Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 5,799 C→A 6.5% E221* (GAA→TAA)  yaaA ← peroxide resistance protein, lowers intracellular iron

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009135,7990CA 6.5% 1871.8 / 10.4 585E221* (GAA→TAA) yaaAperoxide resistance protein, lowers intracellular iron
Reads supporting (aligned to +/- strand):  ref base C (273/250);  new base A (3/59);  total (276/309)
Fisher's exact test for biased strand distribution p-value = 1.78e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 602 total aligned reads displayed.

GCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACG  >  NC_000913/5650‑5948
                                                                                                                                                     |                                                                                                                                                     
gCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTc                                                                                                                                                       >  2:5295980/1‑150 (MQ=255)
gCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTc                                                                                                                                                       <  1:3322295/150‑1 (MQ=255)
  gTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCaa                                                                                                                                                     >  2:1912701/1‑150 (MQ=255)
      aCGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCaataat                                                                                                                                                 >  1:7491117/1‑150 (MQ=255)
        gccgcATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCGATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGa                                                                                                                                               >  2:8260107/1‑150 (MQ=255)
                    aTTGACTGAAAAATTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGTGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCTTTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACtcatca                                                                                                                                   <  2:4558614/150‑1 (MQ=255)
                          tGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACcgc                                                                                                                             >  1:5669250/1‑150 (MQ=255)
                          tGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACcgc                                                                                                                             >  2:1266953/1‑150 (MQ=255)
                            cAAACTTACCGCTGCTCGTAGCGTTTAAACACCAGTTCGCAATTGCTGGAGGAATCTTCATCAAATAAGTAACCTTCGCTATTAAAACCAGCCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGt                                                                                                                           <  2:6639830/150‑1 (MQ=255)
                            cAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGt                                                                                                                           >  1:1398625/1‑150 (MQ=255)
                             aaaCTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTg                                                                                                                          <  2:4674324/150‑1 (MQ=255)
                                 ttAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCttt                                                                                                                      >  2:8684426/1‑150 (MQ=255)
                                  tAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAACGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATATTGAAACGACTCATCAGACCGCGTGCTTTc                                                                                                                     <  2:6105033/150‑1 (MQ=255)
                                    aCGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTCCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCtt                                                                                                                   <  2:169732/150‑1 (MQ=255)
                                      gctgctCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAg                                                                                                                 >  2:503009/1‑150 (MQ=255)
                                       ctgctCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAATAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGc                                                                                                                <  2:5654022/150‑1 (MQ=255)
                                          ctCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTa                                                                                                             >  1:4629316/1‑150 (MQ=255)
                                           tCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAg                                                                                                            >  2:5319485/1‑150 (MQ=255)
                                                gCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCt                                                                                                       >  2:6962618/1‑150 (MQ=255)
                                                  ttttAAACACCTGTCCGCCTTGGCTGGTGTAATCTCCAAAAAATACTTAACCTCCGATATAAAAACCAGTCTGTTGCTCTGGTTTGGTCAGCCTTTTTAAAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCtga                                                                                                     <  1:3611854/149‑1 (MQ=255)
                                                  gTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCtga                                                                                                     >  2:4203856/1‑150 (MQ=255)
                                                  gTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCtga                                                                                                     <  2:5609647/150‑1 (MQ=255)
                                                   tttAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCtgat                                                                                                    >  2:2672862/1‑150 (MQ=255)
                                                   tttAAACACCAGTTCGACATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTTGAAGCtgat                                                                                                    >  1:8504381/1‑150 (MQ=255)
                                                   ttaaaaCCCCCGTTCGCCATTGCTGGAGGAATCTTCAACAAAGAAGTAACCTTCGCTATTAAAACACGTCAGTTGCTCTGGTTTGGTCAGCCGTTTTTAAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCtgat                                                                                                    <  2:2219457/147‑1 (MQ=255)
                                                       aaCACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCCGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATc                                                                                                <  2:1780307/150‑1 (MQ=255)
                                                       aaCACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATc                                                                                                >  1:426478/1‑150 (MQ=255)
                                                       aaCACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATc                                                                                                >  2:40695/1‑150 (MQ=255)
                                                        accccAGTTCGCCATTGCGGGTGTATTCTACATAAAAGAATTAACCTTCGCTTTTAAAACCCGTCAGTTGCTCTGGTTTGGTCAGCCTATTTTCAATATTGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCt                                                                                               <  1:4853316/147‑1 (MQ=255)
                                                        acacCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCt                                                                                               >  1:5415601/1‑150 (MQ=255)
                                                          acCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTa                                                                                             >  1:778939/1‑150 (MQ=255)
                                                                tCGCCTTTGCTGTTTTAATCTTAATAAAATAATTAACATTCTCTATTAAAACCAGTCAGTTGCTCTGTTTTGGTCAGCCTTTTTTAATTATTTAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTg                                                                                       <  1:5971050/150‑1 (MQ=255)
                                                                  gCCTTTGCTGGAGGAATCTTCAACAAAGAAGTAACCTCCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGcc                                                                                     <  1:1015152/150‑1 (MQ=255)
                                                                  gCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGcc                                                                                     >  1:512587/1‑150 (MQ=255)
                                                                      tggCTGGTGTAATCTACATCAAATAAGTAACCTTCGATATTAAAACCATTCCGTTGCTCTGGTTTGGTCAGCCTATTTAAAATAATGACACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGttc                                                                                 <  1:7297890/148‑1 (MQ=255)
                                                                       tGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCTGTTGCTCTGGTTTGGACAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGttct                                                                                <  2:7400780/150‑1 (MQ=255)
                                                                          tGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTtcttct                                                                             <  2:8558829/150‑1 (MQ=255)
                                                                          tGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTtcttct                                                                             >  1:8359939/1‑150 (MQ=255)
                                                                             aggaATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTGGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCAt                                                                          <  2:2889502/150‑1 (MQ=255)
                                                                                aaTCTTCAACAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGACCGCCTATTTTCAATAATGACACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGa                                                                       <  1:2128009/150‑1 (MQ=255)
                                                                                 aTCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCTGTTGCTCTGGTTTGGTCCGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAg                                                                      <  1:2642774/150‑1 (MQ=255)
                                                                                 aTCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAg                                                                      <  2:7584857/150‑1 (MQ=255)
                                                                                  tCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAgg                                                                     >  2:7278342/1‑150 (MQ=255)
                                                                                    ttCAGCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGaa                                                                   <  2:7246773/150‑1 (MQ=255)
                                                                                     tcatcaAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAac                                                                  >  1:7128372/1‑150 (MQ=255)
                                                                                        tcaAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACAcc                                                               <  2:3102883/150‑1 (MQ=255)
                                                                                         caAAGAAGTACCCTTCGCTATTAAAACCATTCCGTTGCTGTGGTTTGGTCAGCCGATTTTAAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCg                                                              <  2:8359939/150‑1 (MQ=255)
                                                                                          aaaGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCgg                                                             >  1:2059588/1‑150 (MQ=255)
                                                                                           attaagTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGTTCAGCCTTTTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGc                                                            <  2:645749/147‑1 (MQ=255)
                                                                                           aagaagTAACCTTCGCTATTAAAACCAGTCTGTTGCTCTGGTTTGGTCAGCCGTTTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGc                                                            <  1:7873761/150‑1 (MQ=255)
                                                                                           aagaagTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTAAATAATGAAAAGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAATCTGATGATCTTAAATTTGACGTTCTTCTCATCGAGGAACACCGGc                                                            >  1:6177899/1‑150 (MQ=255)
                                                                                             gaagTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCtt                                                          >  2:4490067/1‑150 (MQ=255)
                                                                                             gaagTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCtt                                                          <  2:7645855/150‑1 (MQ=255)
                                                                                              atgTAACCTTCGCTATTAAAACCTGTCTGTTGCTGTGGTTTGGTCAGCCTATTTTCAATATTGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTg                                                         <  1:3743584/148‑1 (MQ=255)
                                                                                                ttAACCTTCGATATTAAACCCAGTCAGTTGCTCTGTTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGAt                                                       <  1:6843541/149‑1 (MQ=255)
                                                                                                  aaCCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATaa                                                     <  2:4359964/150‑1 (MQ=255)
                                                                                                      ttCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAAtctc                                                 <  2:7838903/150‑1 (MQ=255)
                                                                                                      ttCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAAtctc                                                 >  1:1096770/1‑150 (MQ=255)
                                                                                                       tCGCTATTAAAACCAGTCTGTTGCTCTGTTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCg                                                <  1:1717320/150‑1 (MQ=255)
                                                                                                       tCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCg                                                <  1:7957157/150‑1 (MQ=255)
                                                                                                        cGCTATTAACACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTACAATAATGACACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCgg                                               <  1:1610624/150‑1 (MQ=255)
                                                                                                        cGCTATTAAAACCAGTCTGTTGCTCTGGTTTGGTCCGCCTTTTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCgg                                               <  1:8212343/150‑1 (MQ=255)
                                                                                                        cGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCgg                                               <  1:4035148/150‑1 (MQ=255)
                                                                                                         gCTATTAAAACCAGTCTGTTGCTCTGGTTTGGTCAGCCTATTTTAAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGc                                              <  1:1510204/150‑1 (MQ=255)
                                                                                                         gCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGc                                              >  2:6876141/1‑150 (MQ=255)
                                                                                                           tATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCAt                                            >  1:127487/1‑150 (MQ=255)
                                                                                                           tATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCAt                                            >  2:8114455/1‑150 (MQ=255)
                                                                                                             ttAAAACCATTCCTTTGATGTGGTTTGTTCCGCAGATTTTCAATTTTGACACGACTCATCAGACCGAGTTCTTTCTTAGCGTAGAATCTGATGATCTTAAATTTGACGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTc                                          <  2:789995/150‑1 (MQ=255)
                                                                                                             ttAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTc                                          >  2:5804891/1‑150 (MQ=255)
                                                                                                               aaaaCCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTATTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCaa                                        >  2:2502148/1‑150 (MQ=255)
                                                                                                                  aCCAGTCCGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGACACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAAttt                                     <  2:6779390/150‑1 (MQ=255)
                                                                                                                  aCCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAAttt                                     <  2:1455493/150‑1 (MQ=255)
                                                                                                                   ccAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATttc                                    >  2:2004130/1‑150 (MQ=255)
                                                                                                                   ccAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATttc                                    >  2:5499392/1‑150 (MQ=255)
                                                                                                                     agtcagtTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATttctt                                  <  2:3406139/150‑1 (MQ=255)
                                                                                                                      gtcagtTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATttcttc                                 <  2:3962136/150‑1 (MQ=255)
                                                                                                                             gCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCa                          <  1:6962618/150‑1 (MQ=255)
                                                                                                                              ctctGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCAc                         >  1:5320106/1‑150 (MQ=255)
                                                                                                                               tctGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCAcc                        >  2:232821/1‑150 (MQ=255)
                                                                                                                               tctGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCAcc                        >  1:6759086/1‑150 (MQ=255)
                                                                                                                                ctGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCg                       >  1:4977296/1‑150 (MQ=255)
                                                                                                                                 tGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGa                      >  1:8706335/1‑150 (MQ=255)
                                                                                                                                 tGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGa                      >  1:5814321/1‑150 (MQ=255)
                                                                                                                                  ggTTGGGTCCGCCGATGTTAAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGAt                     <  1:2499657/150‑1 (MQ=255)
                                                                                                                                   gTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGAtt                    <  1:2954209/150‑1 (MQ=255)
                                                                                                                                     ttGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTa                  >  2:2389779/1‑150 (MQ=255)
                                                                                                                                      tGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTaa                 <  2:2078844/150‑1 (MQ=255)
                                                                                                                                      tGGACAGCCGATTTTCAATATTGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTaa                 <  1:981916/150‑1 (MQ=255)
                                                                                                                                        gTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTaaaa               <  1:5467642/150‑1 (MQ=255)
                                                                                                                                         tCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAAt              >  1:4462835/1‑150 (MQ=255)
                                                                                                                                          cAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATa             <  2:3555258/150‑1 (MQ=255)
                                                                                                                                           aGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATAc            >  2:3733224/1‑150 (MQ=255)
                                                                                                                                            gCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACt           >  1:590222/1‑150 (MQ=255)
                                                                                                                                             ccGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTc          <  1:6465900/150‑1 (MQ=255)
                                                                                                                                             ccGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTc          >  2:1195050/1‑150 (MQ=255)
                                                                                                                                               gATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCAt        <  1:2794686/150‑1 (MQ=255)
                                                                                                                                               gATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCAt        >  1:6934839/1‑150 (MQ=255)
                                                                                                                                                aTTTTCAATACTGACACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATc       <  2:260858/150‑1 (MQ=255)
                                                                                                                                                aTTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATc       >  1:6424395/1‑150 (MQ=255)
                                                                                                                                                   ttCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGa    <  2:7954598/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCGTAGAAGCTGATGATCTTAAATTTGCCGTTCTTCTCATCGAGGAACACCGGCTTGATAATCTCGGCATTCAATTTCTTCGGCTTCACCGATTTAAAATACTCATCTGACG  >  NC_000913/5650‑5948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: