Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,005 A→C 5.1% intergenic (‑124/+64) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0050AC 5.1% 899.3 / 6.8 302intergenic (‑124/+64)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (240/37);  new base C (3/20);  total (245/57)
Fisher's exact test for biased strand distribution p-value = 1.91e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  NC_000913/379856‑380154
                                                                                                                                                     |                                                                                                                                                      
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                       <  2:3826622/150‑1 (MQ=255)
  cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAaga                                                                                                                                                      <  2:8301171/150‑1 (MQ=255)
  cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAaga                                                                                                                                                      <  1:6868391/150‑1 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                     >  2:2263789/1‑150 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                     <  2:7068741/150‑1 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                     >  2:4399271/1‑150 (MQ=255)
   ttctcgtcCGGTGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATGCTATAGGTCAATGCATACCCCCCTATAGTATCTAACACGGGAGAATAAAATTTATCCGGGGAATGTGGTCGGAGAACAAagag                                                                                                                                                     >  2:6642846/1‑150 (MQ=255)
    tctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAgg                                                                                                                                                    >  2:4507921/1‑150 (MQ=255)
      tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                  >  1:7912062/1‑150 (MQ=255)
        ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGTATGTGGTCGGAATACAAAGAGGACAg                                                                                                                                               >  2:5657207/1‑150 (MQ=255)
        ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAACCAAAGAGGAAAg                                                                                                                                               >  2:1597194/1‑150 (MQ=255)
        ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                                                                                                                               >  2:678260/1‑150 (MQ=255)
          ccGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                                                                             <  1:4488866/150‑1 (MQ=255)
          ccGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                                                                             <  1:7303673/150‑1 (MQ=255)
             gAGTACTGGGCATTTCGCACCTAATCATCTGCATGCAATATACTATAGGGGGTTATTCTATATGTCAATGCATACCCCCATATAGTATATAACACTGGAGAATAAAATTTATCCGGTGATTGTGGTCGTAAAACAAAGAGGAAAgggggg                                                                                                                                          <  1:2564291/150‑1 (MQ=255)
               gTACTGGGCATTTCGCACCTCTTCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                                                                                                                        <  2:5569144/150‑1 (MQ=255)
                 aCTGGGCTTTTCGCACCTCATCATCTGCATGCAATATACTATTGGGGGTTATTATATATGTCAATGCATACCCCCCTATAGTATATAACACTGTATAATAAAATTTATCCGTTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggg                                                                                                                                      <  2:6076457/150‑1 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCt                                                                                                                                    >  2:3206958/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAaga                                                                                                                                                      <  2:7935769/131‑1 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTaa                                                                                                                                  >  2:3048207/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTaa                                                                                                                                  >  2:2525141/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTaa                                                                                                                                  >  1:1209575/1‑150 (MQ=255)
                      gCATTTCGCACCTATTCATCTGCATTAAATATACTATAGGGGGTTATTCTATATTTCTATGCATACCCCCCTATTGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAAt                                                                                                                                 <  2:1829033/150‑1 (MQ=255)
                       cATTTCTAAACTCATCTTATGAATGCTATATACTATAGTGTGTTATTCTTTATTTCAATGCATACCCCCCTATATTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACCGCGGAAAGGGGGGGGGGCTAATc                                                                                                                                <  2:6093854/150‑1 (MQ=255)
                        aTTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCg                                                                                                                               >  1:2871012/1‑150 (MQ=255)
                           ttgaaCCTCATCTTTTGATTGCATTATACTTTAGTGGTTTATTCTATATTTCAATTCATACCCCCCTTTTGTATATAACCCTTGCTAATAAAATTTAGCCGGTGAATGTGGTCGGAAAACAAAGCGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                            <  2:2789198/146‑1 (MQ=255)
                           tCGCACCTCATCATCTGCATGCAATATCCGATAGGGGGGTATTCTATATGTCAAGGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                            <  2:4752613/150‑1 (MQ=255)
                             gCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAACGAGGAAAGGGGGGGGGGCGAATCGGCAgg                                                                                                                          >  2:7692792/1‑150 (MQ=255)
                             gCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAgg                                                                                                                          >  1:4134888/1‑150 (MQ=255)
                             gCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAgg                                                                                                                          >  1:3308171/1‑150 (MQ=255)
                                ccTCATCATCTGCACGCAATATCCCATCGGGGGTTTTTCTACATGTCAACGCATCCCCCCCTATAGTATATAACACGGCAGATAAAAATTAAACCGGTGAACGTGGTCGCAAAACAACGACCAAAGGGGGGGGGGCTAATCGGCAGGGaa                                                                                                                       <  2:4545517/150‑1 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAggagatc                                                                                                                     >  1:172526/1‑145 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTAAAGGGGGTTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                     >  2:7222097/1‑150 (MQ=255)
                                     tcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                                                                   >  1:3760246/1‑150 (MQ=255)
                                       atcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                                                                >  1:132300/1‑150 (MQ=255)
                                         cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                                                              >  1:2497148/1‑150 (MQ=255)
                                          tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACGCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGTAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                                                                             >  1:5535854/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAGAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGACGGCCGCCCCGGATa                                                                                                         >  1:6672009/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAtc                                                                                                         >  2:4681436/1‑149 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                         >  1:1413331/1‑150 (MQ=255)
                                               gcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                                                                        >  2:3489651/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTCTAGTATATAACACTGGAGACTCAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAACGGGGGGGGGGCTCATCGGCGGGGAAGGCCGCCCCggcgcgcg                                                                                                      >  1:8199150/1‑144 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                      >  1:2619443/1‑150 (MQ=255)
                                                  atatACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                                                     >  1:6751502/1‑150 (MQ=255)
                                                      aCTATGGGGGGGTATTCTATATGTCAATGCATCCCCCCCTATAGTATATACCACTGGAGAATAAAATGTATCCGGTGAATGTGGGCGGACACCAAAGCGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                                                 <  2:6873291/150‑1 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGACAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                                                                >  2:2461370/1‑150 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATCGCGGGCGGCa                                                                                               >  1:7513866/1‑150 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                               >  1:3942668/1‑150 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                               >  1:5075224/1‑150 (MQ=255)
                                                             gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                          >  1:6133160/1‑150 (MQ=255)
                                                               ggggATACTAAATGTCACTGCAAACCCCCCCCTAGTATATAACCCGGGAGAACACCATCTCCCCGGTGAACGTGCCCGGAAAACACAGCGCAACGGGGGGGGGGCTACTCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                                                                        <  2:8089719/150‑1 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAGGGGGGGGGGCATATAGGCAAGGAAGGCCCCCCCCGATAGCGGGCCGCAGAAggcaac                                                                                      >  2:4306012/1‑146 (MQ=255)
                                                                  tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                                                     >  1:6604022/1‑150 (MQ=255)
                                                                     tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                                                 >  2:1340366/1‑150 (MQ=255)
                                                                           tgacAATGACTACCCCCCTATAGTATATAACACTGGAGAATACCATGGCGCCGGTGAACGTGGCCGGAAAACACCGCGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                            <  2:4926346/147‑1 (MQ=255)
                                                                              cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                          >  1:2175342/1‑150 (MQ=255)
                                                                              cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                                                                         >  1:8084187/1‑150 (MQ=255)
                                                                              cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                                                                         >  1:3141730/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                        >  1:8473067/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                        >  2:5327470/1‑150 (MQ=255)
                                                                                aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                       >  1:6455588/1‑150 (MQ=255)
                                                                                 tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                                                                      >  2:8645172/1‑150 (MQ=255)
                                                                                  gCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                                     >  2:5127585/1‑150 (MQ=255)
                                                                                     tACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                                                                  >  2:109564/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                               >  2:1454027/1‑150 (MQ=255)
                                                                                        cccccTAGACTATACAACCCCGCAGAACACCATTCACCCGGTGAATGTGGCCGCACAACCCCGCGGACAGGGGGGGGGGCTACTCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                               <  2:2202142/150‑1 (MQ=255)
                                                                                            cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                                                           >  1:1982066/1‑150 (MQ=255)
                                                                                             tctcgTATATAACACTGGAGATAAAAATTGCGCCGGGGATTGGGGCCGCAAAACCCCGCGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaac                                                          <  2:3141730/146‑2 (MQ=255)
                                                                                               taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt                                                        >  1:6169214/1‑150 (MQ=255)
                                                                                                     ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCCGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                  >  2:5754451/1‑150 (MQ=255)
                                                                                                          acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGACTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTa                                             <  2:7485536/150‑1 (MQ=255)
                                                                                                           cTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATCGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCACGTTGCAGACCGTTaa                                            >  1:3264186/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                           >  1:5998573/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCCGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                    >  1:4328596/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                    >  1:1541389/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                    >  1:355242/1‑150 (MQ=255)
                                                                                                                    aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg                                   >  1:105510/1‑150 (MQ=255)
                                                                                                                       aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg                                >  1:3422906/1‑150 (MQ=255)
                                                                                                                        tttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgg                               >  2:3461102/1‑150 (MQ=255)
                                                                                                                        tttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgg                               >  2:137090/1‑150 (MQ=255)
                                                                                                                            tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg                           >  2:1397920/1‑150 (MQ=255)
                                                                                                                             ccGGTGACTGTGGCCGGCCCACACCGCGGACAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt                          <  2:6370646/150‑1 (MQ=255)
                                                                                                                             ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTGGGGgtgt                          >  2:2666317/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                        >  1:3863604/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                        >  2:2991996/1‑150 (MQ=255)
                                                                                                                                 tGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa                      >  1:7110088/1‑150 (MQ=255)
                                                                                                                                      gtgGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcaca                 >  1:8318995/1‑150 (MQ=255)
                                                                                                                                         gTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACtt              >  1:799525/1‑150 (MQ=255)
                                                                                                                                         gTCGGAAAACAAAGAGGAAAGGGGGGGGGGCAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAACAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCacacat              >  2:6690478/1‑148 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATAAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACgtt             >  2:2123174/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc           >  1:4749797/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc           >  2:5727194/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc           >  2:4615411/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGGTAAAATCATCGGGTGGGGTGTCGTACCACACTTTAc           >  1:1090465/1‑150 (MQ=255)
                                                                                                                                               aaaCAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTg        >  1:3595526/1‑150 (MQ=255)
                                                                                                                                                accAAAGCCGAACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGc       <  2:3303977/148‑1 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCg     >  1:5770735/1‑150 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGt    >  1:1688440/1‑150 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGt    >  1:6260925/1‑150 (MQ=255)
                                                                                                                                                     agagGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCa  >  1:8715171/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA  >  NC_000913/379856‑380154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: