Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,432,990 G→C 6.6% intergenic (‑48/+22) folC ← / ← accD bifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,432,9900GC 6.6% 192.9 / 4.9 71intergenic (‑48/+22)folC/accDbifunctional folylpolyglutamate synthase/ dihydrofolate synthase/acetyl‑CoA carboxylase, beta (carboxyltransferase) subunit
Reads supporting (aligned to +/- strand):  ref base G (37/28);  new base C (3/2);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGA  >  NC_000913/2432842‑2433138
                                                                                                                                                    |                                                                                                                                                    
cgcTCACGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAg                                                                                                                                                      <  1:895539/149‑1 (MQ=255)
   tCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTgg                                                                                                                                                   >  1:790967/1‑149 (MQ=255)
      aGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccc                                                                                                                                                >  2:629195/1‑149 (MQ=255)
      aGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGGTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccc                                                                                                                                                >  1:833631/1‑149 (MQ=255)
       ggCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccct                                                                                                                                               >  2:489382/1‑149 (MQ=255)
        gCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctg                                                                                                                                              <  2:22482/149‑1 (MQ=255)
         ccGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTTCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGGGCCACTGgctctgc                                                                                                                                             <  1:570508/149‑1 (MQ=255)
          cGAGATCGATAGTTTTAATGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGgccctgcc                                                                                                                                            <  1:572904/149‑1 (MQ=255)
              aTCGATAGTTTTCCTGTGCAGGTGTTCCAGATAAGAAAGCCACTAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGCAATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCtta                                                                                                                                        <  2:147434/149‑1 (MQ=255)
               tCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAACGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTTAGAATAGCAAAAGGGCAGGGCCACTGGCTCTGCCCtttt                                                                                                                                       <  1:541042/149‑3 (MQ=255)
                cGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGAGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatc                                                                                                                                      >  2:821297/1‑149 (MQ=255)
                 gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCTCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                                     >  1:910076/1‑149 (MQ=255)
                 gATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatca                                                                                                                                     >  1:617059/1‑149 (MQ=255)
                  aTAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGTCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGTCCCTGCCGttatcag                                                                                                                                    >  1:691912/1‑149 (MQ=255)
                  aTAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcat                                                                                                                                    >  1:216120/1‑148 (MQ=255)
                  aTAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcag                                                                                                                                    >  1:393299/1‑149 (MQ=255)
                       tttACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatc                                                                                                                               <  2:617059/149‑1 (MQ=255)
                        ttAATGAGCAGGTTTTCCACTTAAGAAAGCCACCAAGGCAGAGGCGAAGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGATACGGTGATAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCttatcagttatca                                                                                                                              <  1:584424/149‑1 (MQ=255)
                            tgtgCATGTTTTCCAGATAAGAAAGCCACGAAGCCAGATGCGACGCGCCTAGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTATTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGcc                                                                                                                          >  2:254325/1‑149 (MQ=255)
                                    ttttCCAGATATGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGGGCGTTTGATAATCATGGTAGCCGCTGATTCGTTACGGTGAGAATAGCAAAAGCGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCCGGTTc                                                                                                                  <  1:691276/149‑1 (MQ=255)
                                      ttCCAGATAAGAAAGCCACGAAGCCAGAGGCGCCGCGGCTCGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCt                                                                                                                >  1:590930/1‑149 (MQ=255)
                                           gATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGTCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATcc                                                                                                           >  2:185846/1‑149 (MQ=255)
                                           gATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATcc                                                                                                           >  2:182490/1‑149 (MQ=255)
                                              aaGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCATGCCTCAGGTTCCTGATCCGGt                                                                                                        >  2:298742/1‑149 (MQ=255)
                                                gAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCCTGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCAGATCAGATAGCCGGCCTCAGGTTCCTGATCCGGTAc                                                                                                      >  1:557900/1‑149 (MQ=255)
                                                gAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTAc                                                                                                      >  1:109988/1‑149 (MQ=255)
                                                     ccACGACGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCgggg                                                                                                 >  1:135367/1‑149 (MQ=255)
                                                       ccGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTCCGGAGAGAATAGCAACAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGt                                                                                               <  1:568333/148‑1 (MQ=255)
                                                         gAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTAc                                                                                             <  1:555181/149‑1 (MQ=255)
                                                           aGCCAGAGGCGACGCGGCGTGAGGAGTGCGTTAGATAATCATGGTATCCGCTGATTCCTTACGGTGAGAATAGCAAAAGGGCAGCGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGATCCTGATCCGGTACCGGGGGTACCa                                                                                           <  2:286359/149‑1 (MQ=255)
                                                            gCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCAc                                                                                          >  1:460774/1‑149 (MQ=255)
                                                                  ggCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGcc                                                                                    >  2:123514/1‑149 (MQ=255)
                                                                   gCGACGCGGCTTGAGTAGTGCGTTTGATAATCATGGTATCCGAAGATTAGTTACGGTGAGAATAGCAAAAGGGCAGAGACAGTGGCCCTGACCTTAACAGTTATCAGGCCTCATGTTCCTGATCCGGTACCGGGGGTACCACTACGCCt                                                                                   >  1:110515/1‑149 (MQ=255)
                                                                   gCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGCGGGTACCACTACGCCt                                                                                   >  2:782827/1‑149 (MQ=255)
                                                                   ccGACGCGGCTTGAGGAGAGCGTTTGATAATCAAGGTATCCGCTGATTCGTTACGGAGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCt                                                                                   <  1:432171/148‑1 (MQ=255)
                                                                                aggagTGCGTTTGATAATCATGGTTTCAGCTGATTCGTTACGGTGAGAAAAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCtt                                                                      <  1:900163/149‑1 (MQ=255)
                                                                                  gagTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCa                                                                    >  2:596889/1‑149 (MQ=255)
                                                                                    gTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGGGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAgg                                                                  <  1:424395/149‑1 (MQ=255)
                                                                                     tGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGa                                                                 >  2:382731/1‑149 (MQ=255)
                                                                                               aaTCCTGGTATCCGCTGATTCGTTACGGGGAGAATAGCAAACGGGCCGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTGCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTg                                                       <  1:1002757/149‑1 (MQ=255)
                                                                                                aTCATGGTATCCGCTGATTCGTTACGGTGCGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCCGGTTCCTGTTCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTgg                                                      <  1:489382/149‑1 (MQ=255)
                                                                                                 tCATGGGAGCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGc                                                     <  1:852260/149‑1 (MQ=255)
                                                                                                  cATGGTATCTGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCa                                                    <  2:370847/149‑1 (MQ=255)
                                                                                                   aTGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAg                                                   >  2:771941/1‑149 (MQ=255)
                                                                                                    tGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGa                                                  >  2:801921/1‑149 (MQ=255)
                                                                                                     ggTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCGCAGGTTCCTGATCCGGGACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGAt                                                 <  2:1019138/149‑1 (MQ=255)
                                                                                                      gTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGAtt                                                >  2:915385/1‑149 (MQ=255)
                                                                                                           cGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATtcatca                                           >  1:961695/1‑149 (MQ=255)
                                                                                                                    gTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTACGCCa                                  >  2:69825/1‑149 (MQ=255)
                                                                                                                        cGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGCTCCGGTACCGGGGGTACCACTACGCCTTCACGCCGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGaaa                              >  1:936379/1‑148 (MQ=255)
                                                                                                                             agaATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTTCAACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGCTTCATCCACTTCGCCAGCATGCTCg                         >  2:922476/1‑149 (MQ=255)
                                                                                                                             agaATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCg                         >  1:293170/1‑149 (MQ=255)
                                                                                                                              taaTAGCAAAAGGGCAGAGCCAGTGGCCGTGCACTTATCAGTTATCAGGCCTCAGGGTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGc                        <  2:990193/148‑1 (MQ=255)
                                                                                                                               aaTAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGcc                       >  1:303275/1‑149 (MQ=255)
                                                                                                                                 tAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAg                     <  2:109988/149‑1 (MQ=255)
                                                                                                                                   gCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGGGCTTCAGGATTCGGCGCTGGCCGCTTCATCAACTTCGCCAGACTGCTCGCCAGtt                   >  1:123988/1‑149 (MQ=255)
                                                                                                                                     aaaaGGTCAGATCAATTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGTTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACTCTGCGATTCAGGATTCGTCGCTGGCATATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc                 <  2:188097/149‑1 (MQ=255)
                                                                                                                                     aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc                 <  1:407877/149‑1 (MQ=255)
                                                                                                                                     aaaaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTc                 <  1:483373/149‑1 (MQ=255)
                                                                                                                                       aaGGGCTGAGCCAGTGGCCCTGCCATTATCAGTTATCCGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg               <  2:961695/149‑1 (MQ=255)
                                                                                                                                       aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg               <  1:262182/149‑1 (MQ=255)
                                                                                                                                       aaGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg               <  1:802171/149‑1 (MQ=255)
                                                                                                                                        agggcagggcCACTGGCTCTGCCCTTATCAGTTATCAGGCCTCAGGTTGCTGAGCCGGTACCGGGGGTTCCACTACGCCTTCAAGCGGCGCGGAAGGATTCGGCGCTGGCAGATTCATCAACATCGCCCGAATGATCACAAGATTCAgg              >  1:422588/1‑149 (MQ=255)
                                                                                                                                         gggCAGGGCCACTGGCTCTGCCCTGATAAGTTATCAGGCCTAAGGTTCCTGATCCGGTACCGGGGGTACCACAAAGCCTTCACGCGCCGCTTCAGCATTCGGCGCTGGCTGCTTCATCCACTTCGCCCGAAAGCTCGCCAGTTTCCGgc             >  2:127326/1‑149 (MQ=255)
                                                                                                                                         gggCAGAGCCAGTGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcg            >  1:316470/1‑149 (MQ=255)
                                                                                                                                          ggCAGGGCCACTGGCTCTGCCCTTTTCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGgcg            >  1:545123/1‑149 (MQ=255)
                                                                                                                                           gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg               >  2:250850/1‑145 (MQ=255)
                                                                                                                                           gCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAg               <  1:250850/145‑1 (MQ=255)
                                                                                                                                               cgCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCAttt       <  2:1016560/148‑1 (MQ=255)
                                                                                                                                                 ccAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTcc     >  1:475830/1‑149 (MQ=255)
                                                                                                                                                 ccAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGCACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTcc     <  2:590930/149‑1 (MQ=255)
                                                                                                                                                   aGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCcgg   <  2:118017/149‑1 (MQ=255)
                                                                                                                                                    gTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCcgga  >  2:806752/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGCTCAAGGCCGAGATCGATAGTTTTACTGTGCAGGTTTTCCAGATAAGAAAGCCACGAAGCCAGAGGCGACGCGGCTTGAGGAGTGCGTTTGATAATCATGGTATCCGCTGATTCGTTACGGTGAGAATAGCAAAAGGGCAGAGCCAGTGGCCCTGCCCTTATCAGTTATCAGGCCTCAGGTTCCTGATCCGGTACCGGGGGTACCACTACGCCTTCACGCGGCGCTTCAGGATTCGGCGCTGGCAGATTCATCAACTTCGCCAGAATGCTCGCCAGTTTCAGGCGCATTTCCGGA  >  NC_000913/2432842‑2433138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: