Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,352,779 G→A 6.1% G45S (GGT→AGT)  glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,352,7790GA 6.1% 174.8 / 2.3 65G45S (GGT→AGT) glpAanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (28/33);  new base A (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTA  >  NC_000913/2352632‑2352926
                                                                                                                                                   |                                                                                                                                                   
ttCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCgg                                                                                                                                                    >  1:716876/1‑149 (MQ=255)
    gagGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGcc                                                                                                                                                >  2:273947/1‑149 (MQ=255)
         aTAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGACGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCgg                                                                                                                                           >  1:615518/1‑149 (MQ=255)
           aaCAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGAGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGc                                                                                                                                         <  1:57878/149‑1 (MQ=255)
             cAATGAAAACTCGCGACTCGCAATCAAGTGCCGTGATTATCATTGGCGGCGGCGCAACGGGTGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACAACGCAACCAGTGCCACCGGGCGt                                                                                                                                       >  1:940107/1‑149 (MQ=255)
             cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCAGTGCCACCGGGCGt                                                                                                                                       >  1:683790/1‑149 (MQ=255)
              aaTGAACCCTCGCGACTCGCAATCCCGTGACGTGATTATCATTGGCGGCGGCGCAACGGGCGCCGGGCTTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTa                                                                                                                                      <  1:610596/149‑1 (MQ=255)
               aTGAAAACTCGCTAAGCGCAATCAAGTGACGTGATAAGCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCACTGCGCGGGGTGCGCGTGATGTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTaa                                                                                                                                     <  2:675241/149‑1 (MQ=255)
                agAAACCTCGCGACTCTCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCTGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAAc                                                                                                                                    <  1:123861/148‑1 (MQ=255)
                   aaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGAATGTGCCCTGCGCGGGCTGCGCGTGATTGTGGTTGAGCGCCACGCCATCGCAACCGGTGCCACCGGGCGTACCCAc                                                                                                                                 >  2:86382/1‑149 (MQ=255)
                         gcgACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCctg                                                                                                                           >  2:316069/1‑149 (MQ=255)
                          cgACTCGCAATCAAGTGACGTGCTTATCCTTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGGTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCctgc                                                                                                                          <  1:276910/149‑1 (MQ=255)
                                   aTCACGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCg                                                                                                                 <  1:567787/149‑1 (MQ=255)
                                    tCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACCTCGCAACCGGTGCCCCCGGGCGTCACCACGGCCTGCTGCACAGCgg                                                                                                                >  1:124376/1‑149 (MQ=255)
                                         tGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTgcgc                                                                                                           >  1:13594/1‑149 (MQ=255)
                                           aCGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTgcgcgc                                                                                                         >  1:529145/1‑149 (MQ=255)
                                             gTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACCGCGGTGCGCGCTa                                                                                                       >  2:781817/1‑149 (MQ=255)
                                              tGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCGACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTAt                                                                                                      <  2:979789/149‑1 (MQ=255)
                                               gATTATCATTGCCGGGGGCGAAACGGGAGCCTGGATAGCCCGCGACTGTTCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCGAGCGGGCGTAACCACGGCCTGCGGCACAGCGGTGCGCGCTATg                                                                                                     <  2:72200/149‑1 (MQ=255)
                                                 ttATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCg                                                                                                   >  2:856909/1‑149 (MQ=255)
                                                    acATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGGTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTa                                                                                                <  1:864189/148‑1 (MQ=255)
                                                      aTTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAAc                                                                                              <  2:616284/149‑1 (MQ=255)
                                                       ttGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAAcc                                                                                             >  1:410051/1‑149 (MQ=255)
                                                        tGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGGGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCg                                                                                            <  1:229170/149‑1 (MQ=255)
                                                          gcggcggcgCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGAt                                                                                          <  1:140639/149‑1 (MQ=255)
                                                                      cGGGAGCCGGGAGTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGGTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGcc                                                                              <  1:998542/149‑1 (MQ=255)
                                                                           gCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGa                                                                         >  1:715346/1‑149 (MQ=255)
                                                                            ccGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAAGCGATGCGGAATCGGCCCGCGaa                                                                        >  1:774486/1‑149 (MQ=255)
                                                                            ccGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGaa                                                                        <  2:417748/149‑1 (MQ=255)
                                                                             cGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAAt                                                                       <  1:317498/149‑1 (MQ=255)
                                                                                 aTTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGTGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGAGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCAt                                                                   <  1:559302/149‑1 (MQ=255)
                                                                                   tGCCCGCGACTGTGCGCTGCGCGGGCTGCGAGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTa                                                                 <  2:615518/149‑1 (MQ=255)
                                                                                     cccGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGt                                                               <  1:186781/149‑1 (MQ=255)
                                                                                     cccGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGt                                                               >  1:981749/1‑149 (MQ=255)
                                                                                         cgACTGTGCCCTGCGCTGGCGGCGCGTGATTTTGGTTGAGCTCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGaaa                                                           <  1:86382/149‑1 (MQ=255)
                                                                                               tgCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGACCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGa                                                     >  1:365413/1‑149 (MQ=255)
                                                                                                gCCCTGCGCGGGCTGCGCGAGATTTAGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGGGCGCGCTATGCGGTAAGCGATGCGGAATCGGCCCGCGAATGCATTAGTGCAAACCAGAt                                                    <  2:423449/149‑1 (MQ=255)
                                                                                                gCCCGGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGGGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGAt                                                    <  2:848271/149‑1 (MQ=255)
                                                                                                 cccTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGATTGCATTAGTGAAAACCAGATc                                                   >  2:888635/1‑149 (MQ=255)
                                                                                                 cccTGCGAGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGGCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATc                                                   <  1:891372/149‑1 (MQ=255)
                                                                                                   cTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCAGTAGTGAAAACCAGATCCt                                                 >  2:186184/1‑149 (MQ=255)
                                                                                                   cTGCGCGGGCTGCGCGTGATGTGGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCt                                                 <  1:311652/149‑1 (MQ=255)
                                                                                                                    gagcttGGTTGAGCGCCACGCCATCGCAACCGGCGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCAATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTc                                <  1:712884/145‑1 (MQ=255)
                                                                                                                       tttGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACt                             <  1:452662/149‑1 (MQ=255)
                                                                                                                           gTTGAGCGCCACGACATCTCAACCGGTGCCACCGGGCGTAAGAACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAATCCTGATCCTGAAACGCATTGCACGTCACTGCGt                         <  2:487842/149‑1 (MQ=255)
                                                                                                                           gTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGt                         <  1:409196/149‑1 (MQ=255)
                                                                                                                            ttGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGtt                        >  2:9831/1‑149 (MQ=255)
                                                                                                                            ttGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGtt                        >  1:567102/1‑149 (MQ=255)
                                                                                                                            ttGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGtt                        >  1:627335/1‑149 (MQ=255)
                                                                                                                              gAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCTTTAGTGAAAACCAGATCCTGAAACGCCTTGCACGTCACTGCGGTGa                      >  2:680447/1‑149 (MQ=255)
                                                                                                                               aGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGaa                     <  2:981749/149‑1 (MQ=255)
                                                                                                                                gctccACGACATCTCAACCGGTGACACCGGGCGATACCACGGCCTGCTGACCAGCGGTGCGCGCGATGCGTTAACCTCTGAGGAATCGGCCCGCGACTGCATTAGTGAAACCCAGATCCGGAACCGCATTGCACGTCACTGCGTTTaac                    <  2:425816/146‑1 (MQ=255)
                                                                                                                                  gcCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCATGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCATGAAACGCATTGCACGTCACGGCGTTGaccca                  >  2:479916/1‑149 (MQ=255)
                                                                                                                                    cACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGaaccaac                >  2:220713/1‑149 (MQ=255)
                                                                                                                                    cACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGaaccaac                <  2:216130/149‑1 (MQ=255)
                                                                                                                                    cACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGaaccaac                >  1:75113/1‑149 (MQ=255)
                                                                                                                                    cACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGaaccaac                <  1:894569/149‑1 (MQ=255)
                                                                                                                                          aaagCAACCGGTGCAACCTCGAGTAAACAAGGCCTGCTGAACACAGGTGCGCACTAAGCGGAACCCAATTCGGAATAGCCCCGCGCATGCATTAGTGTAAACCATAACCTGAAACGCATTGAACTTCACTGCGTTGAACCAACCAACgg          >  2:812437/4‑149 (MQ=255)
                                                                                                                                          aTCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCACGCGAATGCATTAGTGAAAACAAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAAAgg          >  2:102590/1‑149 (MQ=255)
                                                                                                                                             gCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCTGTAACCGATGCGGAATCTGCCAGCGAAAGCATTAGTGAAAACAAGATCATGAAACGCATTACACGTCACTGCGTTGAACCAAAAAACGGCCt       >  2:888656/1‑149 (MQ=255)
                                                                                                                                             gCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCAATAGTGAAACCCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACGCCCCAACGGCCt       >  2:803112/1‑149 (MQ=255)
                                                                                                                                               aaCCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGt     >  2:294974/1‑149 (MQ=255)
                                                                                                                                                aCCAGTGCCACCTGGGGTAAGCCCGGCCTGCTTCATAGCGGTGCGCGGTATTGGGTAACCGATGCGGAATGCGCCCGCGTATGGATTTGTGAAAACCAGATACTGTAACGCATTGCACGTCTCTGCGTTGAACCAACCAACGGCCTGtt    <  2:940107/149‑1 (MQ=255)
                                                                                                                                                aCCAGTGCCACCGGGCGTAACCACGGCCTGCTGCTCAGCGGTGCGCGCTATGCGGTATCCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGtt    <  2:683790/149‑1 (MQ=255)
                                                                                                                                                 ccGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGttt   <  1:123968/149‑1 (MQ=255)
                                                                                                                                                  cGGGGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTa  <  2:124376/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTA  >  NC_000913/2352632‑2352926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑