Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,059,950 T→A 12.9% intergenic (+24/+14) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,059,9500TA12.9% 92.1 / 5.8 45intergenic (+24/+14)asnU/cbltRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (23/16);  new base A (3/3);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAG  >  NC_000913/2059803‑2060095
                                                                                                                                                   |                                                                                                                                                 
tgtttttACTAAATATTTTTCGCCGTTAAGATGTGCCTCAACAACTATTCCTCTGTAGTGCAGTCGGTAGAACGGCGGACTGTTAATCCGTCTGTCACTGGCTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTtata                                                                                                                                                  <  2:946734/146‑1 (MQ=21)
   tttGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCg                                                                                                                                               <  2:407498/149‑1 (MQ=255)
     tGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCggg                                                                                                                                             <  1:88483/149‑1 (MQ=255)
        cAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTGCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTATAAAAGCGGGCtt                                                                                                                                          <  2:948744/149‑1 (MQ=21)
         aaaaGATTTTTCGCCCTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGACACTGCTTCGAGTCCAGTAAGAGGAGCCAAATTCCTGAAAAGCCCCCTTTTATAGCAGCAttt                                                                                                                                         >  1:874857/1‑149 (MQ=21)
            aGATTTTTCGCCGTGAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttca                                                                                                                                      <  2:480494/149‑3 (MQ=18)
             gATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCCGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAACGCCCGCTTTTATAGCGGGATTTTTGc                                                                                                                                     <  1:860888/149‑1 (MQ=255)
             gATATTTCGCAGTTACGATGTGCCTCAACAACGATCCCTCAGTCGTGCAGTCAGTAGAACGGCGGACTTTTAATCCGTAGGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGc                                                                                                                                     <  1:859428/149‑1 (MQ=21)
                      ccGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGCCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGCGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                            >  2:576271/1‑149 (MQ=255)
                         ttAAGATGTGCCTCAACAACGCTTCCTCTGTAGTTCAGTCGGTAGAACGGCGGGCTGTTAATCCGGATGGCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATGTTTGGTATATCTGATaa                                                                                                                         >  2:608192/1‑149 (MQ=21)
                         ttAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATaa                                                                                                                         >  1:147811/1‑149 (MQ=255)
                              aTGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCCTATGTCACTGGTTCGAGTCCAGACAGAGGAGCCAAAATCCAGAAAAGCCCGCTTTTAAAGCGGGATTTTTGCTATATCTGATAATCAAt                                                                                                                    >  1:74453/1‑149 (MQ=21)
                                  gCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTcc                                                                                                                >  2:508582/1‑149 (MQ=255)
                                   ccTCAACAACGATTCCTCTGTAGTTCATTCGGTAGAACGGCGGACTGTTACTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCct                                                                                                               <  2:909332/149‑1 (MQ=255)
                                       aacaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTc                                                                                                           >  2:803874/1‑149 (MQ=255)
                                       aacaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTc                                                                                                           >  1:551532/1‑149 (MQ=255)
                                        acaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCa                                                                                                          <  1:235371/149‑1 (MQ=255)
                                                    ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTATTATAGCGGGATTTTTGCTAAATCTGATAATCAATTTCCTCTTCACTGCTTTCCAta                                                                                              >  2:868942/1‑148 (MQ=255)
                                                      gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                                                                            >  1:730587/1‑149 (MQ=255)
                                                         gTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTg                                                                                         <  2:294007/149‑1 (MQ=255)
                                                               tCGGTAGAACGGCGGACAGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGCATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTACCATCACCTGCCGCta                                                                                   >  1:190483/1‑148 (MQ=255)
                                                                   tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGata                                                                               <  2:631307/149‑1 (MQ=255)
                                                                    aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCCCCTGCCGCTTGatat                                                                              >  2:234531/1‑149 (MQ=255)
                                                                        cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTGCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTc                                                                          <  1:1006429/149‑1 (MQ=255)
                                                                             gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCCTCCCCTGCCGCTTGATATCCTCAACTg                                                                     >  2:556178/1‑149 (MQ=255)
                                                                                tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACa                                                                  <  1:111481/149‑1 (MQ=255)
                                                                                  ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTGTTCACTGCTATCGATCACCTGCCGCTTGATATCCTCAACTGAGAGt                                                                >  1:1002266/1‑149 (MQ=255)
                                                                                    aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCGTTTATAGCGGGATTTTAGCTATATCTTATAATAAATTTCCTCTTCACTGCTTTGCATCACATGCCGCTTGTTATCCTCAAATGACAGttg                                                              >  1:961825/1‑147 (MQ=37)
                                                                                         gTATGTCTCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAt                                                         <  2:998076/149‑1 (MQ=255)
                                                                                          tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                        >  2:717114/1‑149 (MQ=255)
                                                                                            tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTAc                                                      <  1:250550/149‑1 (MQ=255)
                                                                                             gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                     >  2:247206/1‑149 (MQ=255)
                                                                                               cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaaa                                                   <  1:345519/149‑1 (MQ=255)
                                                                                                      tCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCGATCACCTGCCGCGTGATATCCTCTACTGACAGGCCTGCATTACAATGTTCAAg                                            >  1:431795/1‑149 (MQ=255)
                                                                                                        gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTGCAgta                                          >  1:96722/1‑147 (MQ=255)
                                                                                                        gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGaa                                          >  1:508766/1‑149 (MQ=255)
                                                                                                           tCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCCTCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgc                                       >  1:206349/1‑149 (MQ=255)
                                                                                                             cAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                     <  2:410748/149‑1 (MQ=255)
                                                                                                                         ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTa                         >  1:146299/1‑149 (MQ=255)
                                                                                                                                  tGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGt                >  2:337816/1‑149 (MQ=255)
                                                                                                                                  caaaaaTCCCGCTATAAAAGCGGGCTTTTTGCTATATCTGATAATCAAATTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGAAATCCTCAACTGACAGTCCTGCATTTCAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGt                >  2:107574/3‑149 (MQ=255)
                                                                                                                                   gAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGtt               >  2:756514/1‑149 (MQ=255)
                                                                                                                                     aaaTCCCGCTATAAAAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGt             >  2:219879/1‑149 (MQ=255)
                                                                                                                                       aGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCCCAACTGACAGGCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTcc           <  1:576271/149‑1 (MQ=255)
                                                                                                                                             cTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCtt     <  2:716632/149‑1 (MQ=255)
                                                                                                                                                ttATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAg  <  2:730587/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGGTTTGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAG  >  NC_000913/2059803‑2060095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑