Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,357,027 C→T 9.2% L42L (CTG→TTG)  yfaD → transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,357,0270CT 9.2% 173.9 / 7.9 65L42L (CTG→TTG) yfaDtransposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base C (33/26);  new base T (3/3);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

ATATCAGGTGACGGACAACCATGACCGAATCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATAT  >  NC_000913/2356884‑2357175
                                                                                                                                               |                                                                                                                                                    
atatCAGGTGACGGACAACCATGACCGAATCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACAGCGCAAGCTTTGCAACCTGCaa                                                                                                                                                 >  1:973308/1‑149 (MQ=255)
  atCAGGTGACGGACAACCATGACCGACTCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAAc                                                                                                                                               <  1:672673/149‑1 (MQ=255)
     cggTGACGGACAACCATGACCGTATCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGTAAGGTTTGCAGGTTGCAAAGCtt                                                                                                                                            <  1:787604/148‑1 (MQ=255)
     aGGTGACGGACAACCATGACCGAATCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAGGTTGCAAAGCtt                                                                                                                                            <  2:358401/149‑1 (MQ=255)
     aGGTGACGGACAACCATGACCGAATCAACAACCTCCTCCCCGCATCATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAGGTTGCAAAGCtt                                                                                                                                            <  2:294013/149‑1 (MQ=255)
       gTGACGGACAACCATGACCGAATCACGAACCTCCTCCCCGCATCATGCGGAAATTAAAACCTATATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGCCCCACTTCTCAAGCTTGGCAACCTGCTAACCTTAc                                                                                                                                          >  1:694519/1‑149 (MQ=255)
        tGACGGACAACCATGTCCGAATCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCTCGGGATTTTCTCGATATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCATACCTTACg                                                                                                                                         >  1:475621/1‑149 (MQ=255)
         gACGGACAACAATGACCGAATCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCCCGGGAGTTTCTCGCAATACATTGACCAGAACCACTGCGCAATCTTTGAACCCTGCAAACCTTACGc                                                                                                                                        >  1:618629/1‑149 (MQ=255)
                           aaTCAACAACCTCCTCCCCGCATGATGCGGTATTTAACACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCCAACCTTACGCCTGGAACCCACTAGtttt                                                                                                                      >  2:810802/1‑149 (MQ=255)
                             tCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTAt                                                                                                                    >  1:899410/1‑149 (MQ=255)
                             tCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTAt                                                                                                                    >  1:903048/1‑149 (MQ=255)
                             tCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACGAGTTTTAt                                                                                                                    >  1:151028/1‑149 (MQ=255)
                                 caacCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTATATGGaa                                                                                                                >  1:862505/1‑149 (MQ=255)
                                  aacCTCCTCCCCGCATGATGCGGTATTTAAAACCTATATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACGAGAACCACTGCGCAAGCTTTGCAACCTGCTAACCTTACGCCTGGCACCAACTAGTTTTATTGaaa                                                                                                               >  2:200508/1‑149 (MQ=255)
                                   acCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGaaaa                                                                                                              >  1:331279/1‑149 (MQ=255)
                                     ctcctcCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGaaaaaa                                                                                                            >  1:20821/1‑149 (MQ=255)
                                      tcctccCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAg                                                                                                           <  1:103072/149‑1 (MQ=255)
                                        ctccCCGCATGATGCGGTATTTAAAATCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGtt                                                                                                         <  2:86656/149‑1 (MQ=255)
                                        ctccCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGCATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGACCCCACTAGTTTTATTGAAAAAAGtt                                                                                                         >  2:250917/1‑149 (MQ=255)
                                                atgatgCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCt                                                                                                 <  1:988796/149‑1 (MQ=255)
                                                  gatgCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTta                                                                                               <  2:357305/149‑1 (MQ=255)
                                                  gatgAGGTATTTAAAACCTTTATGTTCACACCCGTAACCGCACGGGATTTTCTCGAAAAACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTTCAATCCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTta                                                                                               <  1:238175/149‑1 (MQ=255)
                                                         tATTTAAAACCTTTATGTTCACACCCGAAACCGCCCGGGATTTTCTCGAAATACATTTACCCGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCGGGAACCCACTCGTTTTATTGCAAACAGTTTACGCGCTTACTACTCg                                                                                        <  2:74404/149‑1 (MQ=255)
                                                            ttAAAAGATTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGAt                                                                                     <  1:329220/149‑1 (MQ=255)
                                                             tAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGCAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATg                                                                                    <  1:542655/149‑1 (MQ=255)
                                                               aaaCCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGtt                                                                                  >  2:13841/1‑149 (MQ=255)
                                                                   cTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTtgt                                                                              >  1:151346/1‑149 (MQ=255)
                                                                    tttATGTTCACACCCGAAACCGCACGTGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGAGCTTACTACTCGGATGTTTtgtg                                                                             <  2:57036/149‑1 (MQ=255)
                                                                         gTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCg                                                                        >  1:606391/1‑149 (MQ=255)
                                                                               acCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGACCCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTGTTGTGGTCCGTGGaaa                                                                  >  2:432689/1‑149 (MQ=255)
                                                                               acCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTAATCGGATGTTTTGTGGTCCGTGGaaa                                                                  >  1:659291/1‑149 (MQ=255)
                                                                                 ccGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAAcc                                                                >  1:580184/1‑149 (MQ=255)
                                                                                 ccGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAAcc                                                                >  2:488266/1‑149 (MQ=255)
                                                                                 ccGAAACCGCACGGGATTTTCTCGAAATACATTAACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTAATTGAAAAACTTTTAAGCGCTAACTACACGGATGTTTTGTGGTCCGTGGAAAcc                                                                >  1:945411/1‑149 (MQ=255)
                                                                                        cGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACg                                                         >  1:889670/1‑149 (MQ=255)
                                                                                        cGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACg                                                         <  2:331178/149‑1 (MQ=255)
                                                                                          cACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGt                                                       <  1:72383/149‑1 (MQ=255)
                                                                                             gggATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGAc                                                    <  2:919410/149‑1 (MQ=255)
                                                                                                aTTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGc                                                 <  2:296992/149‑1 (MQ=255)
                                                                                                 ttttCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCt                                                <  1:520039/149‑1 (MQ=255)
                                                                                                  tttCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCta                                               >  1:200094/1‑149 (MQ=255)
                                                                                                            tACATTTACCAGAACCACTGCGCAAGCTTTGCACACTGCAAAACTTACGCCTGGAACCCACTATGTTAATTGAAAAAAGTTTACTCGCTTACTACTCGTATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGTCTATATCTACTGc                                     <  2:618629/149‑1 (MQ=255)
                                                                                                               aTTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTg                                  >  1:289849/1‑149 (MQ=255)
                                                                                                                tttACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGAGGGTGACGGCTATATCTACTGAGTGa                                 >  1:336935/1‑149 (MQ=255)
                                                                                                                 ttACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGAt                                <  2:899410/149‑1 (MQ=255)
                                                                                                                 ttACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCATGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGAt                                <  2:903048/149‑1 (MQ=255)
                                                                                                                     cAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGaa                            >  2:500596/1‑149 (MQ=255)
                                                                                                                     cAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGaa                            <  1:409511/149‑1 (MQ=255)
                                                                                                                      aGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAAc                           >  1:52047/1‑149 (MQ=255)
                                                                                                                           cACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCaa                      >  2:7343/1‑149 (MQ=255)
                                                                                                                             atGCGCAATCTTTGCAACCTGCAAACCTTACGCCTGGTACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTAGTCGGATGTTTTGTGGTCCTTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAAGATCAAAg                    <  1:594326/148‑1 (MQ=255)
                                                                                                                                 gtaaGGTTTGCAGGTTGCAAAGCTTGCGCCTGGAACCCACTTGATTTATTGAAAAAAGTTTACGCGCTTACTACTCGGAAGTTTTGTGGTCCGTGGAAACCAGCCACGGTGCCGGCTATATCTACTGCGTGATTGACCATCAAAGctcg                >  2:318224/3‑148 (MQ=255)
                                                                                                                                 gtaaGGTTTGCAGGTTGCAAAGCTTACGCCTGGATCCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGTCTATATCTACTGCGTGATTGAACATCAAAGctct                >  1:540245/3‑149 (MQ=255)
                                                                                                                                 gcAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATCTGTTGTGGTCCGTGGAAACCAGCGACGGAGACGGCTATATCTACTGCCTGATTTAACAACAAAGctct                >  2:385423/1‑149 (MQ=255)
                                                                                                                                   aaGGTTTGCAGGTTGCAAAGCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGc              >  2:639968/1‑149 (MQ=255)
                                                                                                                                   aaGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGc              >  2:373213/1‑149 (MQ=255)
                                                                                                                                   aaGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGc              >  1:638388/1‑149 (MQ=255)
                                                                                                                                    aGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCa             <  1:242488/149‑1 (MQ=255)
                                                                                                                                    aGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCa             <  2:889670/149‑1 (MQ=255)
                                                                                                                                      cTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGa           >  1:16257/1‑149 (MQ=255)
                                                                                                                                         tGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGaaaa        <  1:736469/149‑1 (MQ=255)
                                                                                                                                         tGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGaaaa        <  2:151346/149‑1 (MQ=255)
                                                                                                                                           cAACCTGCAAACCTTACGCCTGGACCCCACTAGTTTTAGTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGa      >  2:532/1‑149 (MQ=255)
                                                                                                                                            aaCCTGCAAACCTTACGCCTGGAACCCACTTGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTATTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGaa     <  2:973308/149‑1 (MQ=255)
                                                                                                                                             aCCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAat    <  2:221596/149‑1 (MQ=255)
                                                                                                                                               cTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGGGACGGCTATATCTACGGGGGGATGGAACATCAAAGCTCTGCAGAGAAGAatat  >  2:969685/1‑149 (MQ=255)
                                                                                                                                               cTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGCGGAACCCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAatat  >  2:989768/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
ATATCAGGTGACGGACAACCATGACCGAATCAACAACCTCCTCCCCGCATGATGCGGTATTTAAAACCTTTATGTTCACACCCGAAACCGCACGGGATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATAT  >  NC_000913/2356884‑2357175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑