Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 151,688 C→G 10.7% V182L (GTA→CTA)  yadL ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913151,6880CG10.7% 1423.9 / 34.4 459V182L (GTA→CTA) yadLputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (248/153);  new base G (2/53);  total (251/208)
Fisher's exact test for biased strand distribution p-value = 4.50e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

AAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGA  >  NC_000913/151539‑151837
                                                                                                                                                     |                                                                                                                                                     
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                       >  1:6904178/1‑150 (MQ=255)
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                       >  2:1595490/1‑150 (MQ=255)
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                       >  2:1701519/1‑150 (MQ=255)
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                       <  2:52252/150‑1 (MQ=255)
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                       <  2:4067655/150‑1 (MQ=255)
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGGTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCGGTTAGGTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACAGTGTTTAc                                                                                                                                                       >  2:8285423/1‑150 (MQ=255)
 aaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACg                                                                                                                                                      >  1:3682597/1‑150 (MQ=255)
     aGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGttt                                                                                                                                                  >  2:7274701/1‑150 (MQ=255)
        aaTGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAt                                                                                                                                               <  2:1497239/150‑1 (MQ=255)
         aTGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAtt                                                                                                                                              >  2:6409053/1‑150 (MQ=255)
            acagaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttt                                                                                                                                           >  2:2183335/1‑150 (MQ=255)
               gaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTc                                                                                                                                        <  2:1204240/150‑1 (MQ=255)
               gaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTc                                                                                                                                        <  1:2915239/150‑1 (MQ=255)
                aaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTcc                                                                                                                                       <  1:1001653/150‑1 (MQ=255)
                 aacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCa                                                                                                                                      <  2:2452406/150‑1 (MQ=255)
                 aacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCa                                                                                                                                      <  1:4825578/150‑1 (MQ=255)
                   cagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACg                                                                                                                                    <  2:6852864/150‑1 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   >  2:5575753/1‑150 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   >  1:1786973/1‑150 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACACGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   >  2:6777079/1‑150 (MQ=255)
                      aaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCAACGAcc                                                                                                                                 >  1:4106855/1‑150 (MQ=255)
                        ttattCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACTTTGTTTACGGTTTTATTTTTTTCCACGACCAg                                                                                                                               <  2:700017/150‑1 (MQ=255)
                           ttCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCg                                                                                                                            >  1:1166367/1‑150 (MQ=255)
                            tctcTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGt                                                                                                                           <  2:4710980/150‑1 (MQ=255)
                            tctcTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGt                                                                                                                           <  1:2260564/150‑1 (MQ=255)
                             ctctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTg                                                                                                                          <  1:6205537/150‑1 (MQ=255)
                              tctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc                                                                                                                         >  1:2450173/1‑150 (MQ=255)
                              tctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc                                                                                                                         >  1:6248960/1‑150 (MQ=255)
                              tctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc                                                                                                                         >  1:5988484/1‑150 (MQ=255)
                                   atcaGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCa                                                                                                                    >  1:8078086/1‑150 (MQ=255)
                                     caGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATc                                                                                                                  <  1:8573040/150‑1 (MQ=255)
                                      aGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCg                                                                                                                 <  1:4945854/150‑1 (MQ=255)
                                      aGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCg                                                                                                                 <  1:7225398/150‑1 (MQ=255)
                                      aGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCg                                                                                                                 <  1:5416927/150‑1 (MQ=255)
                                        cTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGa                                                                                                               >  2:4210920/1‑150 (MQ=255)
                                         ttACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGat                                                                                                              <  2:3644067/150‑1 (MQ=255)
                                             gTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataata                                                                                                          >  2:7090938/1‑150 (MQ=255)
                                              ttGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                         >  2:6661654/1‑150 (MQ=255)
                                              ttGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                         >  2:3149977/1‑150 (MQ=255)
                                                gAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAAc                                                                                                       >  1:5759193/1‑150 (MQ=255)
                                                 aGTGGGATGCATATATAGTTCCTGAATCTGTAAGTATGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACg                                                                                                      >  1:2354104/1‑150 (MQ=255)
                                                      gATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACACATCGGATAATAAACGACCGc                                                                                                 >  2:8532868/1‑150 (MQ=255)
                                                          cATATATCGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAGGGTTTTATTTTTTTCCACGACCAGGGGTGCACTCATCGGATAATAAACGACCGCATTg                                                                                             <  2:607726/150‑1 (MQ=255)
                                                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCt                                                                                          >  2:7501241/1‑150 (MQ=255)
                                                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGGGGCTGGTGCATAAAACTTACCCGCAGTTACATTGTTTAGGGTTTTATTTTTTTCCACGACCTGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCt                                                                                          <  2:1925633/150‑1 (MQ=255)
                                                              tataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTg                                                                                         >  1:155838/1‑150 (MQ=255)
                                                                taGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCa                                                                                       >  2:102301/1‑150 (MQ=255)
                                                                  gTTCCTGAATCTTTAAGTTTTTTAGTTATTGTTTACTGTAATTGTGGCTGTTGCATAAAAATTACCCGCCGTGAATTTTTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATGGGTTAATAAACGACCGCATTGGCTGCAGt                                                                                     <  2:2348294/150‑1 (MQ=255)
                                                                   ttCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTg                                                                                    >  1:6330139/1‑150 (MQ=255)
                                                                    tCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATCATAAACGACCGCATTGGCTGCAGTgg                                                                                   >  2:5697189/1‑150 (MQ=255)
                                                                     ccTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGga                                                                                  <  2:2108729/150‑1 (MQ=255)
                                                                      cTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgag                                                                                 >  2:6288146/1‑150 (MQ=255)
                                                                       tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgaga                                                                                >  1:2581176/1‑150 (MQ=255)
                                                                       tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgaga                                                                                >  1:4262857/1‑150 (MQ=255)
                                                                           tCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGt                                                                            >  1:3312821/1‑150 (MQ=255)
                                                                             tGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                          >  1:8321440/1‑150 (MQ=255)
                                                                              gTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTAc                                                                         >  2:3768703/1‑150 (MQ=255)
                                                                                    cttttttttaTTGGTTACTGTAAATGTGGCTGGTGCATAAAACTTACCCGCTGTGACATTTTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCAATCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtg                                                                   <  2:1773281/143‑1 (MQ=255)
                                                                                       ttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGa                                                                >  1:3843343/1‑150 (MQ=255)
                                                                                         agttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATg                                                              >  1:7465824/1‑150 (MQ=255)
                                                                                              taGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgt                                                         <  2:614869/150‑1 (MQ=255)
                                                                                              taGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACACGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgt                                                         >  2:5094436/1‑150 (MQ=255)
                                                                                                ggTTACTTTAATTGTGGCTGGTGCACAAAACTCCCCCGCTGTTACATTGTTTAGGGTTTTTTTTTTTCCCCCGACCTGGCGTGCACACATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgtgt                                                       <  1:5606191/150‑1 (MQ=255)
                                                                                                 gTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGtt                                                      >  2:5612009/1‑150 (MQ=255)
                                                                                                 gTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGtt                                                      >  2:7775899/1‑150 (MQ=255)
                                                                                                 gTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGtt                                                      >  2:497295/1‑150 (MQ=255)
                                                                                                  ttACTGTAATTGTGGCTGGTGAATAAAACTTCCCCGCAGTGACTTTTTTTAGGTTTTTATTTTTTCCCCCGACCTGGGGTGCACCCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTa                                                     <  2:3926547/150‑1 (MQ=255)
                                                                                                  ttACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTa                                                     >  1:4739923/1‑150 (MQ=255)
                                                                                                  ttACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTa                                                     >  1:506032/1‑150 (MQ=255)
                                                                                                   tACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTac                                                    <  2:2741905/150‑1 (MQ=255)
                                                                                                      tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                 >  2:2903828/1‑150 (MQ=255)
                                                                                                        tAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGc                                               >  1:1641825/1‑150 (MQ=255)
                                                                                                         aattgtgGCTGGTGAACAAACCTTCCCCCCAGTCACTTTTTTTAGGGTTTTATTTTTTTCCACCCCCCGGCGTGAACTCCTCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCt                                              <  1:5132627/147‑1 (MQ=255)
                                                                                                          aaTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTa                                             >  1:661266/1‑150 (MQ=255)
                                                                                                             gtgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAAt                                          >  1:1912947/1‑150 (MQ=255)
                                                                                                              tgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAAtt                                         >  1:458641/1‑150 (MQ=255)
                                                                                                                gggggTGCAGAAAACTTCCCCGCCGTGACTTTGTTTCGGGTTTTATTTTTGGCCCCGACCCGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgt                                       <  2:7456513/147‑1 (MQ=255)
                                                                                                                gcgggTGCAGAAATCTTACCCGCAGTGACTTTTTTTACGTTTTTATTTTTTCCCACGACCCGGCGTGCACACTTCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgt                                       <  1:7688643/147‑1 (MQ=255)
                                                                                                                  tGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGAGTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTgtgt                                     >  2:5885926/1‑150 (MQ=255)
                                                                                                                   gttGCAGAAAATTTCCCCGCATTTACTTTGTTTTGGTTTTTTTTTTTTTCCACGACCCGGGGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGtt                                    <  1:8706421/148‑1 (MQ=255)
                                                                                                                      gCAGAAAACTTACCCGCAGTGACATTGTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCa                                 <  2:4829433/150‑1 (MQ=255)
                                                                                                                       cAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCAc                                >  2:8098948/1‑150 (MQ=255)
                                                                                                                        aGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACt                               >  2:7942819/1‑150 (MQ=255)
                                                                                                                          aaaaCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTcc                             <  2:544510/150‑1 (MQ=255)
                                                                                                                           aaaCTTACCCGCTGGGACTTTGTTTCGGGTTTTATTTTTTTCCACGACCCGGGGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTccc                            <  1:7090938/150‑1 (MQ=255)
                                                                                                                            aaCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCt                           >  1:5169360/1‑150 (MQ=255)
                                                                                                                               ttACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGtt                        >  1:4432508/1‑150 (MQ=255)
                                                                                                                               ttACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGtt                        >  1:3609298/1‑150 (MQ=255)
                                                                                                                                 aCCCGCTGTGACATTTTTTCCGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGc                      <  2:4604288/150‑1 (MQ=255)
                                                                                                                                  cccGCTGTTACTTTGTTTCGGTTTTTATTTTTTTCCCCGACCCGGCGTGCACTCATGGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                     <  1:3230323/150‑1 (MQ=255)
                                                                                                                                  cccGCAGTGACATTGTTTCCGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                     <  2:3523600/150‑1 (MQ=255)
                                                                                                                                   ccGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                    >  2:3357921/1‑150 (MQ=255)
                                                                                                                                   ccGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                    >  1:5226273/1‑150 (MQ=255)
                                                                                                                                     gCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                  >  2:5473754/1‑150 (MQ=255)
                                                                                                                                           actttttttAGGTTTTTTTTTTTTTCCACGACCAGGCGTGCACTCATCGGATAACAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa            <  2:3850058/144‑1 (MQ=255)
                                                                                                                                           actttGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa            <  2:8623845/147‑1 (MQ=255)
                                                                                                                                           aCATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa            >  1:3052659/1‑150 (MQ=255)
                                                                                                                                             aTTGTTTACGGTTTTATTTTTTTCCACGACCCGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGt          <  1:7817091/150‑1 (MQ=255)
                                                                                                                                                   tACGTTTTTATTTTTTTCCACGACCAGGCGTGCACACATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCa    <  1:2763839/150‑1 (MQ=255)
                                                                                                                                                   tACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTAATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCa    <  2:4389462/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGA  >  NC_000913/151539‑151837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: