Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,177 A→C 10.6% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1770AC10.6% 474.9 / 5.5 236intergenic (+112/+261)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (46/129);  new base C (14/12);  total (77/159)
Fisher's exact test for biased strand distribution p-value = 9.96e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326
                                                                                                                                                      |                                                                                                                                                     
tACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCa                                                                                                                                                       >  1:8622285/1‑150 (MQ=255)
 aCTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAg                                                                                                                                                      >  1:1778551/1‑150 (MQ=255)
   tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggg                                                                                                                                                    <  1:1062094/150‑1 (MQ=255)
   tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggg                                                                                                                                                    <  1:8518605/150‑1 (MQ=255)
    ggtggtGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAgggg                                                                                                                                                   <  1:3570013/150‑1 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 <  2:5218120/150‑1 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 <  2:4072575/150‑1 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 >  2:8185766/1‑150 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 <  2:77149/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                               <  1:5195646/150‑1 (MQ=255)
          gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCTGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAgggg                                                                                                                                                   >  2:7190297/1‑144 (MQ=255)
          gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCTGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAgggg                                                                                                                                                   <  1:7190297/144‑1 (MQ=255)
            cTGGCGTATTTAAGATGATATTTTAAAATTAATGAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaa                                                                                                                                           <  1:8361136/150‑1 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCaaa                                                                                                                                          >  1:7258665/1‑149 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCCGCCCAt                                                                                                                                          >  2:2169164/1‑150 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGTAGCGCGCGAGGGGGGGCCaaacacc                                                                                                                                      >  1:2790775/1‑145 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                                      <  1:3351806/150‑1 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                                      <  1:3694601/150‑1 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                                      <  2:4308266/150‑1 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAAGTAATGTCATCAGGTCCGAAAATACCGAGAAGATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCTTGTGCCTTGCTGCAGAGAATCCCTGGCGCCAGGAGCGCGGCGGGGGGCGGcccgcgcc                                                                                                                                       >  1:7423825/1‑144 (MQ=255)
                  tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccg                                                                                                                                     <  1:4224990/150‑1 (MQ=255)
                   aTTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgc                                                                                                                                    <  1:363970/150‑1 (MQ=255)
                   aTTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgc                                                                                                                                    <  1:8392739/150‑1 (MQ=255)
                     ttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATagccgccg                                                                                                                                  >  2:2195819/1‑150 (MQ=255)
                       aaGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCgccgccgcc                                                                                                                                <  2:1111598/150‑1 (MQ=255)
                         gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGcccc                                                                                                                              <  2:645107/150‑1 (MQ=255)
                         gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGcccc                                                                                                                              <  2:6619138/150‑1 (MQ=255)
                            gatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCt                                                                                                                            >  2:5122776/1‑149 (MQ=255)
                             atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCCACGCccccccccccccc                                                                                                                          >  2:2986173/1‑147 (MQ=255)
                               tttttAAAATTAATTACTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGGTCCTGTAATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGCGGAGGGGGCGGCCAATCGCCGCCGCCCCCTGCAc                                                                                                                        <  1:2490965/149‑1 (MQ=38)
                                  ttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCATCGCCGCCGCCCCCAGcccccc                                                                                                                     >  2:8486599/1‑150 (MQ=38)
                                  ttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCCGGcccccc                                                                                                                     >  2:4189755/1‑150 (MQ=255)
                                   tAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAACGCCCCCGCCCCCAGCAccccc                                                                                                                    >  1:5778018/1‑150 (MQ=255)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGCCCAACCCCCCCCCCCCCGCCAccccccc                                                                                                                  >  1:5935261/1‑148 (MQ=255)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGCGGCCCAAAGCCCCCCCCCCCACCACCCCCcgg                                                                                                                 >  2:3396385/1‑150 (MQ=255)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGCGGGCAAAAGCCGCCGCCCCCCGCACCCCCcgg                                                                                                                 >  1:5640603/1‑150 (MQ=255)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATAGCCGCCGCCCCCCGCACCCCCcgg                                                                                                                 >  2:7083130/1‑150 (MQ=255)
                                        ttaattaaTGTCATCAGGTCCGAAATTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATCTAATCACTGGCGCAAGGAGCGCGGAGGGGGCGGCCAAACACCCCCCCCCCCAGCACCCCCCGGca                                                                                                               >  1:2160834/1‑149 (MQ=255)
                                        ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGCGGGCCAACCCCCCCCCCCCCACCCCCCCCCGGcc                                                                                                               >  1:1123306/1‑149 (MQ=255)
                                        ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAACCCCCCCCCCCCCCGCACCCCCCGGcg                                                                                                               >  2:4382419/1‑149 (MQ=38)
                                        ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCAcccccccgct                                                                                                               >  2:732423/1‑145 (MQ=38)
                                         taattactGTCATCAGGGCCGAACAGCACGAGAATATTTCAGTCGCTCATCCTGTTGCGCTCCGGTCATGGGCATGGCTTCATATAATCCCTGGCGCAAGGAGCGCGCAGGGGGCGGCAACTCGCCGCCGCCCCCTGCACCCCCGGGctc                                                                                                              <  2:2462928/150‑1 (MQ=21)
                                         taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGGGGGGCAAAGGCGCCGCCCCCCGCCCCCCCCGGcgc                                                                                                              >  2:6856936/1‑148 (MQ=37)
                                         taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGGGCCCCAAGCCCCCCCCCCCCCCAcccccccgcgc                                                                                                              >  1:7821701/1‑144 (MQ=255)
                                         taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGctc                                                                                                              <  2:1542759/150‑1 (MQ=255)
                                            ttaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTgg                                                                                                           <  2:4550933/150‑1 (MQ=255)
                                             taatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGc                                                                                                          <  2:6331263/150‑1 (MQ=255)
                                             taatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCCCCGCCCCCCGCACCCCCCGGCTCAGGc                                                                                                          >  1:5041771/1‑150 (MQ=37)
                                                  tcatcaGGTCCGAAAATAACGTGAATATTTCAGTGTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGGTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGTGGGCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGGGTACa                                                                                                     >  2:3613232/1‑150 (MQ=25)
                                                   catcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGCGGAGGGGGCGGGCCAAAACCCCCCCCCCCCGCCCCCCCCCGCCCGGGCGacaaa                                                                                                    >  1:7007290/1‑146 (MQ=255)
                                                    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCCCCCCCAGCACCCCCGGGCTCAGGCGAACaaa                                                                                                   >  2:8596925/1‑150 (MQ=255)
                                                       aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCCCCCCCGGCACCCCCCGGCGCTGGCGCACaaaaac                                                                                                >  2:8654259/1‑148 (MQ=25)
                                                           ccGAACATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCGCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgccg                                                                                            <  2:2677202/150‑1 (MQ=255)
                                                              aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTg                                                                                         <  2:613109/150‑1 (MQ=255)
                                                                aaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgcg                                                                                       <  2:7793931/150‑1 (MQ=255)
                                                                    aCGAGAATATTTCTGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAAGCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt                                                                                   <  2:3901341/150‑1 (MQ=255)
                                                                    aCGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCGGTCATGTGCATTGCTTCATATAAGCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt                                                                                   <  2:3756446/150‑1 (MQ=21)
                                                                         aaTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCCCCCCCCCCACCACCCCCCGGCGCTGGCGAACAAAATACCCGCGGCGCGGTGccca                                                                              >  2:582334/1‑149 (MQ=21)
                                                                            tttactgTCTCTCATCCTTTTGCGCTCGTGTCTTTGGCATTGCTACATATAATCAGGGGCGCATGGAGCGCGCTGGGGGCGGCCACGCGCCGCCCCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  1:8133963/144‑1 (MQ=17)
                                                                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:5974427/150‑1 (MQ=255)
                                                                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACGGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:5479981/150‑1 (MQ=255)
                                                                             tttCTGTCTCTCAGCCTGGTGCGCCCGTTTCTTGTGCTTGGCTTAATATAACCCGGGGCGCCCGGTGGGCGCGGGGGGCGGCCAAGCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                          <  1:6756194/150‑1 (MQ=9)
                                                                             tttCAGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCCGGGGCGCATGGTGGGCGCCGGGGGCGGCCACCCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                          <  1:4456165/150‑1 (MQ=18)
                                                                                   tctctcATCGTTTTGCGCTCCGTTCTTGTGCTTTTCTAAATATAATCCGTGGCGCATGGTGCGCGCTGGGGGCGGCAAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                    <  1:7331586/150‑1 (MQ=14)
                                                                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAAGCCGCCCCCCCCAGCACCCCCCGGCGCTTGCGCACAAAAAAGCCGCGGCCCGGTGGCCTCGGCATaaac                                                                    >  2:1264379/1‑147 (MQ=18)
                                                                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                    <  2:5739655/150‑1 (MQ=255)
                                                                                     tctcATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACGGGCGCATGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCt                                                                  <  1:361493/150‑1 (MQ=255)
                                                                                          tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAACGCCCCCCCCCCCCGCACCCCCCCGCGCAGGCCCACAAAAAACCCCCAGCGCCGTGCCCCCCGCGTATACCATAccg                                                             >  2:3498569/1‑148 (MQ=12)
                                                                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                                      <  2:4971619/150‑1 (MQ=255)
                                                                                                 gcgcTCCTGTCATGTGATTTGCTTCATATAATCACTGGCGCACGGCGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                                      <  1:1660587/150‑1 (MQ=17)
                                                                                                   gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                    <  1:4959465/150‑1 (MQ=255)
                                                                                                    cTCCTGTCATGTGCTTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCAAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc                                                   <  1:6246536/150‑1 (MQ=255)
                                                                                                     tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCCCCCCCCCCAGCACCCCAGGGCTCAGGCGAACAAAAACCCCCCAGCCCGGGGCCCGCCGCATAAACCATACGGCAACCCCGTCg                                                  >  1:5208310/1‑150 (MQ=12)
                                                                                                            ttttgctttgcttCATATAAGCCGTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                           <  1:8326515/147‑1 (MQ=14)
                                                                                                             tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                          <  2:7586790/150‑1 (MQ=255)
                                                                                                              gtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                         <  2:1459035/150‑1 (MQ=255)
                                                                                                              gtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                         <  2:1876204/150‑1 (MQ=255)
                                                                                                              gtgCATTGCTTCAGATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                         <  2:5085612/150‑1 (MQ=255)
                                                                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCCGGCACCCCCCGGCTCTGGCGAACAACATCGCCCCGGCGCGGTGCCCGCGGCGGATCCCATACGGCTGCCGGGGCGGGCGCGCggg                                        >  2:6396713/1‑149 (MQ=14)
                                                                                                                 ctttGCTTCATATAAGCACGGGCGCACGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                      <  1:3861360/148‑1 (MQ=255)
                                                                                                                 cATTGCTTCATATAATCACTGGCGCACGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                      <  1:1456831/150‑1 (MQ=255)
                                                                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                      <  1:2330130/150‑1 (MQ=255)
                                                                                                                      cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                 <  2:6265555/150‑1 (MQ=40)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACTAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:7600683/150‑1 (MQ=255)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:2793495/150‑1 (MQ=39)
                                                                                                                         cATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTg                              <  2:7823836/150‑1 (MQ=38)
                                                                                                                              aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaaa                         <  2:4607750/150‑1 (MQ=34)
                                                                                                                               aTCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                        <  1:7572459/150‑1 (MQ=18)
                                                                                                                               aTCACTGGCGCAAGGAGCGCGCACGGGGCCGCCCAACCCCGCCCCCCCCCGCACACCCCGGCGAAGGCCCAAAAAAAACCCCCGGCGCCGTGCCCACCGCGTCTCCCATACGGCGACCAGGGCGGGCCCGCGGGAACAAACCCCTAAAAc                        >  2:5634411/1‑150 (MQ=2)
                                                                                                                                  ccTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                     <  2:5928186/149‑1 (MQ=17)
                                                                                                                                  aCTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                     <  2:61796/150‑1 (MQ=17)
                                                                                                                                   cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  1:6510148/150‑1 (MQ=32)
                                                                                                                                    tGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                   <  2:7247701/150‑1 (MQ=32)
                                                                                                                                        gcAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCa               <  1:6297896/150‑1 (MQ=32)
                                                                                                                                         cAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg              <  1:5579202/150‑1 (MQ=32)
                                                                                                                                         cAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg              <  1:4316702/150‑1 (MQ=32)
                                                                                                                                          aaGGAGCGCGCTGGGGGCGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCATCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc             <  1:3802756/150‑1 (MQ=12)
                                                                                                                                           aGGAGCGGGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  1:3088010/150‑1 (MQ=17)
                                                                                                                                           aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  1:7394862/150‑1 (MQ=32)
                                                                                                                                                  cgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc     <  2:4721585/150‑1 (MQ=32)
                                                                                                                                                      |                                                                                                                                                     
TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: