Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 78,576 G→A 6.8% G319D (GGC→GAC)  setA → broad specificity sugar efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091378,5760GA 6.8% 129.2 / 3.1 60G319D (GGC→GAC) setAbroad specificity sugar efflux system
Reads supporting (aligned to +/- strand):  ref base G (20/34);  new base A (2/2);  total (23/37)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

TGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGC  >  NC_000913/78433‑78722
                                                                                                                                               |                                                                                                                                                  
tggctACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCAttg                                                                                                                                               <  1:876507/149‑1 (MQ=255)
          gTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTAt                                                                                                                                     <  1:458612/149‑1 (MQ=255)
          gTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGAAGTGGCGGCAGGAGTACTGTTGTACACGGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAAAGCTGTATTTATCGGCATTGTTGCGGGTAt                                                                                                                                     >  2:726369/1‑149 (MQ=255)
           tCAAACGTTATGGTAAGCGGCGAATGATGGTCAAAGCAGTGGCGGCAGGAGGACTGTTTTAAACCGGATTGATTTTCTTTAATAGCCGTATGGCGATGATGAAGATGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCAGGTAtt                                                                                                                                    >  1:896595/1‑149 (MQ=255)
            cAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTg                                                                                                                                   <  2:233146/149‑1 (MQ=255)
              aaCGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCTACATTGTTGCGGGTATTggg                                                                                                                                 >  1:923029/1‑149 (MQ=255)
              aaCGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGACATTGTTGCGTGTATTggg                                                                                                                                 >  1:92833/1‑149 (MQ=255)
                  ttATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATATCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGc                                                                                                                             >  1:835296/1‑149 (MQ=255)
                   tATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATACCCGTATGGCGTTGATGACGCTGCAAAATTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCt                                                                                                                            <  1:412104/149‑1 (MQ=255)
                     tGGTAAGCGGCGCATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGAGTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTAt                                                                                                                          <  2:597493/149‑1 (MQ=255)
                           gcggcgAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTc                                                                                                                    >  1:917636/1‑149 (MQ=255)
                           cccgctaaTGATGGGCCTAGCCGTGGCGGCACGAGTACTGTATTACACCGGATTGATTTTCATTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTc                                                                                                                    <  2:748637/143‑1 (MQ=255)
                             ggcgAATGATGGGCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGAGTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAg                                                                                                                  <  1:87617/149‑1 (MQ=255)
                              gcgAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTAATAGCCGTATGGCGTTGATGACGCTTCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATCGATTCAgg                                                                                                                 >  1:657673/1‑149 (MQ=255)
                                gAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATTACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGAt                                                                                                               >  2:114689/1‑149 (MQ=255)
                                gAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTTACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGAt                                                                                                               >  1:24307/1‑149 (MQ=255)
                                gAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGAt                                                                                                               >  2:110907/1‑149 (MQ=255)
                                       ggTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGTCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTTTTGGGATGCTATGGTTTCAGGATTTTATGc                                                                                                        >  1:203885/1‑149 (MQ=255)
                                         tcctAGCAGTGGCGGCAGGAGTACTGTTTTACAACGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCTGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCt                                                                                                      <  2:738920/146‑1 (MQ=255)
                                          cATCGCAGTGGCGGCAGGAGTACTGTTTTACACCGGAATGATTTTCTTTAATCGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTg                                                                                                     <  1:172633/149‑1 (MQ=255)
                                          cATAGCAGGGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTAGGGTTTCAGGATTTAATGCCTg                                                                                                     <  1:176424/149‑1 (MQ=255)
                                             aGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGaa                                                                                                  <  2:448549/149‑1 (MQ=255)
                                                      ggcAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCggggg                                                                                         >  2:732031/1‑149 (MQ=255)
                                                      ggcAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGGTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGATGAGCtgggg                                                                                         >  1:719386/1‑149 (MQ=255)
                                                      ggcAGGAGTACTGATTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCggggg                                                                                         <  2:220410/149‑1 (MQ=255)
                                                        cAGGAGTACTGTTTTACACCGTATTGATTTTCTTTAATAGCCGTATGGAGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCa                                                                                       <  1:634847/149‑1 (MQ=255)
                                                             gtccTGCTTTACACCGGATTGAATTTCTTTAATAGCCCTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTAATAATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGAGTTAATGCCTGGAAGAGCGGGGGCAGCTac                                                                                  <  1:935635/146‑1 (MQ=255)
                                                             gTACGGTGTTCCCCCGGCTTGCTTTGCTTTCATAGCCGGAAGGCGTTGATGACGCTGCAACCTTTTACCGCTGTATCTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCGGGAAGAGCGGGGGCAGCTac                                                                                  <  2:492699/149‑1 (MQ=255)
                                                                      tACACCTGATTGATTTTCTTTAATCGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTGTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTAtt                                                                         <  2:738265/149‑1 (MQ=255)
                                                                       acacCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTAttt                                                                        >  2:66311/1‑149 (MQ=255)
                                                                         acCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCGGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTAc                                                                      <  1:965670/149‑1 (MQ=255)
                                                                           cGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTa                                                                    <  1:197315/149‑1 (MQ=255)
                                                                            ggATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTTTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTaa                                                                   <  2:463922/149‑1 (MQ=255)
                                                                            ggATTGATTTTCTTTAAACCCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTaa                                                                   <  2:992810/149‑1 (MQ=255)
                                                                                  attTTCTTTAATAGCCGTATGGCGTTGATGACGCGGCAACTTTTTAACGCTGTATTTCTCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTAt                                                             <  2:250857/149‑1 (MQ=255)
                                                                                   ttttCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTAtt                                                            <  1:834327/149‑1 (MQ=255)
                                                                                       cTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTa                                                        <  2:717254/149‑1 (MQ=255)
                                                                                        tttACTAGCCGTATGGCGTTGAGGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTTTGGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGTGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                       <  1:863212/149‑1 (MQ=255)
                                                                                         ttAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAcc                                                      >  1:515621/1‑149 (MQ=255)
                                                                                         ttAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAcc                                                      <  2:917636/149‑1 (MQ=255)
                                                                                           aaTAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGGATTGTTGCGGGTTTTGGGATGCTATGGTTTCATGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCgg                                                    <  2:519608/149‑1 (MQ=255)
                                                                                            aTAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCggg                                                   >  1:832225/1‑149 (MQ=255)
                                                                                            aTAGACGTATGGCGTTGATGACGCTGCAACAATATAACGCTGTATTTATCGTCATAGTTGCGGTTATTGTGAAGCTATGGTTTCAGGATTAAAAGCATGGAAGAGCGGTGGAAGCTACCACCTTAATTACTAACAGTATTTCTACCgcg                                                   >  2:354895/1‑147 (MQ=255)
                                                                                                     tGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTctg                                          >  1:130275/1‑149 (MQ=255)
                                                                                                            gctgccGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGGTGCGGGTATTGGGATGCTATGGTTTCAGTATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTATCAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                   <  2:210434/144‑1 (MQ=255)
                                                                                                            gatgaCGCTGCCACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTTTGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                   >  1:724702/1‑149 (MQ=255)
                                                                                                            gatgaCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCATGGAAGAGCTGGGTCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                   >  1:728368/1‑149 (MQ=255)
                                                                                                              tgaCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTTCCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGtt                                 <  1:114689/149‑1 (MQ=255)
                                                                                                              tgaCGCTGCAACTTTTTAACGCTGTAATTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTTCCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGtt                                 <  1:110907/149‑1 (MQ=255)
                                                                                                               gaCGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTa                                >  1:25837/1‑149 (MQ=255)
                                                                                                                 cgcgGCAACTTGTTAACCCCTCATTTATCGTCATTGGTGCGGGTATTGGGATGCTATGGGTGCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAATAGTATTACTACCGGGGTAATTCTGGCTGGCGTTAtt                              <  2:559638/149‑1 (MQ=255)
                                                                                                                   cTGCAACTTTTTCACGCTGTATTTATCGGCATTGTGGCGGGTATTGGGATGCTATGGTTTCAGGATTTTACGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCa                            <  1:127045/149‑1 (MQ=255)
                                                                                                                     gCAACTTTTTAACGCTGTATTTATCGGCATTGTTCCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGTGGCAGCTACCACCTTATTTACTAACAGTATTTCAACCGGGGTAATTCTGGCTGGCGTTATTCAgg                          <  1:958648/149‑1 (MQ=255)
                                                                                                                        aCTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAg                       >  2:239228/1‑149 (MQ=255)
                                                                                                                                        tttATCGGCATTGTTGCGGGTATTGGGATGCTATTGTTTCAGGATTTAATGCATGGTAGATCGGGGGCATGTACCACCTTATTTAATAACAGTATTTCTACCGGGGTAATTCTTGCTGTCGTTATTGATGTAGCAATTGCACAAAGTTg       <  2:657673/149‑1 (MQ=255)
                                                                                                                                         ttATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTgg      <  2:130275/149‑1 (MQ=255)
                                                                                                                                         ttATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTgg      <  2:755927/149‑1 (MQ=255)
                                                                                                                                          tATCGACATTTATGCGGGTATTTGGATGCTATCGTTTCAGGATTTACTGCCTGGAAGAGCGGGGGAAGCTTCCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTggg     <  2:923029/149‑1 (MQ=255)
                                                                                                                                          tATCGACATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTggg     <  2:92833/149‑1 (MQ=255)
                                                                                                                                             cGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTTTTTACTAACAGTATTTCTAACGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGc  >  1:802750/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGC  >  NC_000913/78433‑78722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑