCAGCCGCCCCGGGAGCAACAGAGTTGCCACTAACGTCCGTAAGAAACAGCGGATTCTCATAAGTATCACCATGCGAAAACCGGGCGAAGTTAccc                                                                                                                                                >  2:997411/1‑149 (MQ=255)
        ccAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGTAAATTCGCCCGGttt                                                                                                                                           >  1:734646/1‑148 (MQ=255)
         cAGTTGCCCGTTAATTAACTTTTCATACTGCGGTAAGGCGGCGGCAATAAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGTAAATTCGCCCGGtttt                                                                                                                                          <  2:371406/149‑3 (MQ=255)
         cAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTaa                                                                                                                                          <  2:830732/149‑1 (MQ=255)
          aGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCACTCAGCCGCCCGGGGCGCAACAGAGTTGCCACTAACGTCAGTAAGAAAAAGAGGTTTCTCATAATTATCTCCTTGCGAAAACCTGGCGAATTTACCCGGTTAAg                                                                                                                                         >  2:226182/1‑149 (MQ=255)
             tGCCCGGTAATTAACTTTTCATCCTGCGGTACGGCGGCGGCAATCAGCCGCACGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGTAAATTCGCCCGGttttcgca                                                                                                                                      <  1:52622/149‑7 (MQ=255)
              gCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGACACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTaaa                                                                                                                                     >  1:644123/1‑149 (MQ=255)
              gCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTATCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTaaa                                                                                                                                     >  1:644126/1‑149 (MQ=255)
              gCCCGCTAATTAACTTTTCATCCAGCGGTAAGGCGGCGGCAATCAGCCTCCCGTGTAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTaaa                                                                                                                                     <  1:338366/149‑1 (MQ=255)
                 cGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATc                                                                                                                                  <  2:329484/149‑1 (MQ=255)
                  ggTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATcc                                                                                                                                 <  1:73532/149‑1 (MQ=255)
                     aaTTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGaa                                                                                                                              <  1:403148/149‑1 (MQ=255)
                      tttAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGACGCCCGTGGAGCAACAGAGTTGCCACTAACGTCAGTTAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAAc                                                                                                                             <  1:578276/148‑1 (MQ=255)
                        tAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTa                                                                                                                           >  1:350705/1‑149 (MQ=255)
                           cTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTa                                                                                                                        >  1:460772/1‑149 (MQ=255)
                                cATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAAtt                                                                                                                   <  2:433871/149‑1 (MQ=255)
                                  tCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTc                                                                                                                 <  1:176760/149‑1 (MQ=255)
                                   ccTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCTGGCGAATTTACCCGGTTAAGTAAAATCAGAACTATTAAAATTTCa                                                                                                                >  2:708146/1‑149 (MQ=255)
                                   ccTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCa                                                                                                                >  1:370598/1‑149 (MQ=255)
                                   ccTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCa                                                                                                                >  1:462330/1‑149 (MQ=255)
                                      gCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGc                                                                                                             <  1:119020/149‑1 (MQ=255)
                                         ttAAGGCGGCGGCAATCAGCCGCCAGGGGAGCAACAGATTTGCCACTAACGTGAGTAAGAAACAGAGTTTTCTCATAATTATCTCCATGCGAAAACCGGGCGATTTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGa                                                                                                          <  1:38176/148‑1 (MQ=255)
                                          tAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGAc                                                                                                         >  2:809096/1‑149 (MQ=255)
                                                ggcggcAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAg                                                                                                   <  1:897588/149‑1 (MQ=255)
                                                 gcggcAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGc                                                                                                  <  2:999548/149‑1 (MQ=255)
                                                      aaTCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGaa                                                                                             >  1:424850/1‑149 (MQ=255)
                                                        tCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAg                                                                                           <  2:629342/149‑1 (MQ=255)
                                                           gccgccCGGGTAGCAACAGAGTTGCCACTAACGTCTGTAAGAAACAGAGGTTTCTCATATTTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTAc                                                                                        >  2:324019/1‑149 (MQ=255)
                                                            ccgccCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTATAATTTCATGCTGACATCAAGCCAGAAAGTACg                                                                                       >  1:690596/1‑149 (MQ=255)
                                                              gccCTGGGAGCAACATAGTTGCCTCTAACGTCAGTAATAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCAGAAATATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACTGc                                                                                     >  2:1004919/1‑149 (MQ=255)
                                                              gccCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGc                                                                                     <  2:248787/149‑1 (MQ=255)
                                                              gccCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGc                                                                                     <  2:126790/149‑1 (MQ=255)
                                                                         aaCAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAg                                                                          <  2:118465/149‑1 (MQ=255)
                                                                         aaCAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTAGCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAATATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAg                                                                          >  1:692095/1‑149 (MQ=255)
                                                                           cAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGATCTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGt                                                                        >  2:130748/1‑149 (MQ=255)
                                                                           cAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTAACCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGt                                                                        >  1:722721/1‑149 (MQ=255)
                                                                               gTTGCCACTAACGTCCGTAAGAAACAGACGTTTCTCATAATTATCTCCATGCGTAAACCGTGCGAATATACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGcc                                                                    <  1:712728/149‑1 (MQ=255)
                                                                                ttGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGccc                                                                   >  2:713243/1‑148 (MQ=255)
                                                                                     aCTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGAACGGCCACTGGCGTAGGTCGCCACACCg                                                              >  1:94520/1‑149 (MQ=255)
                                                                                      cTAACGTCACAAAGAAACAGAGGTTTCTCATAATAATCACCAAGCAAAAACCGCGCTAATTTACCCGGTTAAGTAAAATCAAAACTAATAAAATATCATTATAAAATCAAACCAGAAAGTACGCACACTCGAGTAGATCGCcaccactt                                                             >  2:1002455/1‑144 (MQ=255)
                                                                                       tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGgt                                                            <  2:46803/149‑1 (MQ=255)
                                                                                       tAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGgt                                                            <  1:809096/149‑1 (MQ=255)
                                                                                       aaaCGCCAGTAAGAAACAGAGGTTTCTCATAATTATCACCCCGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGgt                                                            <  1:1028264/148‑1 (MQ=255)
                                                                                         aCGTCAGTAAGAAACAGAGGTTTCTCAAAATTATCTCCATGCGAAAACCGGGCGAATATACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGgtgt                                                          >  2:845619/1‑149 (MQ=255)
                                                                                           gTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAACTACGGCCACTGTCGTAGGTCGCAACACCGgtgtaa                                                        >  2:94379/1‑147 (MQ=255)
                                                                                            gaaGTAAGAAACAGAGGTCTCTCATAATAATCTCCATGCTAAAACCGTGCGAATTAACCCGGATAAGTAAAATCCGAACTATTAAAATTTCCTGCTGACATCAAGCCCGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCg                                                       <  1:836566/147‑1 (MQ=255)
                                                                                               gTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTg                                                    <  2:855953/149‑1 (MQ=255)
                                                                                               gTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTAGTTg                                                    >  1:850082/1‑149 (MQ=255)
                                                                                                tAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAATATCCGAACTATTAAAATTTCATGCTGACATCATGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGt                                                   <  1:461960/149‑1 (MQ=255)
                                                                                                 aaGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTa                                                  >  1:88888/1‑149 (MQ=255)
                                                                                                   gAAACAGAGGTTTCTCATAATTATCTCCATGCGAATACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATc                                                <  1:708146/149‑1 (MQ=255)
                                                                                                   gAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATc                                                <  2:765702/149‑1 (MQ=255)
                                                                                                     aaCAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCaa                                              <  2:482441/149‑1 (MQ=255)
                                                                                                        agagGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCACCAg                                           >  1:821004/1‑149 (MQ=255)
                                                                                                          tgttttCTCATAATAATCTCCATGCGAAAACCGGCCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGTCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCg                                         <  2:187893/146‑1 (MQ=255)
                                                                                                                  cATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCt                                 <  2:644123/149‑1 (MQ=255)
                                                                                                                  cATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCt                                 <  2:644126/149‑1 (MQ=255)
                                                                                                                     aaTTATCTCCATTAGAAAACCGGGCGAATTTAACAGGTTAAGTCAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACTGCAACTGGAGTATGTCGACACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATc                              <  2:488299/149‑1 (MQ=255)
                                                                                                                     aaTTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATc                              <  2:462330/149‑1 (MQ=255)
                                                                                                                     aaTTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATc                              <  1:228946/149‑1 (MQ=255)
                                                                                                                         aTCTCCATGCGAAAACCGGGCGAATTTACCTGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACTGACACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGt                          <  1:863372/149‑1 (MQ=255)
                                                                                                                          tctcCATGCGAAAACCGTGCGAATTTACCCGGGTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTa                         <  2:633388/149‑1 (MQ=255)
                                                                                                                               tttacttaaCCGGGTAAATTCGCCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTGTCCAGTACATTg                    >  1:948210/8‑149 (MQ=255)
                                                                                                                               aTGCGAAATCCGGGCGAATTTACCCGGTTAAGTAATATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTg                    <  1:997411/149‑1 (MQ=255)
                                                                                                                                 gCGAATACGGGGCGAATTTTACTGTTTAAGTAAAATCCGACATATTAAAATTTCATGCTGACATCATGCCAGAAAGTACGGGCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTTTGCCGTACATTgag                  <  1:496965/149‑1 (MQ=255)
                                                                                                                                 gCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGTCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTgag                  <  2:139919/149‑1 (MQ=255)
                                                                                                                                   gAAAACCGGGCGAATTTACCCGGTTAAGTACACTCCGAACTATTAAATTTTCATGCTGACATCAATCCAGAAAGTACGGCAACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACATCGGTTTTGCTATCCAGATCATTGAGaa                >  1:739857/1‑149 (MQ=255)
                                                                                                                                   gAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGACCTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTAGCAACAGAGGTTTTGCTATCCAGTACATTGAGaa                >  1:567656/1‑149 (MQ=255)
                                                                                                                                   gAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGaa                >  1:743283/1‑149 (MQ=255)
                                                                                                                                      aaCCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAatat             <  2:690596/149‑1 (MQ=255)
                                                                                                                                        ccGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCg           <  1:182963/149‑1 (MQ=255)
                                                                                                                                         cGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCgg          <  2:75937/149‑1 (MQ=255)
                                                                                                                                                 tttACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATc  <  2:370598/149‑1 (MQ=255)
                                                                                                                                                 tttACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCAAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTCCATTGAGAATATCGGCACTGagc  >  1:978366/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGTTGCCACTAACGTCAGTAAGAAACAGAGGTTTCTCATAATTATCTCCATGCGAAAACCGGGCGAATTTACCCGGTTAAGTAAAATCCGAACTATTAAAATTTCATGCTGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATC  >  NC_000913/1575074‑1575367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: