Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 784,979 G→T 6.5% I113I (ATC→ATA ybgS ← putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913784,9790GT 6.5% 1411.9 / 5.8 438I113I (ATC→ATAybgSputative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (172/220);  new base T (44/2);  total (216/222)
Fisher's exact test for biased strand distribution p-value = 1.00e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 446 total aligned reads displayed.

TCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATT  >  NC_000913/784830‑785128
                                                                                                                                                     |                                                                                                                                                     
tCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTg                                                                                                                                                       <  2:4370390/150‑1 (MQ=255)
tCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATGATTGGCTAGTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGGCTCGACATCATTATTg                                                                                                                                                       >  2:8666280/1‑150 (MQ=255)
  aTCCCTTAATTATTCTCACTTAACTTTCTGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                                     <  1:8136980/150‑1 (MQ=255)
  aTCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                                     >  1:6821594/1‑150 (MQ=255)
  aTCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGAt                                                                                                                                                     <  1:7655971/150‑1 (MQ=255)
    cccTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGc                                                                                                                                                   <  1:3891538/150‑1 (MQ=255)
      cTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTGTCGACATCAATATTGATGCCa                                                                                                                                                 >  1:6411132/1‑150 (MQ=255)
      cTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCa                                                                                                                                                 >  1:2473579/1‑150 (MQ=255)
      cTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACATCATTATTGATGccc                                                                                                                                                 >  1:7058052/1‑149 (MQ=255)
       ttAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCAt                                                                                                                                                >  1:5917467/1‑150 (MQ=255)
       ttAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGGTCCTGCGTGGTCCCGTCGGTGTTGGTAGCGACCTCCATATTGATGcccg                                                                                                                                                >  2:5335917/1‑148 (MQ=255)
         aaTTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCa                                                                                                                                              <  2:4179909/150‑1 (MQ=255)
           ttattCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCAcc                                                                                                                                            >  1:2544619/1‑150 (MQ=255)
            tattCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACAAAAATATTGATTACCTCAccc                                                                                                                                           >  2:2261554/1‑149 (MQ=255)
              ttCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTTATTCCATCaaccgt                                                                                                                                         >  2:7001012/1‑145 (MQ=255)
               tctcACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATGCCATCACCGGtt                                                                                                                                        >  1:5374578/1‑150 (MQ=255)
                  cACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGt                                                                                                                                     <  2:2006158/150‑1 (MQ=255)
                   aCTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTa                                                                                                                                    <  2:7837338/150‑1 (MQ=255)
                     ttAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACAACATTATTTATGCCATCACCCGGTTGTAcc                                                                                                                                  >  1:7460655/1‑150 (MQ=255)
                     ttAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATAGACATCATTATTGATGCCCTCACCGGGGTGTAcc                                                                                                                                  >  2:4287133/1‑150 (MQ=255)
                          tttATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATGCCAGCACCGGGTTGTACCttttt                                                                                                                             >  2:3563540/1‑150 (MQ=255)
                          tttATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTAATGATGCCATCACCGGTTTGTACCttttt                                                                                                                             >  1:7498694/1‑150 (MQ=255)
                          tttATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTCTCGACATAATTATTGATGCCAACACCGGTTTGTACCttttt                                                                                                                             >  2:7667859/1‑150 (MQ=255)
                            tATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTAt                                                                                                                           <  2:186326/150‑1 (MQ=255)
                              tGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTg                                                                                                                         <  2:6380762/150‑1 (MQ=255)
                               gACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGa                                                                                                                        >  1:4298379/1‑150 (MQ=255)
                               gACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGa                                                                                                                        >  1:1933113/1‑150 (MQ=255)
                                   tcatACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTGATGCCATCACCCGTTTTTACCTTTTTATTTatata                                                                                                                    >  1:8642236/1‑149 (MQ=255)
                                     atACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCATTATTTATGCCAACACCGGTTTTTACCTTTTTATTTatataag                                                                                                                  >  2:5922189/1‑147 (MQ=255)
                                     atACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCg                                                                                                                  <  1:4871171/150‑1 (MQ=255)
                                     atACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCg                                                                                                                  <  1:4182095/150‑1 (MQ=255)
                                       aCTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGa                                                                                                                <  2:6177149/150‑1 (MQ=255)
                                        cTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTTATGACATCACCGGTTTGTACCTTTTTATTTATATCCGGAc                                                                                                               >  2:8539794/1‑150 (MQ=255)
                                        cTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCCGTTTGTACCTTTTTATTGATATCCGGAc                                                                                                               >  1:5249263/1‑150 (MQ=255)
                                         tGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACAAAAATAATGATGCCAACACCCGTTTTTACCTTTTTATTGATATACGGaaa                                                                                                              >  2:1527249/1‑148 (MQ=255)
                                          gattttAGAAGTGAAAAATTAAAAGTTAGATACTCCGCTCTCCAATTATTGGATATTTTTCAGGGTTACTGCGTGGTACAGTGGGTTTTTGTTTCTAAATCATTATTGATTCAAACACAGGTTTGTAAAGTTTTATTTATAGACGGACAg                                                                                                             >  2:6578491/3‑150 (MQ=255)
                                          gCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAg                                                                                                             <  1:1589622/150‑1 (MQ=255)
                                           cTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGGTTGTACCTTTTTATTGATATCCGGACAGc                                                                                                            >  1:500767/1‑150 (MQ=255)
                                                  aaGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTGATGCCATCACCGGGTTGTAACTTTTTATTTATATCCGGACAGCGACCaac                                                                                                     >  2:5154840/1‑148 (MQ=255)
                                                  aaGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGat                                                                                                                       <  1:2914225/132‑1 (MQ=255)
                                                     tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCCTTATTTATGCCAGCACCGGGTTGTACCTTTTTATTGATATACGGACAGCGACCCTCttt                                                                                                  >  1:3724470/1‑150 (MQ=255)
                                                     tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCttt                                                                                                  <  1:8312768/150‑1 (MQ=255)
                                                           aTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACAt                                                                                            <  2:3924490/150‑1 (MQ=255)
                                                             tAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTTATATCCGGACAGCGACCAACTTTGCACATgg                                                                                          >  2:4576029/1‑150 (MQ=255)
                                                             tAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACAGCAATATTGATGCCCTCACCGGGTTGTACCTTTTTATTGATATCCGGACACCGACCAACGTTGCACATgg                                                                                          >  2:7574052/1‑150 (MQ=255)
                                                              aaaaGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTAAAGACATAAATAATTATTCCAACAACGGTTTTTACATTTTTATTTATATCCGGACAGCGACAAACTATGcacaatgt                                                                                         >  2:2633195/1‑146 (MQ=255)
                                                               aaaGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGGAAAGACAGCATTATTTATGCCAACACCCGTGTGTACCATTTTAATGATATCACGACAGCGACCATCTTTTCACATTgtt                                                                                        >  2:7309197/1‑149 (MQ=255)
                                                                  gggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTc                                                                                     <  1:3359031/150‑1 (MQ=255)
                                                                  gggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTc                                                                                     <  1:4090814/150‑1 (MQ=255)
                                                                   ggAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATCGACAACATTATTGATGCCATAACCCGGTTGTACCTTTTTATTTATATCCGGACAGCGACCATATTTTCACAAGGTGTTCt                                                                                    >  1:7342557/1‑150 (MQ=255)
                                                                    gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                                   <  2:4814507/150‑1 (MQ=255)
                                                                    gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                                   <  2:6974727/150‑1 (MQ=255)
                                                                    gagagaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACAGCATTATTGATGCCATCACCGGGTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCtt                                                                                   >  1:5656474/1‑150 (MQ=255)
                                                                       agaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgtg                                                                                <  2:6500383/150‑1 (MQ=255)
                                                                       agaCTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTtgtg                                                                                <  2:1530389/150‑1 (MQ=255)
                                                                          cTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGctc                                                                             <  2:3676114/150‑1 (MQ=255)
                                                                           tCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCCACACCGGTTTGTACCATTTTATTGATATCCCGACAGCGACCAACTTTGCACATGGTGTTCTTGTGctct                                                                            >  2:1756815/1‑150 (MQ=255)
                                                                           tCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCAATATTGATTCCATCACCCGGTTGTACCTTTTTATTGATATCCGGACAGCGCCCAACGTTGCACAAGGTGTTCTTGTGctct                                                                            >  1:1221281/1‑150 (MQ=255)
                                                                              gCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCa                                                                         <  1:3999876/150‑1 (MQ=255)
                                                                                  tcCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGTATACACAACATTATTGATGCCAAAAACCGTTTTTACCTTTTTATTTATATACGGACATCGACCAAATTTTCACAAGGTGTTATTTTGCTATTAATAtt                                                                     >  2:4027918/1‑150 (MQ=255)
                                                                                   cccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCttt                                                                    <  1:6966679/150‑1 (MQ=255)
                                                                                    ccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTg                                                                   <  1:7098288/150‑1 (MQ=255)
                                                                                    ccATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGGTTTGGTATCGACATCATTAATTATGCCAACACCGGTTTGTACCATTTTATTTTTATACGGACAGCGACCATCTTTGCAAATTGTGTTGTTGTTCTCTTAAACtttt                                                                   >  1:8445382/1‑149 (MQ=255)
                                                                                     cATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTTGGATAGAAAACAATATTTATGCCATCACCCGTTTTTAACATTTTATTTATATACGGCCAGCGACCAACTTTTCACATTGTTTTCTTTTGCTCTTAATATTTgg                                                                  >  1:21026/1‑150 (MQ=255)
                                                                                      attattGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGt                                                                 <  1:3563540/150‑1 (MQ=255)
                                                                                       ttattGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTTTAGACATAAATATTTATTCCAAAACCCGGTTGTAACTTTTTATTTTTATACGGACAGCGACCAACGTTTCACAAGGTGTTCTTGTTCTCTTAATATTTTGTc                                                                >  1:5880879/1‑150 (MQ=255)
                                                                                          ttGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCAtt                                                             <  2:2822330/150‑1 (MQ=255)
                                                                                                  tttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAtt                                                     <  2:3040821/150‑1 (MQ=255)
                                                                                                  tttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAtt                                                     <  2:8329186/150‑1 (MQ=255)
                                                                                                  tttGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCAtt                                                     <  2:5453287/150‑1 (MQ=255)
                                                                                                       aGGGTTACTGCGTGGTACCGTCGGTTTTGGTATAGACATCATTATTGATTCCAAAACCGGTTTGTAACATTTTATTTATATCCCGACAGCGACCCACTTTGCAAAAGGTTTTATTTTGCTCTTAAACTTTTGTCATTCCGTCATTGTTCa                                                >  2:17214/1‑150 (MQ=255)
                                                                                                         ggTTACTGCGTGGTACCGTCGGTTTTTGTATCGACATCCTTTTTGATGCCATCACCGGTTTGTACCATTTTATTGATATACCGACAGCGACCATCTTTTCACATGGTGTTCTTGTGCTCTTCAACATTTGTCATTCCGTCAATTTTAAtt                                              >  2:2380494/1‑150 (MQ=255)
                                                                                                               tGCGTGGTACCGTCGGTTTTTGTATAGACATCATTATTGATGCCATCACCGGTTTGTACCATTTTATTGATATACGGACCCCGACCAACGTTGCACAGGGTGTTATTGTGCTCTTCATAGTTGGTCATTCCCTCATTGTTCATTgaagaa                                        >  2:2479591/1‑150 (MQ=255)
                                                                                                               tGCGTGGTACCGTCGGGTTTGGTATCGACATCATTATTGGTGCCATCACCGGTTTGTACCATTTTATTGATATCCGGACAGCGACCCACTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTTGTAAATCCGTCATTGTTCATTgaagaa                                        >  2:3314716/1‑150 (MQ=255)
                                                                                                               tGCGTGGTACCGACGGTTTTGGTATAGACCAAATTATTGATTCCCTCACCCGTTTGTACCATTTTATTTATATCCGGACAGCCACCATCTTTGCACATGGTGTTCTTGTTCTCTTAATCTTTGGTCATTCCGTCATTTTTCATTgaagaa                                        >  2:8030876/1‑150 (MQ=255)
                                                                                                                gCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAAc                                       <  2:1431531/150‑1 (MQ=255)
                                                                                                                 cGTGGTACCGTCGGTTTTGGTATCGACATAAATATTGATGCCATCACCCGTTTGTACCATTTTATTTATATACGGACAGCGACCAACTTTGACCATGGTGTTCTTTTTCACTTAATCTTTGGTCATTCCGTAATTGTTCATTgaagaaac                                      >  2:8474225/1‑148 (MQ=255)
                                                                                                                   tGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCa                                                                         <  1:1024938/113‑1 (MQ=255)
                                                                                                                        ccGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGaa                               <  1:914729/150‑1 (MQ=255)
                                                                                                                              gTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagc                         >  2:8175620/1‑150 (MQ=255)
                                                                                                                              gTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATgcagc                         >  2:7506268/1‑150 (MQ=255)
                                                                                                                               ttttGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCAACTTTGCACATGGTGTTCATGTGCTCATCATATTTGGTCATTCCGTAATTGTTCATTTAAGAACAATCCGAATgcagca                        >  1:8039693/1‑150 (MQ=255)
                                                                                                                                 ttGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCAtt                      <  2:1184461/150‑1 (MQ=255)
                                                                                                                                     tATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAGCATCTTTGCTCATGGTGTTGTTGTGCTGTTGATCTTTGGTCATTCGGTCTTTGTTCATTGAAGTACCATCCGAATGCAGCTTTGTgc                  >  2:7421421/1‑150 (MQ=255)
                                                                                                                                     tATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgc                  >  1:4577153/1‑150 (MQ=255)
                                                                                                                                     tATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgc                  <  1:611424/150‑1 (MQ=255)
                                                                                                                                     tATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgc                  <  2:5808505/150‑1 (MQ=255)
                                                                                                                                       tCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTg                                                                   <  1:1469985/99‑1 (MQ=255)
                                                                                                                                        cGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgc               >  2:6314464/1‑150 (MQ=255)
                                                                                                                                        cGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgc               <  1:1866646/150‑1 (MQ=255)
                                                                                                                                        cGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgc               <  2:5616944/150‑1 (MQ=255)
                                                                                                                                         gACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTgccgcc              >  2:3220501/1‑150 (MQ=255)
                                                                                                                                          aCATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCa             <  1:5450089/150‑1 (MQ=255)
                                                                                                                                           catcatTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAg            >  1:2310663/1‑150 (MQ=255)
                                                                                                                                               attattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAAcc        >  2:1318066/1‑150 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg       <  2:7342557/150‑1 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg       <  1:5802930/150‑1 (MQ=255)
                                                                                                                                                ttattGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCg       <  2:5023690/150‑1 (MQ=255)
                                                                                                                                                  attGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGt     >  1:1613234/1‑150 (MQ=255)
                                                                                                                                                  attGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGt     <  1:302685/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACATGGTGTTCTTGTGCTCTTCATCTTTGGTCATTCCGTCATTGTTCATTGAAGAACCATCCGAATGCAGCATTGTGCCGCCAGAACCGGTATT  >  NC_000913/784830‑785128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: