Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,904,041 A→T 5.8% A49A (GCA→GCT)  manZ → mannose‑specific enzyme IID component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,904,0410AT 5.8% 187.0 / 2.4 69A49A (GCA→GCT) manZmannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base T (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

AATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGA  >  NC_000913/1903894‑1904189
                                                                                                                                                   |                                                                                                                                                    
aaTGGTTGATTCAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCaa                                                                                                                                                     <  1:432826/149‑1 (MQ=255)
      tGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATtcgtc                                                                                                                                               <  2:977027/149‑1 (MQ=255)
      tGAGACAACTCAAACTCCCCCCGACAAAAACCTCACTCACAGTGATATTCGAGGCGTCTTCCTGCGCTCTCCCCTCTTCCAGGGCGCATGGACCTTCGACCGTATGCAGGCACTGGGGGTCTGCATCTCTATGGTACCGGCAATtcggc                                                                                                                                               <  2:917561/149‑3 (MQ=255)
        ttACAACTCAAACTACCACCGATAAAAAACTAACTCAAAGTGATATTCGTGGCGGCTTCCTGCGTTCTAACCTCTTCCAGGGGTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGc                                                                                                                                             <  1:970891/148‑1 (MQ=255)
          aCAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCct                                                                                                                                           <  2:49899/149‑1 (MQ=255)
          aCAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGGTTCTGCTGATCTATGGTACCGGCAATTCGTCGCct                                                                                                                                           >  2:688124/1‑149 (MQ=255)
            aaCTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCgtct                                                                                                                                         >  2:490680/1‑149 (MQ=255)
            aaCTCAAACTACCACAGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCctct                                                                                                                                         <  2:979249/149‑1 (MQ=255)
              cTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTAc                                                                                                                                       >  2:406626/1‑149 (MQ=255)
                cAAACTCCCAACGAGAAAAAACTCACTCAAAGTGATATTCGTGTATTCTTCCTGCGGTCTAACCTCTTCCAGGGTTCATTGCACTTCGAACGTATGCAGGCAATGGGATTCTGCTTATCTATTGTACCGGCAATTCGTCGCCTCTAcac                                                                                                                                     >  2:899712/1‑147 (MQ=255)
                 aaaCTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTGATGGAACTTCGAACGTATGCAGGCACGGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTAcccg                                                                                                                                    >  1:910228/1‑148 (MQ=255)
                   aCTACCACCGAGAAAAAACTCACTCAACGTGATATTCGTGGCGGCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTga                                                                                                                                  <  2:771767/149‑1 (MQ=255)
                       ccaccGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGaac                                                                                                                              >  2:933664/1‑149 (MQ=255)
                        caccGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGaaca                                                                                                                             >  1:631999/1‑149 (MQ=255)
                         accGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGaacaa                                                                                                                            <  1:730357/149‑1 (MQ=255)
                          ccGAGAAAAAAGTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGaacaac                                                                                                                           <  2:903442/149‑1 (MQ=255)
                               aaaaaaCTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGc                                                                                                                      >  2:171476/1‑149 (MQ=255)
                                 aaaaCTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTc                                                                                                                    <  1:388687/149‑1 (MQ=255)
                                  aaaCTCACTCAAAGTTATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGATAACAACGAAGCTCg                                                                                                                   <  1:615228/149‑1 (MQ=255)
                                   aaCTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGt                                                                                                                  <  2:984638/149‑1 (MQ=255)
                                    actcactcAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTa                                                                                                                 >  1:682682/1‑149 (MQ=255)
                                       cactcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAAc                                                                                                              >  2:141352/1‑149 (MQ=255)
                                                 gATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCg                                                                                                    >  2:722043/1‑149 (MQ=255)
                                                  atatTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGc                                                                                                   >  2:527993/1‑149 (MQ=255)
                                                    atTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTAGCCg                                                                                                 >  1:550449/1‑149 (MQ=255)
                                                           gCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGACCGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTg                                                                                          <  2:123759/149‑1 (MQ=255)
                                                           gCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTg                                                                                          >  2:739973/1‑149 (MQ=255)
                                                             gTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGa                                                                                        <  2:378279/149‑1 (MQ=255)
                                                             gTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGa                                                                                        >  2:1023556/1‑149 (MQ=255)
                                                                ttCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGtt                                                                                     <  2:311047/149‑1 (MQ=255)
                                                                   cTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCtt                                                                                  <  1:963291/149‑1 (MQ=255)
                                                                   cTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCtt                                                                                  <  1:141352/149‑1 (MQ=255)
                                                                   cTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGATTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCtt                                                                                  >  2:614196/1‑149 (MQ=255)
                                                                      cGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTCGTTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTAACCTGAGAACAACGAAGATCGTAAACAAGCTATTCGACGTCAACGTGAGTTCTTTaa                                                                               >  2:611517/1‑149 (MQ=255)
                                                                           tAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACAccc                                                                          <  2:514347/149‑1 (MQ=255)
                                                                              ccTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGc                                                                       >  2:446795/1‑149 (MQ=255)
                                                                               ctctTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTAACCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCAACGGGAGTGATTTAACACCAAGcc                                                                      >  1:993603/1‑149 (MQ=255)
                                                                                    ccAGGGTTCATGGAACTTCGCACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTACACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGGTCg                                                                 >  2:415937/1‑149 (MQ=255)
                                                                                    ccAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCTATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTg                                                                                          >  2:30437/1‑124 (MQ=255)
                                                                                    ccAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCTATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTg                                                                                          <  1:30437/124‑1 (MQ=255)
                                                                                    ccAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCTATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTg                                                                                          <  1:26444/124‑1 (MQ=255)
                                                                                    ccAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCTATTCGTCGCCTCTACCATGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTg                                                                                          >  2:26444/1‑124 (MQ=255)
                                                                                      aGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTg                                                               >  1:778109/1‑149 (MQ=255)
                                                                                      aGGGTTCATGGAACTTCGAACGTATGCAGGCACGGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAACCAAGCTAATCGCCGTCACCGGGAGTTCTTTAACACCCAGCCGTTCggg                                                               >  2:910516/1‑147 (MQ=255)
                                                                                                    ttCGAACGTATGCAGGCACTGGGTTTCTGCGTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTCACACCCAGCCGTTCGTGGCTGCGCCGAGtct                                                 >  1:481571/1‑149 (MQ=255)
                                                                                                        aaagTATGCAGGCACTGGGTTTCTGCTGCTCTTTGGTACCGGCAATTCGTCGCCTCTACACTGAGAACAACGTAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCgtc                                             <  2:910228/146‑3 (MQ=255)
                                                                                                        aaCGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCTCCGTCACCTGGAGTTATTTAACACACAGCAGTTCGTGGCTGCTCAGCTTCTCGGc                                             >  1:246310/1‑149 (MQ=255)
                                                                                                             aTGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAAc                                        >  1:968689/1‑149 (MQ=255)
                                                                                                                  ggCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTgg                                   >  2:32167/1‑149 (MQ=255)
                                                                                                                   gCACTGGGATTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACACAGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTGATTTGACACCCAGCCCTGCCTGGCTGCGCCGCTTCTCGCCGTCACCCTgtc                                  >  1:644597/1‑147 (MQ=255)
                                                                                                                          gTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGGCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGgaa                           <  2:34500/149‑1 (MQ=255)
                                                                                                                             tCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTCCCCTGAGAACAACGAAGCTCGTAAACTAGCTATTCGCCGTCTCCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGgaagaa                        <  2:682682/149‑1 (MQ=255)
                                                                                                                             tCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGgaagaa                        <  1:171476/149‑1 (MQ=255)
                                                                                                                               tGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACTCCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACa                      <  1:189143/149‑1 (MQ=255)
                                                                                                                                 cTTCTCTTTGGTACCGGCAATTCGTCGCCTCTTCCCTGAGAACAACGAAGCTCGTAAACAAGGTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGc                    <  2:763202/149‑1 (MQ=255)
                                                                                                                                 cTTCTCTATGGTACCGTCAATTCGTCTCCTCTACCCTGAGAACATCGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCTGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGc                    <  1:524647/149‑1 (MQ=255)
                                                                                                                                 cTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGc                    <  2:631999/149‑1 (MQ=255)
                                                                                                                                    ctctATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACACGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTg                 <  2:922715/149‑1 (MQ=255)
                                                                                                                                       tATGGTACCGGCAATTCGTCGGCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTTTTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTGTCGGGGTAACCCTGGCGCTGGAGGCACAGCGTGCTa              >  2:362567/1‑149 (MQ=255)
                                                                                                                                       tATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGCACAGCGTGCTa              >  1:742807/1‑149 (MQ=255)
                                                                                                                                       tATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTa              >  1:431011/1‑149 (MQ=255)
                                                                                                                                         tGGTACCGGCACTTCGTCCCCGCTACCCAGACAACACCGAAGCGCCTACCCAACCTCTTCGCCGGCACCTGGACGGCTTTAACACCCAGCCGTTCGTGGCCGCGCCGATGCTCGGCGTAACCCGGGCGCTGGAAGAACAGCGTGCGAAt            <  2:741530/149‑1 (MQ=255)
                                                                                                                                         tGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTTGTATACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCTGCGAGGCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTTAt            >  2:888396/1‑149 (MQ=255)
                                                                                                                                          ggTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATg           >  1:874039/1‑149 (MQ=255)
                                                                                                                                           gTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTGCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATgg          <  2:242147/149‑1 (MQ=255)
                                                                                                                                            tACCGGCAATTCGTCGCCTCTACGCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGgc         <  2:550449/149‑1 (MQ=255)
                                                                                                                                            tACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCCGGCGCTGGAAGAACAGCGTGCTAATGgc         <  1:233347/149‑1 (MQ=255)
                                                                                                                                                ggCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCag     <  2:778109/149‑1 (MQ=255)
                                                                                                                                                 gCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTAAACACCCAGCCCTTCGTGGCGGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAACAACAGCGTGCTAATGGCGCaga    >  2:475630/1‑149 (MQ=255)
                                                                                                                                                  cAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCagag   >  2:52692/1‑149 (MQ=255)
                                                                                                                                                   aaTTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAgcga  >  2:553562/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATGGTTGATACAACTCAAACTACCACCGAGAAAAAACTCACTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGA  >  NC_000913/1903894‑1904189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑