Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,768 T→C 6.4% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7680TC 6.4% 172.1 / 3.0 64intergenic (+34/+14)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (26/33);  new base C (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTG  >  NC_000913/1864625‑1864915
                                                                                                                                               |                                                                                                                                                   
gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                                <  1:637679/149‑1 (MQ=255)
gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGc                                                                                                                                                <  2:509867/149‑1 (MQ=255)
 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                               >  1:53633/1‑149 (MQ=255)
 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                               <  1:455273/149‑1 (MQ=255)
   aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                             >  1:161823/1‑149 (MQ=255)
     aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcacaca                                                                                                                                           <  1:477356/149‑1 (MQ=255)
            ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcac                                                                                                                                    >  2:228902/1‑149 (MQ=255)
               tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                                 >  1:672284/1‑149 (MQ=255)
                 tgtgTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCat                                                                                                                               >  1:84637/1‑149 (MQ=255)
                  gtgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATCCATCCGGCACACAACATCACACCatt                                                                                                                              >  1:226564/1‑148 (MQ=255)
                                tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAATCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                                <  2:565789/149‑1 (MQ=255)
                                  cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                              <  1:305922/149‑1 (MQ=255)
                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCt                                                                                                            >  2:247056/1‑149 (MQ=255)
                                     ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATCCGGCAAACCACATCACCCCATATCCAGCGCAGTTTTTCAtt                                                                                                           >  1:913730/1‑149 (MQ=255)
                                      tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCttt                                                                                                          >  2:147803/1‑149 (MQ=255)
                                       cAGAAACGCAAAAAGTGACCAACTAGAAACCTGCACATCTGGCCCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                         <  2:807193/149‑1 (MQ=255)
                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                         <  1:388722/149‑1 (MQ=255)
                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTCCtttt                                                                                                         >  2:130002/1‑149 (MQ=255)
                                          aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                      <  2:405393/149‑1 (MQ=255)
                                              gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTgggggcg                                                                                                  >  1:714819/1‑144 (MQ=255)
                                               cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCAGTTTGGTTCCgg                                                                                                 >  2:113306/1‑149 (MQ=255)
                                                  aaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCCTCCGGCACCCAACCTCCCACCCTATCCAGCGCAGTTTTTCCTTTTGGTGCCGGata                                                                                              >  1:412512/1‑149 (MQ=255)
                                                     gtcaCCAATCAGAAACCTGATCTTCTTGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTCCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                           <  1:894471/146‑1 (MQ=255)
                                                     gTGCCCAACGAGAAACCTGCACCACTGCCGCACTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATCTTGTTTGCCGGGGGAAATGCATCCGGCACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                           <  2:489360/149‑1 (MQ=255)
                                                      tgcccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                          <  2:73015/146‑1 (MQ=255)
                                                        aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                        <  2:991725/149‑1 (MQ=255)
                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCAACCGGCACACAACATCACACAATATCCAGCTGAGTTTTTCCTTTTGGTGGCGGATATGCCGTATc                                                                                     >  1:475324/1‑149 (MQ=255)
                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACCTCACACCATATCCCGCGCAGTTTTTCCTGTTGGTGCCGGATATGCCTTATc                                                                                     >  2:807249/1‑149 (MQ=255)
                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                     >  1:318885/1‑149 (MQ=255)
                                                              gagaAACCTGGACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                                  <  1:713543/149‑1 (MQ=255)
                                                              gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTACGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                                  <  2:839565/149‑1 (MQ=255)
                                                                   aCCTGCACATCTGGCTCATTCCATTCTCGTGGCGAAACGTAACTTTAGGTTAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCTTATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAg                                                                             <  2:760375/149‑1 (MQ=255)
                                                                   aCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGATGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACAATATCAAGCTCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATac                                                                             >  1:601026/1‑148 (MQ=255)
                                                                              tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                  <  1:113306/149‑1 (MQ=255)
                                                                              tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                  >  1:531484/1‑149 (MQ=255)
                                                                                         aatCGCGTTGCGAAACGTTAATTTACGTTACTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCACCGCAGTTTTTCCTATTGGTGCCGGATATGCCTTATCCAGCATAGCTAAATCCGCTGAAGAAAGTTc                                                       <  2:328774/147‑1 (MQ=255)
                                                                                         aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                                       >  1:710631/1‑149 (MQ=255)
                                                                                            ggcgTTGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAc                                                    <  1:759072/148‑1 (MQ=255)
                                                                                                  gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                              <  2:161823/149‑1 (MQ=255)
                                                                                                   cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGc                                             >  2:380094/1‑149 (MQ=255)
                                                                                                     aaaCGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                           <  1:846797/149‑1 (MQ=255)
                                                                                                         gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                       >  1:313415/1‑149 (MQ=255)
                                                                                                            aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCa                                    <  2:672284/149‑1 (MQ=255)
                                                                                                                 aCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttg                               <  2:53633/149‑1 (MQ=255)
                                                                                                                  cGTTAATGTTGTGTGCCGGGTTCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgt                              <  2:757285/149‑1 (MQ=255)
                                                                                                                  cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgt                              <  2:80259/149‑1 (MQ=255)
                                                                                                                   gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                             <  2:138203/149‑1 (MQ=255)
                                                                                                                   gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                             <  1:629679/149‑1 (MQ=255)
                                                                                                                    ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTACGCTGAAGAAAGTTCGAACTCAAGCACAGCCGCATTttgttg                            >  2:622747/1‑149 (MQ=255)
                                                                                                                    ttAATGTTGTGTGACGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGTTGCCGTATATGCCTTATCCAGCATAGCTAATTCCGCTTAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                            <  1:416905/149‑1 (MQ=255)
                                                                                                                     tAATGTTGTGTGCCGGGTGCAATTCATCCGGCCCACAACATCACAACATATACAGCGCAGTTTTTACGTTTGGTGCCGGATATGCCTTATCCAGCATATCAAATTCCGCTGAAGAAAGTTCGACATCCAGCACAGCCGCATTTTGATgg                           >  1:254701/1‑149 (MQ=255)
                                                                                                                         gttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                       <  2:19194/149‑1 (MQ=255)
                                                                                                                             tgtgCCGGGTGCAATGCATCCGGCACAAAACATCACACCTTATCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCTCATTTTGTTGGACATGGGc                   <  2:381342/149‑1 (MQ=255)
                                                                                                                               tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGACGGATATGCCTTATCCAGCATAGCTAATTCCTCTGAAGAAAGTTCGACCTCAAGCACAGCAGCATTTTGTTGTACATGGGCaa                 >  2:350910/1‑149 (MQ=255)
                                                                                                                               tgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGCCATGGGCaa                 >  2:969567/1‑149 (MQ=255)
                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACCCCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc               >  1:324640/1‑149 (MQ=255)
                                                                                                                                    ggTGCAATGCATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAATGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg            <  1:780622/149‑1 (MQ=255)
                                                                                                                                    ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg            <  1:981683/149‑1 (MQ=255)
                                                                                                                                    gatGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg            >  2:231215/3‑149 (MQ=255)
                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg           <  2:710631/149‑1 (MQ=255)
                                                                                                                                           tGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtt     <  2:382937/149‑1 (MQ=255)
                                                                                                                                            gCATCCGGCACACACCAGCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCTTTTTGGTGGACATGGGCAATCGTGGCCGCgtt    >  2:853039/1‑149 (MQ=255)
                                                                                                                                             cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtttt   <  2:494589/149‑1 (MQ=255)
                                                                                                                                              aTCCGGCACACAACATCACCCCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAGCGTGGCCGCTTTTg  >  2:174803/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTG  >  NC_000913/1864625‑1864915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑