Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,059,948 T→A 11.4% intergenic (+22/+16) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,059,9480TA11.4% 93.9 / 4.8 44intergenic (+22/+16)asnU/cbltRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (24/15);  new base A (2/3);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

ATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAG  >  NC_000913/2059800‑2060095
                                                                                                                                                    |                                                                                                                                                   
aTCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCtttt                                                                                                                                                     <  1:659176/149‑1 (MQ=255)
 tCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTta                                                                                                                                                    <  2:550261/149‑1 (MQ=255)
   tgtttttACTAAATATTTTTCGCCGTTAAGATGTGCCTCAACAACTATTCCTCTGTAGTGCAGTCGGTAGAACGGCGGACTGTTAATCCGTCTGTCACTGGCTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTtata                                                                                                                                                  <  2:946734/146‑1 (MQ=21)
      tttGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCg                                                                                                                                               <  2:407498/149‑1 (MQ=255)
        tGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCggg                                                                                                                                             <  1:88483/149‑1 (MQ=255)
           cAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTGCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTATAAAAGCGGGCtt                                                                                                                                          <  2:948744/149‑1 (MQ=21)
            aaaaGATTTTTCGCCCTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGACACTGCTTCGAGTCCAGTAAGAGGAGCCAAATTCCTGAAAAGCCCCCTTTTATAGCAGCAttt                                                                                                                                         >  1:874857/1‑149 (MQ=21)
               aGATTTTTCGCCGTGAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttca                                                                                                                                      <  2:480494/149‑3 (MQ=18)
                gATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCCGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAACGCCCGCTTTTATAGCGGGATTTTTGc                                                                                                                                     <  1:860888/149‑1 (MQ=255)
                gATATTTCGCAGTTACGATGTGCCTCAACAACGATCCCTCAGTCGTGCAGTCAGTAGAACGGCGGACTTTTAATCCGTAGGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGc                                                                                                                                     <  1:859428/149‑1 (MQ=21)
                         ccGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGCCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGCGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                            >  2:576271/1‑149 (MQ=255)
                            ttAAGATGTGCCTCAACAACGCTTCCTCTGTAGTTCAGTCGGTAGAACGGCGGGCTGTTAATCCGGATGGCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATGTTTGGTATATCTGATaa                                                                                                                         >  2:608192/1‑149 (MQ=21)
                            ttAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATaa                                                                                                                         >  1:147811/1‑149 (MQ=255)
                                 aTGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCCTATGTCACTGGTTCGAGTCCAGACAGAGGAGCCAAAATCCAGAAAAGCCCGCTTTTAAAGCGGGATTTTTGCTATATCTGATAATCAAt                                                                                                                    >  1:74453/1‑149 (MQ=21)
                                     gCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTcc                                                                                                                >  2:508582/1‑149 (MQ=255)
                                      ccTCAACAACGATTCCTCTGTAGTTCATTCGGTAGAACGGCGGACTGTTACTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCct                                                                                                               <  2:909332/149‑1 (MQ=255)
                                          aacaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTc                                                                                                           >  2:803874/1‑149 (MQ=255)
                                          aacaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTc                                                                                                           >  1:551532/1‑149 (MQ=255)
                                           acaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCa                                                                                                          <  1:235371/149‑1 (MQ=255)
                                                       ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTATTATAGCGGGATTTTTGCTAAATCTGATAATCAATTTCCTCTTCACTGCTTTCCAta                                                                                              >  2:868942/1‑148 (MQ=255)
                                                         gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                                                                            >  1:730587/1‑149 (MQ=255)
                                                            gTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTg                                                                                         <  2:294007/149‑1 (MQ=255)
                                                                  tCGGTAGAACGGCGGACAGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGCATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTACCATCACCTGCCGCta                                                                                   >  1:190483/1‑148 (MQ=255)
                                                                      tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGata                                                                               <  2:631307/149‑1 (MQ=255)
                                                                       aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCCCCTGCCGCTTGatat                                                                              >  2:234531/1‑149 (MQ=255)
                                                                           cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTGCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTc                                                                          <  1:1006429/149‑1 (MQ=255)
                                                                                gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCCTCCCCTGCCGCTTGATATCCTCAACTg                                                                     >  2:556178/1‑149 (MQ=255)
                                                                                   tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACa                                                                  <  1:111481/149‑1 (MQ=255)
                                                                                     ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTGTTCACTGCTATCGATCACCTGCCGCTTGATATCCTCAACTGAGAGt                                                                >  1:1002266/1‑149 (MQ=255)
                                                                                       aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCGTTTATAGCGGGATTTTAGCTATATCTTATAATAAATTTCCTCTTCACTGCTTTGCATCACATGCCGCTTGTTATCCTCAAATGACAGttg                                                              >  1:961825/1‑147 (MQ=37)
                                                                                            gTATGTCTCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAt                                                         <  2:998076/149‑1 (MQ=255)
                                                                                             tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                        >  2:717114/1‑149 (MQ=255)
                                                                                               tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTAc                                                      <  1:250550/149‑1 (MQ=255)
                                                                                                gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                     >  2:247206/1‑149 (MQ=255)
                                                                                                  cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaaa                                                   <  1:345519/149‑1 (MQ=255)
                                                                                                         tCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCGATCACCTGCCGCGTGATATCCTCTACTGACAGGCCTGCATTACAATGTTCAAg                                            >  1:431795/1‑149 (MQ=255)
                                                                                                           gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTGCAgta                                          >  1:96722/1‑147 (MQ=255)
                                                                                                           gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGaa                                          >  1:508766/1‑149 (MQ=255)
                                                                                                              tCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCCTCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgc                                       >  1:206349/1‑149 (MQ=255)
                                                                                                                cAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                     <  2:410748/149‑1 (MQ=255)
                                                                                                                            ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTa                         >  1:146299/1‑149 (MQ=255)
                                                                                                                                     tGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGt                >  2:337816/1‑149 (MQ=255)
                                                                                                                                     caaaaaTCCCGCTATAAAAGCGGGCTTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGAAATCCTCAACTGACAGTCCTGCATTTCAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGt                >  2:107574/3‑149 (MQ=255)
                                                                                                                                      gAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGtt               >  2:756514/1‑149 (MQ=255)
                                                                                                                                        aaaTCCCGCTATAAAAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGt             >  2:219879/1‑149 (MQ=255)
                                                                                                                                          aGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCCCAACTGACAGGCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTcc           <  1:576271/149‑1 (MQ=255)
                                                                                                                                                cTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCtt     <  2:716632/149‑1 (MQ=255)
                                                                                                                                                   ttATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAg  <  2:730587/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCGGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAG  >  NC_000913/2059800‑2060095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑