Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 126,060 A→G 5.2% E122E (GAA→GAG)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,0600AG 5.2% 221.4 / 2.1 78E122E (GAA→GAG) aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base A (31/42);  new base G (2/2);  total (33/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGG  >  NC_000913/125912‑126206
                                                                                                                                                    |                                                                                                                                                  
ttttCGATTCCGCCGACGGGGCAGCAGACGGAGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCCGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGaa                                                                                                                                                    <  1:583595/149‑1 (MQ=255)
   tCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATc                                                                                                                                                 <  2:385945/149‑1 (MQ=255)
      aTTCAGCCGACGGCGCAGACGACGCCGCAGCAGCGCAGGCAGCAGATAAGAAAGAAGCACCTCCTGCTGCAGCTGCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCTGAGACGAAGTTGAAGTGACTGaagtgatg                                                                                                                                              <  2:569041/149‑7 (MQ=16)
       ttCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGCCGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTgg                                                                                                                                             >  1:611524/1‑149 (MQ=255)
        tCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGt                                                                                                                                            <  1:992289/149‑1 (MQ=255)
            ccgACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGaaa                                                                                                                                        <  1:289589/149‑1 (MQ=255)
            ccgACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCGGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGaaa                                                                                                                                        <  2:507301/149‑1 (MQ=255)
            ccgACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCGCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTATCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGaaa                                                                                                                                        <  1:640887/149‑1 (MQ=255)
            ccgACGGTGCAGCAGACGCTGCACCTGCGCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGaaa                                                                                                                                        <  2:231198/149‑1 (MQ=255)
                 ggTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTgg                                                                                                                                   <  1:126413/149‑1 (MQ=255)
                  gTGCAGCCGACCCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCGCCTGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGc                                                                                                                                  <  2:481225/149‑1 (MQ=255)
                      agcagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACATTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATa                                                                                                                              >  2:423660/1‑149 (MQ=255)
                       gcagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGTTATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATaa                                                                                                                             <  1:370732/149‑1 (MQ=255)
                        cagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                                                                                            <  2:91774/149‑1 (MQ=255)
                         agACGCTTCACCTGCTCAGGCAGAAGAGAAGAAACCAGCAGCTCAGGCAGCAGCACCAGCGGCTGCGGCGGCATAAGACGTTAACGTTCCGGATATCGGCTGAGACGAAGTTGAAGGGACCGAAATCCTGGTGAAAGTTGGCGATAAAg                                                                                                                           <  1:913756/149‑1 (MQ=255)
                          gACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGCAGTTGCAGTGACCGAAATCCGGGTGAAAGTTGGCGATAAAGt                                                                                                                          >  2:154920/1‑149 (MQ=255)
                           aCGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt                                                                                                                         <  2:298628/149‑1 (MQ=255)
                           aCGCTGAACATGATCAGGGAGACTATAATATATAACCAGCTACTGCAGCATCACCATATTCTTCTTCGGCTATAGAAGTTAACGTTCCGGATATCGGCTGCGACGAAGTTGAACTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt                                                                                                                         <  1:836422/149‑1 (MQ=9)
                             gCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCCGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGGTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTCGTGAAAGTTGGCGATAAAGTTGa                                                                                                                       >  1:213715/1‑149 (MQ=255)
                              cTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa                                                                                                                      <  1:882939/149‑1 (MQ=255)
                                 cACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCt                                                                                                                   >  1:628613/1‑149 (MQ=255)
                                  aCCTGCTCAGGCAGAAGAGAAGAAAGACGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                  >  1:891258/1‑149 (MQ=255)
                                  aCCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                  >  1:924295/1‑149 (MQ=255)
                                   ccTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa                                                                                                                 <  1:203813/149‑1 (MQ=255)
                                     tGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc                                                                                                               >  2:313347/1‑149 (MQ=255)
                                       cTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                                                                                             >  1:344298/1‑149 (MQ=255)
                                            gCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTAAACGTTCCGGATATCGGCAGCGACGAAGATGAAGTGACCGAAATCATGGTGAAAGATGGCGATAAAGATGAAGCTGAACAgaagct                                                                                                        >  2:890505/1‑144 (MQ=255)
                                               gaagagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGAt                                                                                                     >  1:8884/1‑149 (MQ=255)
                                                aagagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc                                                                                                    <  2:899383/149‑1 (MQ=255)
                                                  gagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAACACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAc                                                                                                  >  2:868621/1‑149 (MQ=255)
                                                    gcagaaagcaGCAGCGCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGCAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCg                                                                                                <  2:666429/147‑1 (MQ=255)
                                                     aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt                                                                                               >  1:144026/1‑149 (MQ=255)
                                                     aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTGCCGGAGATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt                                                                                               <  1:963085/149‑1 (MQ=255)
                                                          agaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg                                                                                          >  1:541299/1‑149 (MQ=255)
                                                           gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAgg                                                                                         <  2:203140/149‑1 (MQ=255)
                                                            aagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                                                                                        <  1:978747/149‑1 (MQ=255)
                                                               cagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc                                                                                     >  2:378963/1‑149 (MQ=255)
                                                                agcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGAAGCGACGAAGTTGAAGTGACCGAAATCATGGTGAAATTTGGCGATAAAGTTGACGCTGAACACTCGCTGATCACCGTAGAAGgagaaa                                                                                    >  2:670595/1‑147 (MQ=25)
                                                                    ccGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGc                                                                                >  2:307784/1‑149 (MQ=255)
                                                                      ggCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt                                                                              <  1:246728/149‑1 (MQ=255)
                                                                            cgcaCCAGCGGCTGCGGCGGCAAACGACGTTCACGTTCCGGATATCGGCAGCGCCGAAGTGGCAGTGACCGAAATCCTGGGGAAAGTTGGCGATAACGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATgg                                                                        <  1:154920/148‑1 (MQ=25)
                                                                            cgaaCCAGCGGCTGGGTCGGCAAAAGACGTTAACGTTCCGGATCTCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg                                                                        <  2:210922/146‑1 (MQ=255)
                                                                              caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGCCGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATAACCGTAGAATGAGACAATTCTTCTATGGaa                                                                      >  1:57870/1‑149 (MQ=21)
                                                                              caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa                                                                      <  2:319986/149‑1 (MQ=255)
                                                                                ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt                                                                    <  2:801983/149‑1 (MQ=255)
                                                                                  aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTc                                                                  <  1:299594/149‑1 (MQ=255)
                                                                                   gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTcc                                                                 >  2:917356/1‑149 (MQ=255)
                                                                                   gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGAGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGTAAGTTcc                                                                 >  2:561428/1‑149 (MQ=255)
                                                                                    cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCg                                                                <  1:966812/149‑1 (MQ=255)
                                                                                         gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc                                                           <  2:855034/149‑1 (MQ=255)
                                                                                           ggcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGt                                                         <  1:868621/149‑1 (MQ=255)
                                                                                               gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGc                                                     <  1:478807/149‑1 (MQ=255)
                                                                                                         ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg                                           >  2:348526/1‑149 (MQ=255)
                                                                                                            aCGTTACGGTTATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAATGTTGAAGCTGAACAGTCGCTGTTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTGCGTTTGCTGGCACCGTGaaa                                        <  1:890505/149‑1 (MQ=255)
                                                                                                               ttCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag                                     <  2:611524/149‑1 (MQ=255)
                                                                                                                tCCGGATATCGGCAGCGACGAAGATGAAGTGAACGAAAGCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga                                    <  1:391583/149‑1 (MQ=255)
                                                                                                                 ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt                                   <  2:8884/149‑1 (MQ=255)
                                                                                                                 ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt                                   <  2:924295/149‑1 (MQ=255)
                                                                                                                     atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATTGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaaa                               >  2:957447/1‑149 (MQ=255)
                                                                                                                        tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGTGATCAAAGTg                            <  1:980505/149‑1 (MQ=255)
                                                                                                                           gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAAc                         >  1:755980/1‑149 (MQ=255)
                                                                                                                             aGCGACGAAGTTGAAGTGCCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                       >  2:24147/1‑149 (MQ=255)
                                                                                                                             aGCGACGAAGTAGAAGTGACCTAATTACTTGTTAAAGTAGGCGATAAAGGTGAAGCTGAACAGTCGCTGATCACCTTTGTTGGCGACAAGGCCTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt                       <  1:834060/149‑1 (MQ=255)
                                                                                                                               cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTgg                     <  2:541299/149‑1 (MQ=255)
                                                                                                                               cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTgg                     <  1:1016480/149‑1 (MQ=255)
                                                                                                                               cgacgaAGGTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGTACGTgg                     <  2:18617/149‑1 (MQ=255)
                                                                                                                                gacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGTTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                    >  1:202768/1‑149 (MQ=255)
                                                                                                                                gacgaAGGTGAAGTGACCGAAATCCTGGTGACAGTTGGCGATACAGGTGAAGCTGAACAGTCGCTGAGCACCGTCGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                    <  1:561428/149‑1 (MQ=255)
                                                                                                                                       ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACaaa             >  1:422359/1‑149 (MQ=255)
                                                                                                                                       ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACaaa             >  1:860623/1‑149 (MQ=255)
                                                                                                                                         gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt           >  1:533497/1‑149 (MQ=255)
                                                                                                                                         gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt           <  1:423660/149‑1 (MQ=255)
                                                                                                                                         gAAATGACCGATATCCTGGTGAAAGTGGGCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt           <  1:952297/149‑1 (MQ=255)
                                                                                                                                              caCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa                                        <  2:812303/114‑1 (MQ=14)
                                                                                                                                              caCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa                                        >  1:812303/2‑115 (MQ=14)
                                                                                                                                               aCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGGCGTGAAAGACCTGAAAGTCAACGTTGGAGAGAAAgtgaaaac     >  2:733102/1‑144 (MQ=11)
                                                                                                                                                 cGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGTGAt                                   >  1:299478/1‑117 (MQ=14)
                                                                                                                                                 cGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGTGAt                                   <  2:299478/117‑1 (MQ=14)
                                                                                                                                                 cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAATTGAACGTGGGTGACAAAGTGTCTACCg   >  1:171324/1‑149 (MQ=255)
                                                                                                                                                  gAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg  >  1:212057/1‑149 (MQ=255)
                                                                                                                                                   aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                                                <  2:675540/70‑1 (MQ=255)
                                                                                                                                                   aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                                                >  1:675540/1‑70 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGG  >  NC_000913/125912‑126206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑