Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 580,727 G→T 7.9% intergenic (‑282/‑107) ylcI ← / → nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,7270GT 7.9% 1314.8 / 27.5 419intergenic (‑282/‑107)ylcI/nohDDUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
Reads supporting (aligned to +/- strand):  ref base G (228/145);  new base T (4/41);  total (233/186)
Fisher's exact test for biased strand distribution p-value = 5.92e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

CGTTGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGA  >  NC_000913/580578‑580876
                                                                                                                                                     |                                                                                                                                                     
cGTTGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATg                                                                                                                                                       >  1:6441462/1‑150 (MQ=255)
  ttGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGta                                                                                                                                                     >  2:141574/1‑150 (MQ=255)
       aCATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtatttat                                                                                                                                                <  2:4983770/150‑1 (MQ=255)
       aCATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtatttat                                                                                                                                                <  2:2608357/150‑1 (MQ=255)
         aTGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTGTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTatttattt                                                                                                                                              >  1:2323798/1‑150 (MQ=255)
          tGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTAtttattta                                                                                                                                             >  1:620954/1‑150 (MQ=255)
           gTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTaa                                                                                                                                            <  2:3181510/150‑1 (MQ=255)
            tAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTaaa                                                                                                                                           <  1:4207524/150‑1 (MQ=255)
             aGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTaaaa                                                                                                                                          <  1:3056015/150‑1 (MQ=255)
              gatgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAAt                                                                                                                                         >  1:6596671/1‑150 (MQ=255)
              gatgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAAt                                                                                                                                         >  1:3432092/1‑150 (MQ=255)
                tgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAc                                                                                                                                       <  2:7501415/150‑1 (MQ=255)
                 gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      >  1:1666292/1‑150 (MQ=255)
                 gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      >  2:5376635/1‑150 (MQ=255)
                 gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      <  2:5766068/150‑1 (MQ=255)
                  atAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccc                                                                                                                                     <  2:8045758/150‑1 (MQ=255)
                  atAATTATTATCATTTTGTGGGTCATTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCAGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcac                                                                                                                                     >  1:2854913/1‑148 (MQ=255)
                    aaTTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccccc                                                                                                                                   >  1:778919/1‑150 (MQ=255)
                    aaTTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccccc                                                                                                                                   >  1:426336/1‑150 (MQ=255)
                    aaTTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccccc                                                                                                                                   >  1:1734158/1‑150 (MQ=255)
                     attattATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCt                                                                                                                                  <  2:7861646/150‑1 (MQ=255)
                     attattATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCt                                                                                                                                  <  1:198240/150‑1 (MQ=255)
                     attattATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCt                                                                                                                                  >  2:2801471/1‑150 (MQ=255)
                      ttattaTCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTg                                                                                                                                 >  1:5125331/1‑150 (MQ=255)
                          tatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaat                                                                                                                             >  2:8578218/1‑149 (MQ=255)
                          tatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaaa                                                                                                                             <  2:5441098/150‑1 (MQ=255)
                          tatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaaa                                                                                                                             >  1:6802911/1‑150 (MQ=255)
                          tatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaaa                                                                                                                             >  2:6817845/1‑150 (MQ=255)
                            tCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaaga                                                                                                                           >  2:5642221/1‑150 (MQ=255)
                             cATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTTGGGGGGGGCGACCTCGCGGGTTTTCGCTTTTTATGAATATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaa                                                                                                                          <  2:6132222/150‑1 (MQ=14)
                             cATTTTGTGGGTCCTGTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTAGTAAACTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAacagaa                                                                                                                          <  2:7126954/150‑1 (MQ=14)
                              actttacgggTCCTTTCCGGTGATCCGACAGGTTGCGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  2:5826403/143‑1 (MQ=2)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:5853621/150‑1 (MQ=255)
                              aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTGTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         >  2:2102423/1‑150 (MQ=255)
                               ttttGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaag                                                                                                                        >  2:395153/1‑150 (MQ=255)
                                   gtgGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGaaa                                                                                                                    <  2:7380317/150‑1 (MQ=40)
                                        tCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACa                                                                                                               >  1:8497464/1‑150 (MQ=39)
                                        tCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACa                                                                                                               >  2:1269708/1‑150 (MQ=39)
                                           tttCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGt                                                                                                            >  2:4825419/1‑150 (MQ=39)
                                           tttCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGt                                                                                                            >  1:4807404/1‑150 (MQ=39)
                                              ccGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCt                                                                                                         >  2:7561191/1‑150 (MQ=39)
                                                    aTCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGc                                                                                                   >  2:4515737/1‑150 (MQ=38)
                                                     tCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACTACAGGTGCTGAAAGCg                                                                                                  >  2:3739816/1‑150 (MQ=255)
                                                     tCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAACCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCg                                                                                                  >  1:7446791/1‑150 (MQ=255)
                                                       cGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAg                                                                                                >  1:4564577/1‑150 (MQ=39)
                                                        gACAGGTTACGGGGCGGCGACCTCGCGGGTGTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGGCGGAACTTAATGTATTGATTTAAAATACCCCCTGAAAAGAACGGCCACGACAGGTGCGGAAAGCGAGc                                                                                               >  2:6403074/1‑150 (MQ=14)
                                                           aGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCttt                                                                                            >  1:8131535/1‑150 (MQ=39)
                                                           aGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTATTCTTCGTCGTAACTTAATGTATTTATTTTAAATACCCCCTGAAAATAAAGGAAAAGACAGTTGCTGTAATCGAGCttt                                                                                            >  1:4115310/1‑150 (MQ=11)
                                                             tttAGGGGGCGGCGACCTCGCGGTTTTTCGATATTTATTAAAATTTTCCGGTTTAAGGTTTTTCCTTTCTTCTTCGTCTTAACTTATTGTTTTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCttttt                                                                                          <  2:2516590/149‑1 (MQ=11)
                                                             gTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCttttt                                                                                          >  1:7677039/1‑150 (MQ=40)
                                                             gTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCttttt                                                                                          <  2:2110405/150‑1 (MQ=40)
                                                              ttACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTg                                                                                         >  2:5532997/1‑150 (MQ=40)
                                                              ttACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTg                                                                                         >  1:2681116/1‑150 (MQ=40)
                                                                agggggCGGCGACCTCGCGTGTTTTCTCTATTTATTAAAATTTTCCTTTTTTATGTGTTTCCGTTATTATTCTTGTTAACTTAATGTATTTATTTAAAATACCCCCTTAAAATAAATGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGc                                                                                       <  2:7774346/148‑1 (MQ=255)
                                                                       ggcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATCCCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTC                                                                                <  2:1035509‑M1/150‑1 (MQ=255)
                                                                         cgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGT                                                                              >  2:2942344‑M1/1‑150 (MQ=255)
                                                                         cgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTACGGTTTAAGGTGTTTACGTTCTTCTTAGTCGTAACTTAATGTATTTATTTAAAATAACCCCAGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTTGCCACTGTCGT                                                                              >  1:2252983‑M1/1‑150 (MQ=255)
                                                                          gACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTT                                                                             >  2:4358317‑M1/1‑150 (MQ=255)
                                                                          gACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTT                                                                             >  2:4102644‑M1/1‑150 (MQ=255)
                                                                          gACCTCGCGGGTTTTCGCTATTTATGAAAATTTTACGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAATAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTT                                                                             >  1:1007121‑M1/1‑150 (MQ=255)
                                                                             cgcgcgggTTTTCTCTATTTATTAAAATTTTCGGTTTTATGTTTTTTCCGTTCTTCTTCGTCTTAAATTATTTTATTTATTTAAAATACCCCCTCAAAAGAACGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCC                                                                          <  1:365395‑M1/148‑1 (MQ=255)
                                                                              tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATTTATTTATTTAAAATAACCCCTGAAAAGAAATGAAACGACAGGTGATGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCT                                                                         >  1:1452999‑M1/1‑150 (MQ=255)
                                                                                ggggtttttCTTTATTTTTCAAAATTTTCCGGTTTTTGTTGGTCCCGTTCTTCTTCGTCTAAACTTAATTTATTAATTAAAAAAACCCCCATAAACCAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCttt                                                                       <  1:8689885/145‑1 (MQ=9)
                                                                                gcgGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTT                                                                       >  2:2890536‑M1/1‑150 (MQ=255)
                                                                                gcgGGTTTTCGAAATTTATCAAAATTTTCCTGTTTAAGTTGTTACCTTTCTTATTCGTCCTAAATTAATGAATTTATTTAAAATACCCCCTCAAAAGAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTT                                                                       <  2:6289335‑M1/150‑1 (MQ=255)
                                                                                  gggTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCATTTCT                                                                     >  1:57362‑M1/1‑150 (MQ=255)
                                                                                      tttCTCTATTTTTTAAAATTTTCCGGTTTATGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTTTTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGt                                                                 <  2:8704352/150‑1 (MQ=11)
                                                                                      tttCGCTATTTATGAAAATTTGCCGTTTTTCGGTTTTCCCGTTATCCTGCGTCGTAACTTATTGTATTTATTTAAAATACCCCCTCAAAACAATGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGt                                                                 <  2:8124216/150‑1 (MQ=14)
                                                                                          gCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGttttt                                                             >  1:7160283/1‑150 (MQ=255)
                                                                                           cTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTg                                                            >  2:3679287/1‑150 (MQ=255)
                                                                                            tATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTGCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGt                                                           <  2:1891253/150‑1 (MQ=255)
                                                                                             aTTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTc                                                          >  2:333483/1‑150 (MQ=255)
                                                                                                tATTAAAATTTACCGGTTTATGTTGTTTCCGTTCTTCTTCGTCGTAACTTATTGTATTTATTTAAAATACCCCCTGAACAGAAAGTAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGt                                                       <  2:4617514/150‑1 (MQ=14)
                                                                                                     aaaTTTTCGGTTTTATGGTGTTACCTTTCTTCTTCGTCGTAACTTATTGTATTTTTTTAAAACACCCCCGCAAAAGAACGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAAt                                                  <  2:2323798/150‑1 (MQ=14)
                                                                                                      aaTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAAtg                                                 >  1:8282702/1‑150 (MQ=255)
                                                                                                        ttttCGGTTTTTAGGTTTTTCCTTTCTTCTTGTTCTTAAATTATTTTATTTATTAAAAATACCCCATTAAAACAAAGCACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAAtgtg                                               <  1:4004542/150‑1 (MQ=14)
                                                                                                        ttttCCGGTTTAAGGTTTTTCCTTTCTTATTCGTCTTAACTTAATGTTTTTTTTTAAAATACCCCCTTAAATGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAAtgtg                                               <  2:8421045/150‑1 (MQ=11)
                                                                                                              ggTTTATGGTTTTTCGGTTTTTTTTCTTCTTAAATTATTTTATTTATTTAAAAAACCCCCTGAAAACAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATgg                                         <  1:4408837/150‑1 (MQ=14)
                                                                                                               gTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTACTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGa                                        >  1:2498113/1‑150 (MQ=255)
                                                                                                                tttAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                       <  2:3519972/150‑1 (MQ=255)
                                                                                                                      gtgtTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGaaa                                                                                                     >  1:7915193/1‑82 (MQ=38)
                                                                                                                      gtgtTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTcaac                                 >  2:1623964/1‑150 (MQ=255)
                                                                                                                          ttCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGTATGTGCAATGGAAGTCAACaaaa                             >  2:6689827/1‑150 (MQ=255)
                                                                                                                          ttCCGTTCTTCTTCGTCGTAAATTAATTTATTTATTTAAAATACCCCCTGAAAATAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaa                             <  2:1090988/150‑1 (MQ=255)
                                                                                                                           tCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaaa                            >  2:1728252/1‑150 (MQ=255)
                                                                                                                           tCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaaa                            <  2:6526704/150‑1 (MQ=255)
                                                                                                                           tCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAACCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTACTTTATCTGTTTTTGTCAGTGGCATGTGCCATTGTAGTCAACaaaaa                            >  1:131473/1‑150 (MQ=14)
                                                                                                                                     ttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCATTCTCTGTTTTTGTCCGTGGAATGTGCACTGGAAGTCAACAACAAGCAgctggcg                  >  1:3787422/1‑149 (MQ=255)
                                                                                                                                      tcgtcgTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggctg                 <  2:6372786/150‑1 (MQ=255)
                                                                                                                                        gtcgtAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGAc               >  2:3012430/1‑150 (MQ=255)
                                                                                                                                            tAACTTAATGTATTTATTTAAAACCCCCCCTTAAAAGAACGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACAttt           <  1:7030283/150‑1 (MQ=255)
                                                                                                                                             aaCTTATTGTTTTTATATAAAATACCCCCTTAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACAtttt          <  1:4720430/150‑1 (MQ=255)
                                                                                                                                             aaCTTAATTTATTTATTTAAAATACCCCCTGAAAAGAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACAtttt          <  2:8177025/150‑1 (MQ=255)
                                                                                                                                              aCTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTc         >  2:2223322/1‑150 (MQ=255)
                                                                                                                                              aCTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTc         >  1:1617455/1‑150 (MQ=255)
                                                                                                                                                 tAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGt      >  1:1237937/1‑150 (MQ=255)
                                                                                                                                                     gTATTTATTTAAAATACCCCCTTAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  <  2:2437180/150‑1 (MQ=255)
                                                                                                                                                     gTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  <  2:5201624/150‑1 (MQ=255)
                                                                                                                                                     gTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  <  2:5085396/150‑1 (MQ=255)
                                                                                                                                                     gTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  <  2:4438735/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTTGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGA  >  NC_000913/580578‑580876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: