Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,503 G→T 5.4% F78L (TTC→TTA ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5030GT 5.4% 1019.8 / 5.4 332F78L (TTC→TTAykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (163/132);  new base T (7/25);  total (171/161)
Fisher's exact test for biased strand distribution p-value = 3.31e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

TTACCGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAA  >  NC_000913/238354‑238652
                                                                                                                                                     |                                                                                                                                                     
ttaCCGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAg                                                                                                                                                       <  1:6781349/150‑1 (MQ=255)
ttaCCGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTCTTTTTGATTAAg                                                                                                                                                       <  1:8313850/150‑1 (MQ=255)
  aCCGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGaa                                                                                                                                                     <  2:5692059/150‑1 (MQ=255)
   ccGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAt                                                                                                                                                    >  1:326782/1‑150 (MQ=255)
   ccGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAt                                                                                                                                                    >  1:231435/1‑150 (MQ=255)
         gggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtttt                                                                                                                                              >  1:7528650/1‑150 (MQ=255)
           gTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttat                                                                                                                                            >  1:6103956/1‑150 (MQ=255)
              aTAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTtattat                                                                                                                                         <  1:7389698/150‑1 (MQ=255)
               tAATTAGTATTGACCTATCCTGTGACAAAAGGAGATATTAAAGGTGCTATTACGATAGCTATTAGTAAAACTATAAGAGTTAGCTGTTTTGTTATGTCTGTGGCGAAATTGAATAACTTCGTTTTTTTGATTAAGAATGATTTTCTTATc                                                                                                                                        <  1:4063358/150‑1 (MQ=255)
                aaTTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCg                                                                                                                                       >  2:5251052/1‑150 (MQ=255)
                  ttAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgta                                                                                                                                     <  1:4752257/150‑1 (MQ=255)
                   tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                    <  2:8315253/150‑1 (MQ=255)
                      tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTAAGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                 <  1:8503577/150‑1 (MQ=255)
                         tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                              <  2:2689129/150‑1 (MQ=255)
                         tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                              <  1:1404156/150‑1 (MQ=255)
                         tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                              <  1:4019688/150‑1 (MQ=255)
                         tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                              <  2:886879/150‑1 (MQ=255)
                           aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAtt                                                                                                                            >  2:3842770/1‑150 (MQ=255)
                           aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATGAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTGTTGTGATTAAGAATGAGTTTATTAGCggaagtaacagg                                                                                                                            >  2:4707909/1‑146 (MQ=255)
                             atatCCAGAGACAAAAGGAGCTATAAAACGTGCTATTACGATAGCTATTAGTAAAAATATAACACTTAGCTGTAATGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTATTGAATAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                          <  2:6893808/150‑1 (MQ=255)
                              tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                         <  2:7861486/150‑1 (MQ=255)
                              tagccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGAGAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                         <  1:6001582/147‑1 (MQ=255)
                                tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                       >  1:5543959/1‑150 (MQ=255)
                                      gACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATTAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGTTTTTATTATCGTTAGTAAATTTACATGAATAtt                                                                                                                 >  2:5072248/1‑150 (MQ=255)
                                       aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATTAATAttt                                                                                                                >  2:8248584/1‑150 (MQ=255)
                                           aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaa                                                                                                            <  2:3496153/150‑1 (MQ=255)
                                            aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                           <  1:3633223/150‑1 (MQ=255)
                                             ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                          >  2:119716/1‑150 (MQ=255)
                                             ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                          >  1:6148877/1‑150 (MQ=255)
                                                gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                       <  2:2820993/150‑1 (MQ=255)
                                                gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                       <  1:4400802/150‑1 (MQ=255)
                                                      aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                 <  2:2987865/150‑1 (MQ=255)
                                                       aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                >  1:5298145/1‑150 (MQ=255)
                                                       aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                >  2:7411977/1‑150 (MQ=255)
                                                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGa                                                                                             >  1:2381583/1‑150 (MQ=255)
                                                           tGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaa                                                                                            >  2:1125323/1‑150 (MQ=255)
                                                             atATTACGATATCTATTATTAAAAATATAAGAGTTAGCTTTATTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTTATTAATAATTATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAAc                                                                                          <  2:4475927/149‑1 (MQ=255)
                                                              tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                         >  2:4501946/1‑150 (MQ=255)
                                                              tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                         >  2:8577355/1‑150 (MQ=255)
                                                               aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                        <  2:4186032/150‑1 (MQ=255)
                                                                 tACGATAGATATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGaa                                                                                      <  2:7102193/150‑1 (MQ=255)
                                                                  aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                     >  1:1730908/1‑150 (MQ=255)
                                                                          tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCAAAGAAt                                                                             >  2:6969592/1‑150 (MQ=255)
                                                                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                       >  2:1760017/1‑150 (MQ=255)
                                                                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGAATACATTCGTTTTATTGATTAACAATGATTTAATTATCGTAATTAAAAAAACAATAAAATATAAAATTGAAAAACGCAAGAAACAGAATAACATAATaaaaaa                                                                       >  1:1876531/1‑147 (MQ=255)
                                                                                  aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCCAAGCACAGAATCAACATtc                                                                     >  1:7878543/1‑150 (MQ=255)
                                                                                  aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATtc                                                                     >  1:2889199/1‑150 (MQ=255)
                                                                                    aaTATAAGATTTTGCTTTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTGTTTTTTTTTATTAATAATTATTTTTTTATCGTAATTAAAATTACATTAAAATTAAAAATTGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                   <  1:3827429/150‑1 (MQ=255)
                                                                                    aaTATAAGAGTTAGCTGTATTGTTAGGTCTTTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                   <  2:4990698/150‑1 (MQ=255)
                                                                                       tttatttttAGCTTTTTTTTTTTGTCTGGGGTTAATTTGACTACATTCTTTTTTTTTATTAATATTTATTTTATTATCTTAATTAAAATTAAATTAATATTAAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                <  1:1550930/143‑1 (MQ=255)
                                                                                        taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                               <  2:1881456/150‑1 (MQ=255)
                                                                                         atgtgTTAGCTTTTTTGTTTTGTCGGTGGATAATTTGACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCTTAATAAAAATTACATTAATATTAAAAATTTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                              <  1:3142258/146‑1 (MQ=255)
                                                                                         aaGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                              <  2:7738368/150‑1 (MQ=255)
                                                                                         aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                              >  2:2611032/1‑150 (MQ=255)
                                                                                             tttAGCTGTATTTTTTTGTCTGTGGCTAAATTGACTACATTCTTTTTTTTGATTAACAATGATTTTATTACCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaa                                                          <  1:6962161/149‑1 (MQ=255)
                                                                                                aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACAGAAGCACAGAATCAAAATTATCCAAACATAAAat                                                       >  1:6365800/1‑150 (MQ=255)
                                                                                                  cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                     >  2:8108017/1‑150 (MQ=255)
                                                                                                    gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                   >  2:5143329/1‑150 (MQ=255)
                                                                                                    gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                   >  2:6391790/1‑150 (MQ=255)
                                                                                                     tATTGTTTTGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                  <  1:689639/150‑1 (MQ=255)
                                                                                                     tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAACCGAATCACAGAATCAACATTCAACAATCAAAAAATAttta                                                  >  1:7225179/1‑149 (MQ=255)
                                                                                                     tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACACAAAAAACAATCTACCATCATAAAATAATTc                                                  >  1:8128701/1‑150 (MQ=255)
                                                                                                     tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                  >  2:1377166/1‑150 (MQ=255)
                                                                                                      aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAAACGAAGCACAGAAAAAACAATATCCAAAAAAAAAAAAtttac                                                 >  1:294887/1‑148 (MQ=255)
                                                                                                       ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCg                                                >  2:5025067/1‑150 (MQ=255)
                                                                                                         gTTTTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                              <  2:6216715/150‑1 (MQ=255)
                                                                                                         gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATAGTAAGTAAAATTACATGAAAAATTAAAAAGGAAAAACACATGAAAACGAAGCACAGAATCAAAAATAACCAAAAATAAAAAATTTCCGTg                                              >  1:2788420/1‑150 (MQ=255)
                                                                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAAAGACAAGAAAACGAAGCACAGAATCAACAATATCCAAACAAAAAATATTTACGTgg                                             >  1:8609521/1‑150 (MQ=255)
                                                                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGTAATGAAACCGAATCACAGAATGAACATTATACAATCATAAAATATTTGAGTgg                                             >  2:1532553/1‑150 (MQ=255)
                                                                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                             >  2:545294/1‑150 (MQ=255)
                                                                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                             >  2:8439446/1‑150 (MQ=255)
                                                                                                           tttGTCTGTGGCTAAATTTACTACCTTCTTTTTTTTTATTAATATTTATTTTATTATCGTAATTAAAATTAAATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                            <  2:3709758/148‑1 (MQ=255)
                                                                                                                cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTATGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                       <  1:2132460/150‑1 (MQ=255)
                                                                                                                cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGAGGAGCAtt                                       >  2:7262129/1‑150 (MQ=255)
                                                                                                                  gtgGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATGATTTTATTTTAGTAAGTAAAATTAAAAGAATATTTAAAAATGAAAAAGACAAGAAAACCAAGCACAGAAACAACAATATCCAATAATAAAAAAATTAAGTGGAGCAattt                                     >  1:113105/1‑150 (MQ=255)
                                                                                                                   tgGCTAAATCGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                    <  2:2344259/150‑1 (MQ=255)
                                                                                                                      cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                 >  2:5788499/1‑150 (MQ=255)
                                                                                                                       gAACTTGACTACCTTCGTTTTTTTGATCAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                <  2:5942321/150‑1 (MQ=255)
                                                                                                                        aaaTTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                               <  1:5244299/150‑1 (MQ=255)
                                                                                                                        aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                               <  1:1092658/150‑1 (MQ=255)
                                                                                                                             gACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAata                          >  2:4330866/1‑150 (MQ=255)
                                                                                                                              aCTACCTGCGTTTTTTTGATTAAGAATGATTTTCTTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAatat                         <  1:4571459/150‑1 (MQ=255)
                                                                                                                                tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                       >  2:8492582/1‑150 (MQ=255)
                                                                                                                                 aCCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                      <  1:797167/150‑1 (MQ=255)
                                                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                     <  2:2853100/150‑1 (MQ=255)
                                                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                     <  2:7933718/150‑1 (MQ=255)
                                                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                     <  1:5669526/150‑1 (MQ=255)
                                                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTAATATCGTAACTAAAAATACATGAATATTTAAAAAGGAAAACCACATGAAACCCAACCACACAAACAACAAGCTCCAAACAAAAAATAATTACCGGGAGCAATTTATTATTGAATATagag                     >  2:791088/1‑150 (MQ=255)
                                                                                                                                    ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATATACGTGGAGCATTTTATTATTGAATATAGAGGt                   >  1:4618619/1‑150 (MQ=255)
                                                                                                                                      ctttttttttATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                 <  2:3044976/148‑1 (MQ=255)
                                                                                                                                        tttttttGATTAAGAATGATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa               <  1:2017263/150‑1 (MQ=255)
                                                                                                                                        tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa               <  2:2161697/150‑1 (MQ=255)
                                                                                                                                            tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc           >  2:4056291/1‑150 (MQ=255)
                                                                                                                                             ttGATTAAGTATGATTTTATTATCGTAAGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg          <  1:483024/150‑1 (MQ=255)
                                                                                                                                             ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGTATTTTATTATTGAATATAGAGGTTTAACTCCg          >  1:2622356/1‑150 (MQ=255)
                                                                                                                                                aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa       >  1:8204581/1‑150 (MQ=255)
                                                                                                                                                 ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa      >  1:3059763/1‑150 (MQ=255)
                                                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa     >  2:6650597/1‑150 (MQ=255)
                                                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa     <  2:6299229/150‑1 (MQ=255)
                                                                                                                                                     gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa  <  1:516899/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTACCGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAA  >  NC_000913/238354‑238652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: