Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,596,781 A→T 6.5% K264N (AAA→AAT lgoD → L‑galactonate oxidoreductase; L‑gulonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,596,7810AT 6.5% 1463.7 / 5.3 471K264N (AAA→AATlgoDL‑galactonate oxidoreductase; L‑gulonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (273/131);  new base T (2/43);  total (275/196)
Fisher's exact test for biased strand distribution p-value = 2.52e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

GCCGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTA  >  NC_000913/4596632‑4596930
                                                                                                                                                     |                                                                                                                                                     
 ccGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAg                                                                                                                                                      >  1:3036513/1‑150 (MQ=255)
 ccGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAg                                                                                                                                                      >  2:3121807/1‑150 (MQ=255)
   gAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGt                                                                                                                                                    >  1:5397754/1‑150 (MQ=255)
     aGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGa                                                                                                                                                  >  1:331573/1‑150 (MQ=255)
      gATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAg                                                                                                                                                 >  1:1129564/1‑150 (MQ=255)
      gATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAg                                                                                                                                                 <  2:7718664/150‑1 (MQ=255)
       aTTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGt                                                                                                                                                >  1:1553872/1‑150 (MQ=255)
        ttttGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGtt                                                                                                                                               >  1:4000864/1‑150 (MQ=255)
           tGACGCGCCGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCa                                                                                                                                            <  2:1675929/150‑1 (MQ=255)
           tGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCa                                                                                                                                            <  2:514978/150‑1 (MQ=255)
           tGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCa                                                                                                                                            <  1:7755984/150‑1 (MQ=255)
            gACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAg                                                                                                                                           >  1:3586287/1‑150 (MQ=255)
              cgcgCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGtt                                                                                                                                         >  2:3503242/1‑150 (MQ=255)
               gcgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAAGTTGCGGTGAATAACACCGTGAATTTGATTCGTCACGGGGGCTCGGTGGTATTTGTGGGCGTGTTTATAGGTGAGTTGCTGttg                                                                                                                                        >  2:4774517/1‑149 (MQ=255)
               gcgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtc                                                                                                                                        >  2:5804305/1‑150 (MQ=255)
               gcgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtc                                                                                                                                        >  2:276539/1‑150 (MQ=255)
               gcgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtc                                                                                                                                        >  2:5416863/1‑150 (MQ=255)
               gcgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtc                                                                                                                                        >  1:6213409/1‑150 (MQ=255)
                cgcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtct                                                                                                                                       <  2:229791/150‑1 (MQ=255)
                 gcAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtctc                                                                                                                                      <  1:4724137/150‑1 (MQ=255)
                    gCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGa                                                                                                                                   >  2:4417224/1‑150 (MQ=255)
                    gCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGa                                                                                                                                   >  1:7339131/1‑150 (MQ=255)
                    gCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGa                                                                                                                                   >  2:8703869/1‑150 (MQ=255)
                         gggCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCgg                                                                                                                              >  2:5568810/1‑150 (MQ=255)
                          ggCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGa                                                                                                                             >  1:8112663/1‑150 (MQ=255)
                            cgcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAt                                                                                                                           >  2:4062219/1‑150 (MQ=255)
                             gcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAtt                                                                                                                          >  2:2955853/1‑150 (MQ=255)
                             gcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAtt                                                                                                                          >  2:1407104/1‑150 (MQ=255)
                             gcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAtt                                                                                                                          <  2:1553872/150‑1 (MQ=255)
                             gcAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAAtt                                                                                                                          >  2:3255971/1‑150 (MQ=255)
                               aGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTcc                                                                                                                        >  1:57593/1‑150 (MQ=255)
                                gTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCa                                                                                                                       >  1:4200311/1‑150 (MQ=255)
                                gTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCGGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCa                                                                                                                       >  2:5057189/1‑150 (MQ=255)
                                  ttGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATa                                                                                                                     <  1:988578/150‑1 (MQ=255)
                                  ttGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATa                                                                                                                     <  2:3607188/150‑1 (MQ=255)
                                  ttGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATa                                                                                                                     <  1:7721187/150‑1 (MQ=255)
                                     gtggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaaa                                                                                                                  <  2:2538469/150‑1 (MQ=255)
                                     gtggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaaa                                                                                                                  >  1:2588337/1‑150 (MQ=255)
                                       ggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaaaaa                                                                                                                <  2:5365621/150‑1 (MQ=255)
                                       ggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaaaaa                                                                                                                <  1:8060861/150‑1 (MQ=255)
                                       ggtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATaaaaaa                                                                                                                <  1:6356003/150‑1 (MQ=255)
                                        gtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAg                                                                                                               >  2:6135167/1‑150 (MQ=255)
                                        gtTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAg                                                                                                               >  1:6502210/1‑150 (MQ=255)
                                         ttCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGa                                                                                                              >  1:4600732/1‑150 (MQ=255)
                                            gCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAac                                                                                                           >  2:5730360/1‑150 (MQ=255)
                                              tGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAacga                                                                                                         <  2:7454229/150‑1 (MQ=255)
                                                    aGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACgatga                                                                                                   >  2:8391790/1‑150 (MQ=255)
                                                          tGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCa                                                                                             >  1:5495145/1‑150 (MQ=255)
                                                            aTCGACGCGACAGGTAATCAACATGCTATGAATAACACCGTGAATTTTTTTCGTCAGGGGGGCACTGTGTTTTTTGTGGGCCTGTTTAAAGGTTTGTTGCTGTTCTCGGTTCCGTAATTCATTAAAAAATAAACGACGATGATGGGCAGc                                                                                           <  2:3671958/150‑1 (MQ=255)
                                                            aTCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTtctc                                                                                                                                      >  1:4928781/1‑107 (MQ=255)
                                                             tCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGcc                                                                                          >  2:4622898/1‑150 (MQ=255)
                                                               gACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGAGGATGGGCAGCCGc                                                                                        >  1:1278699/1‑150 (MQ=255)
                                                                aCGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCa                                                                                       >  1:1440224/1‑150 (MQ=255)
                                                                 cgcgACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCaa                                                                                      >  1:6919296/1‑150 (MQ=255)
                                                                    gactgttAATCAACAGGCGCTGAATAACACCGTTATTTTTATTCGGCAGGGCGGCCCGGTGGTATTTGTGGGCCTGTTTAACGGTGAGTTGCAGTTCTCCTATCGGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgc                                                                                   <  2:3429910/144‑1 (MQ=255)
                                                                    gACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgc                                                                                   >  2:7413883/1‑150 (MQ=255)
                                                                    gACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgc                                                                                   >  1:8227916/1‑150 (MQ=255)
                                                                     aCAGGTAATCAACATGCGATGAATAACACCGTGAATTTGTTTCGTCACGGCGGCAGGGTGGTATTTGTCGGCCTTTTTAAAGGTGAGTTGCATTTCTCCTATCCGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgcc                                                                                  <  2:4901690/150‑1 (MQ=255)
                                                                       aGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccac                                                                                >  1:5222556/1‑150 (MQ=255)
                                                                        ggTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGAAAATGACGGCCGGTTTAAAGGTGAGTTGCAGTACACCGATCCGGAATTCAATAAAAAAGAAACGACGATGATGGGCAGCCGAAactcaacg                                                                               >  2:8627182/1‑150 (MQ=255)
                                                                         gTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccacgc                                                                              >  2:2879118/1‑150 (MQ=255)
                                                                         gTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccacgc                                                                              >  1:8548493/1‑150 (MQ=255)
                                                                          tAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAacgccacgcc                                                                             >  1:4424270/1‑150 (MQ=255)
                                                                               aaCATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGa                                                                                                                                                  <  1:6797999/76‑1 (MQ=255)
                                                                                 cATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGAt                                                                      >  1:1939844/1‑150 (MQ=255)
                                                                                  aTGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGTTATTTGTCGGCCTTTTTAATGGTGTGTTGCTGTTCTCCTATCCGTAATTCCATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGAtt                                                                     <  2:5971456/150‑1 (MQ=255)
                                                                                  aTGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGAtt                                                                     >  1:7135403/1‑150 (MQ=255)
                                                                                     cGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTg                                                                  >  1:2278922/1‑150 (MQ=255)
                                                                                        tGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTa                                                               >  2:377259/1‑150 (MQ=255)
                                                                                         gAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTaa                                                              >  2:682047/1‑150 (MQ=255)
                                                                                            tAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgt                                                           >  1:1007132/1‑150 (MQ=255)
                                                                                              acacCTTGATTTTGTTTCGTCACGGCGGCACGGTGGTATTTGTCGCCCTGTTTAATGGTGAGTTGCATTTCTCCGATCCGTAATTACATAAAAAACAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcg                                                         <  2:7628753/150‑1 (MQ=255)
                                                                                               cacCGTGAATTTTATTCGTCACGGCGGCAGGGTGGTTTTTGTCGGCCTGTTTAAAGGTGAGTTGAAGTTCTCCGATCCGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcgg                                                        <  2:7919727/150‑1 (MQ=255)
                                                                                                   gTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggtcag                                                    >  1:5332721/1‑148 (MQ=255)
                                                                                                    tGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGAc                                                   >  1:3872573/1‑150 (MQ=255)
                                                                                                        tttGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGAt                                               >  1:665077/1‑150 (MQ=255)
                                                                                                        tttGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGAt                                               >  1:1748180/1‑150 (MQ=255)
                                                                                                            tttCGTCCGGGCGGCCGGGTGTTATTTGTCGGCATTTTTATATGTTTGTGGCCTTGCTCCTATCTGGAATCCCATAAAAAACAATCGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCg                                           <  1:2580655/149‑1 (MQ=255)
                                                                                                            tgtggTCCCGGGGGCTCGTTGTTTTTGGTCGGCTTTTTTAACGGTTTGTTGCTGTTCCCCGACCCGTAATTCCAAAAAAAACACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCg                                           <  2:8033396/146‑1 (MQ=255)
                                                                                                                      ggggcAGGGTGTTATTTGTGGGCCTTTTTAATGGTGTGTTGCAGTTCCCCGATCCGGATTTCAATAAAAAACAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGaaaaa                                 <  2:1790184/148‑1 (MQ=255)
                                                                                                                       cggcACGGTGGTTTTTGTCGGCCTTTTTAATGGTGAGTTGCTGTTCTCCGATCGGTAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAAt                                <  2:6212664/150‑1 (MQ=255)
                                                                                                                        ggcACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATc                               >  1:8360648/1‑150 (MQ=255)
                                                                                                                          ccgggttgtATTTGTCGGCCTGTTTAATGGTGAGTTGCAGTTCTCCTATCGGTAATTCCAAAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCAc                             <  1:601723/147‑1 (MQ=255)
                                                                                                                           aCGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCAAAAAAAAACAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCAct                            <  2:7201845/150‑1 (MQ=255)
                                                                                                                             ggtggtATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgc                          >  2:8581413/1‑150 (MQ=255)
                                                                                                                             ggtggtATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCCCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgc                          >  1:2517679/1‑150 (MQ=255)
                                                                                                                              gtggtATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgct                         >  2:6367085/1‑150 (MQ=255)
                                                                                                                                ggtTTTTGTCGGCCTGTTTATCGGTGTGGGGCAGTTCTCCGATCCGGAATTCCATAAAAAAGACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGa                       <  2:7067582/150‑1 (MQ=255)
                                                                                                                                ggtATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGa                       >  2:1157913/1‑150 (MQ=255)
                                                                                                                                 gtATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGAc                      >  2:202420/1‑150 (MQ=255)
                                                                                                                                    tttGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatg                   >  1:7926330/1‑150 (MQ=255)
                                                                                                                                     ttGTGGGCCTTTTTAATGGTGTGTTGCAGTTCTCCGATCCTTAATTCCAAAAAAAACAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatga                  <  1:869234/150‑1 (MQ=255)
                                                                                                                                     ttGTCGGCTTTTTTAATGGTGTGTTTCAGTTCGCCGCTCCGTAATACAAAAAAAAACACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatga                  <  1:2965677/150‑1 (MQ=255)
                                                                                                                                     ttGCCGGCCTTTTTAATGGTTTGTTGCAGTTCTCCGATCCGTAATTCAATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatga                  <  1:702061/150‑1 (MQ=255)
                                                                                                                                      tGTCGGCCTGTTTAATGGTGAGTTGCAGTTCTCCGATCCGTAATTCCATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgat                 <  2:7264261/150‑1 (MQ=255)
                                                                                                                                      tGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgat                 >  2:5676154/1‑150 (MQ=255)
                                                                                                                                       gTCGGCCTTTTTAAAGGTGAGTTGCAGTTCTCCTATCCGGAATCCCAAAAAAAATAAACGACGATGATGGGCAGCCGCAACACCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACatgatg                <  2:5495145/150‑1 (MQ=255)
                                                                                                                                                tttAATGGTGTGTTGATGTTCTCGGCGCCGTATTTCAATAAAAAATAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt       <  2:3892429/150‑1 (MQ=255)
                                                                                                                                                 ttAAAGGTGCGGTGCTGTTCTCCGATCCGTAATTCAATAAAAAACACACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATc      <  1:3933104/150‑1 (MQ=255)
                                                                                                                                                   aaaGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGc    >  2:6695252/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGAAGATTTTGACGCGCAGCTACGGGCGCAGTTTGGTGGTTCGCTGGCGCAGAAAGTGATCGACGCGACAGGTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTA  >  NC_000913/4596632‑4596930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: