Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,159 T→A 5.0% noncoding (78/171 nt)
Q6H (CAA→CAT
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1590TA 5.0% 828.5 / 3.1 329noncoding (78/171 nt)
Q6H (CAA→CAT
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base T (113/165);  new base A (23/2);  total (160/169)
Fisher's exact test for biased strand distribution p-value = 5.10e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

GGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTT  >  NC_000913/1287012‑1287308
                                                                                                                                                   |                                                                                                                                                      
ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTg                                                                                                                                                      <  1:7674619/150‑1 (MQ=255)
  cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCGCGAGTCGAACCATGGt                                                                                                                                                    >  1:7958682/1‑150 (MQ=255)
   aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTc                                                                                                                                                   <  1:6960377/150‑1 (MQ=255)
   aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGCCGTTGCCTGGGGCAACGGTCTCTCGCTTGCGCTCGAGTCGAACCTTCGTc                                                                                                                                                   >  2:4738556/1‑150 (MQ=14)
    gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                                  >  2:4131454/1‑150 (MQ=255)
     aaTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGa                                                                                                                                                 <  1:135241/150‑1 (MQ=255)
        atTTGATTTCGGATTCGCGTGAGAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGc                                                                                                                                              <  1:5373288/150‑1 (MQ=255)
         tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCt                                                                                                                                             >  2:7719037/1‑150 (MQ=255)
          ttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCtt                                                                                                                                            >  2:4976406/1‑150 (MQ=255)
           tGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGTCGAACCATGGTCGAAGCTtc                                                                                                                                           >  1:5271824/1‑150 (MQ=255)
               tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                                       >  2:5633579/1‑150 (MQ=255)
               tGCGGATTCGCTAGAGATTTCTTGGTCTTTTGATAGTGATGGTGGTGTGGGAAGTTATGATGTGTGCTGCGCGCATCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                                       <  1:51509/150‑1 (MQ=14)
                tctgATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                                      <  1:3478689/147‑1 (MQ=255)
                     ttCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACGGTCTCTCGCTTTCGCTCGAGTCGAACCGTAGTCGCAGCGTCACAACCGTcc                                                                                                                                 >  1:3919929/1‑150 (MQ=14)
                        gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                              >  2:6020663/1‑150 (MQ=255)
                        gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCACGCGCTCGCGCGGGCGCACGCCGCGAACCTTGGTCGAAGCGTCACATCCATccccc                                                                                                                              >  2:3268168/1‑149 (MQ=14)
                          ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCCCTGGCGCGCGAGGCGAACCATGGTCGGAGCGTCTAATCCATccccccc                                                                                                                            >  1:2591940/1‑147 (MQ=14)
                          ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCGCTCGCTGGCGCTAGAGTCGAACCTTGGTCGAATCTTCTCATACATCCCCGCa                                                                                                                            >  2:858311/1‑150 (MQ=14)
                          ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCACAAGCTGGCCCACCAGTCGAACCATGGTAGAAGCATCACAACCATACCCCCa                                                                                                                            >  1:6784534/1‑150 (MQ=14)
                              gagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                                       >  2:928995/1‑150 (MQ=255)
                                    aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCACATCCTTCCCCCCAAGGGCAGaaa                                                                                                                 >  2:232167/1‑149 (MQ=255)
                                           tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAACGATCTCTCGCTTTCGCTCGAATCGAACCGTGGTCGAAGCTTCTCAACCATCCCCGCAGTGGGCGAAAATTTCAt                                                                                                          >  2:2702020/1‑150 (MQ=11)
                                              gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCACCGCTCTATCGCAGGCGCACCACTCGAACCAAGGTCGAAGCATAACATCCAAACCCCCAAGGGCAGAATAATTAAATGc                                                                                                       >  1:4324239/1‑150 (MQ=11)
                                               aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                                      <  1:4976406/150‑1 (MQ=39)
                                                aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGGTATCTCGCTTTCGCTAGAGTCGAACCGTTGTCGAAGGTTCTCAACCTTCCCCGCAGAGTCAGAAAATTaaaaatctg                                                                                                     >  1:1251235/1‑144 (MQ=11)
                                                   tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTCACGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTCACAACCATACCCGCATGGGCAGAATAATTAATTGCGGAtt                                                                                                  >  2:3402923/1‑150 (MQ=11)
                                                   tGATGGTGGTGGGGGAAGGATTACTCAGAGCTGAGCGCTTCGCCCTTCGGGTCGTTGGCTGACGCAAACCACTAAAGCTGGCGCACGAGTCCAAACGTGGTCGAATCATATAAACCTTAACCGCCGGGGCACAATATTTAAATTGGGAtt                                                                                                  >  2:51886/1‑150 (MQ=2)
                                                       ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                              <  1:8398473/150‑1 (MQ=37)
                                                         tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCAAACTTCCCCCCAAGGGCAGAATATTTAATTGCGGATTCGGTgg                                                                                            >  2:4253674/1‑150 (MQ=11)
                                                          ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                                           <  1:3386017/150‑1 (MQ=39)
                                                           gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCATCCCCCCCTGGGCAGAATATTTAATTGCGGATTCGGTGGGa                                                                                          >  2:5440872/1‑150 (MQ=11)
                                                                ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTc                                                                                     >  2:2072912/1‑150 (MQ=255)
                                                                 gtaGGATTTCTCAGCGCTGCGAGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                                    <  1:5745084/148‑1 (MQ=255)
                                                                  aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                                   <  1:8195313/150‑1 (MQ=255)
                                                                     gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                >  2:6257513/1‑150 (MQ=255)
                                                                     gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCCGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                <  1:1488128/150‑1 (MQ=255)
                                                                        tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                             <  2:2757448/150‑1 (MQ=255)
                                                                           tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAGTATTTATTTTCGGGTTGGTTGGGACGTTGAGGGAGTtgtg                                                                          >  2:7331279/1‑150 (MQ=14)
                                                                              gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                                       <  1:6020663/150‑1 (MQ=255)
                                                                                  tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGTTTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                                   <  1:928995/150‑1 (MQ=255)
                                                                                  tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                                   <  1:7378111/150‑1 (MQ=255)
                                                                                     gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCTAGGCTCTCTCGCGGGCGCTCGAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGTAAGTGAtgg                                                                <  2:8111908/150‑1 (MQ=17)
                                                                                       gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtg                                                              <  1:5865979/150‑1 (MQ=255)
                                                                                             cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTc                                                                                                                                  <  1:4478080/77‑1 (MQ=34)
                                                                                              ccTCCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                                                       <  2:5156092/150‑1 (MQ=255)
                                                                                                ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                     <  1:6394301/150‑1 (MQ=255)
                                                                                                gtcgtttCGTTGACTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                     <  1:4684948/144‑1 (MQ=255)
                                                                                                gtCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                     <  1:7788747/149‑1 (MQ=255)
                                                                                                gtCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                     <  2:2732435/149‑1 (MQ=255)
                                                                                                 tCGGGTCGTTGCCTGCGGAAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                                    <  1:2084202/150‑1 (MQ=255)
                                                                                                 tCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCAGTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                                    <  1:5541921/150‑1 (MQ=255)
                                                                                                   agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAGTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGGCTTTTGGAAGGGGTGGGGGTGGGGGAAGGa                                                  >  1:2492542/5‑150 (MQ=14)
                                                                                                    tgTCGTTTGCTGCTGCTAAGCTCTCTCGCTGTCGCTCGAGTCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                                 <  1:155883/149‑1 (MQ=14)
                                                                                                     agcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                                >  2:8196981/3‑150 (MQ=255)
                                                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTc                                                                                     >  2:6792240/1‑110 (MQ=255)
                                                                                                         ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                                       <  1:8573768/123‑1 (MQ=255)
                                                                                                           gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAcc                                          >  2:5716203/1‑150 (MQ=255)
                                                                                                            ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                                         >  2:2387666/1‑150 (MQ=255)
                                                                                                            ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAATTTAGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAACCt                                         >  1:6313994/1‑150 (MQ=255)
                                                                                                            ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAAATGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGTGGGTGGTGGGGGAAGGATTCGAACCt                                         >  1:6568421/1‑150 (MQ=255)
                                                                                                             cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                                        >  2:2858243/1‑150 (MQ=255)
                                                                                                                cGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                                          >  1:1759220/1‑113 (MQ=255)
                                                                                                                 ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCgga                                    >  2:3829421/1‑148 (MQ=255)
                                                                                                                 ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                                    >  2:7903000/1‑150 (MQ=255)
                                                                                                                    aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTc                                 >  2:1852011/1‑150 (MQ=255)
                                                                                                                     aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                                <  2:8149748/150‑1 (MQ=255)
                                                                                                                      cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                               <  2:3046303/150‑1 (MQ=255)
                                                                                                                      cGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                                       >  1:4219054/1‑78 (MQ=21)
                                                                                                                       gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTGGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                              >  2:8408740/1‑150 (MQ=255)
                                                                                                                        ctctctCGCTGGCGCTAGAGTCGAACCTTGGTCGAAGCTTATCAACCATCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTCAGGGACATTTGAAATTGATGGTTGTGGGGGAAGGATTAGAACCTTAGAAgaagaag                             >  1:4792106/1‑144 (MQ=17)
                                                                                                                         tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtg                                                              <  2:7965416/116‑1 (MQ=255)
                                                                                                                         tctcacGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCAACCATACCCGCAAGGGCAGAATATTTAAATGCGGATTCGTTGGGAAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCATCGAAGTCGATGa                            >  1:646159/1‑150 (MQ=18)
                                                                                                                          cgataggtGGCTCTCTAGCCGAACGACGGTCGTAGGTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAc                           <  2:4516336/143‑1 (MQ=18)
                                                                                                                           tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                          >  2:7272241/1‑150 (MQ=255)
                                                                                                                             tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc                        <  1:2780433/150‑1 (MQ=255)
                                                                                                                                 tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt                    <  1:2988348/150‑1 (MQ=255)
                                                                                                                                 tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAgg                                                                                  <  1:3207028/88‑1 (MQ=11)
                                                                                                                                  ggCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt                   <  2:1277545/150‑1 (MQ=255)
                                                                                                                                   gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAttt                  >  2:1956315/1‑150 (MQ=255)
                                                                                                                                    cgcTCGAGTAGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTa                 <  1:3377447/150‑1 (MQ=255)
                                                                                                                                     gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc                <  1:1040635/150‑1 (MQ=255)
                                                                                                                                     gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                             <  1:19938/73‑1 (MQ=25)
                                                                                                                                      cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa               <  1:5065333/150‑1 (MQ=255)
                                                                                                                                      cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa               <  1:8018391/150‑1 (MQ=255)
                                                                                                                                       tcaaGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                                      <  2:3155340/59‑1 (MQ=25)
                                                                                                                                       tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGACGATGACGGCAGATTTACAg              >  2:1639383/1‑150 (MQ=255)
                                                                                                                                       tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTAAATTGCGGAGTCGATGGGAAGTTCAGGGACTTTTGAACGAGTTGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCTATGACGGCAGATTTTCAg              >  2:4592069/1‑150 (MQ=21)
                                                                                                                                         gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc            <  1:232167/150‑1 (MQ=255)
                                                                                                                                         gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAATTAATTGCGGATTCGTTGGGAAGTTCAGGGACGTTTGAACGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc            >  1:7575375/1‑150 (MQ=255)
                                                                                                                                         gAGTCGAACCTTGGTCGAAGCTTCTAAAACTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAACCTTCGAAGTCGATTAAGGCAGATTTACAGta            >  1:3433290/1‑149 (MQ=21)
                                                                                                                                           gTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg          <  1:60933/150‑1 (MQ=255)
                                                                                                                                           gTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg          <  2:3344011/150‑1 (MQ=255)
                                                                                                                                            tCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGc         >  2:5181533/1‑150 (MQ=255)
                                                                                                                                             cGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCt        <  2:5999042/150‑1 (MQ=255)
                                                                                                                                             cGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCt        <  1:1083182/150‑1 (MQ=255)
                                                                                                                                               aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTcc      >  2:1710547/1‑150 (MQ=255)
                                                                                                                                                  cTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                              >  2:7075709/1‑59 (MQ=255)
                                                                                                                                                  cTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                              <  1:7075709/59‑1 (MQ=255)
                                                                                                                                                  cTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGTAGGATTCGAACCTTCGAAGTCGATGTCGGCAGATTTACAGTCTGCTCCCtt   >  1:7256864/1‑150 (MQ=255)
                                                                                                                                                  cTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCtt   >  2:112913/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTT  >  NC_000913/1287012‑1287308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: