Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 784,961 G→T 10.1% T119T (ACC→ACA ybgS ← putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913784,9610GT10.1% 1239.2 / 25.0 418T119T (ACC→ACAybgSputative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (147/215);  new base T (53/3);  total (200/218)
Fisher's exact test for biased strand distribution p-value = 1.25e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 431 total aligned reads displayed.

TGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGT  >  NC_000913/784812‑785110
                                                                                                                                                     |                                                                                                                                                     
tgagtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTg                                                                                                                                                       <  1:6964386/150‑1 (MQ=255)
tgagtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTg                                                                                                                                                       <  2:4239866/150‑1 (MQ=255)
 gagtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTgg                                                                                                                                                      <  2:1192744/150‑1 (MQ=255)
 gagtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTGTTgg                                                                                                                                                      >  1:1723815/1‑150 (MQ=255)
     gcgcCATGACGAGTCATCCCTTAAGTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATc                                                                                                                                                  <  2:2330723/150‑1 (MQ=255)
        ccATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAc                                                                                                                                               <  1:7186636/150‑1 (MQ=255)
         cATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAca                                                                                                                                              <  1:1343072/150‑1 (MQ=255)
         cATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAca                                                                                                                                              <  2:343153/150‑1 (MQ=255)
         cATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAca                                                                                                                                              >  1:4862181/1‑150 (MQ=255)
               gAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCatta                                                                                                                                        <  1:8028840/150‑1 (MQ=255)
                  tCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCAATATTg                                                                                                                                     >  2:3984240/1‑150 (MQ=255)
                   cATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGa                                                                                                                                    <  2:8564391/150‑1 (MQ=255)
                    aTCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                   <  1:8136897/150‑1 (MQ=255)
                    aTCCCTTAATTAGTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTCTTGGTATCGACATCATTATTGAt                                                                                                                                   <  1:7803225/150‑1 (MQ=255)
                     tCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTCCCGTCGGGGTTGGTATCGACAACAATATTGATg                                                                                                                                  >  2:8011184/1‑150 (MQ=255)
                     tCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATg                                                                                                                                  <  2:559122/150‑1 (MQ=255)
                     tCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCAATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATg                                                                                                                                  >  2:4992505/1‑150 (MQ=255)
                      cccTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGc                                                                                                                                 <  1:3891538/150‑1 (MQ=255)
                       ccTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACATCATTATTGATGcc                                                                                                                                >  1:3311955/1‑150 (MQ=255)
                        cTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCa                                                                                                                               >  1:2473579/1‑150 (MQ=255)
                         ttAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACAACCTTATTTATGCCaa                                                                                                                              >  2:5731034/1‑149 (MQ=255)
                           aaTTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCa                                                                                                                            <  2:829793/150‑1 (MQ=255)
                             ttattCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCAcc                                                                                                                          <  2:734673/150‑1 (MQ=255)
                                ttCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTTATTCCATCaaccgt                                                                                                                       >  2:7001012/1‑145 (MQ=255)
                                ttCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGt                                                                                                                       <  1:8369189/150‑1 (MQ=255)
                                 tctcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGtt                                                                                                                      <  2:3576839/150‑1 (MQ=255)
                                  ctcACTTACGTTTCTGACATGATTCTGATTTTAGATGTTAATTTTTTAAAGGGAGAGACTCCGCTCTCCCTTTTTTTGCTATTTTGCTGGTTTACTGCGTGGTTCCGTCGGTTTTTGTATCGACTTCATTATTTATGCCATCACCGGttt                                                                                                                     <  2:1718393/150‑1 (MQ=255)
                                  ctcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGttt                                                                                                                     <  1:7041892/150‑1 (MQ=255)
                                   tcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTTATGGCAACACCGGtttt                                                                                                                    >  1:5889735/1‑149 (MQ=255)
                                    cACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGt                                                                                                                   <  2:2006158/150‑1 (MQ=255)
                                     aCTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTa                                                                                                                  <  2:3272965/150‑1 (MQ=255)
                                       ttAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTTATGCCAACACCGGTTTGTAcc                                                                                                                >  1:7460818/1‑150 (MQ=255)
                                          aCTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATTCCATCACCGGGTTTTaacatt                                                                                                             >  1:3724518/1‑145 (MQ=255)
                                          aCTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCttt                                                                                                             <  2:7516619/150‑1 (MQ=255)
                                             ttATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTa                                                                                                          <  1:2197604/150‑1 (MQ=255)
                                              tATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAt                                                                                                         <  2:186326/150‑1 (MQ=255)
                                               aTGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                                        <  1:5406922/150‑1 (MQ=255)
                                               aTGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAtt                                                                                                        <  1:2063473/150‑1 (MQ=255)
                                                    atcatACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTCGTGGCGATTTTGCAGGGTTACGGCGTGGTCCCGTCGGGTTTGGGATCGACATCCGTCTGGAGGGCCTCACCGGGGTGGGCCTTTTTTTTgtgcg                                                                                                   >  2:5387701/1‑146 (MQ=255)
                                                     tcatACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTCCTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATc                                                                                                  <  2:6422910/150‑1 (MQ=255)
                                                     tcatACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATGCCATCACCCGTTTTTACCTTTTTATTTatata                                                                                                  >  1:8642236/1‑149 (MQ=255)
                                                     tcatACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACAACAATATTGATGCCATCAACCGTTTGTAACATTTTAttttaatc                                                                                                  >  2:1529127/1‑144 (MQ=255)
                                                       atACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTTATGCCAACACCGGTTTTTACCTTTTTATTTatataag                                                                                                >  2:5922189/1‑147 (MQ=255)
                                                       atACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCg                                                                                                <  1:4182095/150‑1 (MQ=255)
                                                          cTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCAATATTTATGCCCACACCGGTTTGTACCATTTTATTTATATACGGAc                                                                                             >  1:1578623/1‑150 (MQ=255)
                                                           tGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACAACAATATTGAAGCCATCACCCGGTTGTTCCATTTTATTTATATCCGGGCa                                                                                            >  2:2526484/1‑150 (MQ=255)
                                                           tGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTTTCGACATCAATATTGATGCCCTCACCCGGTTGTACCATTTTATTTATATCCGGACa                                                                                            >  2:1527129/1‑150 (MQ=255)
                                                            gCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTCATTGATATCCGGACAg                                                                                           <  1:1590001/150‑1 (MQ=255)
                                                            gCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTTGTATCGACATACTTATTTTTTCCCACACCCGTTTTTAACATTTTATTTTTATACGGCCAg                                                                                           >  2:6578602/1‑150 (MQ=255)
                                                                  aGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTAACTTTTTATTGTTATACGGACAGCGACCa                                                                                     >  1:6441092/1‑150 (MQ=255)
                                                                    aaGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTGATGCCATCACCGGGTTGTAACTTTTTATTTATATCCGGACAGCGACCaac                                                                                   >  2:5154840/1‑148 (MQ=255)
                                                                    aaGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATc                                                                                   <  2:7779231/150‑1 (MQ=255)
                                                                       tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTTTTCCATCACCCGTTTGTAACTTTTTATTGATATAAGGACAGCGACCATCttt                                                                                >  1:1882462/1‑150 (MQ=255)
                                                                       tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCttt                                                                                <  1:8312768/150‑1 (MQ=255)
                                                                       tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCttt                                                                                <  2:3719543/150‑1 (MQ=255)
                                                                       tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATAATTATTGATGCCAGCACCCGGTTGTACCTTTTTATTGTTATCCGGACAGCGACCATCttt                                                                                >  1:4018359/1‑150 (MQ=255)
                                                                         aaaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGCCATCAATATTGATTCCCGCACCCGGTTGTACCATTTTATTTATATCCGGACAGCGCCCATCGTTGc                                                                              >  2:8154714/1‑150 (MQ=255)
                                                                             aTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACAt                                                                          <  2:4119777/150‑1 (MQ=255)
                                                                             aTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCAACACCCGTGTGTACCTTTTTATTGATATCCGGCCAGCGACCATCGTTGcacag                                                                          >  2:7485821/1‑149 (MQ=255)
                                                                               tAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTTATATCCGGACAGCGACCAACTTTGCACATgg                                                                        >  2:4576029/1‑150 (MQ=255)
                                                                                aaaaGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTAAAGACATAAATAATTATTCCAACAACGGTTTTTACATTTTTATTTATATCCGGACAGCGACAAACTATGcacaatgt                                                                       >  2:2633195/1‑146 (MQ=255)
                                                                                 aaaGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGGAAAGACAGCATTATTTATGCCAACACCCGTGTGTACCATTTTAATGATATCACGACAGCGACCATCTTTTCACATTgtt                                                                      >  2:7309197/1‑149 (MQ=255)
                                                                                  aaGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCCTCTTTGCACATTgtgt                                                                     >  1:4984439/1‑150 (MQ=255)
                                                                                    gggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTc                                                                   <  1:4090814/150‑1 (MQ=255)
                                                                                    gggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTc                                                                   <  1:5830016/150‑1 (MQ=255)
                                                                                     ggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACAACATTATTGATGCCATAACCCGGTTGTACCTTTTTATTTATATCCGGACAGCGACCATATTTTCACAAGGTGTTCt                                                                  >  1:7342557/1‑150 (MQ=255)
                                                                                      gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                 <  2:6974727/150‑1 (MQ=255)
                                                                                      gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                 <  2:1389099/150‑1 (MQ=255)
                                                                                      gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACAGCATTATTGATGCCATCACCGGGTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                 >  1:5656474/1‑150 (MQ=255)
                                                                                        gagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgt                                                               <  1:2743621/150‑1 (MQ=255)
                                                                                          gaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGc                                                             <  1:5979103/150‑1 (MQ=255)
                                                                                           aCTCCGCTCTCCCAGTATTGGCGAGTTGGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGct                                                            <  1:2366921/150‑1 (MQ=255)
                                                                                             tCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATGCCATCACCGGTTTGTAACTTTTTATTGATATACGGACAGCGACCATCTTTGCACAAGGTGTTCTTTTGgtct                                                          >  1:5317028/1‑150 (MQ=255)
                                                                                             tCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACAACCATATTGATGCCATCACCCGGGTGTACCATTTTATTGATATACCGACAGCGACCAGCTTTGCACAAGGTGTTCGTGTGctcg                                                          >  1:5132142/1‑149 (MQ=255)
                                                                                              ccGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTTCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTGATGCCTTCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGTCCATCTTTGCACATGGTGTTCTTGTGCTCtt                                                         <  2:1754050/150‑1 (MQ=255)
                                                                                                  tctcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCCGTTTGTACCTTTTTATTGATATCCGGACACCGACCATCTATGCACATGGTGTTCTTGTGCTCTTCATc                                                     >  2:7621608/1‑150 (MQ=255)
                                                                                                    tcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCtt                                                   <  2:440846/150‑1 (MQ=255)
                                                                                                     cccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCCTTGCACATGGTGTTCTTGTGCTCTTCATCttt                                                  <  1:4292340/150‑1 (MQ=255)
                                                                                                      ccATTATTGGCTATTTTGCAGTGTTACTGCGTTGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTg                                                 <  1:7085735/150‑1 (MQ=255)
                                                                                                       cATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTgg                                                <  2:1291542/150‑1 (MQ=255)
                                                                                                        attattGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGt                                               <  1:3563540/150‑1 (MQ=255)
                                                                                                         ttattGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATTCCATCACCGGTTTGTACCTTTTTATTTATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTTTGCTCTTCATCTTTTGTc                                              >  2:4804885/1‑150 (MQ=255)
                                                                                                            ttGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCAtt                                           <  2:2822330/150‑1 (MQ=255)
                                                                                                             tGGCTATTTTGCAGGGTTACTGCGTGGTAACGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTc                                          <  2:2466201/150‑1 (MQ=255)
                                                                                                                 tATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTACGTCa                                     >  2:6360646/1‑150 (MQ=255)
                                                                                                                 tATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACAACATTATTGATGCCATCACCGGTTTGTACCTTTTTAATGATATCCGGACAGCGACCCTCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTc                                      >  2:8680963/1‑150 (MQ=255)
                                                                                                                    tttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAtt                                   <  2:3040821/150‑1 (MQ=255)
                                                                                                                        cAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTc                               <  1:2973279/150‑1 (MQ=255)
                                                                                                                           ggTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCCTTTTTGATGCCATCACCGGTTTGTACCATTTTATTGATATACCGACAGCGACCATCTTTTCACATGGTGTTCTTGTGCTCTTCAACATTTGTCATTCCGTCAATTTTAAtt                            >  2:2380494/1‑150 (MQ=255)
                                                                                                                           ggTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCAtt                            <  1:4651856/150‑1 (MQ=255)
                                                                                                                              tACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTgaa                         <  2:4298379/150‑1 (MQ=255)
                                                                                                                              tACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTgaa                         <  1:7574052/150‑1 (MQ=255)
                                                                                                                                     tGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCa                                                       <  1:1024938/113‑1 (MQ=255)
                                                                                                                                          ccGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGaa             <  1:914729/150‑1 (MQ=255)
                                                                                                                                           cGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACAATCTTTGAACATGGTGTTCTTGTGATCTTCATCTTTGGTCATTCCGTCATTGTTCATTTAAGAACCATCCGAAt            >  2:5331325/1‑150 (MQ=255)
                                                                                                                                            gTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTTATATCCGGACAGCGACCATGTTTGCACATGGTGTTCTTGTGGTCTTCTTCTTTGGTCATTCCGTCATTGTTGATTGTAGATCCTTCCGAATg           >  2:8493178/1‑150 (MQ=255)
                                                                                                                                                gTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagc       >  2:6285662/1‑150 (MQ=255)
                                                                                                                                                 ttttGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAt                                    >  2:2271251/1‑120 (MQ=255)
                                                                                                                                                 ttttGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAt                                    <  1:6628139/120‑1 (MQ=255)
                                                                                                                                                     ggTATCGAAATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCAGACAGCGACAATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATACGAATGCAGCATtgt  <  2:8350971/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGT  >  NC_000913/784812‑785110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: