Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,324,838 T→A 5.5% Y31* (TAT→TAA) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,324,8380TA 5.5% 1389.8 / 48.9 395Y31* (TAT→TAA) ‡yciQenhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (189/183);  new base A (10/12);  total (200/195)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01

Only 100 of 398 total aligned reads displayed.

GGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGA  >  NC_000913/1324689‑1324987
                                                                                                                                                     |                                                                                                                                                     
 gCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATg                                                                                                                                                      >  2:547423/1‑150 (MQ=255)
   gTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGTTAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaa                                                                                                                                                    <  2:5559933/150‑1 (MQ=255)
   gTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaa                                                                                                                                                    >  1:5000910/1‑150 (MQ=255)
   gTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaa                                                                                                                                                    >  1:3329881/1‑150 (MQ=255)
    tAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaag                                                                                                                                                   >  2:8496869/1‑150 (MQ=255)
      ggTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaagaa                                                                                                                                                 <  1:1375722/150‑1 (MQ=255)
       gTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGaagaag                                                                                                                                                >  1:8620555/1‑150 (MQ=255)
        tGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAgg                                                                                                                                               <  2:7872546/150‑1 (MQ=255)
        tGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAgg                                                                                                                                               >  1:6018427/1‑150 (MQ=255)
         gATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAggg                                                                                                                                              <  1:6578453/150‑1 (MQ=255)
           tgtgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAGGGAt                                                                                                                                            <  2:800158/150‑1 (MQ=255)
           tgtgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGAt                                                                                                                                            <  2:1677646/150‑1 (MQ=255)
             tgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGAtct                                                                                                                                          >  1:4789048/1‑150 (MQ=255)
               aaGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAGGGAtctcc                                                                                                                                        <  2:3790525/150‑2 (MQ=255)
               aaGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCg                                                                                                                                        <  1:3785649/150‑1 (MQ=255)
               aaGCCTTTCTTATAACAAATTGCGGTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCg                                                                                                                                        <  1:51085/150‑1 (MQ=255)
                 gCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGct                                                                                                                                      <  1:7827898/150‑1 (MQ=255)
                  ccTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGctc                                                                                                                                     >  1:8706939/1‑150 (MQ=255)
                     ttCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCttt                                                                                                                                  <  2:2925631/150‑1 (MQ=255)
                     ttCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCttt                                                                                                                                  >  2:8234667/1‑150 (MQ=255)
                       cTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGa                                                                                                                                <  2:4208564/150‑1 (MQ=255)
                        ttATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGAt                                                                                                                               <  2:4764940/150‑1 (MQ=255)
                        ttATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGAt                                                                                                                               >  2:7572080/1‑150 (MQ=255)
                        ttATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGAt                                                                                                                               >  1:6485532/1‑150 (MQ=255)
                           taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                            <  2:7107829/150‑1 (MQ=255)
                           taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                            <  1:1606005/150‑1 (MQ=255)
                            aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg                                                                                                                           >  2:3075628/1‑150 (MQ=255)
                             aCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa                                                                                                                          <  1:4565801/150‑1 (MQ=255)
                              cAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGaa                                                                                                                         <  1:3847578/150‑1 (MQ=255)
                               aaaTTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAg                                                                                                                        <  2:1454362/150‑1 (MQ=255)
                                    gCGTGCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGATGGGATCTCGCTCTTTGATGTTGAAGCCAct                                                                                                                   <  1:3494682/150‑1 (MQ=255)
                                       ttCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                                                                                                >  1:2572649/1‑150 (MQ=255)
                                         ccACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCa                                                                                                              >  2:5943688/1‑150 (MQ=255)
                                           aCGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAAc                                                                                                            >  2:2401066/1‑150 (MQ=255)
                                           aCGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAAc                                                                                                            >  1:5839458/1‑150 (MQ=255)
                                            cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAAcc                                                                                                           <  2:3581632/150‑1 (MQ=255)
                                             ggATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa                                                                                                          <  2:4789048/150‑1 (MQ=255)
                                                 ggAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg                                                                                                      <  2:5398938/150‑1 (MQ=255)
                                                  gAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg                                                                                                     >  2:7370439/1‑150 (MQ=255)
                                                   aaGACTTTGGCTGGGTAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATggg                                                                                                    <  1:7645151/150‑1 (MQ=255)
                                                       cTATGGCAGGGAAGTTTCGCTGCATCTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGc                                                                                                >  2:5634427/1‑150 (MQ=255)
                                                         aTGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTc                                                                                              <  1:1033642/150‑1 (MQ=255)
                                                          tGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATACCTTCGTATGAAGAAGGGATCTCGCTCTTTTATGTTGAATCCACACTGCAACCAGATGGGGTGCTCg                                                                                             >  1:7378648/1‑150 (MQ=255)
                                                             cAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACa                                                                                          >  2:700996/1‑150 (MQ=255)
                                                             cAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACa                                                                                          <  1:5455692/150‑1 (MQ=255)
                                                             cAGGGAAGTATCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACa                                                                                          <  2:4847709/150‑1 (MQ=255)
                                                                 gAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCaa                                                                                      <  2:6027721/150‑1 (MQ=255)
                                                                    gTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGa                                                                                   >  2:4423515/1‑150 (MQ=255)
                                                                     tttCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaa                                                                                  >  2:2663292/1‑150 (MQ=255)
                                                                     tttCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaa                                                                                  >  2:7247468/1‑150 (MQ=255)
                                                                            gCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTc                                                                           <  1:7268310/150‑1 (MQ=255)
                                                                                tttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCAttt                                                                       >  1:4903710/1‑150 (MQ=255)
                                                                                tttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCAttt                                                                       <  1:2464468/150‑1 (MQ=255)
                                                                                        tGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGgcgc                                                               <  1:5778512/150‑1 (MQ=255)
                                                                                         gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGcgcg                                                              >  2:4615082/1‑150 (MQ=255)
                                                                                             gCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTAAGGCGCGAAAt                                                          >  1:4013156/1‑150 (MQ=255)
                                                                                              cAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCATATTATGAAGAAGGGATCTCGCTCTTTGATGTTTAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATc                                                         >  1:1872051/1‑150 (MQ=255)
                                                                                               aGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATca                                                        <  1:8101790/150‑1 (MQ=255)
                                                                                                gggCTGTTTGTATCATCTCTAAGTTAGGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag                                                       <  2:1897905/150‑1 (MQ=255)
                                                                                                  gCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcagca                                                     <  1:7219852/150‑1 (MQ=255)
                                                                                                  gCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcagca                                                     <  2:3901587/150‑1 (MQ=255)
                                                                                                  gCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcagca                                                     <  1:2826596/150‑1 (MQ=255)
                                                                                                   cTGGTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCACCCAGATGGGGTGCTCGACATCAAAGAACATCTTCATTTTCAGGCGCGACATcagcag                                                    >  2:8476070/1‑150 (MQ=255)
                                                                                                      tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGAtt                                                 >  1:7202661/1‑150 (MQ=255)
                                                                                                         gTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAg                                              <  2:8280658/150‑1 (MQ=255)
                                                                                                          tATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGc                                             >  1:6237405/1‑150 (MQ=255)
                                                                                                           atcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCa                                            <  2:5208685/150‑1 (MQ=255)
                                                                                                           atcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCa                                            >  2:6529832/1‑150 (MQ=255)
                                                                                                            tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCAc                                           >  2:3417953/1‑150 (MQ=255)
                                                                                                              atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAACATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACgg                                         >  1:3195098/1‑150 (MQ=255)
                                                                                                              atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACgg                                         <  1:2205561/150‑1 (MQ=255)
                                                                                                               tctcTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGc                                        >  1:5251340/1‑150 (MQ=255)
                                                                                                               tctcTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGc                                        <  2:2142822/150‑1 (MQ=255)
                                                                                                                ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                       >  1:5064236/1‑150 (MQ=255)
                                                                                                                ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                       <  2:2236958/150‑1 (MQ=255)
                                                                                                                ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                       <  2:1225265/150‑1 (MQ=255)
                                                                                                                ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                       <  2:1047905/150‑1 (MQ=255)
                                                                                                                   tAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCtttt                                    >  2:7708587/1‑150 (MQ=255)
                                                                                                                     aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTAt                                  <  1:413231/150‑1 (MQ=255)
                                                                                                                      gTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATc                                 <  2:505994/150‑1 (MQ=255)
                                                                                                                      gTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATc                                 <  1:4417301/150‑1 (MQ=255)
                                                                                                                      gTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATc                                 >  2:2430332/1‑150 (MQ=255)
                                                                                                                       ttATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCg                                >  2:6327854/1‑150 (MQ=255)
                                                                                                                        tATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGt                               <  2:8653997/150‑1 (MQ=255)
                                                                                                                           gCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGAt                            >  1:5540109/1‑150 (MQ=255)
                                                                                                                             aGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTCTCGTGAttt                          <  2:7419570/150‑1 (MQ=255)
                                                                                                                                 aaCACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCa                      <  2:2043419/150‑1 (MQ=255)
                                                                                                                                  acacGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCAc                     >  1:694514/1‑150 (MQ=255)
                                                                                                                                    acGGAGATCCCTTCTTATGAATAATGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTAATTTTCAGGCGCGAAATCAGCAGATTAATCACGGCTTTTATCGTGATTTACCACGa                   >  1:8237429/1‑150 (MQ=255)
                                                                                                                                    acGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGa                   >  1:4238319/1‑150 (MQ=255)
                                                                                                                                         gaTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATg              <  2:5802422/150‑1 (MQ=255)
                                                                                                                                          aTCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATgg             >  2:4031996/1‑150 (MQ=255)
                                                                                                                                           tCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACAt                                                                                         >  2:1074703/1‑73 (MQ=38)
                                                                                                                                           tCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACAt                                                                                         <  1:1074703/73‑1 (MQ=38)
                                                                                                                                           tCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGa            <  2:1794750/150‑1 (MQ=255)
                                                                                                                                             ccTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATg          >  2:6716767/1‑150 (MQ=255)
                                                                                                                                             ccTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATAGTCATTTTCAGGCGCGAAATCAGCAGATTGAGCACGGCTTTTATCGTGAGTTACCACGACTATGGATg          >  2:5274605/1‑150 (MQ=255)
                                                                                                                                                  ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGcc     <  1:530670/150‑1 (MQ=255)
                                                                                                                                                   tATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCt    >  2:2746859/1‑150 (MQ=255)
                                                                                                                                                   tATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCt    <  2:1577654/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGA  >  NC_000913/1324689‑1324987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: