Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,228 G→C 9.8% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2280GC 9.8% 1327.1 / 52.6 419intergenic (‑189/+179)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base G (134/237);  new base C (41/5);  total (176/243)
Fisher's exact test for biased strand distribution p-value = 4.41e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 431 total aligned reads displayed.

TCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351080‑1351377
                                                                                                                                                     |                                                                                                                                                     
tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                      <  2:6672122/150‑1 (MQ=255)
tCAGGCAATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                      <  2:50199/150‑1 (MQ=255)
 cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCagag                                                                                                                                                     <  2:1261487/150‑1 (MQ=255)
  aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg                                                                                                                                                    <  2:3853008/150‑1 (MQ=255)
  aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg                                                                                                                                                    <  1:7211615/150‑1 (MQ=255)
  aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg                                                                                                                                                    <  1:2873808/150‑1 (MQ=255)
  aGGCGATTCGTCGTTTTAGTAAACAGGACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCTGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg                                                                                                                                                    <  1:3208788/150‑1 (MQ=255)
   ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCTCACCCAAACCCTCTCCCCAGAggg                                                                                                                                                   >  2:4521314/1‑150 (MQ=255)
   ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAggg                                                                                                                                                   <  1:295337/150‑1 (MQ=255)
   ggCGATTCGTCGTGGTAGTAAACAGTACGAACAGATAAACGGGTATTATAATCAACCTGGCTGTGAGTAGCTAGAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCCCAggg                                                                                                                                                   >  2:6567526/1‑150 (MQ=255)
        ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGTTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg                                                                                                                                              <  1:1265030/150‑1 (MQ=255)
        ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg                                                                                                                                              <  2:6352780/150‑1 (MQ=255)
         tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                             <  2:3150266/150‑1 (MQ=255)
         tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                             <  1:2415301/150‑1 (MQ=255)
         tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCGCCCCTCTGGGGAGAgg                                                                                                                                             <  1:7453346/150‑1 (MQ=255)
         tcatcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACTCCCCAGAGGGGCGAgg                                                                                                                                             >  1:4342890/4‑150 (MQ=255)
            tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                                                                          <  1:5893306/150‑1 (MQ=255)
            tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATAAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTACCCGCACCCAAACCCTCAACCCCCAGGGGCGAggggc                                                                                                                                          >  1:8492859/1‑149 (MQ=255)
              gtTTTAGTAAACAGTACGCACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCGCCCCTCTGGGGAGAgggttag                                                                                                                                        <  2:8049055/150‑5 (MQ=255)
              gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAcc                                                                                                                                        <  2:2305242/150‑1 (MQ=255)
               ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACACCCCACAGGGGCGAGGGGAccc                                                                                                                                       >  1:4288904/1‑149 (MQ=255)
                  tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                                                                    >  2:4306039/1‑150 (MQ=255)
                  tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                                                                    <  2:4038891/150‑1 (MQ=255)
                   aGTAAACAGTACGAACAGATAAACGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                   <  2:7813543/150‑1 (MQ=255)
                     tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                 >  2:8585664/1‑150 (MQ=255)
                     tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCGAACCCACTCCCCAGCGGGGCGAGGGGGCCGTCTtgt                                                                                                                                 >  2:8332721/1‑150 (MQ=255)
                          aGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                            <  1:516696/150‑1 (MQ=255)
                          aGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                            <  2:616122/150‑1 (MQ=255)
                              cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                        <  1:4926601/150‑1 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                       <  1:5966453/150‑1 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCGAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                       >  2:6794630/1‑150 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCTCTCCCCACAGGGGCGAGGGGACCGTATTTTGGGAATAttt                                                                                                                       >  1:1948330/1‑149 (MQ=255)
                                aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                                                                      >  1:2070213/1‑150 (MQ=255)
                                    gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcc                                                                                                                  <  1:6545471/150‑1 (MQ=255)
                                     aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc                                                                                                                 <  1:1725853/150‑1 (MQ=255)
                                      tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcccc                                                                                                                <  2:5936935/150‑1 (MQ=255)
                                        aaCGGTTATTATAATCATCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCGAg                                                                                                              <  2:5345909/150‑1 (MQ=255)
                                        aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTCACCCCAACCCACGACCCCCGGGGGCGGGGGGGACCGATTGTGCCAATATTGTTACCCCac                                                                                                              >  1:3132489/1‑149 (MQ=255)
                                            gTTATTATAATCAACCTGGCAGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCCCCCTAACCCACACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGGTACCCCAGcaac                                                                                                          >  2:1962368/1‑150 (MQ=39)
                                               attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                       <  2:5647405/150‑1 (MQ=255)
                                                   taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGCCCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                   >  2:5810742/1‑150 (MQ=255)
                                                   taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                   <  1:5387290/150‑1 (MQ=255)
                                                   taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                   >  2:5368367/1‑150 (MQ=255)
                                                    aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc                                                                                                  <  2:4904984/150‑1 (MQ=255)
                                                         aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                                                                             <  2:119748/150‑1 (MQ=255)
                                                          ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACAACCAAAACCACTACCCAGAGGGGCGAGGGGACCCGATTGTGGAAAAATTGTTTCCCCAGCAACAAACAAGCGCCTAc                                                                                            >  2:6880721/1‑150 (MQ=255)
                                                          ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACAACCCACAGGGGCGGGGGGCCCGGATTTTGCAAAAAATGTTAACCCACCCAAAAACAAGCGCATaa                                                                                            >  1:4117926/1‑149 (MQ=255)
                                                             ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                         <  1:922237/150‑1 (MQ=255)
                                                                   gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                   <  1:2584962/150‑1 (MQ=255)
                                                                    aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAc                                                                                  <  2:7633434/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  2:852744/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  2:3381078/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  1:6322118/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCCCACCCCAAACCACACCCCACAGGGGCGAGGGGACCCAAATGTGCAAAAAAATTTAACCCACCAACAAAAACGCACAAAAAGCCCCaaaac                                                                                 >  2:3324658/1‑148 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTGTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  1:6082168/150‑1 (MQ=255)
                                                                        gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCtt                                                                              <  1:3662586/150‑1 (MQ=255)
                                                                         cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCAAACCCACAACCCCCAAGGGCCCGGGGCCCCGAATTTGACAAAATTTTTACACCACCCAAAAACAGGCTCAAAAACCCCCAAAaccaat                                                                             >  1:4759799/1‑147 (MQ=255)
                                                                         cTATAGTGGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTGTCCCCTCACCCTAACCCTCGCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCttt                                                                             <  1:2356031/150‑1 (MQ=255)
                                                                             aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATg                                                                         <  2:6685518/150‑1 (MQ=255)
                                                                             aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATg                                                                         <  2:3501244/150‑1 (MQ=255)
                                                                               ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCGCACCCAAACCCACTCCCCACAGGGGGGGGGGGGCCCTATTGTGGAAATATTTTTTCCCCAGCAACAAAAAGGCTCAGACAGCCCCAAACCCCTTCAAGGc                                                                       >  1:6443597/1‑150 (MQ=255)
                                                                                tGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg                                                                      <  1:3497098/150‑1 (MQ=255)
                                                                                 gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTGCCCGCACCCAAACCCAAACCCCACAGGGGCGCGGGGGCCCGATTTTTCAAAAATTTTTTACCCCCCAAAAAAAACGCGCCAACAGCCCCCAAACCCTTAAAGGCgc                                                                     >  1:7182017/1‑149 (MQ=255)
                                                                                       tCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTGTCCCCAGAGGGGCTAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTg                                                               >  2:4273985/1‑150 (MQ=255)
                                                                                        ccGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTgg                                                              >  2:2136611/1‑150 (MQ=255)
                                                                                           aCCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAACCCAGCAACAAAAAGGCTCAAACAGCCCCAAACCCATTCATGGCGCTGGCTGGGAc                                                           >  2:2647124/1‑150 (MQ=38)
                                                                                             cGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACgg                                                         <  1:816294/150‑1 (MQ=255)
                                                                                               gAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                       <  1:1107676/150‑1 (MQ=255)
                                                                                               gAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCCCACCCAAAACCACACCCCACATGGGCGCGGGGCCCCGATTGTGCAAAAATTGTTTACCCCGCAACAAACAGGCTCAAACAGCCCCGAAACCATTAATGGCGCAGGCGGGGAccggt                                                       >  2:7353681/1‑146 (MQ=255)
                                                                                                   aGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGa                                                   <  2:2889282/150‑1 (MQ=255)
                                                                                                          ggCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                            <  1:1631214/150‑1 (MQ=255)
                                                                                                          ggCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                            <  2:4700335/150‑1 (MQ=255)
                                                                                                            cAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGa                                          <  2:900818/150‑1 (MQ=255)
                                                                                                              gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                        <  1:570152/150‑1 (MQ=255)
                                                                                                               ggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAata                                       <  2:7521854/150‑1 (MQ=255)
                                                                                                                  gggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtc                                    <  2:1457838/150‑1 (MQ=255)
                                                                                                                   ggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtct                                   <  2:1608358/150‑1 (MQ=255)
                                                                                                                    gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCACCAAACAGGCTCATCCCGCCCCTAACCCTTTCATGGCGATGGCGGGGACGGTTCAGACCTTGCCGCATCTtctc                                  >  2:3648564/1‑150 (MQ=38)
                                                                                                                    gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  <  2:7182069/150‑1 (MQ=255)
                                                                                                                    gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  <  1:4886832/150‑1 (MQ=255)
                                                                                                                    gcgcTTTTCCCCTCACCCGAACCCGCTCCCCAGAGGGGCGAGGGGACCGGATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCGTTCCTGGCGAGGGCGGGGACGGTTCCGACCGTGCCGAATATtctc                                  >  2:573209/1‑150 (MQ=38)
                                                                                                                      gcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCa                                <  1:1344399/150‑1 (MQ=255)
                                                                                                                        ttttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                              >  2:2462978/1‑150 (MQ=255)
                                                                                                                        ttttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                              >  2:5534407/1‑150 (MQ=255)
                                                                                                                        ttttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                              >  1:8430965/1‑150 (MQ=255)
                                                                                                                              ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                        >  1:6799383/1‑150 (MQ=255)
                                                                                                                                  aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCt                                                                                     >  1:5584022/1‑85 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCAt                   <  2:1071612/150‑1 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTGTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCAt                   >  1:1030490/1‑150 (MQ=255)
                                                                                                                                      gtccccTCGCCCCTCTGGGGAGAGGGTACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt                >  1:1755283/4‑150 (MQ=255)
                                                                                                                                        aCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAAGATTCTCCCGCACCGGCTCCATGtttg              >  2:7732688/1‑149 (MQ=255)
                                                                                                                                         cccTCGCCCCTCTGGGGAGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTc             >  2:2773911/1‑150 (MQ=255)
                                                                                                                                          ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            <  1:2136611/150‑1 (MQ=255)
                                                                                                                                          ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            >  1:5029252/1‑150 (MQ=255)
                                                                                                                                           ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac           <  2:4342890/150‑1 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac     >  1:7787685/1‑150 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac     >  1:8281539/1‑150 (MQ=255)
                                                                                                                                                   cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca    >  1:5135258/1‑150 (MQ=255)
                                                                                                                                                   cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTCACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca    >  1:385026/1‑150 (MQ=255)
                                                                                                                                                   cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTCACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca    >  1:7133740/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351080‑1351377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: