Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,549 G→T 5.3% S63Y (TCC→TAC)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5490GT 5.3% 1133.4 / 5.5 377S63Y (TCC→TAC) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (199/140);  new base T (5/32);  total (204/173)
Fisher's exact test for biased strand distribution p-value = 1.06e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 385 total aligned reads displayed.

AGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAAC  >  NC_000913/238401‑238698
                                                                                                                                                    |                                                                                                                                                     
   tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                                   <  1:1499810/150‑1 (MQ=255)
    aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                                                  >  1:8557504/1‑150 (MQ=255)
    aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                                                  >  1:245906/1‑150 (MQ=255)
      tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                                                <  2:5367760/150‑1 (MQ=255)
       aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                                               <  2:7151521/150‑1 (MQ=255)
        aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                                                              >  1:5298145/1‑150 (MQ=255)
        aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                                                              >  1:729173/1‑150 (MQ=255)
        aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                                                              >  2:3801799/1‑150 (MQ=255)
            tGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaa                                                                                                                                          >  2:1125323/1‑150 (MQ=255)
                aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                                                      <  2:4186032/150‑1 (MQ=255)
                aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                                                      <  2:8600338/150‑1 (MQ=255)
                   aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                                                                   >  1:1730908/1‑150 (MQ=255)
                          cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                                            <  2:7871781/150‑1 (MQ=255)
                          cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                                            >  2:5145167/1‑150 (MQ=255)
                          attttattaAAAATATAATTGTTCGGTGTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTGTTTAATATTTATTTTTTTTTCTTATTAAAAATTAAATTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGaa                                                                                                                            <  1:1965291/147‑1 (MQ=255)
                            aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                                          >  2:689842/1‑150 (MQ=255)
                                 tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                                                     >  2:1760016/1‑150 (MQ=255)
                                 tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGAATACATTCGTTTTATTGATTAACAATGATTTAATTATCGTAATTAAAAAAACAATAAAATATAAAATTGAAAAACGCAAGAAACAGAATAACATAATaaaaaa                                                                                                                     >  1:1876531/1‑147 (MQ=255)
                                   aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                                   >  1:2889199/1‑150 (MQ=255)
                                     aaTATAAGAGTTAGCTGTATTGTTAGGTCTTTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                                 <  2:4990698/150‑1 (MQ=255)
                                        ataAGAGTTAGCTGTATTGTTATGTCTTTGGCCAATTTTACAACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                                              <  1:7981722/150‑1 (MQ=255)
                                         tacgcgTTCGCTTTTTTTTTATGTCTGTGGAGAAATTTAATACCTTCTTTTTTTTGATTAATAATTATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                                             <  2:7319446/145‑1 (MQ=255)
                                         taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                                             <  2:1881080/150‑1 (MQ=255)
                                         taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCaaa                                                                                                             >  1:7813862/1‑149 (MQ=255)
                                              tttAGCTGTATTTTTTTGTCTGTGGCTAAATTGACTACATTCTTTTTTTTGATTAACAATGATTTTATTACCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaa                                                                                                        <  1:6962161/149‑1 (MQ=255)
                                                    tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTATAATTATTTTTTTATAGTAAGTAAAATTAAAAGAAAATTTTAAAAGGAAAAAGACATTAAAACGAATCACAGAATAAAAAATATACAATCaaaaaaaaat                                                                                                  >  1:5550257/1‑146 (MQ=255)
                                                    tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAAATAAATGAATATTTAAAAAGGGAAACCAAATGAAACCCAAGCACAGAATCAAAAAAAAACAATCAAAAAATAtt                                                                                                  >  1:3500800/1‑150 (MQ=255)
                                                     tttttGTTATGTCTGTGTCTAAATTGACTACCTTCGTTTTTTTTATTAATAATGATTTTATTATCGTAATTAAAATTACTTGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                                 <  1:2044488/147‑1 (MQ=255)
                                                     gTATTGTTTTGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAATAATGTTTTTATTATCGTAATTAAATTTACTTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                                 <  1:7215430/150‑1 (MQ=255)
                                                     gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                                 >  2:5142705/1‑150 (MQ=255)
                                                      ttttGTTATGTCTGTGGCTAAATTGACTACATCCGTTTTTTTGAAAAATAATGATTTTATTATCGAAATTAAAATTACATTAATATTTAAATTGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                                                <  1:690674/148‑1 (MQ=255)
                                                      tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACAAGAAACCGAAGCACAGAAACAACATTCTCCAAAAATAAAATATTTc                                                                                                >  2:3696118/1‑150 (MQ=255)
                                                      tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                                                >  2:1377166/1‑150 (MQ=255)
                                                      tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                                                >  2:6254244/1‑150 (MQ=255)
                                                      tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                                                >  2:4340388/1‑150 (MQ=255)
                                                       aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAAACGAAGCACAGAAAAAACAATATCCAAAAAAAAAAAAtttac                                                                                               >  1:294887/1‑148 (MQ=255)
                                                          gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                                            <  2:8296127/150‑1 (MQ=255)
                                                                       cGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTTTTATAGGAAGTAAAATTACATGAATATTTAAAAAGGGAAAACACAAGAAACCGCAGCACAGAAAAAACAATATCCAAACAAAAAAAATTTACGTGGAGCATTttatta                                                                               >  1:1254988/1‑150 (MQ=255)
                                                                       cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAGTttatta                                                                               >  2:8037665/1‑150 (MQ=255)
                                                                       cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                                               >  2:5788499/1‑150 (MQ=255)
                                                                        gAACTTGACTACCTTCGTTTTTTTGATCAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                                              <  2:5942321/150‑1 (MQ=255)
                                                                               aCTACCTGCGTTTTTTTGATTAAGAATGATTTTCTTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAatat                                                                       <  1:4571459/150‑1 (MQ=255)
                                                                                 tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                                                     <  2:3341482/150‑1 (MQ=255)
                                                                                  aCCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                                                    <  1:478431/150‑1 (MQ=255)
                                                                                   ccTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                                   <  2:6015793/150‑1 (MQ=255)
                                                                                   ccTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAATGAAAACGGCATGAAACCGAAGCACAGAATAAACATTATCCAATCATAAAATAATTACGTGGAGGATTTTATTATTGTATATagag                                                                   >  2:4478624/1‑150 (MQ=255)
                                                                                   ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                                   <  2:7933667/150‑1 (MQ=255)
                                                                                         tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                                             >  1:3953945/1‑150 (MQ=255)
                                                                                           tttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACt                                                           >  1:667785/1‑150 (MQ=255)
                                                                                             tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                                         >  2:4056366/1‑150 (MQ=255)
                                                                                              ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGTATTTTATTATTGAATATAGAGGTTTAACTCCg                                                        >  1:2622356/1‑150 (MQ=255)
                                                                                                 aTTAAGAATTATTTTATTATCTTAAGTAAAATTACATTTATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                                     <  1:502632/150‑1 (MQ=255)
                                                                                                  ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                                    >  2:282311/1‑150 (MQ=255)
                                                                                                   tAAGAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                                   <  2:3002550/150‑1 (MQ=255)
                                                                                                      gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                                                <  1:516899/150‑1 (MQ=255)
                                                                                                      gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                                                <  1:5753879/150‑1 (MQ=255)
                                                                                                       aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTGAAAAAc                                               >  1:7881038/1‑150 (MQ=255)
                                                                                                       aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                                               >  1:6513018/1‑150 (MQ=255)
                                                                                                        aTGATTTTTTTATCGTAAGTAAAATTACATGAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                                              <  2:5309803/150‑1 (MQ=255)
                                                                                                          taTTTTATTATCTTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                                            <  1:832920/149‑1 (MQ=255)
                                                                                                          gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                                            >  2:2015434/1‑150 (MQ=255)
                                                                                                          gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                                            >  1:7918068/1‑150 (MQ=255)
                                                                                                          gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                                            >  1:4421755/1‑150 (MQ=255)
                                                                                                           aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                                           >  1:103209/1‑150 (MQ=255)
                                                                                                           aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                                           >  1:2371230/1‑150 (MQ=255)
                                                                                                            ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                                          >  2:2291485/1‑150 (MQ=255)
                                                                                                             tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                                         >  2:6728752/1‑150 (MQ=255)
                                                                                                              ttattaTCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaag                                        <  2:32006/150‑1 (MQ=255)
                                                                                                              ttattaTCCTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaag                                        <  2:5550257/150‑1 (MQ=255)
                                                                                                                attatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGACTATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa                                      >  2:7021766/1‑150 (MQ=255)
                                                                                                                  tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                                    >  1:1105611/1‑150 (MQ=255)
                                                                                                                  tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                                    <  2:2917671/150‑1 (MQ=255)
                                                                                                                    tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa                                  >  2:2967713/1‑150 (MQ=255)
                                                                                                                    tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa                                  >  1:8165750/1‑150 (MQ=255)
                                                                                                                     cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                                 <  1:6391790/150‑1 (MQ=255)
                                                                                                                       taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg                               >  2:2536645/1‑150 (MQ=255)
                                                                                                                        aagtaaAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGc                              <  1:302608/150‑1 (MQ=255)
                                                                                                                         agtaaAATTACACGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa                             >  1:3200802/1‑150 (MQ=255)
                                                                                                                           taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg                           <  2:2705882/150‑1 (MQ=255)
                                                                                                                           taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg                           >  1:6280818/1‑150 (MQ=255)
                                                                                                                             aaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGa                         >  1:1183357/1‑150 (MQ=255)
                                                                                                                             aaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGa                         >  1:2666941/1‑150 (MQ=255)
                                                                                                                                    aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa                  >  1:8239680/1‑150 (MQ=255)
                                                                                                                                    aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa                  >  2:7763798/1‑150 (MQ=255)
                                                                                                                                     tGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataat                 >  2:4808152/1‑150 (MQ=255)
                                                                                                                                      gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata                >  2:8477249/1‑150 (MQ=255)
                                                                                                                                       aaTATATAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAATTAAGTCGAGCATTTTATAATTGAATATAGAGCTTTAAATACGGTAAAAAACAAAGAATCATTGAATGAAGGGAAAAataataa               >  1:6016083/1‑149 (MQ=255)
                                                                                                                                         tatTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATgg             <  1:15454/150‑1 (MQ=255)
                                                                                                                                         tatTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATgg             >  2:6347335/1‑150 (MQ=255)
                                                                                                                                         tatTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATgg             >  1:4130804/1‑150 (MQ=255)
                                                                                                                                         tatTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATgg             <  2:7537628/150‑1 (MQ=255)
                                                                                                                                          atTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGc            >  1:163921/1‑150 (MQ=255)
                                                                                                                                           tttAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGcc           <  2:6450423/150‑1 (MQ=255)
                                                                                                                                            ttAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCa          >  2:1224140/1‑150 (MQ=255)
                                                                                                                                              aaaaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATa        >  2:2443467/1‑150 (MQ=255)
                                                                                                                                               aaaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATaa       <  2:6148877/150‑1 (MQ=255)
                                                                                                                                               aaaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTAATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATaa       >  2:5652940/1‑150 (MQ=255)
                                                                                                                                                aaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATaaa      >  2:2159855/1‑150 (MQ=255)
                                                                                                                                                aaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATaaa      >  2:8411977/1‑150 (MQ=255)
                                                                                                                                                    gAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAAc  <  2:4583985/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAAC  >  NC_000913/238401‑238698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: