Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,323,581 C→T 6.4% R45R (CGC→CGT)  atoD → acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,323,5810CT 6.4% 165.6 / 2.5 63R45R (CGC→CGT) atoDacetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (28/30);  new base T (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTG  >  NC_000913/2323433‑2323717
                                                                                                                                                    |                                                                                                                                        
aaGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGTGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTcgc                                                                                                                                          <  2:381094/149‑1 (MQ=255)
aaGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTcgc                                                                                                                                          <  1:589054/149‑1 (MQ=255)
  gagGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGTGa                                                                                                                                        >  2:927247/1‑149 (MQ=255)
  gagGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGTGa                                                                                                                                        >  2:923485/1‑149 (MQ=255)
  gagGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGa                                                                                                                                        >  2:483343/1‑149 (MQ=255)
    gtgaaaaaaaaTGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGGTGAAGCATTACTGGAATCTGGTGTTCGCGAcc                                                                                                                                      <  2:227460/144‑1 (MQ=255)
      gATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGATTCTGGTGTTCGCGACCTg                                                                                                                                    >  2:828670/1‑149 (MQ=255)
           aaaaTGAAAACAAAATTGATGACATTACACGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCCGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGATGAATTACTGGTATCTGGTGTTCGCGCCCTGACAtt                                                                                                                               >  1:982104/1‑149 (MQ=255)
           aaaaTGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGTGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACAtt                                                                                                                               <  1:779621/149‑1 (MQ=255)
              aTGAAAACAAAATTGATGCCATTACAAGACGCCTCCGGCTTCTTTCGTGACGGGAGGAGCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTGGCGCCCTGCCATTgct                                                                                                                            >  2:885944/1‑147 (MQ=255)
              aTGAAAACAAAATTGATGACCTTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGAt                                                                                                                            <  2:227201/149‑1 (MQ=255)
              aTGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGAt                                                                                                                            <  1:546746/149‑1 (MQ=255)
               tGAAAACAAAATTGATGACATTACAAGCCGCCACCGTCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCACGCCTGGTTGAAGCATTACTGGAATCTGGGGTTCGCGACCTGACATTGATa                                                                                                                           <  1:708584/149‑1 (MQ=255)
                gAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCAAGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAg                                                                                                                          <  2:246405/149‑1 (MQ=255)
                    aCAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCaa                                                                                                                      <  2:984010/149‑1 (MQ=255)
                             ctgaCATTACAAGAAGCCACCTGCTTCTTTAGTGACGGCATGACCATCATGGTGGGCGGATTTATGTGGATTGTCACTCCATCCGGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCTACCTGACATTGATAGCCAATGATACcgc                                                                                                             <  1:48373/148‑1 (MQ=255)
                                     aCAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTttgttg                                                                                                     >  2:769208/1‑149 (MQ=255)
                                      cAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGa                                                                                                    >  2:929848/1‑149 (MQ=255)
                                      cAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGGGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGa                                                                                                    <  2:493038/149‑1 (MQ=255)
                                       aaGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGAt                                                                                                   >  2:969644/1‑149 (MQ=255)
                                            gCCACCGGCTTCTTACGTGACGGCATGACCATCAAGGTGGTCGGATTTATGGGGAATGGCACTCCATCCCGCCTGGTTGAAGCATAACTGGAATCTGGTGTTCGCGAACTGACATTGAGAGCCAATGATACAGCGTTTCTTGCTACCgg                                                                                              >  2:849528/1‑149 (MQ=255)
                                             ccACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGc                                                                                             >  2:233133/1‑149 (MQ=255)
                                               cccGGCTTCTTGTGTGACGGCTTGACCATCATTGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCAt                                                                                           <  1:1014040/148‑1 (MQ=255)
                                               aCCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCAt                                                                                           >  2:318503/1‑149 (MQ=255)
                                               aCCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGCGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTGTGTTGATACCGGCag                                                                                           >  1:222078/1‑148 (MQ=255)
                                               aCCGGCTTCTTTCGTGACGGCATGACCATCATGCGCGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGACTCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCAt                                                                                           <  2:705859/149‑1 (MQ=255)
                                                ccGGCTTCTTTCGTGACGGCATGACCATCCTGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATc                                                                                          <  2:129657/149‑1 (MQ=255)
                                                       cttTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATACCCAATGATACCGCGTTTGTTGATACCGGCATCGGACCGc                                                                                   >  2:597562/1‑149 (MQ=255)
                                                        tttCGTGACGGCATGACCATCATGTGGGGCGGATTTATGGGGCTTGGCACTCCATCCCGCCGGGGTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCt                                                                                  <  1:596301/149‑1 (MQ=255)
                                                        tttCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTTGCACTCCAGCCCTCCTGGTTGCAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGATTGTTGATACCGGCATCTGTCCGCt                                                                                  >  2:934363/1‑149 (MQ=255)
                                                            gTGACGGCTTGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTctgc                                                                              >  1:29550/1‑146 (MQ=255)
                                                              gACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGt                                                                            <  1:828670/149‑1 (MQ=255)
                                                              gACGGCAGGTCCATCATGGTGGGCGGTTTTATGGGGATTGGCACTCCATCCGGCCGGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGt                                                                            <  1:444634/149‑1 (MQ=255)
                                                                cGGCATGACCATCATGGTGGGCGGATTTATGGGGATAGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCTGCATCGGTCCGCTCATCGTCa                                                                          <  2:101368/149‑1 (MQ=255)
                                                                   cATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGCCCTGACATTGATAGCGAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATg                                                                       >  1:686332/1‑149 (MQ=255)
                                                                     tGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGt                                                                     <  2:253098/149‑1 (MQ=255)
                                                                      gACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTc                                                                    <  1:642470/149‑1 (MQ=255)
                                                                        ccATCTTGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGAGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATAGTCGa                                                                  <  1:947423/149‑1 (MQ=255)
                                                                          atcatGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGt                                                                >  2:592088/1‑149 (MQ=255)
                                                                             ctggtggGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTCCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCg                                                             <  2:493995/148‑1 (MQ=255)
                                                                                 tggGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGTAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCaaa                                                         <  1:628849/149‑1 (MQ=255)
                                                                                     ccgATTTATGGCTATTGGCACTCCAACCCGCCTGGTTGAAGCATTACAGGAATCTGGTGATCGCGACCTGACATTGATAGCCAAAGATACCGCCTTTGTTGATACCGGCATCGGTCCGCTCAACCTCAATGCTCGAGTCCGCAAAGtca                                                     >  2:882472/3‑147 (MQ=255)
                                                                                         tttATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGc                                                 <  1:551904/149‑1 (MQ=255)
                                                                                           tATGGGGATGGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGCGCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCAGCGGTCCGCTCATCGTCAATGGTCGAGGCCGCAAAGTGATTGCtt                                               <  2:594046/149‑1 (MQ=255)
                                                                                           tATGGGGAGTGGCACTCCATCCCGCCTGGTGGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATCCCGGCAGCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCtt                                               <  1:1016586/149‑1 (MQ=255)
                                                                                             tGGGGATTGGCACTCCATCCCGCTTGGTTGAAGCATTACTGTAATCTGGTGTTCGCGACCTGACTTTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTca                                             <  1:572395/149‑1 (MQ=255)
                                                                                             tGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTca                                             <  1:891039/149‑1 (MQ=255)
                                                                                             tGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTca                                             <  1:318503/149‑1 (MQ=255)
                                                                                              ggggATTGGCACTCCATCCCGCCTGTTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCAGCTCATCATCAATTGTCGAGTCCGCAAAGTGATTGATTcac                                            >  2:548894/1‑149 (MQ=255)
                                                                                              ggggATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCCTCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTcac                                            >  2:288978/1‑149 (MQ=255)
                                                                                                ggATTGGCACGCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACat                                          <  2:317860/149‑1 (MQ=255)
                                                                                                  aTTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGTGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACatat                                        <  1:927247/149‑1 (MQ=255)
                                                                                                  aTTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGTGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACatat                                        <  1:923485/149‑1 (MQ=255)
                                                                                                       cACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTAGTTGATACCGGCATCGGACCGCTCATCGACAATGTTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCa                                   >  2:937494/1‑149 (MQ=255)
                                                                                                                ccGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCgg                          >  2:483577/1‑149 (MQ=255)
                                                                                                                ccGCCTCGTTGAAGCATTACTGTAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACGGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGGAAAGTGCTTGCTTCAAATAGCGGCACCAACCCgc                          >  2:583036/1‑148 (MQ=255)
                                                                                                                    cTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAAc                      <  1:483343/149‑1 (MQ=255)
                                                                                                                        ttGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGt                  >  1:112450/1‑149 (MQ=255)
                                                                                                                           aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCgg               >  1:928807/1‑149 (MQ=255)
                                                                                                                              cATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGgcgc            <  1:969644/149‑1 (MQ=255)
                                                                                                                               aTTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCa           >  1:776439/1‑149 (MQ=255)
                                                                                                                                    tGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGata      >  2:728338/1‑149 (MQ=255)
                                                                                                                                       caTCTGGTGTTCGCGAACTGACATTGATAGCCAATGATACCGTGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCACTGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCt   <  2:222078/148‑1 (MQ=255)
                                                                                                                                        ttCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGGCGGCGCATGATATCTg  >  2:838702/2‑149 (MQ=255)
                                                                                                                                        aTCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTg  >  2:1022369/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
AAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTG  >  NC_000913/2323433‑2323717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑