| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,357,665 | G→A | 9.1% | G254G (GGG→GGA) | yfaD → | transposase_31 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,357,665 | 0 | G | A | 9.1% | 120.3 / 2.2 | 55 | G254G (GGG→GGA) | yfaD | transposase_31 family protein |
| Reads supporting (aligned to +/- strand): ref base G (19/29); new base A (2/3); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAG > NC_000913/2357521‑2357806 | cagccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATc < 1:790724/149‑1 (MQ=255)cagccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTAATGAGGAGATTGGCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAACGGCATc < 1:797369/149‑1 (MQ=255) gccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGATCGGCTCCGGACGGAAGGGCaggaa > 1:351737/1‑145 (MQ=255) ccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGCTTGCCGAACGTTCACCACTACAAAAGTAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCaat > 2:756415/1‑148 (MQ=255) agcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAAtt < 1:941343/149‑1 (MQ=255) cTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCAACAAATtgg > 1:670145/1‑149 (MQ=255) aCAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggct < 2:727847/149‑1 (MQ=255) aCAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACATCCCGTTTCAGACGTTTTATTGAGGAGATTGCCTAACGTTCTCAACTAAAAAAGGAGAGATTAATGACTATTGCAGAACGGATACTGAATGAATGAAATCAAATtgaat > 1:558107/1‑146 (MQ=255) aaaCACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggctgg < 2:657799/149‑1 (MQ=255) acacTGTTAATTTATCTGCTGCAATGCTGCGATACCTCCCGTTTCACCCTTTTTATTGAGGAGATTGCCGAACTTTCACCACTACAAAAGTAGAGATTAATGACTATTGCTGAACGGCTAAGGCAGGAAGGGCATCAAATTGGCTGGCa < 2:804714/149‑1 (MQ=37) gTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAg < 2:407169/149‑1 (MQ=255) tttAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAgg > 1:535028/1‑149 (MQ=255) tttAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGAGTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAgt > 1:203291/1‑148 (MQ=255) cttccgaTCTGCTGCATTCCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGACCAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGt < 2:223667/143‑1 (MQ=25) tAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTa > 1:746231/1‑149 (MQ=255) tAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACAACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTa > 2:932668/1‑149 (MQ=255) ttATCTGCTGCAATGCGGCGATACCTCACGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGc < 1:78337/149‑1 (MQ=255) aTCTGCTGCAATGCGGCGATACCGCCCGTTTCACCCGTTTAATTTAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaatta < 2:746231/149‑6 (MQ=21) gctgcAATGCGGCGATACCTCCCGTTTAACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGTGCATCAAATTGGCTGGCAGGAAGGTATGCATGAAc > 1:883120/1‑149 (MQ=255) ctgcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGAATGAACa > 2:177158/1‑149 (MQ=255) cAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑Gc < 2:24746/149‑1 (MQ=255) aaTGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑Gcc < 1:266356/149‑1 (MQ=255) cggcgATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGACCGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTa < 1:24813/149‑1 (MQ=255) cggcgATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTa > 2:966550/1‑149 (MQ=255) ggcgATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTaa > 2:380126/1‑149 (MQ=255) ggcgATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GAGATTaa > 2:622551/1‑149 (MQ=255) cgATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGAATGAACAATCCCATTaaa > 1:256102/1‑149 (MQ=255) cccGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGCCTATTGCTGCACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATTCATGAACAA‑GAAAATAAAAATGCATTg > 1:127750/1‑149 (MQ=25) cccGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATT‑AAATTGCTTTgc > 1:809195/1‑149 (MQ=255) aTTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAggg < 1:593899/149‑1 (MQ=255) aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGTTCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAC‑GCCATTAAAATTTCTTTGCGCATGCTGGAGCAGGGCttt < 2:883120/149‑1 (MQ=255) aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCCTCAAATAGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCttt < 2:252588/149‑1 (MQ=255) ggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTg > 1:469523/1‑149 (MQ=255) gaTTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAAc < 2:916186/149‑1 (MQ=255) aaCGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGAtt > 2:857403/1‑149 (MQ=255) aCGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCATTGATCGTGACATtg > 1:453160/1‑149 (MQ=255) accacTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTgg < 1:380126/149‑1 (MQ=255) ccacTACAAAAGGCGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGc < 1:338311/149‑1 (MQ=255) cacTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGTCATCAAATTGGCTGGCAGGAAGGTATGAATGAACAA‑GACATTAAAAATGCTATGCGAATGCTGGACCTCGTCTTTGAACGTGACATTGAGCTGGCg > 1:903386/1‑149 (MQ=21) tACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGCCa > 2:680331/1‑149 (MQ=255) cAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGACATCAAATTGGCTGGCa < 1:811986/65‑1 (MQ=14) cAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGACATCAAATTGGCTGGCa > 2:811986/1‑65 (MQ=14) agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAAATGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCAc < 1:563789/149‑1 (MQ=255) tAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGAt < 2:246544/149‑1 (MQ=255) aaTGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGAGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATg < 2:202322/149‑1 (MQ=255) tGACTATTGCTGACCGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTCTGCCTGACCCA‑GCCATTAACATTGCTATGCGCATGCTGGAGCAGGGCTTTGAACTTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCt < 1:698465/149‑1 (MQ=37) tGACTATGTATGCACGGCCCCGGCCGGCAGGGCCTCCACTTGGCGGGCAGGAAGGTATGCATGAACAA‑GCCATTAACATTGCTTTGCGCATGCTGTAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCt < 2:1021642/149‑1 (MQ=255) tGCTGACCGGCTACGGCATGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCTTGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCATGGCTTTGAACGTGAGATTGTGCTGGCGACAACCAAACTCACTGATGCTGATATTc < 1:847363/149‑1 (MQ=38) gAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGaa < 1:857403/149‑1 (MQ=255) gAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGGATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGaa < 1:858202/149‑1 (MQ=255) cTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCa < 2:203291/149‑1 (MQ=255) aCGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCAtt < 1:966550/149‑1 (MQ=255) cAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCGTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTaaaa < 1:756415/149‑1 (MQ=255) cAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATT‑AAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAg < 2:809195/149‑1 (MQ=255) aGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAg < 1:680331/149‑1 (MQ=255) | CAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAA‑GCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAG > NC_000913/2357521‑2357806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |