Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,630 G→C 7.9% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,6300GC 7.9% 1151.6 / 74.5 365pseudogene (1239/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (186/144);  new base C (17/12);  total (209/156)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

ATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466481‑1466779
                                                                                                                                                     |                                                                                                                                                     
aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACg                                                                                                                                                       <  2:6502438/150‑1 (MQ=255)
  tGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCa                                                                                                                                                     >  1:7364802/1‑150 (MQ=255)
  tGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCa                                                                                                                                                     >  1:3709280/1‑150 (MQ=255)
   gATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAg                                                                                                                                                    <  2:7599367/150‑1 (MQ=255)
    atatCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGa                                                                                                                                                   <  2:373575/150‑1 (MQ=255)
     tatCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGAt                                                                                                                                                  <  2:6140497/150‑1 (MQ=255)
       tCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTa                                                                                                                                                >  1:3819181/1‑150 (MQ=255)
        cACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTaa                                                                                                                                               >  1:4501022/1‑150 (MQ=255)
         aCCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAAc                                                                                                                                              <  2:6595297/150‑1 (MQ=255)
           cGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACgg                                                                                                                                            >  1:4662268/1‑150 (MQ=255)
           cGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACgg                                                                                                                                            >  1:8465325/1‑150 (MQ=255)
           cGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACgg                                                                                                                                            <  1:7759978/150‑1 (MQ=255)
           cGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACgg                                                                                                                                            >  1:3211634/1‑150 (MQ=255)
               gACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgat                                                                                                                                        >  2:2441652/1‑150 (MQ=255)
               gACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgat                                                                                                                                        <  2:3399589/150‑1 (MQ=255)
                 cAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgatga                                                                                                                                      <  2:7768632/150‑1 (MQ=255)
                 cAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgatga                                                                                                                                      <  1:1730843/150‑1 (MQ=255)
                  aGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGAc                                                                                                                                     >  1:2030023/1‑150 (MQ=255)
                    cgcAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGc                                                                                                                                   >  2:8420284/1‑150 (MQ=255)
                       aaCGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCAc                                                                                                                                <  2:4983454/150‑1 (MQ=255)
                             ggATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCaaa                                                                                                                          <  1:5739692/150‑1 (MQ=255)
                             ggATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCaaa                                                                                                                          <  1:5265954/150‑1 (MQ=255)
                              gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaac                                                                                                                         >  2:422951/1‑150 (MQ=255)
                                   cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaacaa                                                                                                                    <  2:5333346/150‑1 (MQ=255)
                                    atgggCACCATGACCGTCACCGAGCCGGTGTCCTTGGGTATCCTGGTGGACGGGGACCAGGCGTTGGTGATGATCGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATGAACGGTGATGACGCCAGGGCTaacaacaac                                                                                                                   <  1:2544847/148‑1 (MQ=14)
                                    aaGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGTTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaacaac                                                                                                                   <  2:2720483/150‑1 (MQ=255)
                                     aGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACg                                                                                                                  <  2:5817205/150‑1 (MQ=255)
                                          aCCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTAGTAAACAACGAAGGCGAGAGCGCAATCACCAAAGGTGTCACCGGCAGGCAGATTAACGGTGATGACGACACGGCACTCGACAACGGctat                                                                                                             >  2:3529254/1‑147 (MQ=17)
                                               gACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        <  1:5801746/150‑1 (MQ=255)
                                                    tCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGt                                                                                                     <  2:2112019/148‑1 (MQ=11)
                                                    tCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAAAAACGAAGGCGAGAGCGCAATCACCAAAGGTGGCACCGGCACTCAGATAAACGGTGAAGACGCCACCGCCAAAAACACCGACAAaacaacactta                                                                                                   >  1:3982636/1‑145 (MQ=14)
                                                      aCCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCCGTAGTAAACAACGAAGGCGAGAGCGCAATCACCAACGGAGGCACCGGCACGCAGATCAACGGTGAAGACGCCACCGCGAAAAACAGAGGAAAAACCACAGTTGag                                                                                                 >  1:8647405/1‑149 (MQ=11)
                                                                    cATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                   <  2:7489789/150‑1 (MQ=255)
                                                                     aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                  >  1:831018/1‑150 (MQ=255)
                                                                       cGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCgg                                                                                >  1:5520887/1‑150 (MQ=255)
                                                                       cGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCgg                                                                                >  2:3909895/1‑150 (MQ=255)
                                                                       cGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCgg                                                                                >  2:2998980/1‑150 (MQ=255)
                                                                         gTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGAAGACGCCACCGCGAAAAACAAAGGCAAAACCACCGTTGACGGCAACGATTCCACCGGTa                                                                              >  2:5631976/1‑150 (MQ=18)
                                                                          tATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGATAAACGGTGATGACGCCACCGCAAAAAACAAAGGAAAAACCACAGTTGAAGGCAAGGATTCCACCGGTAc                                                                             >  1:7624675/1‑150 (MQ=17)
                                                                              cAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACTGAGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                         >  1:620511/1‑150 (MQ=255)
                                                                              cAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGac                                                                         >  1:4740402/1‑149 (MQ=255)
                                                                                 gTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAtt                                                                      <  2:7364802/150‑1 (MQ=255)
                                                                                     aCGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTg                                                                  >  1:4869430/1‑150 (MQ=255)
                                                                                      cGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCACCGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTgg                                                                 <  2:4581139/150‑1 (MQ=255)
                                                                                       ggCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAACTTGCTGGc                                                                >  1:6845218/1‑150 (MQ=255)
                                                                                        gCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTCATGACGCCACCGCAAACAACAACGGCAAAACCACCGTTGACGGAAAGGATTCCACCGGTACCGAAATTGCTGGCa                                                               >  1:1202049/1‑150 (MQ=255)
                                                                                                     tgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGt                                                  >  1:8256570/1‑150 (MQ=255)
                                                                                                      gttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTg                                                                     <  2:2916011/130‑1 (MQ=255)
                                                                                                         aataacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCaaa                                                                                                                          >  2:1322637/4‑74 (MQ=0)
                                                                                                         aataacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        >  1:1560656/4‑92 (MQ=0)
                                                                                                         aataacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        <  2:1560656/89‑1 (MQ=0)
                                                                                                         aacaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGAtt                                              <  2:8648655/150‑1 (MQ=255)
                                                                                                          acaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTc                                             >  1:6894986/1‑150 (MQ=255)
                                                                                                          acaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTc                                             >  1:4315609/1‑150 (MQ=255)
                                                                                                            aacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAg                                           <  1:1346692/150‑1 (MQ=255)
                                                                                                             acGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                                >  1:2113815/1‑96 (MQ=40)
                                                                                                              cGAAGGCGAGAGCGCCATCACCAACGGTGGCAACGGCACGCAGATTAACGGAGATGACGCCACGGCAAACAACAACGGCAAAACCTCCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGa                                         >  1:5116694/1‑150 (MQ=255)
                                                                                                                  ggCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGAGATGACGCCACGGCAAACACCAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGc                                     <  2:4099560/150‑1 (MQ=255)
                                                                                                                     gagaGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGAt                                  >  2:5277961/1‑150 (MQ=255)
                                                                                                                     gagaGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGAt                                  <  2:285812/150‑1 (MQ=255)
                                                                                                                     gagaGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGAt                                  <  1:6633065/150‑1 (MQ=255)
                                                                                                                     gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGAt                                  >  2:1749196/1‑150 (MQ=255)
                                                                                                                      agagCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATc                                 >  1:4212500/1‑150 (MQ=255)
                                                                                                                        agCTCCATCACCAACGGTGGCACCGGCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTTCCGGCAATATCGGTAATGTTATTCAGGACGGCGATCTg                               <  1:6279007/150‑1 (MQ=11)
                                                                                                                        agCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTg                               <  1:5179267/150‑1 (MQ=255)
                                                                                                                         gcgcCATCACCACCGGTGGCCCCGGCACGCATATTAACGGTGCTGCCGCCTCGTCAAACAACTCCGGCTAAACCCCCGTTGTCGGCTACGATTCCACGTGTTCGGAAATTGCTGGAAATAACGGGAATGTGATTCAGGACGGCGATCTgg                              <  2:4711560/150‑1 (MQ=17)
                                                                                                                         gcgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCTGGACGGCGATCTgg                              >  1:23493/1‑150 (MQ=255)
                                                                                                                         gcgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTgg                              <  2:6241060/150‑1 (MQ=255)
                                                                                                                         gccccATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTgg                              <  2:8019251/147‑1 (MQ=255)
                                                                                                                          caccATCACCAACGGTGGCACCGGCACGCAGATTAAGGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                            >  2:1469947/3‑87 (MQ=9)
                                                                                                                           ggaaTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGTAAGGTGATTCAGGACGGCGATCTGGAt                            <  1:6963227/147‑1 (MQ=255)
                                                                                                                           gcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGAt                            <  2:2030023/150‑1 (MQ=255)
                                                                                                                            ccATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCGAACAACAACGGCCAAACCACCGTTGGCGGCCAGGATTCCACCGGGACGGAAAGTGCGGGCGATCCCGGGAAGGTGATTCAGGGCGGCGCTCTggcgg                           >  2:7118315/1‑147 (MQ=17)
                                                                                                                            ccATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGATGACGCCACGGCAAACAACAGAGGAAAAACCACAGTTGAGGGAAATGATTCCACCGGTACCTAAATAGCTGGCAATAACGGCAGGGTGATTCAGGAAGGAGGTCTGGATg                           >  2:5792621/1‑150 (MQ=17)
                                                                                                                             cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCaaa                                                                                                                          <  1:2912729/54‑1 (MQ=255)
                                                                                                                             cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGt                          >  1:3444401/1‑150 (MQ=255)
                                                                                                                             cATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGa                                         <  1:4394096/135‑1 (MQ=255)
                                                                                                                              aTCACCAACGGTGGCACCGGCACTCAGATGAACGGTCAGGACGCCACCGCAAACAACAACGGCAAAACCACCGTTGAGGGCAAGGATTCCACCGGTACCGAAATAGCGGGCAATAACGGCAAGGTGATTCTGGACGGAGGCCTGGCTGTc                         >  2:7583000/1‑150 (MQ=14)
                                                                                                                              aTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCACTAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTc                         >  1:3038958/1‑150 (MQ=255)
                                                                                                                              aTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTc                         >  2:6846485/1‑150 (MQ=255)
                                                                                                                                 aCCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAACTTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgc                      >  1:7800502/1‑150 (MQ=255)
                                                                                                                                 aCCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgc                      >  1:991132/1‑150 (MQ=255)
                                                                                                                                 aCCAACGGTGGCACCGGCACCCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAAc                                                          <  1:8011907/114‑1 (MQ=255)
                                                                                                                                  ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTc                                             >  1:1956184/1‑126 (MQ=255)
                                                                                                                                   cAACGGTGGCACCGGCACGCAGATGAACGGTGAGGACGCCACCGCCAACAACACCGGCAAAACCACCGGAGCCGGCGAGCCTGCCCCCGGGACGGACAGTGCTGGCACGACCGGGACGCGGAGGCCGGCCGGCGATCGGGAGGGcggcgg                    >  2:216874/1‑150 (MQ=255)
                                                                                                                                    atcGGTGGCACCGGCACGCAGATGAACGGAGATGACGCCAGGGCAACCCACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggc                   <  2:192356/148‑1 (MQ=17)
                                                                                                                                      cGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                 >  1:7094012/1‑150 (MQ=255)
                                                                                                                                      cGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGATGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                 >  2:5988985/1‑150 (MQ=255)
                                                                                                                                       ggTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggc                <  2:811018/150‑1 (MQ=255)
                                                                                                                                           gCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTgg                                                                 <  2:6911282/97‑1 (MQ=255)
                                                                                                                                             cccGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc          <  1:2441652/149‑1 (MQ=255)
                                                                                                                                              ccGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg         >  2:7264264/1‑150 (MQ=255)
                                                                                                                                              ccGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg         <  1:5988985/150‑1 (MQ=255)
                                                                                                                                               cGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCACTAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg        >  1:2252588/1‑150 (MQ=255)
                                                                                                                                               cGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGaa                                                     >  2:1180424/1‑105 (MQ=255)
                                                                                                                                               cGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg        >  1:1026750/1‑150 (MQ=255)
                                                                                                                                               cGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGAGGTCAGCGGCGGCGGTCACgg        >  2:7719390/1‑150 (MQ=255)
                                                                                                                                               cGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGaa                                                     <  1:2040016/105‑1 (MQ=255)
                                                                                                                                                  cACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt     <  2:4501022/150‑1 (MQ=255)
                                                                                                                                                   aCGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt    <  2:5520887/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466481‑1466779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: