Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,059,946 T→A 12.3% intergenic (+20/+18) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,059,9460TA12.3% 98.5 / 4.3 46intergenic (+20/+18)asnU/cbltRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (23/17);  new base A (3/3);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTT  >  NC_000913/2059800‑2060092
                                                                                                                                                  |                                                                                                                                                  
aTCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCtttt                                                                                                                                                  <  1:659176/149‑1 (MQ=255)
 tCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTta                                                                                                                                                 <  2:550261/149‑1 (MQ=255)
   tgtttttACTAAATATTTTTCGCCGTTAAGATGTGCCTCAACAACTATTCCTCTGTAGTGCAGTCGGTAGAACGGCGGACTGTTAATCCGTCTGTCACTGGCTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTtata                                                                                                                                               <  2:946734/146‑1 (MQ=21)
      tttGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCg                                                                                                                                            <  2:407498/149‑1 (MQ=255)
        tGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCggg                                                                                                                                          <  1:88483/149‑1 (MQ=255)
           cAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTGCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTATAAAAGCGGGCtt                                                                                                                                       <  2:948744/149‑1 (MQ=21)
            aaaaGATTTTTCGCCCTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGACACTGCTTCGAGTCCAGTAAGAGGAGCCAAATTCCTGAAAAGCCCCCTTTTATAGCAGCAttt                                                                                                                                      >  1:874857/1‑149 (MQ=21)
               aGATTTTTCGCCGTGAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttca                                                                                                                                   <  2:480494/149‑3 (MQ=18)
                gATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCCGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAACGCCCGCTTTTATAGCGGGATTTTTGc                                                                                                                                  <  1:860888/149‑1 (MQ=255)
                gATATTTCGCAGTTACGATGTGCCTCAACAACGATCCCTCAGTCGTGCAGTCAGTAGAACGGCGGACTTTTAATCCGTAGGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGc                                                                                                                                  <  1:859428/149‑1 (MQ=21)
                         ccGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGCCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGCGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                         >  2:576271/1‑149 (MQ=255)
                            ttAAGATGTGCCTCAACAACGCTTCCTCTGTAGTTCAGTCGGTAGAACGGCGGGCTGTTAATCCGGATGGCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATGTTTGGTATATCTGATaa                                                                                                                      >  2:608192/1‑149 (MQ=21)
                            ttAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATaa                                                                                                                      >  1:147811/1‑149 (MQ=255)
                                 aTGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCCTATGTCACTGGTTCGAGTCCAGACAGAGGAGCCAAAATCCAGAAAAGCCCGCTTTTAAAGCGGGATTTTTGCTATATCTGATAATCAAt                                                                                                                 >  1:74453/1‑149 (MQ=21)
                                     gCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTcc                                                                                                             >  2:508582/1‑149 (MQ=255)
                                      ccTCAACAACGATTCCTCTGTAGTTCATTCGGTAGAACGGCGGACTGTTACTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCct                                                                                                            <  2:909332/149‑1 (MQ=255)
                                          aacaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTc                                                                                                        >  2:803874/1‑149 (MQ=255)
                                          aacaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTc                                                                                                        >  1:551532/1‑149 (MQ=255)
                                           acaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCa                                                                                                       <  1:235371/149‑1 (MQ=255)
                                                       ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTATTATAGCGGGATTTTTGCTAAATCTGATAATCAATTTCCTCTTCACTGCTTTCCAta                                                                                           >  2:868942/1‑148 (MQ=255)
                                                         gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                                                                         >  1:730587/1‑149 (MQ=255)
                                                            gTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTg                                                                                      <  2:294007/149‑1 (MQ=255)
                                                                  tCGGTAGAACGGCGGACAGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGCATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTACCATCACCTGCCGCta                                                                                >  1:190483/1‑148 (MQ=255)
                                                                      tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGata                                                                            <  2:631307/149‑1 (MQ=255)
                                                                       aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCCCCTGCCGCTTGatat                                                                           >  2:234531/1‑149 (MQ=255)
                                                                           cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTGCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTc                                                                       <  1:1006429/149‑1 (MQ=255)
                                                                                gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCCTCCCCTGCCGCTTGATATCCTCAACTg                                                                  >  2:556178/1‑149 (MQ=255)
                                                                                   tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACa                                                               <  1:111481/149‑1 (MQ=255)
                                                                                     ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTGTTCACTGCTATCGATCACCTGCCGCTTGATATCCTCAACTGAGAGt                                                             >  1:1002266/1‑149 (MQ=255)
                                                                                       aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCGTTTATAGCGGGATTTTAGCTATATCTTATAATAAATTTCCTCTTCACTGCTTTGCATCACATGCCGCTTGTTATCCTCAAATGACAGttg                                                           >  1:961825/1‑147 (MQ=37)
                                                                                            gTATGTCTCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAt                                                      <  2:998076/149‑1 (MQ=255)
                                                                                             tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                     >  2:717114/1‑149 (MQ=255)
                                                                                               tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTAc                                                   <  1:250550/149‑1 (MQ=255)
                                                                                                gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                  >  2:247206/1‑149 (MQ=255)
                                                                                                  cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaaa                                                <  1:345519/149‑1 (MQ=255)
                                                                                                         tCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCGATCACCTGCCGCGTGATATCCTCTACTGACAGGCCTGCATTACAATGTTCAAg                                         >  1:431795/1‑149 (MQ=255)
                                                                                                           gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTGCAgta                                       >  1:96722/1‑147 (MQ=255)
                                                                                                           gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGaa                                       >  1:508766/1‑149 (MQ=255)
                                                                                                              tCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCCTCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgc                                    >  1:206349/1‑149 (MQ=255)
                                                                                                                cAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                  <  2:410748/149‑1 (MQ=255)
                                                                                                                            ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTa                      >  1:146299/1‑149 (MQ=255)
                                                                                                                                     tGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGt             >  2:337816/1‑149 (MQ=255)
                                                                                                                                     caaaaaTCCCGCTATAAAAGCGGGCTTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGAAATCCTCAACTGACAGTCCTGCATTTCAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGt             >  2:107574/3‑149 (MQ=255)
                                                                                                                                      gAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGtt            >  2:756514/1‑149 (MQ=255)
                                                                                                                                        aaaTCCCGCTATAAAAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGt          >  2:219879/1‑149 (MQ=255)
                                                                                                                                          aGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCCCAACTGACAGGCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTcc        <  1:576271/149‑1 (MQ=255)
                                                                                                                                                cTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCtt  <  2:716632/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTT  >  NC_000913/2059800‑2060092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑