BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,286,965	A→T	7.1%	intergenic (+439/+101)	narI → / ← rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,286,965	0	A	T	7.1%	827.5 / 5.8	323	intergenic (+439/+101)	narI/rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand): ref base A (121/153); new base T (7/15); total (141/182)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGT  >  NC_000913/1286817‑1287114

 cATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGAGCTTCGCCCAGCGGGTCGTTGCCGTAGGCAAAGCTGTAACGCTCACg                                                                                                                                                     >  2:11601/1‑150 (MQ=14)

  aTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGc                                                                                                                                                    >  1:3817475/1‑150 (MQ=255)

  aTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGc                                                                                                                                                    >  2:2810853/1‑150 (MQ=255)

   tCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                                                   >  2:5262366/1‑150 (MQ=255)

      ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCGGACGCTCGa                                                                                                                                                >  1:8463186/1‑150 (MQ=255)

          ccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                                            >  1:4936170/1‑150 (MQ=255)

          ccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGATTCGCCCTGCGGGGCGTTGCCTGCGGCACCGCTCTCTCGCTGGCGCGCGAGCCg                                                                                                                                            >  2:5548136/1‑150 (MQ=14)

             ctgtggCAGATTATTTGATTGGGGATTAGTTTGAGAATTCCTGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAc                                                                                                                                         <  2:6086663/146‑1 (MQ=14)

             cTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAc                                                                                                                                         <  1:8659658/150‑1 (MQ=255)

                  gCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCCCTTCGCCCTACGGGTCGATTCCAGCGGCAACGATCTCACGCTGGCGCACGAGACGAAACTTAg                                                                                                                                    >  2:4216766/1‑150 (MQ=11)

                   cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGACGAACCTTGGt                                                                                                                                   >  2:4826140/1‑150 (MQ=255)

                   cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCCACCCGCTCACCCGGACCCACGAGCCGCACCATAGt                                                                                                                                   >  1:3083551/1‑150 (MQ=14)

                        tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAATTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCTGACCCAAGAGCCGAAACAAAGTCGAAg                                                                                                                              >  1:5990323/1‑150 (MQ=14)

                         atTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGc                                                                                                                             >  2:3069430/1‑150 (MQ=255)

                          tttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCACCTCTGAGAGCTACGCACATCCCGCCGGTGCCAGACGCAAACATCACACGATGGCGCTCGCCCCGAACCGTATTCGAAGcg                                                                                                                            >  1:1457272/1‑149 (MQ=9)

                              aTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtctc                                                                                                                        <  1:5644347/150‑1 (MQ=255)

                                  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                    >  2:6317728/1‑150 (MQ=255)

                                       tCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGTAAGGATTTCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                               <  1:4089543/150‑1 (MQ=255)

                                       tCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCACATCCTTccc                                                                                                               >  2:7674619/1‑150 (MQ=255)

                                        cGTTTGTGTATTCGGTGTATTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                              <  2:679957/150‑1 (MQ=11)

                                          tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCATCTCATCCTTCCCCGc                                                                                                            >  2:6960377/1‑150 (MQ=255)

                                           ttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACACACGTCTGAGAGGTTCGCGCTTCGGGTCGTTGGCTTGGGAAACGCTCTATGGCAGGCTGTAGATCGGAGGCTTCGTCGAAGCTTGTCATACTTCCCCggg                                                                                                           >  2:7185256/1‑148 (MQ=2)

                                             gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCaag                                                                                                         >  1:5052442/1‑148 (MQ=255)

                                              agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                        <  1:3247138/150‑1 (MQ=255)

                                                aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                      >  2:1123974/1‑150 (MQ=255)

                                                 aTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                     <  2:1292303/150‑1 (MQ=255)

                                                  ttCCGGGGCTTCTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCGCTCTCCCAGACGCTCGACCCGAACCATAGTCGCAGCTTCTCATCCTTCCCCGCATGGGCAg                                                                                                    >  2:8398688/1‑150 (MQ=14)

                                                   tCCGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                   <  2:8071195/150‑1 (MQ=255)

                                                      ggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTTCGGCAACGCTCTATCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAata                                                                                                >  2:4305271/1‑150 (MQ=255)

                                                          tttaaggaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTg                                                                                            >  2:5147068/8‑150 (MQ=255)

                                                          cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAACGCACTCTCCCTGACGCACCAGGCGAACCATAGTCGAAACATATCAAACAAACCCCCAAGGGCAGAataaatt                                                                                            >  1:5741038/1‑146 (MQ=2)

                                                                  aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGa                                                                                    <  1:6816755/150‑1 (MQ=39)

                                                                   gTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAt                                                                                   <  1:6098998/150‑1 (MQ=39)

                                                                    tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCGGCGGCAACCCACACTCGCAGTCGCACCAGCCCGACCCTAGTAGAACCGTCACAACCATCCCCCCACGGGCAGAAAAATTGATTTCGGCtt                                                                                  >  1:2757412/1‑150 (MQ=2)

                                                                    tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAtt                                                                                  <  1:7992398/150‑1 (MQ=21)

                                                                    tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAtt                                                                                  <  2:869440/150‑1 (MQ=39)

                                                                    tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCGCTCTCGCGGACGCGCGAGCCCAAACTAAGTCGCAGCATCTAAAACATCCCCCCAAGGGCAGAATATTTTAtttagggtt                                                                                  >  1:3344011/1‑143 (MQ=2)

                                                                        ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCt                                                                              <  1:2810853/150‑1 (MQ=40)

                                                                        ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTGGGAGCGGAGTCggg                                                                              >  2:8389665/1‑148 (MQ=11)

                                                                         gtggtggtgGAAGGATGACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                             <  1:4447427/150‑1 (MQ=255)

                                                                         gtggtggGGGCAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                             <  1:1398457/150‑1 (MQ=255)

                                                                         gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                             <  1:2483481/150‑1 (MQ=255)

                                                                         gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                             <  1:1922907/150‑1 (MQ=255)

                                                                          tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGa                                                                                                                                                       >  2:7879636/1‑75 (MQ=31)

                                                                           ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                           <  1:1169117/150‑1 (MQ=255)

                                                                           ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGCGGCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                           <  2:4459117/150‑1 (MQ=255)

                                                                            gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                                          <  1:2315956/150‑1 (MQ=255)

                                                                             tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga                                                                         >  2:3530726/1‑150 (MQ=255)

                                                                             tggGGGAAGGATTACTCAGCGCTGCGCGCGTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGTGGCGAACCTTAGTCCTAGCTTCTCATCCTTCCCCGCATGGGGAGAATATTTGATTGCGGATTCGCTTgaga                                                                         <  2:7599905/150‑1 (MQ=14)

                                                                                gggAAGGCTTACTCTGCGCTGCGCGCTTAGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCACTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGtt                                                                      <  2:5855353/150‑1 (MQ=14)

                                                                                 ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                                     <  1:3653344/150‑1 (MQ=255)

                                                                                   aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                                   >  2:1808012/1‑150 (MQ=255)

                                                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                                  <  1:5262366/150‑1 (MQ=255)

                                                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                                  <  1:5786824/150‑1 (MQ=255)

                                                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                                  <  1:3530726/150‑1 (MQ=255)

                                                                                          aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACttt                                                            <  2:5063195/150‑1 (MQ=255)

                                                                                              aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGTGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGac                                                        >  2:8011772/1‑149 (MQ=255)

                                                                                              aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAc                                                                                                                                         <  1:7145519/69‑1 (MQ=40)

                                                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                       <  2:6113193/150‑1 (MQ=255)

                                                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                       <  2:2854347/150‑1 (MQ=255)

                                                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                       <  2:3083551/150‑1 (MQ=255)

                                                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                       <  1:2968404/150‑1 (MQ=255)

                                                                                               gcgcTGCGCGCGTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                       <  1:4185331/150‑1 (MQ=255)

                                                                                                  cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                       <  1:6959713/147‑1 (MQ=255)

                                                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                                                   <  1:7508495/150‑1 (MQ=255)

                                                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                                                   >  2:5044698/1‑150 (MQ=255)

                                                                                                    gcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAt                                                  >  2:4664758/1‑150 (MQ=255)

                                                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtg                                                 <  1:239823/150‑1 (MQ=255)

                                                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtg                                              <  2:5377807/150‑1 (MQ=255)

                                                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCATCTCAACCATCCCCGCATGGGCAGAATATTTGATTGCGGATTAGCTTGGGAGTTAAGGGACATTTGAAAGTGAtggtg                                              >  2:1017902/1‑150 (MQ=14)

                                                                                                            cGCCCTTCGGGTCGTTGCCTGCGGCAACGCCCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                          <  1:5400966/150‑1 (MQ=255)

                                                                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                                       <  1:1123974/150‑1 (MQ=255)

                                                                                                                 ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTCGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                     <  2:6341494/150‑1 (MQ=255)

                                                                                                                 ttCGGGTCGTGGCCGGCGGCAACGCTCTCTCGCGGGCGCTCGAGCCGAACCGTCGTCGAAGCATATCATCCGTCCCCGCAGGGGCCGAATATTTGATTGCGGATTCGCGGGGCAGGTCAGGGGCTTTGGAAAGTGATGggggggggggcc                                     >  2:6912293/1‑148 (MQ=9)

                                                                                                                 ctCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                     <  2:1974938/149‑1 (MQ=255)

                                                                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGCGCCGAACCTTTGTCGAAGCGTCTCATCCTTCCCCGCTTGGGCATAATATTTGATTGCGGATTAGCTTGAGAGTTCAGGGAATTTTGAAAGTGATGGTGGTGGGGGAAg                                    <  2:3422311/150‑1 (MQ=14)

                                                                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                    <  1:2168934/150‑1 (MQ=255)

                                                                                                                   gagagcGTTGCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                   >  1:7869790/6‑150 (MQ=17)

                                                                                                                     ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCAAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCATAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                 <  2:5657328/150‑1 (MQ=255)

                                                                                                                     ggTCGTTGCCTGCGGCAACGCTCACTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTATCATCCTTCCCCCCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACGTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                 >  2:7424043/1‑150 (MQ=255)

                                                                                                                      gTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTTCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                >  2:507273/1‑150 (MQ=255)

                                                                                                                       tCGTTGCGTGAGGCTTCGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                               <  1:6626132/150‑1 (MQ=14)

                                                                                                                       tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                               <  1:631502/150‑1 (MQ=255)

                                                                                                                       tCGTTGCCTGCGGCAACGCTCTCGCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGCTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttc                               <  2:2429571/150‑2 (MQ=255)

                                                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                             <  1:4732398/150‑1 (MQ=255)

                                                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                           >  2:2084202/1‑150 (MQ=255)

                                                                                                                             ccgcaggCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTGAAAGTGATGGGGGTGGGGGAAGGGTTACTCacc                         >  1:8245725/6‑148 (MQ=17)

                                                                                                                             ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAAGTGTGCAACTTAGGGTGGTGGGGGAGGGAATACTCAgc                         >  2:5745105/1‑150 (MQ=17)

                                                                                                                                  ggCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                    >  2:6026582/1‑150 (MQ=255)

                                                                                                                                    cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGGGAGTTCAGTGAAGTTTGAATCTTATTGTGGTGGTGGAAGGATTACTCAGCTCTtcgc                  >  2:1565547/1‑150 (MQ=17)

                                                                                                                                          tctctcGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg            <  2:6426330/150‑1 (MQ=255)

                                                                                                                                          tctatcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                       <  1:2654035/75‑1 (MQ=255)

                                                                                                                                          tctatcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                       >  2:2654035/1‑75 (MQ=255)

                                                                                                                                           ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTAAGCGCTGCGCGCTTCGc           >  2:135767/1‑150 (MQ=255)

                                                                                                                                                gCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc      <  1:3027442/150‑1 (MQ=255)

                                                                                                                                                  tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                        <  1:4551285/98‑1 (MQ=255)

                                                                                                                                                   gACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTATTCAGCGCTGCGCGCCTCACCCATCggg   >  2:294614/1‑150 (MQ=255)

                                                                                                                                                   gACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCggg   >  1:6256555/1‑150 (MQ=255)

                                                                                                                                                    aCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGt  >  2:3865992/1‑150 (MQ=255)

                                                                                                                                                    aCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGt  <  1:4305271/150‑1 (MQ=255)

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGT  >  NC_000913/1286817‑1287114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`