Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 125,817 A→G 11.0% K41K (AAA→AAG)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913125,8170AG11.0% 132.5 / 7.5 54K41K (AAA→AAG) aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base G (2/4);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

CGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGA  >  NC_000913/125669‑125963
                                                                                                                                                    |                                                                                                                                                  
cgTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGAACCGGACATCGGGGCTTATGAAGTTGAAATCACCGAGATCCTGTTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACaaa                                                                                                                                                    <  2:308416/149‑1 (MQ=255)
  tCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                                                                                                                  <  1:349440/149‑1 (MQ=255)
     ggCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCatc                                                                                                                                               >  1:1263/1‑149 (MQ=255)
      gCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCctct                                                                                                                                              >  2:653798/1‑149 (MQ=255)
       cgaaaGACGTAAACGACAAAAGGCTATCGAACTCAAAGTACCGGAAATCGGGGCTGATGAAGGTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa                                                                                                                                             <  2:284445/146‑1 (MQ=21)
        gTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTAt                                                                                                                                            <  2:758454/149‑1 (MQ=255)
        gTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCAACGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGACGCTGATCACCGTAGAAGGCGACAAAGCCTCTAt                                                                                                                                            >  1:580912/1‑149 (MQ=255)
         tAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATg                                                                                                                                           <  2:421337/149‑1 (MQ=255)
             agGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAg                                                                                                                                       >  1:505204/1‑149 (MQ=255)
             agGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAg                                                                                                                                       >  1:846711/1‑149 (MQ=255)
                  aaggAATAATGGCGATCGAAATCACAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGATCAGTCGCTGATCACCGTTGACGGCGACAAAGCCTCTATGGACGTTCCg                                                                                                                                  >  1:612898/4‑149 (MQ=255)
                  aaaGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCg                                                                                                                                  >  2:351266/1‑149 (MQ=255)
                      aataatGGCTATCGAAAGAAAAGGACCGGACATCGGGGCCGATGAATTTGAAATCACCGAGAACCTGGTCAAAGTGGGGGACAAAGTTGAAGCCGAACCGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGtctc                                                                                                                              <  1:666921/149‑1 (MQ=25)
                       ataatGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTcc                                                                                                                             >  2:936416/1‑149 (MQ=255)
                         tgtGGCTATCGACTTTAAAGTTCCGGATATCGGGCCTGACGAAGTTGAATTCACCGATATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCgt                                                                                                                           <  1:176122/147‑2 (MQ=11)
                                aTCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCggg                                                                                                                    >  2:127359/1‑149 (MQ=255)
                                   gAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACTGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTAt                                                                                                                 >  2:598445/1‑149 (MQ=255)
                                          aaGTACCGGACATCGGGGCTGATGACGTTGAAATCACCGAGATCCTGGGCAACGTGGGCGACAACGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTaaa                                                                                                          <  1:949381/149‑1 (MQ=255)
                                            gTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAaga                                                                                                        <  2:333711/149‑1 (MQ=255)
                                              aCCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAgaga                                                                                                      <  1:508506/149‑1 (MQ=255)
                                               ccGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCTCCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGAt                                                                                                     >  2:761136/1‑149 (MQ=255)
                                                ctgaaaTCGAGGCTGATGTATTTGAAATCACCGATATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCTCTTATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCTTCTCCTCAGGCGGGTATAGTTAAAGAGATc                                                                                                    <  2:795740/144‑1 (MQ=25)
                                                     aTCGGAGCTGATGAAGTTGAAATCACCGATATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCAAACAGTCGCTGATCACAATACAAGTCGACAAAGCCTCTATGGAAGTTGCGTCGACGCAGGCAGGTAACGTTAGATAGATCATAgg                                                                                               >  2:913756/1‑148 (MQ=255)
                                                       cGGGGCGGATGCAGTGGAAATCACCGAGCTCCTGGTCAAAGAGGGCGACAAATTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGtct                                                                                             <  2:229564/149‑1 (MQ=25)
                                                            cTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGtt                                                                                        <  1:22578/149‑1 (MQ=255)
                                                             tgatgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGATGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTg                                                                                       <  2:604494/149‑1 (MQ=255)
                                                                tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGctt                                                                                                                                                <  1:569285/89‑3 (MQ=255)
                                                                tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGctt                                                                                                                                                >  2:569285/1‑87 (MQ=255)
                                                                tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTAt                                                                                                                                            <  1:891823/93‑1 (MQ=255)
                                                                tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTAt                                                                                                                                            >  2:891823/1‑93 (MQ=255)
                                                                     ttGAACTCAACGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATaaa                                                                               <  2:908629/149‑1 (MQ=255)
                                                                     ttGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGACGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGTTaaa                                                                               >  2:370732/1‑149 (MQ=255)
                                                                        aaaTCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAcc                                                                            >  2:992289/1‑149 (MQ=255)
                                                                           tCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGAt                                                                                                     <  2:665346/121‑1 (MQ=255)
                                                                           tCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAg                                                                         >  2:289589/1‑149 (MQ=255)
                                                                           tCACCGAGATCCTGGGCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGAt                                                                                                     >  1:665346/1‑121 (MQ=255)
                                                                              ccGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGAcc                                                                      >  1:731626/1‑149 (MQ=255)
                                                                                         gTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTTGCGATAAAACCCAGACCGGCGCACTGAt                                                           <  2:846711/149‑1 (MQ=255)
                                                                                            aaaGTGGGCGACAAAGATGAAGCCGAACAGTCGCTGAGCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCACAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTAt                                                        <  2:940340/149‑1 (MQ=255)
                                                                                              aGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATgt                                                      >  1:480827/1‑148 (MQ=255)
                                                                                                  ggCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAACACCCAGACCGGCGCACTGATTATGAtttt                                                  >  1:231198/1‑149 (MQ=255)
                                                                                                      aCAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTACGTCTCCGCACGCGCGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGAt                                              >  2:640887/1‑149 (MQ=255)
                                                                                                                aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACg                                    <  2:505204/149‑1 (MQ=255)
                                                                                                                 gCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGCTCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACgg                                   >  1:176367/1‑149 (MQ=255)
                                                                                                                      aCAGTCGCTGATCACCGTAGAAGGCGACAAACCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAATCCCAGACCGGCGCACTGCTTATGATTTTCGATTCCGCCGACGGTgcag                              <  2:272647/149‑1 (MQ=255)
                                                                                                                          tCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGa                          <  1:957584/149‑1 (MQ=255)
                                                                                                                          tCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATGATGATTTTCGATTACGCCGACGGTGCAGCAGa                          >  1:481225/1‑149 (MQ=255)
                                                                                                                          tCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGGCTCTGTTGGCGATAAAACCCAGACCTGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGa                          <  2:489375/149‑1 (MQ=255)
                                                                                                                                aTCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGCTCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGc                    <  1:936416/149‑1 (MQ=255)
                                                                                                                                aTCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCACCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAACCCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGc                    <  1:107519/149‑1 (MQ=255)
                                                                                                                                 tCACCGTAGAAGGCGACAAAGCCTCGATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAACCCCCAGCCCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGCCGCTGCa                   >  1:327171/1‑149 (MQ=39)
                                                                                                                                  cACCGGAGAAGGCGACAAAGCCGCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAACGAGATCAAAGGCTCTGATGGCGATAAAACCCAGACCGCCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAc                  <  1:865600/149‑1 (MQ=255)
                                                                                                                                   aCCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGGCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAcc                 <  1:743863/149‑1 (MQ=255)
                                                                                                                                    ccGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCt                >  1:91774/1‑149 (MQ=255)
                                                                                                                                                aCAAAGCCTCTATGGAAGTACCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGGCTCTGTTGGCGCTAAAACCCAGACCGCCGCACTGATTATGATTTTCGATACCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaa    <  1:168222/149‑1 (MQ=255)
                                                                                                                                                  aaaGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCAATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaaga  <  2:102486/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGA  >  NC_000913/125669‑125963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑