Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 657,533 A→T 5.3% intergenic (+32/+22) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,5330AT 5.3% 1686.6 / 54.9 472intergenic (+32/+22)cspE/flcconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base A (250/195);  new base T (17/9);  total (267/205)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 473 total aligned reads displayed.

CACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCAC  >  NC_000913/657385‑657680
                                                                                                                                                    |                                                                                                                                                    
cacTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                                                                                                                                                     <  2:3910284/150‑1 (MQ=255)
   ttCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                                                                                                                                                  >  1:3694295/1‑150 (MQ=255)
   ttCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                                                                                                                                                  >  2:4201521/1‑150 (MQ=255)
      tctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgg                                                                                                                                               >  1:1037148/1‑150 (MQ=255)
        tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGACTTCAAAACCCGCTGAATCAGCGGGt                                                                                                                                            >  1:1952541/1‑150 (MQ=255)
        tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                                                                            >  1:8494015/1‑150 (MQ=255)
        tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                                                                            >  2:1640919/1‑150 (MQ=255)
        tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                                                                            >  1:3278266/1‑150 (MQ=255)
          cAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttt                                                                                                                                          >  1:1535705/1‑150 (MQ=255)
             tCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                                                                       >  1:5928115/1‑150 (MQ=255)
               cAGACTACTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATGCGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                                                                     <  1:8081799/150‑1 (MQ=255)
                aGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                                                                    <  1:23851/150‑1 (MQ=255)
                     aaTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCtt                                                                                                                               <  1:202679/150‑1 (MQ=255)
                       tGGTTTTAAAACTCTTGCTGCAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAATACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTa                                                                                                                             <  1:8634549/150‑1 (MQ=255)
                         gTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgt                                                                                                                           <  2:666885/150‑1 (MQ=255)
                         gTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgt                                                                                                                           <  2:7737351/150‑1 (MQ=255)
                           tttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCTCTTAATCAGCGGGTTTTTTTTGGTCTTTAgtgt                                                                                                                         >  1:436791/1‑150 (MQ=255)
                            ttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGtgtg                                                                                                                        >  1:6018402/1‑150 (MQ=255)
                            tacacaCTCTGGCTGAAGGTCAGCGCGTAGAGTTCGAACTCACGAACGGGGCCAACGGCCCTTCTGCTGCAAACGTCATCGCTCTGTAAGATACGTCAGCAAGAATTCACAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGtgtg                                                                                                                        <  2:575208/145‑1 (MQ=255)
                                   tctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGa                                                                                                                 >  1:1133765/1‑150 (MQ=255)
                                   tctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGa                                                                                                                 <  1:6793976/150‑1 (MQ=255)
                                     ttGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAgg                                                                                                               <  1:6707537/150‑1 (MQ=255)
                                       gCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTCACGGTGCCAACGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAACCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGcc                                                                                                             <  2:709728/150‑1 (MQ=255)
                                        cTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGTGGTTGAGGCCg                                                                                                            <  2:2675684/150‑1 (MQ=255)
                                        cTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCg                                                                                                            <  2:6018402/150‑1 (MQ=255)
                                         tGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGa                                                                                                           >  1:8143244/1‑150 (MQ=255)
                                              gTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa                                                                                                      <  1:2118178/150‑1 (MQ=255)
                                                cAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGc                                                                                                    <  2:8610286/150‑1 (MQ=255)
                                                 aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                                                                   <  1:7319056/150‑1 (MQ=255)
                                                  gcgcGTAGAGTTCGAACTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGGACTCGCTCTGTACGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTGGTGTGGTCGTTAGGGTGCGGTTGAGGCCGAAACCAGCCa                                                                                                  >  2:6421136/1‑150 (MQ=255)
                                                      gTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAAt                                                                                              >  2:5234864/1‑150 (MQ=255)
                                                      gTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAAt                                                                                              <  2:6121599/150‑1 (MQ=255)
                                                           gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg                                                                                         >  1:6415959/1‑150 (MQ=255)
                                                            ttCGAAATCACTATCGGTGCCTAAGGCCCTTCTTCTGCTAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAATTCCAGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGt                                                                                        <  2:5540153/150‑1 (MQ=255)
                                                            ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGt                                                                                        >  1:8276687/1‑150 (MQ=255)
                                                            ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGt                                                                                        >  2:3810363/1‑150 (MQ=255)
                                                            ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGt                                                                                        >  2:8338092/1‑150 (MQ=255)
                                                                  aTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTc                                                                                  <  2:6853918/150‑1 (MQ=255)
                                                                   tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCa                                                                                 >  1:4717189/1‑150 (MQ=255)
                                                                    cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCAt                                                                                <  2:1985752/150‑1 (MQ=255)
                                                                     aCTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAACCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                                                                               <  1:7231758/150‑1 (MQ=255)
                                                                       tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                                                                             >  2:1422203/1‑150 (MQ=255)
                                                                       tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                                                                             >  2:1177017/1‑150 (MQ=255)
                                                                       tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                                                                             >  1:8307449/1‑150 (MQ=255)
                                                                        aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCa                                                                            <  2:2338994/150‑1 (MQ=255)
                                                                          cGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa                                                                         <  1:3905590/150‑1 (MQ=255)
                                                                           ggTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa                                                                         >  1:7433587/1‑150 (MQ=255)
                                                                            gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                                                                        >  1:6296662/1‑150 (MQ=255)
                                                                             tGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGa                                                                       <  2:7194389/150‑1 (MQ=255)
                                                                               ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                                                                     >  1:1593099/1‑150 (MQ=255)
                                                                                cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTACTCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                                                                    <  2:2144929/150‑1 (MQ=255)
                                                                                cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                                                                    <  2:8483331/150‑1 (MQ=255)
                                                                                 aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                                                                   >  1:4039222/1‑150 (MQ=255)
                                                                                 aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                                                                   >  2:455035/1‑150 (MQ=255)
                                                                                    ggCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGa                                                                <  2:361151/150‑1 (MQ=255)
                                                                                     gCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaa                                                               <  1:2749765/150‑1 (MQ=255)
                                                                                     gCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaa                                                               <  2:588933/150‑1 (MQ=255)
                                                                                     gCCATTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCTCTTAATCAGAGGGTTTTTTTTGGTCTTTAGTGTGCGGTAGAGGCCGAAAACAGCCATAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaa                                                               >  2:3212287/1‑150 (MQ=255)
                                                                                       ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAgg                                                             >  1:936223/1‑150 (MQ=255)
                                                                                       ccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAgg                                                             >  1:292810/1‑150 (MQ=255)
                                                                                          tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg                                                          >  2:7877106/1‑150 (MQ=255)
                                                                                           ctgctgCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGAAAAAGACCCCAGAAGGTTGa                                                         <  1:2189728/150‑1 (MQ=255)
                                                                                               tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAATCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaa                                                     >  2:2903152/1‑150 (MQ=255)
                                                                                               tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaa                                                     >  1:754933/1‑150 (MQ=255)
                                                                                               tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaa                                                     >  2:2402920/1‑150 (MQ=255)
                                                                                                gcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaa                                                    >  1:3979746/1‑150 (MQ=255)
                                                                                                   aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                 >  1:4983062/1‑150 (MQ=255)
                                                                                                      gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc                                              <  1:3528251/150‑1 (MQ=255)
                                                                                                      gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc                                              <  1:3139858/150‑1 (MQ=255)
                                                                                                         aTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGt                                           <  1:6954523/150‑1 (MQ=255)
                                                                                                           cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTaa                                         >  2:798882/1‑150 (MQ=255)
                                                                                                            gCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAAt                                        <  1:1366019/150‑1 (MQ=255)
                                                                                                             ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATg                                       >  2:5465243/1‑150 (MQ=255)
                                                                                                              tctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGc                                      >  1:5265451/1‑150 (MQ=255)
                                                                                                                tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGcc                                     >  1:3387382/1‑150 (MQ=255)
                                                                                                                tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGt                                                                                        <  2:6759699/98‑1 (MQ=255)
                                                                                                                  tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAg                                  >  2:779414/1‑150 (MQ=255)
                                                                                                                    aGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGcc                                <  2:3342983/150‑1 (MQ=255)
                                                                                                                    aGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGcc                                <  1:4201521/150‑1 (MQ=255)
                                                                                                                      aTACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTACAGTAATGCCCAGCaaa                              >  1:7007028/1‑150 (MQ=255)
                                                                                                                          gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCg                          <  2:8143244/150‑1 (MQ=255)
                                                                                                                               cAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCCTGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct                     >  1:6757702/1‑150 (MQ=255)
                                                                                                                               aaaGAATTAAAACCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct                     <  1:5494917/149‑1 (MQ=255)
                                                                                                                                  gAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCtt                  <  2:4962539/150‑1 (MQ=255)
                                                                                                                                  gAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCtt                  >  2:4357928/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGt                >  1:4579611/5‑150 (MQ=255)
                                                                                                                                    aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGt                >  1:3494913/5‑150 (MQ=255)
                                                                                                                                    aTTCACAACCGGCTTTATCAGGGGTTTTTTTTTGGTCTTTCGTGTGGGGTTGAGGCGTAAAACAGCCCGAATGCCAGTGGGGTCATGGCAAATGACCCCAGACGGTGGACGAACCCGTTCAGTATTGCCCAGCCAAAGCGGCCCTCTTGt                <  1:7769532/150‑1 (MQ=255)
                                                                                                                                      tCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCATAATGCCAGTGCGGTCATGGAAAAAGACCCCAGAAGGTTGACGAAAACGTTCTGTAATGCCCAGCCAAAGCGGCCCTCTTGTaa              <  1:5863215/150‑1 (MQ=255)
                                                                                                                                        aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACa            >  1:5721118/1‑150 (MQ=255)
                                                                                                                                        aaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAAc                                                  >  2:7588308/1‑112 (MQ=255)
                                                                                                                                         aaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaa           <  1:909231/150‑1 (MQ=255)
                                                                                                                                           aCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaa         >  1:6191434/1‑150 (MQ=255)
                                                                                                                                           aCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa                                                                         >  2:1579721/1‑86 (MQ=255)
                                                                                                                                            cccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaaa        >  1:7534679/1‑150 (MQ=255)
                                                                                                                                            cccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaaa        <  2:3507362/150‑1 (MQ=255)
                                                                                                                                             ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAac       >  2:8094941/1‑150 (MQ=255)
                                                                                                                                              cGCTTAATCAGCGGGTTTTTTTTGGTCTTTTGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaaa        <  1:841476/148‑1 (MQ=255)
                                                                                                                                              cGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAaca      <  1:7111240/150‑1 (MQ=255)
                                                                                                                                                 ttAATCAGGGGGTTTTTTTTGGTCTTTTGTGTGCGGTTGTGGCCGAAAACTGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGATGGTTGACGAAAACGTTATTTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcacca   <  1:7998691/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCAC  >  NC_000913/657385‑657680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: