Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,226,115 A→C 5.3% intergenic (‑35/‑485) minC ← / → ycgJ inhibitor of FtsZ ring polymerization/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,226,1150AC 5.3% 1456.9 / 28.3 499intergenic (‑35/‑485)minC/ycgJinhibitor of FtsZ ring polymerization/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (237/227);  new base C (30/5);  total (267/232)
Fisher's exact test for biased strand distribution p-value = 6.45e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 503 total aligned reads displayed.

CCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGTCAATCAACCC  >  NC_000913/1225966‑1226264
                                                                                                                                                     |                                                                                                                                                     
ccTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCa                                                                                                                                                       <  1:5566278/150‑1 (MQ=255)
  tGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAgc                                                                                                                                                     >  2:2738120/1‑150 (MQ=255)
      cGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAgcgcgc                                                                                                                                                 <  1:8402295/150‑1 (MQ=255)
      cGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAgcgcgc                                                                                                                                                 >  1:7509812/1‑150 (MQ=255)
        aTTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCGTACTCAATTAGCTATTAATCATCGCCAGCGCGCga                                                                                                                                               <  1:2420155/150‑1 (MQ=255)
         ttttGTCTTCCAGAGCCTGATGGATAACCTTAGGTTCTTCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGACTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGACAGCGCGCgat                                                                                                                                              >  2:8017989/1‑150 (MQ=255)
           ttGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCGCGCTGGCgatga                                                                                                                                            <  2:4131562/150‑1 (MQ=255)
              tCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGt                                                                                                                                         >  1:2710256/1‑150 (MQ=255)
                ttCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTc                                                                                                                                       >  2:6025365/1‑150 (MQ=255)
                ttCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTc                                                                                                                                       <  1:6849044/150‑1 (MQ=255)
                   cAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGa                                                                                                                                    >  1:251459/1‑150 (MQ=255)
                   cAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGa                                                                                                                                    >  1:3807615/1‑150 (MQ=255)
                    aGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCGCGCTGGCgatgatt                                                                                                                                          >  1:7652155/1‑142 (MQ=255)
                    aGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCGCGCGGGCgatgatt                                                                                                                                          <  2:3329462/143‑2 (MQ=255)
                     gcgcCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGaa                                                                                                                                   >  2:5132606/1‑149 (MQ=255)
                      cgcCTGATGGATAACCTTATGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGaata                                                                                                                                 <  1:5125809/150‑3 (MQ=255)
                      cgcCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGa                                                                                                                                 <  1:2836068/150‑1 (MQ=255)
                      cgcCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGa                                                                                                                                 >  1:4044894/1‑150 (MQ=255)
                        ccTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACt                                                                                                                               <  2:6392070/150‑1 (MQ=255)
                        ccTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACt                                                                                                                               <  1:8188056/150‑1 (MQ=255)
                           gATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACtata                                                                                                                            >  2:3923571/1‑150 (MQ=255)
                           gATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACtata                                                                                                                            <  2:1330505/150‑1 (MQ=255)
                                    ccTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGtt                                                                                                                   >  1:2977398/1‑150 (MQ=255)
                                     cTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTta                                                                                                                  >  2:2996194/1‑150 (MQ=255)
                                           ttCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCt                                                                                                            <  2:1694602/150‑1 (MQ=255)
                                           ttCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCt                                                                                                            <  2:5426931/150‑1 (MQ=255)
                                            tCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTg                                                                                                           <  1:6625379/150‑1 (MQ=255)
                                            tCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTg                                                                                                           <  2:3820701/150‑1 (MQ=255)
                                               gCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                        <  2:1973236/150‑1 (MQ=255)
                                                  tCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATtat                                                                                                     <  1:6531196/150‑1 (MQ=255)
                                                  tCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATtat                                                                                                     >  1:4413553/1‑150 (MQ=255)
                                                  tCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATtat                                                                                                     >  1:1284146/1‑150 (MQ=255)
                                                  tCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATtat                                                                                                     >  1:3640339/1‑150 (MQ=255)
                                                   cATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATtata                                                                                                    <  1:4754611/150‑1 (MQ=255)
                                                    aTGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTatat                                                                                                   <  2:7779399/150‑1 (MQ=255)
                                                     tGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATAtt                                                                                                  >  2:7582079/1‑150 (MQ=255)
                                                      gCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTg                                                                                                 <  2:8164326/150‑1 (MQ=255)
                                                        aGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAg                                                                                               <  2:2182779/150‑1 (MQ=255)
                                                        aGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAg                                                                                               <  1:1512077/150‑1 (MQ=255)
                                                         gATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAgg                                                                                              <  2:2310823/150‑1 (MQ=255)
                                                          aTGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGc                                                                                             <  2:1215098/150‑1 (MQ=255)
                                                               ccACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTc                                                                                        >  1:4298219/1‑150 (MQ=255)
                                                                cacaGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCa                                                                                       >  2:5953097/1‑150 (MQ=255)
                                                                      tAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTcc                                                                                 >  1:7265016/1‑150 (MQ=255)
                                                                       aaaGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTccc                                                                                <  1:8573323/150‑1 (MQ=255)
                                                                       aaaGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGACGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTccc                                                                                <  2:2566653/150‑1 (MQ=255)
                                                                          gTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCAtt                                                                             >  2:4901473/1‑150 (MQ=255)
                                                                           tGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCAttt                                                                            >  1:7965447/1‑150 (MQ=255)
                                                                             aaGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTAt                                                                          >  2:6439405/1‑150 (MQ=255)
                                                                                 tACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCag                                                                      <  1:2128083/150‑1 (MQ=255)
                                                                                  aCTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCaga                                                                     <  2:277980/150‑1 (MQ=255)
                                                                                  aCTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCaga                                                                     <  2:3640339/150‑1 (MQ=255)
                                                                                   cTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAGTATATTGAGGCAAGTCACCCTCCCATTTATTCagag                                                                    >  2:7081046/1‑150 (MQ=255)
                                                                                   cTGCCTTTAAGCTCGATGGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCagag                                                                    <  1:465598/150‑1 (MQ=255)
                                                                                      ccTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaa                                                                 <  1:3278170/150‑1 (MQ=255)
                                                                                      ccTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaa                                                                 >  1:3177376/1‑150 (MQ=255)
                                                                                       cTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTTTAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaa                                                                <  2:1469123/150‑1 (MQ=255)
                                                                                        tttAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaaa                                                               <  2:6510162/150‑1 (MQ=255)
                                                                                        tttAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaaa                                                               >  1:7774427/1‑150 (MQ=255)
                                                                                        tttAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaaa                                                               <  1:6420929/150‑1 (MQ=255)
                                                                                        tttAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaaa                                                               <  1:6327447/150‑1 (MQ=255)
                                                                                         ttAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAg                                                              >  1:1421478/1‑150 (MQ=255)
                                                                                               tCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATAAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTTATGCAAGTCACCCTCCCATTTATTCATAGTAAAAGTatata                                                        >  2:5642490/1‑149 (MQ=255)
                                                                                                   ttGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCtgt                                                    <  2:5250401/150‑1 (MQ=255)
                                                                                                   ttGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCtgt                                                    <  1:7413419/150‑1 (MQ=255)
                                                                                                   ttGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCtgt                                                    <  2:35523/150‑1 (MQ=255)
                                                                                                      gCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCAGGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGAt                                                 >  2:7442632/1‑150 (MQ=255)
                                                                                                                cATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCt                                       <  2:3202516/150‑1 (MQ=255)
                                                                                                                cATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCt                                       <  1:1165246/150‑1 (MQ=255)
                                                                                                                  tCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGa                                     >  1:4843235/1‑150 (MQ=255)
                                                                                                                    cTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGAtt                                   >  2:2816661/1‑150 (MQ=255)
                                                                                                                    cTGGCCGTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGAtt                                   <  1:949455/150‑1 (MQ=255)
                                                                                                                      ggCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCa                                 <  2:5806129/150‑1 (MQ=255)
                                                                                                                       gCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAGTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCAt                                <  2:1857435/150‑1 (MQ=255)
                                                                                                                        ccTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATa                               <  1:3403197/150‑1 (MQ=255)
                                                                                                                        ccTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATa                               <  1:3588347/150‑1 (MQ=255)
                                                                                                                        ccTTACTCAATTAGCTATTAATCATCGCCAGCGCGAGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATa                               <  1:3845473/150‑1 (MQ=255)
                                                                                                                         cTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAg                              <  2:5527253/150‑1 (MQ=255)
                                                                                                                         cTTACACAATTAGCTATTAATCATCCCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAg                              <  1:5735752/150‑1 (MQ=255)
                                                                                                                           tACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCt                            >  2:4666580/1‑150 (MQ=255)
                                                                                                                              tCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTaa                         >  1:1016728/1‑150 (MQ=255)
                                                                                                                                aaTTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTaaaa                       >  2:8360854/1‑150 (MQ=255)
                                                                                                                                   tAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCTTGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGCGTATAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAAt                    <  1:1974678/150‑1 (MQ=255)
                                                                                                                                      cTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAcc                 <  1:5356287/150‑1 (MQ=255)
                                                                                                                                       tATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAccc                >  1:5932399/1‑150 (MQ=255)
                                                                                                                                       tATTAATCATCGCCAGCGAGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCAGCCTCACATTTATTCAGATTAATATTCTATTCTGTGATAAATGGCGCTGTTTCATAGCTTataatttacgc                >  2:3766590/1‑143 (MQ=255)
                                                                                                                                        gaacatcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCt               >  2:8498696/5‑150 (MQ=255)
                                                                                                                                        aTTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTg                                      >  2:2041566/1‑127 (MQ=255)
                                                                                                                                        aTTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCt               >  1:6998497/1‑150 (MQ=255)
                                                                                                                                         ttAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCtt              <  2:6955288/150‑1 (MQ=255)
                                                                                                                                         ttAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCtt              >  1:6715977/1‑150 (MQ=255)
                                                                                                                                           aaTCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGt            <  2:7281575/150‑1 (MQ=255)
                                                                                                                                            atcatcGCCCGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtc           >  1:5005867/1‑150 (MQ=255)
                                                                                                                                            atcatcGCCCGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtc           >  2:437309/1‑150 (MQ=255)
                                                                                                                                             tcatcGCCCGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtca          >  1:5238459/1‑150 (MQ=255)
                                                                                                                                             tcatcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtca          >  2:7140231/1‑150 (MQ=255)
                                                                                                                                              catcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgc                                          <  2:6599553/117‑1 (MQ=255)
                                                                                                                                              catcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaa         <  1:6020310/150‑1 (MQ=255)
                                                                                                                                                tcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaatc       >  1:1800983/1‑150 (MQ=255)
                                                                                                                                                tcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaatc       >  2:4797639/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGTCAATCAACCC  >  NC_000913/1225966‑1226264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: