Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,704,723 T→C 5.8% intergenic (+47/‑226) blr → / → cnu beta‑lactam resistance membrane protein; divisome‑associated protein/nucleoid‑associated oriC‑binding protein; H‑NS and StpA stabilizing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,704,7230TC 5.8% 192.4 / 2.9 69intergenic (+47/‑226)blr/cnubeta‑lactam resistance membrane protein; divisome‑associated protein/nucleoid‑associated oriC‑binding protein; H‑NS and StpA stabilizing factor
Reads supporting (aligned to +/- strand):  ref base T (24/40);  new base C (2/2);  total (27/42)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

GGTTGGATCGTTCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAAC  >  NC_000913/1704575‑1704868
                                                                                                                                                    |                                                                                                                                                 
ggTTGGATCGTTCTTGTCGTTTCAGTCCTTCTTCATGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGtttt                                                                                                                                                   <  2:87801/149‑1 (MQ=255)
 gTTGGATCGTTCGTGTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGACCAGAGTGCTTAGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTc                                                                                                                                                  >  2:722367/1‑149 (MQ=255)
  ttGGATCGTTCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCa                                                                                                                                                 <  1:511891/149‑1 (MQ=255)
  gtGGATCGTTCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACCACTCTCAGCCACCTGAACAGAGTGCTGCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCa                                                                                                                                                 <  1:739017/148‑1 (MQ=255)
     gATCGTTCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACATCTATCAGCCACCTGAACAGAGTGCTTCGGTACAACAGAAGTTAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACtt                                                                                                                                              <  1:101351/149‑1 (MQ=255)
         gTTCTTGTCGTTGCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGCCAACTATCAGCGACCTGAACAGAGGGCTTCGGTACACCACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCg                                                                                                                                          <  1:869817/149‑1 (MQ=255)
          ttCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCgg                                                                                                                                         >  1:912977/1‑149 (MQ=255)
               gTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATAtt                                                                                                                                    <  1:198718/149‑1 (MQ=255)
                  gTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTCCACTTGTCGGATATTaaa                                                                                                                                 >  1:845207/1‑149 (MQ=255)
                  gTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTCCACTTGTCGGATATTaaa                                                                                                                                 >  1:849408/1‑149 (MQ=255)
                      cAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAg                                                                                                                             <  1:665243/149‑1 (MQ=255)
                         tCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGaa                                                                                                                          >  2:182611/1‑149 (MQ=255)
                         tCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCAGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGaa                                                                                                                          >  1:73677/1‑149 (MQ=255)
                             tcttctTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACCGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATtta                                                                                                                      <  2:182823/149‑1 (MQ=255)
                                  ttGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATc                                                                                                                 <  1:39981/149‑1 (MQ=255)
                                   tggcgtggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCt                                                                                                                >  1:972924/1‑149 (MQ=255)
                                    ggcgtggcgAGGCACATTGACAACTAGCATCCACCTGACCCGAGTGCTTCGGTACAACACTAGTCACCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCCGGAATTTATTATCtt                                                                                                               <  2:259258/149‑1 (MQ=255)
                                    ggcggggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCtt                                                                                                               <  2:377096/149‑1 (MQ=255)
                                       gtggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTTTGCACTTGTGGAGCGACATGCAGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGtt                                                                                                            <  1:498381/149‑1 (MQ=255)
                                        tggcgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTc                                                                                                           <  1:172964/149‑1 (MQ=255)
                                           cgAGTCACATTGACAACTATCATCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAt                                                                                                        <  2:138388/149‑1 (MQ=255)
                                           cgAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAt                                                                                                        <  1:975566/149‑1 (MQ=255)
                                            gAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGAtg                                                                                                       <  1:1010329/149‑1 (MQ=255)
                                                  caTTGACAACTATCAGCCCCCTGAACAGAGGGCGTCGGTACAACACCAGTACGCTCTGCACTTGTGGCGCGCCATGCTGCCCGACCGGGTGCCGGTGTGCCCTTGTCGGATCTTAAACCAGGAATTTATTATCTTGGTCGAGGttgatg                                                                                                 >  2:690034/1‑149 (MQ=255)
                                                  caTTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGttgttg                                                                                                 <  2:158892/149‑1 (MQ=255)
                                                  aaTTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGttgttg                                                                                                 <  1:1007707/148‑1 (MQ=255)
                                                   aTTGCCAACTATCAGCCTCCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCTCTAGTCGGTTATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTgg                                                                                                <  1:685288/149‑1 (MQ=255)
                                                       aCAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTAGGGGAGCGACATGCTGCCCGTCGGGGGGCATGTTTTCACTTGTCGGCGATGAAACCAGGAAGTTAGTATCTTGGACGATggtgatggtgat                                                                                            >  2:217949/1‑149 (MQ=255)
                                                        cAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGAtt                                                                                           <  1:67550/149‑1 (MQ=255)
                                                         aaCTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTg                                                                                          <  2:118560/149‑1 (MQ=255)
                                                          aCTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGt                                                                                         <  1:239586/149‑1 (MQ=255)
                                                            tATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCa                                                                                       >  2:150134/1‑149 (MQ=255)
                                                             aTCAGCAACCTGAACAGAGTTCTTCGGTAGAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGAATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAg                                                                                      <  2:521928/149‑1 (MQ=255)
                                                                tgCCACCTGTACAGAGTGCTTCGGTACAACACTAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACGAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAggca                                                                                   <  2:392748/148‑3 (MQ=255)
                                                                 gCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGGGATTGTCAGGGAt                                                                                  >  1:577769/1‑149 (MQ=255)
                                                                         aaCAGAGTGCTTCGGTACAACACAAGTAATCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGGTTGTGATTGTCAGGGATAGTAAAgc                                                                          <  1:632998/149‑1 (MQ=255)
                                                                                   ttCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAg                                                                                      <  2:235814/127‑1 (MQ=255)
                                                                                   ttCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAg                                                                                      >  1:235814/1‑127 (MQ=255)
                                                                                   ttCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTActct                                                                >  1:583736/1‑149 (MQ=255)
                                                                                     cGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCttt                                                              >  2:126513/1‑149 (MQ=255)
                                                                                       gTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTc                                                            <  1:998248/149‑1 (MQ=255)
                                                                                         acaacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCTGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTGCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGGTTCTCTTTTCag                                                          <  2:599250/149‑1 (MQ=255)
                                                                                         acaacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGGCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCag                                                          >  2:745459/1‑149 (MQ=255)
                                                                                          caacaaAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCAGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTTTTATGTTGTTCGATGTTGTTGGTGAGTGTCAGGGATTGTAATGCGCGTTAATCTTTTCaga                                                         >  2:590298/1‑149 (MQ=255)
                                                                                           aacaCAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCagag                                                        <  1:748780/149‑1 (MQ=255)
                                                                                            acacAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGc                                                       >  2:849123/1‑149 (MQ=255)
                                                                                                aaGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTGGGATATTAACCCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATATAAAAGCGCGTTACTCTTTTCAGAGCACtt                                                   >  2:682556/1‑149 (MQ=255)
                                                                                                 cgTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCTCTTGTCGGATATTAAACCAGGAATTTTTTATCTTGTTCGATGGTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGttt                                                  <  1:314273/148‑1 (MQ=255)
                                                                                                  gTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGtttt                                                 <  1:926506/149‑1 (MQ=255)
                                                                                                        tctGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATcc                                           >  1:367368/1‑149 (MQ=255)
                                                                                                        tctGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTATTTCATcc                                           >  1:655756/1‑149 (MQ=255)
                                                                                                          tGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATACCt                                         >  1:657826/1‑149 (MQ=255)
                                                                                                            cACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGa                                       >  1:456814/1‑149 (MQ=255)
                                                                                                             aCTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGaa                                      <  1:337063/149‑1 (MQ=255)
                                                                                                                   ggAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTg                                <  2:912977/149‑1 (MQ=255)
                                                                                                                   ggAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGGTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTg                                <  1:805942/149‑1 (MQ=255)
                                                                                                                        gACATTCTGCCCGTCCGGGTGCATGTTTTCACTTTTCGGATATTAAACCAGGAATTTATTATCTTGTTCTATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATc                           <  1:25524/149‑1 (MQ=255)
                                                                                                                         aCATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGTGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCa                          <  1:461759/149‑1 (MQ=255)
                                                                                                                          cATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCTGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCAACac                         >  1:374692/1‑148 (MQ=255)
                                                                                                                             gctgcCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATGTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAACTCTCGTTAATATTTTCAGAGCAGTTTTTCATCCGTGAATATTTGCCATCAgcgc                      >  1:566091/1‑149 (MQ=255)
                                                                                                                                 cccGTCCGAGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATAAGCGAATTc                  >  1:754045/1‑149 (MQ=255)
                                                                                                                                    gTCCGGGTGCATGTTTCCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCtta               <  2:845207/149‑1 (MQ=255)
                                                                                                                                    gTCCGGGTGCATGTTTCCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCtta               <  2:849408/149‑1 (MQ=255)
                                                                                                                                         ggTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTattatt          >  2:538233/1‑149 (MQ=255)
                                                                                                                                         ggTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTattatt          <  2:425082/149‑1 (MQ=255)
                                                                                                                                          gTGCATGTTTTCACTTGGCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTg         <  1:633964/149‑1 (MQ=255)
                                                                                                                                           tGCATGTTTTCACTTGTCGGATATTAAAGCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGt        <  2:577769/149‑1 (MQ=255)
                                                                                                                                             cATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGtaa      <  1:866861/149‑1 (MQ=255)
                                                                                                                                                gTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGtaataa   >  2:253475/1‑149 (MQ=255)
                                                                                                                                                gTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGATGTTGGTGATAGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTGTTTCATCCCTGAATAATTGCCATCAGCGCATTCTTATTATTGtaataa   <  2:49555/149‑1 (MQ=255)
                                                                                                                                                 ttttCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAac  >  2:495246/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGTTGGATCGTTCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCGAGTCACATTGACAACTATCAGCCACCTGAACAGAGTGCTTCGGTACAACACAAGTAAGCTCTGCACTTGTGGAGCGACATGCTGCCCGTCCGGGTGCATGTTTTCACTTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAAC  >  NC_000913/1704575‑1704868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: